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The map label for this gene is yfkN [H]

Identifier: 150005254

GI number: 150005254

Start: 3471440

End: 3473182

Strand: Direct

Name: yfkN [H]

Synonym: BVU_2726

Alternate gene names: 150005254

Gene position: 3471440-3473182 (Clockwise)

Preceding gene: 150005253

Following gene: 150005255

Centisome position: 67.23

GC content: 44.92

Gene sequence:

>1743_bases
ATGAAATATAGAATATGTATCAGTGCCCTGCTGTTGTGGGCAGGTATGCAGTTGTCTGCATCCAATAATCAGGAAGAAGT
GGTTATCAAGATTATAGAAACCAGTGATGTGCACGGGAATTTCTTTCCGTATAACTTTATCGAACGGAAAGAATGGAGTG
GAAGTCTTGCCCGTGTTCATTCCTTTGTGAAAGAACAGCGTGAGAAATATGGGGATAATTGCCTGTTGATGGATAATGGG
GATATCCTGCAAGGTCAGCCTACAGCTTACTATTATAATTTTATGGATACCGTGTCTACTCATGTTGCGGCGGATATGAT
GAATTATATGGGATATGTGGTAGGTAATATGGGTAACCATGATGTAGAGACGGGGCATGCGGTGTACGACCGTTGGATAA
GACAGTGCGATTTTCCGGTACTGGGGGCTAATATAATAGACAATGCCACTGGAAAACCTTATCTGAAACCCTACGAGGTG
TTGGAGCGCGACGGAGTGAGGATAGCGGTTCTGGGTATGATTACTCCGGCCATTCCTTCATGGTTGCCGGAGAAATTATG
GAGTGGTCTGCATTTTGAGGAAATGGAGCCCTGCGCACGCAAATGGGTGAAGATTATAAAGGAAAAAGAGAATCCTGATG
TCATTGTGGGATTGTTTCATGCCGGAAAGTCGGGCAACGTGTTGGGACAGGTGGTGGAAGATGCCTCTATGGATGTAGCC
AAACGGGTTCCGGGCTTTGATGTGGTGCTGATGGGGCACGACCATACTAGGGAATGTGTAAAGGTGCAGAATGTGGCAGG
AGACAGTGTGCTGGTCATTGACCCGGCAAACAATGCCAATGTAGTTTCGGATGTGACCCTGACCGTAACAAAGAAAGACG
GGAAAGTAGTGGAGAAGTCGGTGGAAGGTAGATTGGCGGATATGAACAAGTATCCTGTCAGCCGGGAGTTTATGGATAAG
TTTGCCCCTCAATACAAGGCTGTAAATGACTTTGTATCCCGTAAAATCGGTACAATTTCACGTACCATCACAGCGAAGGA
TGCTTATTTCGGTTCTTCTCCTTTCATAGACCTGATTCATCAGTTGCAATTGGAAATTTCGGGTGCTGAAGTGTCTTTCT
GTGCTCCGTTGTCCTTCCGGGCAGAGATTAAAGAAGGAGACATCTATGTGAGTGATATGTTCAATTTGTACAAGTACGAG
AACATGCTGTATGTGATGGAACTTTCGGGCAAGGAAATTAAAGATTTTCTGGAAATGGCATACGCCATCTGGACTAATCA
GATGAAGAGTTCCGAGGACCACCTGATGCTTTTCAAGGAGCCGGTAGAGAAGGGGAAGCGTGCTAATTTCAAGAATTTCA
GCTTTAACTTTGACTCTGCCGCAGGCATTAATTATACGGTGGACGTGACCAAGCCGGCAGGAGAGAAAATCACGATTCAG
AGCATGTCGGACGGGACTCCTTTCAGTATGGATAAGGTTTATAAAGTGGCTTTGAATTCTTACCGTGGGAACGGTGGCGG
AGATTTACTGACTCTTGGAGCTGGAATTTCCAAGGAAGATTTGGCAAAACGTATTATTTTTGCAACCGATAAGGATTTGC
GTTATTATCTGATGCAATATATTGAGCAGGAAAAAGTGCTGCATCCTCATGCTATGCACCAGTGGAAGTTTATTCCGGAG
GAATGGACTGTGCCTGCCGCGAAAAAAGACTATCAATTGTTGTTTGGCGAGGAAAAGAAATAA

Upstream 100 bases:

>100_bases
AGAATCTGGGAGGTAAGATATGGGTGGACAAGGATTATACCGAAGGCGCCCGGTTTGTATTCACGCATCCTTTGACTAAA
AAAGAATAAAGAATAGAGAA

Downstream 100 bases:

>100_bases
ATAGTTGAGCAAGAGAAGGTACATAGTAGCCCCGTTATTACTGCTGGCGCTGTTTTTAACGTATCAGGTAAGCATCACTT
TGTTTACCCACGTTCATATT

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase precursor

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MKYRICISALLLWAGMQLSASNNQEEVVIKIIETSDVHGNFFPYNFIERKEWSGSLARVHSFVKEQREKYGDNCLLMDNG
DILQGQPTAYYYNFMDTVSTHVAADMMNYMGYVVGNMGNHDVETGHAVYDRWIRQCDFPVLGANIIDNATGKPYLKPYEV
LERDGVRIAVLGMITPAIPSWLPEKLWSGLHFEEMEPCARKWVKIIKEKENPDVIVGLFHAGKSGNVLGQVVEDASMDVA
KRVPGFDVVLMGHDHTRECVKVQNVAGDSVLVIDPANNANVVSDVTLTVTKKDGKVVEKSVEGRLADMNKYPVSREFMDK
FAPQYKAVNDFVSRKIGTISRTITAKDAYFGSSPFIDLIHQLQLEISGAEVSFCAPLSFRAEIKEGDIYVSDMFNLYKYE
NMLYVMELSGKEIKDFLEMAYAIWTNQMKSSEDHLMLFKEPVEKGKRANFKNFSFNFDSAAGINYTVDVTKPAGEKITIQ
SMSDGTPFSMDKVYKVALNSYRGNGGGDLLTLGAGISKEDLAKRIIFATDKDLRYYLMQYIEQEKVLHPHAMHQWKFIPE
EWTVPAAKKDYQLLFGEEKK

Sequences:

>Translated_580_residues
MKYRICISALLLWAGMQLSASNNQEEVVIKIIETSDVHGNFFPYNFIERKEWSGSLARVHSFVKEQREKYGDNCLLMDNG
DILQGQPTAYYYNFMDTVSTHVAADMMNYMGYVVGNMGNHDVETGHAVYDRWIRQCDFPVLGANIIDNATGKPYLKPYEV
LERDGVRIAVLGMITPAIPSWLPEKLWSGLHFEEMEPCARKWVKIIKEKENPDVIVGLFHAGKSGNVLGQVVEDASMDVA
KRVPGFDVVLMGHDHTRECVKVQNVAGDSVLVIDPANNANVVSDVTLTVTKKDGKVVEKSVEGRLADMNKYPVSREFMDK
FAPQYKAVNDFVSRKIGTISRTITAKDAYFGSSPFIDLIHQLQLEISGAEVSFCAPLSFRAEIKEGDIYVSDMFNLYKYE
NMLYVMELSGKEIKDFLEMAYAIWTNQMKSSEDHLMLFKEPVEKGKRANFKNFSFNFDSAAGINYTVDVTKPAGEKITIQ
SMSDGTPFSMDKVYKVALNSYRGNGGGDLLTLGAGISKEDLAKRIIFATDKDLRYYLMQYIEQEKVLHPHAMHQWKFIPE
EWTVPAAKKDYQLLFGEEKK
>Mature_580_residues
MKYRICISALLLWAGMQLSASNNQEEVVIKIIETSDVHGNFFPYNFIERKEWSGSLARVHSFVKEQREKYGDNCLLMDNG
DILQGQPTAYYYNFMDTVSTHVAADMMNYMGYVVGNMGNHDVETGHAVYDRWIRQCDFPVLGANIIDNATGKPYLKPYEV
LERDGVRIAVLGMITPAIPSWLPEKLWSGLHFEEMEPCARKWVKIIKEKENPDVIVGLFHAGKSGNVLGQVVEDASMDVA
KRVPGFDVVLMGHDHTRECVKVQNVAGDSVLVIDPANNANVVSDVTLTVTKKDGKVVEKSVEGRLADMNKYPVSREFMDK
FAPQYKAVNDFVSRKIGTISRTITAKDAYFGSSPFIDLIHQLQLEISGAEVSFCAPLSFRAEIKEGDIYVSDMFNLYKYE
NMLYVMELSGKEIKDFLEMAYAIWTNQMKSSEDHLMLFKEPVEKGKRANFKNFSFNFDSAAGINYTVDVTKPAGEKITIQ
SMSDGTPFSMDKVYKVALNSYRGNGGGDLLTLGAGISKEDLAKRIIFATDKDLRYYLMQYIEQEKVLHPHAMHQWKFIPE
EWTVPAAKKDYQLLFGEEKK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=621, Percent_Identity=26.2479871175523, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1786687, Length=243, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652512, Length=409, Percent_Identity=20.2933985330073, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076508, Length=409, Percent_Identity=20.2933985330073, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921980, Length=409, Percent_Identity=20.2933985330073, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 65647; Mature: 65647

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYRICISALLLWAGMQLSASNNQEEVVIKIIETSDVHGNFFPYNFIERKEWSGSLARVH
CCHHHHHHHHHHHCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCHHHCCCCCCHHHHHH
SFVKEQREKYGDNCLLMDNGDILQGQPTAYYYNFMDTVSTHVAADMMNYMGYVVGNMGNH
HHHHHHHHHHCCCEEEEECCCEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DVETGHAVYDRWIRQCDFPVLGANIIDNATGKPYLKPYEVLERDGVRIAVLGMITPAIPS
CCCCCHHHHHHHHHHCCCCEECCHHHCCCCCCCCCCHHHHHHCCCCEEEEEEHHHHHHHH
WLPEKLWSGLHFEEMEPCARKWVKIIKEKENPDVIVGLFHAGKSGNVLGQVVEDASMDVA
HHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
KRVPGFDVVLMGHDHTRECVKVQNVAGDSVLVIDPANNANVVSDVTLTVTKKDGKVVEKS
HHCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHH
VEGRLADMNKYPVSREFMDKFAPQYKAVNDFVSRKIGTISRTITAKDAYFGSSPFIDLIH
HCCCHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHH
QLQLEISGAEVSFCAPLSFRAEIKEGDIYVSDMFNLYKYENMLYVMELSGKEIKDFLEMA
HHHEEECCCEEEEECCCCEEEECCCCCEEHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
YAIWTNQMKSSEDHLMLFKEPVEKGKRANFKNFSFNFDSAAGINYTVDVTKPAGEKITIQ
HHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCEEEEE
SMSDGTPFSMDKVYKVALNSYRGNGGGDLLTLGAGISKEDLAKRIIFATDKDLRYYLMQY
ECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHEEECCCHHHHHHHHH
IEQEKVLHPHAMHQWKFIPEEWTVPAAKKDYQLLFGEEKK
HHHHHCCCCHHHHHCEECCCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MKYRICISALLLWAGMQLSASNNQEEVVIKIIETSDVHGNFFPYNFIERKEWSGSLARVH
CCHHHHHHHHHHHCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCHHHCCCCCCHHHHHH
SFVKEQREKYGDNCLLMDNGDILQGQPTAYYYNFMDTVSTHVAADMMNYMGYVVGNMGNH
HHHHHHHHHHCCCEEEEECCCEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DVETGHAVYDRWIRQCDFPVLGANIIDNATGKPYLKPYEVLERDGVRIAVLGMITPAIPS
CCCCCHHHHHHHHHHCCCCEECCHHHCCCCCCCCCCHHHHHHCCCCEEEEEEHHHHHHHH
WLPEKLWSGLHFEEMEPCARKWVKIIKEKENPDVIVGLFHAGKSGNVLGQVVEDASMDVA
HHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
KRVPGFDVVLMGHDHTRECVKVQNVAGDSVLVIDPANNANVVSDVTLTVTKKDGKVVEKS
HHCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHH
VEGRLADMNKYPVSREFMDKFAPQYKAVNDFVSRKIGTISRTITAKDAYFGSSPFIDLIH
HCCCHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHH
QLQLEISGAEVSFCAPLSFRAEIKEGDIYVSDMFNLYKYENMLYVMELSGKEIKDFLEMA
HHHEEECCCEEEEECCCCEEEECCCCCEEHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
YAIWTNQMKSSEDHLMLFKEPVEKGKRANFKNFSFNFDSAAGINYTVDVTKPAGEKITIQ
HHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCEEEEE
SMSDGTPFSMDKVYKVALNSYRGNGGGDLLTLGAGISKEDLAKRIIFATDKDLRYYLMQY
ECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHEEECCCHHHHHHHHH
IEQEKVLHPHAMHQWKFIPEEWTVPAAKKDYQLLFGEEKK
HHHHHCCCCHHHHHCEECCCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]