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The map label for this gene is ubiE

Identifier: 150005077

GI number: 150005077

Start: 3244614

End: 3245351

Strand: Reverse

Name: ubiE

Synonym: BVU_2542

Alternate gene names: 150005077

Gene position: 3245351-3244614 (Counterclockwise)

Preceding gene: 150005078

Following gene: 150005076

Centisome position: 62.86

GC content: 46.75

Gene sequence:

>738_bases
ATGAACTACCCACAAGAAAAAATCAAGCCCTACAACGCTGACGAAAAGAAAAGCGTCCAAGTAGAGAAAATGTTCGACAA
CATTGCCCCTGCTTACGACCAGTTGAATCATGCCCTTTCATGGAACATTGACAAAAGCTGGCGCCGCAAAGCCATTAACT
GGCTGGAACCTTTTCACCCGCAACATATAATGGATGTAGCCACCGGAACGGGCGATTTTGCCATTCAAGCCTGTCAGACA
CTTCACCCCCAAGAGCTGATAGGTACCGATATATCCGAAGGCATGATGAATGTGGGACGTGAAAAAGTAAAAGCCGCCGG
ACTGGACAGCCGCATCTCTTTCGCTAAGGAAGACTGTACAGCACTCACCTTTCCCGACAAACGCTTTGACGCTATTACCG
TAGCATTCGGTGTACGCAATTTCGAAGACCTTGACAAAGGACTCCGCGAAATGCACCGCGTACTGAAAGACAACGGAAAA
CTAGTCATTTTGGAACTCTCCGAACCCGACTGGTTCCCCATGAAACAGCTATATGCTGTCTATTCTAAAATAGTCATCCC
CACTTTGGGCAAGCTACTCTCTAAAGACCGTAGTGCCTACACTTACCTGCCGCAGTCCATCAAAGCCTTTCCACAAGGCG
AAATAATGACAGACATTATCCGTAAAGCCGGATTCAATCAGGTGAGTTTCAAGCGGTTGACACTAGGTATCTGTACGCTT
TATTTAGCAACGAAATAA

Upstream 100 bases:

>100_bases
TGAAATAAATAAAAATGAAAAATTAAAGATTAAAAGACAGGTACAGGAATCTATCCTCCATCCTCTCCTTTTAATCTTTA
ATTTTCAATTTTAATTAAAC

Downstream 100 bases:

>100_bases
TTTAAAGTATAGAGTTTTATGAAAAAATATGGTTTGATAGGTTATCCGTTAGGACACTCGTTCTCAAAGAACTTTTTCAA
TGAAAAGTTTCATTCGGAAA

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNYPQEKIKPYNADEKKSVQVEKMFDNIAPAYDQLNHALSWNIDKSWRRKAINWLEPFHPQHIMDVATGTGDFAIQACQT
LHPQELIGTDISEGMMNVGREKVKAAGLDSRISFAKEDCTALTFPDKRFDAITVAFGVRNFEDLDKGLREMHRVLKDNGK
LVILELSEPDWFPMKQLYAVYSKIVIPTLGKLLSKDRSAYTYLPQSIKAFPQGEIMTDIIRKAGFNQVSFKRLTLGICTL
YLATK

Sequences:

>Translated_245_residues
MNYPQEKIKPYNADEKKSVQVEKMFDNIAPAYDQLNHALSWNIDKSWRRKAINWLEPFHPQHIMDVATGTGDFAIQACQT
LHPQELIGTDISEGMMNVGREKVKAAGLDSRISFAKEDCTALTFPDKRFDAITVAFGVRNFEDLDKGLREMHRVLKDNGK
LVILELSEPDWFPMKQLYAVYSKIVIPTLGKLLSKDRSAYTYLPQSIKAFPQGEIMTDIIRKAGFNQVSFKRLTLGICTL
YLATK
>Mature_245_residues
MNYPQEKIKPYNADEKKSVQVEKMFDNIAPAYDQLNHALSWNIDKSWRRKAINWLEPFHPQHIMDVATGTGDFAIQACQT
LHPQELIGTDISEGMMNVGREKVKAAGLDSRISFAKEDCTALTFPDKRFDAITVAFGVRNFEDLDKGLREMHRVLKDNGK
LVILELSEPDWFPMKQLYAVYSKIVIPTLGKLLSKDRSAYTYLPQSIKAFPQGEIMTDIIRKAGFNQVSFKRLTLGICTL
YLATK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=267, Percent_Identity=31.8352059925094, Blast_Score=148, Evalue=4e-36,
Organism=Escherichia coli, GI48994983, Length=234, Percent_Identity=35.4700854700855, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17557039, Length=234, Percent_Identity=32.9059829059829, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6323526, Length=243, Percent_Identity=32.0987654320988, Blast_Score=116, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24641702, Length=246, Percent_Identity=35.3658536585366, Blast_Score=160, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_BACV8 (A6L3D5)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299821.1
- ProteinModelPortal:   A6L3D5
- SMR:   A6L3D5
- STRING:   A6L3D5
- GeneID:   5303506
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2542
- NMPDR:   fig|435590.6.peg.2385
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27936; Mature: 27936

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYPQEKIKPYNADEKKSVQVEKMFDNIAPAYDQLNHALSWNIDKSWRRKAINWLEPFHP
CCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
QHIMDVATGTGDFAIQACQTLHPQELIGTDISEGMMNVGREKVKAAGLDSRISFAKEDCT
HHHHHHHCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
ALTFPDKRFDAITVAFGVRNFEDLDKGLREMHRVLKDNGKLVILELSEPDWFPMKQLYAV
EEECCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHH
YSKIVIPTLGKLLSKDRSAYTYLPQSIKAFPQGEIMTDIIRKAGFNQVSFKRLTLGICTL
HHHHHHHHHHHHHHCCCCHHHHCCHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
YLATK
HHCCC
>Mature Secondary Structure
MNYPQEKIKPYNADEKKSVQVEKMFDNIAPAYDQLNHALSWNIDKSWRRKAINWLEPFHP
CCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
QHIMDVATGTGDFAIQACQTLHPQELIGTDISEGMMNVGREKVKAAGLDSRISFAKEDCT
HHHHHHHCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
ALTFPDKRFDAITVAFGVRNFEDLDKGLREMHRVLKDNGKLVILELSEPDWFPMKQLYAV
EEECCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHH
YSKIVIPTLGKLLSKDRSAYTYLPQSIKAFPQGEIMTDIIRKAGFNQVSFKRLTLGICTL
HHHHHHHHHHHHHHCCCCHHHHCCHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
YLATK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA