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The map label for this gene is nfsA [C]

Identifier: 150005070

GI number: 150005070

Start: 3238069

End: 3238698

Strand: Reverse

Name: nfsA [C]

Synonym: BVU_2535

Alternate gene names: 150005070

Gene position: 3238698-3238069 (Counterclockwise)

Preceding gene: 150005071

Following gene: 150005067

Centisome position: 62.73

GC content: 44.6

Gene sequence:

>630_bases
ATGACTGCGGCAACAATCTTTAAAACTCTGACAACTGTTACTCTTTCAATAACATTACTAATTACAGGAGGTTGTAACAA
TATGGAAGCAAAAAAAGAAGAAACAGGAAAGAACACGGCTATAGAAAATATCTTTGCCCGAAAGAGCGTCCGGACCTATA
CACCCCAACCGATAGAAAAAGAAAAAGTGGATTTATTAGTAAAAGCTGCCATGGCTGCACCGACAGCAGTAAACAAGCAG
CCCTGGGCTTTCGTGGTAGTGGATGACCGTAAAGTATTGGATAAACTGGCCGCCGAACTGCCTTATGCCAAGATGACGGC
ACAGGCTCCGCTAGCCATCGTGGTTTGTGGTGATTTAAGCAAAGCCCTGAACGGAGAAACAGATAGGTACTGGATGCTGG
ACTGCTCTGCCGCATCCGAAAATTTGTTGCTGGCAGCAGAATCAATGGGGTTGGGAGCGGTATGGACTGCGGTCTATCCG
GAGAACGACCGCATTGCAAAGGTAAGGTCAGTCTTGTCTTTACCAGACCATATCATTCCTTTCAATTTGATTCCGGTAGG
TTATCCACAACATCGGGAAGAAGCTAAAGACAAATTCAAGACCGAAAATATCCATTATAACAAATGGTGA

Upstream 100 bases:

>100_bases
CGCCATCCATTCGCCCAATGAAAACTTCTCTCTGGATATATTCCGCAAAGGAATTGAAGCTGTGGTAGAATTCCATCTCA
ATTATTAATCAATAATAAAA

Downstream 100 bases:

>100_bases
ACAAAGGTCTAGAAATATAAATTAAGTCGGCTGGTTTAATATAAATCAGCCGACAATCTCTTTCCATTCATCCATAATCA
ATTTTTCTCAATCTCTATTA

Product: putative NADH dehydrogenase/NAD(P)H nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTAATIFKTLTTVTLSITLLITGGCNNMEAKKEETGKNTAIENIFARKSVRTYTPQPIEKEKVDLLVKAAMAAPTAVNKQ
PWAFVVVDDRKVLDKLAAELPYAKMTAQAPLAIVVCGDLSKALNGETDRYWMLDCSAASENLLLAAESMGLGAVWTAVYP
ENDRIAKVRSVLSLPDHIIPFNLIPVGYPQHREEAKDKFKTENIHYNKW

Sequences:

>Translated_209_residues
MTAATIFKTLTTVTLSITLLITGGCNNMEAKKEETGKNTAIENIFARKSVRTYTPQPIEKEKVDLLVKAAMAAPTAVNKQ
PWAFVVVDDRKVLDKLAAELPYAKMTAQAPLAIVVCGDLSKALNGETDRYWMLDCSAASENLLLAAESMGLGAVWTAVYP
ENDRIAKVRSVLSLPDHIIPFNLIPVGYPQHREEAKDKFKTENIHYNKW
>Mature_208_residues
TAATIFKTLTTVTLSITLLITGGCNNMEAKKEETGKNTAIENIFARKSVRTYTPQPIEKEKVDLLVKAAMAAPTAVNKQP
WAFVVVDDRKVLDKLAAELPYAKMTAQAPLAIVVCGDLSKALNGETDRYWMLDCSAASENLLLAAESMGLGAVWTAVYPE
NDRIAKVRSVLSLPDHIIPFNLIPVGYPQHREEAKDKFKTENIHYNKW

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=168, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23108; Mature: 22977

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATIFKTLTTVTLSITLLITGGCNNMEAKKEETGKNTAIENIFARKSVRTYTPQPIEK
CCHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCH
EKVDLLVKAAMAAPTAVNKQPWAFVVVDDRKVLDKLAAELPYAKMTAQAPLAIVVCGDLS
HHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCHHHHCCCCCEEEEEECCCH
KALNGETDRYWMLDCSAASENLLLAAESMGLGAVWTAVYPENDRIAKVRSVLSLPDHIIP
HHHCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCC
FNLIPVGYPQHREEAKDKFKTENIHYNKW
EEEEECCCCCHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
TAATIFKTLTTVTLSITLLITGGCNNMEAKKEETGKNTAIENIFARKSVRTYTPQPIEK
CHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCH
EKVDLLVKAAMAAPTAVNKQPWAFVVVDDRKVLDKLAAELPYAKMTAQAPLAIVVCGDLS
HHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCHHHHCCCCCEEEEEECCCH
KALNGETDRYWMLDCSAASENLLLAAESMGLGAVWTAVYPENDRIAKVRSVLSLPDHIIP
HHHCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCC
FNLIPVGYPQHREEAKDKFKTENIHYNKW
EEEEECCCCCHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]