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The map label for this gene is menE [H]

Identifier: 150004952

GI number: 150004952

Start: 3100488

End: 3101573

Strand: Reverse

Name: menE [H]

Synonym: BVU_2415

Alternate gene names: 150004952

Gene position: 3101573-3100488 (Counterclockwise)

Preceding gene: 150004953

Following gene: 150004951

Centisome position: 60.07

GC content: 48.53

Gene sequence:

>1086_bases
ATGACAAAATTCTGCACCAATAGAAACAAACAGACCATTCTGATAGAAAGCATCCCCTACTCCAACTGGGAAATTGAAAT
GGTAAGAATGAATATCCCTGCCGACAACCGAAGTTTCCGGCAGGAACTTTTCTCCTTTCTTTCCGAATGGTTTGACCCGG
CAGACACCCTGCCGGTACATACTTCAGGCTCCACCGGCAAACCCAAGGAACTGTATGTAGAAAAAGAACGCATGATGGAA
AGCGCCTGCCTCACCTGTTCCTTCCTCGGACTGCAAAAAGAAGATTCCGCCCTGCTTTGTATGCCCTTGCAATACATTGC
CGGAAAAATGGTGGTAATACGTGCCTTGGTAGCGGGACTGGACTTGCTGCCTGTCACCCCCTCCGGACATCCTTTAAAAG
ACCTGACAAAAGCACCCGTCTTTGCCGCCATGATCCCCATGCAAGTCTATAACTCACTGCAAGTTCCCGAAGAGAAAGCG
ATATTGCAACAAATCAGACACCTCATTATCGGAGGCGGTCCTATCGACTCCCAACTGAACGCTGCGTTAAAAGATTTCCC
CCATGCCGTATGGTCTACCTACGGCATGACCGAAACCTTGTCACACATTGCCCTACGCAGACTGAACGGTCCTGAAGCTT
CAGACTGGTACACCCCTTTCGAGAGCATACAGATCCGTCTGAGTAAAGAAAACACCTTGGTGATATACGCTCCCGAAATT
TGCGAAAAAGAACTGGTGACCAATGACATAGCCGAGATAAACGGACAGAACCAATTCCGCATTCTAGGCAGAAAAGACAA
CACGATCAATACAGGAGGAGTAAAAGTGCAGATAGAACAAGTAGAGGCCGCATTGAAAGAACACCTGTCCGTTCCTTTCC
TGATCACCTCCGCCCCCGACGAAAAATTCGGAGAAATCATTGTACTGCTTGCCGAAGGACAACTGCCCGACGACATAGAA
CAGACCTGCACACATCAATTACCTCCCTACTGGCGTCCCAAACGTTTTGTTCCGGTATTCAAACTTCCCCTGACAGAAAC
CGGGAAACCCGACCGTGCCATAGCCAAATTACTGGCGCAAAAATAA

Upstream 100 bases:

>100_bases
TGTTTACCGACAATATAGACTATCCGCTTCACATCGAAGGAGACTGCCTTTGGTTCCACCCCGAAGAACAAGAACCCGAC
TTACTAAACTGGCTGAAACA

Downstream 100 bases:

>100_bases
CTCCGATCCATTCATAAAGGGTGAATTCAAAAACCAATCAAACGGTCTTTTTGAATTCACCCTTCTTTCTGTAAAAAAGA
ATAATTTTCCGTGTTCTCCT

Product: putative O-succinylbenzoate--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTKFCTNRNKQTILIESIPYSNWEIEMVRMNIPADNRSFRQELFSFLSEWFDPADTLPVHTSGSTGKPKELYVEKERMME
SACLTCSFLGLQKEDSALLCMPLQYIAGKMVVIRALVAGLDLLPVTPSGHPLKDLTKAPVFAAMIPMQVYNSLQVPEEKA
ILQQIRHLIIGGGPIDSQLNAALKDFPHAVWSTYGMTETLSHIALRRLNGPEASDWYTPFESIQIRLSKENTLVIYAPEI
CEKELVTNDIAEINGQNQFRILGRKDNTINTGGVKVQIEQVEAALKEHLSVPFLITSAPDEKFGEIIVLLAEGQLPDDIE
QTCTHQLPPYWRPKRFVPVFKLPLTETGKPDRAIAKLLAQK

Sequences:

>Translated_361_residues
MTKFCTNRNKQTILIESIPYSNWEIEMVRMNIPADNRSFRQELFSFLSEWFDPADTLPVHTSGSTGKPKELYVEKERMME
SACLTCSFLGLQKEDSALLCMPLQYIAGKMVVIRALVAGLDLLPVTPSGHPLKDLTKAPVFAAMIPMQVYNSLQVPEEKA
ILQQIRHLIIGGGPIDSQLNAALKDFPHAVWSTYGMTETLSHIALRRLNGPEASDWYTPFESIQIRLSKENTLVIYAPEI
CEKELVTNDIAEINGQNQFRILGRKDNTINTGGVKVQIEQVEAALKEHLSVPFLITSAPDEKFGEIIVLLAEGQLPDDIE
QTCTHQLPPYWRPKRFVPVFKLPLTETGKPDRAIAKLLAQK
>Mature_360_residues
TKFCTNRNKQTILIESIPYSNWEIEMVRMNIPADNRSFRQELFSFLSEWFDPADTLPVHTSGSTGKPKELYVEKERMMES
ACLTCSFLGLQKEDSALLCMPLQYIAGKMVVIRALVAGLDLLPVTPSGHPLKDLTKAPVFAAMIPMQVYNSLQVPEEKAI
LQQIRHLIIGGGPIDSQLNAALKDFPHAVWSTYGMTETLSHIALRRLNGPEASDWYTPFESIQIRLSKENTLVIYAPEIC
EKELVTNDIAEINGQNQFRILGRKDNTINTGGVKVQIEQVEAALKEHLSVPFLITSAPDEKFGEIIVLLAEGQLPDDIEQ
TCTHQLPPYWRPKRFVPVFKLPLTETGKPDRAIAKLLAQK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=294, Percent_Identity=24.4897959183673, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 40587; Mature: 40455

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFCTNRNKQTILIESIPYSNWEIEMVRMNIPADNRSFRQELFSFLSEWFDPADTLPVH
CCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEE
TSGSTGKPKELYVEKERMMESACLTCSFLGLQKEDSALLCMPLQYIAGKMVVIRALVAGL
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
DLLPVTPSGHPLKDLTKAPVFAAMIPMQVYNSLQVPEEKAILQQIRHLIIGGGPIDSQLN
CEEECCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
AALKDFPHAVWSTYGMTETLSHIALRRLNGPEASDWYTPFESIQIRLSKENTLVIYAPEI
HHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEECHHH
CEKELVTNDIAEINGQNQFRILGRKDNTINTGGVKVQIEQVEAALKEHLSVPFLITSAPD
HHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCEEEEHHHHHHHHHHHCCCCEEEECCCC
EKFGEIIVLLAEGQLPDDIEQTCTHQLPPYWRPKRFVPVFKLPLTETGKPDRAIAKLLAQ
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHC
K
C
>Mature Secondary Structure 
TKFCTNRNKQTILIESIPYSNWEIEMVRMNIPADNRSFRQELFSFLSEWFDPADTLPVH
CCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEE
TSGSTGKPKELYVEKERMMESACLTCSFLGLQKEDSALLCMPLQYIAGKMVVIRALVAGL
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
DLLPVTPSGHPLKDLTKAPVFAAMIPMQVYNSLQVPEEKAILQQIRHLIIGGGPIDSQLN
CEEECCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
AALKDFPHAVWSTYGMTETLSHIALRRLNGPEASDWYTPFESIQIRLSKENTLVIYAPEI
HHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEECHHH
CEKELVTNDIAEINGQNQFRILGRKDNTINTGGVKVQIEQVEAALKEHLSVPFLITSAPD
HHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCEEEEHHHHHHHHHHHCCCCEEEECCCC
EKFGEIIVLLAEGQLPDDIEQTCTHQLPPYWRPKRFVPVFKLPLTETGKPDRAIAKLLAQ
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA