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The map label for this gene is ykfB [H]

Identifier: 150004953

GI number: 150004953

Start: 3101570

End: 3102625

Strand: Reverse

Name: ykfB [H]

Synonym: BVU_2416

Alternate gene names: 150004953

Gene position: 3102625-3101570 (Counterclockwise)

Preceding gene: 150004954

Following gene: 150004952

Centisome position: 60.09

GC content: 53.98

Gene sequence:

>1056_bases
ATGTTCCAAACCCGAATTATAAAAAAACAATTACACTTCAAGCAACCGGCAGGTACTTCCAGAGGAGTATATACCACCCG
GAATGTATGGTACATCCTCCTCACCGACACCGGCAGCCGCCACTATGGCGTGGGTGAATGTGCCCCTCTCCCTGCCCTGA
GTTGTGACGATATCCCCTCATACGACGAGGTGTTGGCCACTGCCTGCAAGAACCTGGAAAAGAACGGGGAAATAGACCGG
GAAGCCTTGCTTCCCTATCCGTCCATCCTGTTCGGTATGGAAACCGCCCTGCTGCATTTCCGGGCACGCAGCCTGCAATT
CTGGCACACCCCGTTCAGCAAAGGCAAAGAGGGCATCCCCATCAACGGACTCATCTGGATGGGAAACTTTGACGAGATGT
ACCGGCGCATCGGGGAAAAGATGCAGCAAGGCTTCCGCTGCATCAAACTCAAAATAGGCGCCATCGACTTTGAAAAGGAA
CTGGAGCTGCTGGCGCACATCCGCCGGCATTTCACCCCAGCCCAAATCGAACTGCGTGTAGATGCCAACGGTGCTTTCTC
TCCTGCCGATGCCCTCGAAAAGTTACATCGTCTTTCCGAATTCCAGCTCCATTCCATCGAACAGCCCATCCGTGCGGGGC
AATGGAATGAAATGGCCCGGCTCTGTGCCGCCACCCCCTTTCCCATTGCACTGGATGAAGAGCTGATAGGCATCAACCGG
CGCGACCGGAAAATAGAATTGCTGGAAACCATCCGTCCTCAGTACATTATCCTGAAACCTTCTCTGCACGGAGGAATCAG
CGGCTCCGAAGAATGGATGGAACTGGCGGCTGAAAGAGGCATCGGCTCATGGGTCACTTCCGCTCTGGAGTCCAATATCG
GACTGAATGCCATCGCACAATGGTGTGCCACCTTGCAGCCCGCCCTCCCCCAAGGACTAGGTACCGGCTTGCTGTTTACC
GACAATATAGACTATCCGCTTCACATCGAAGGAGACTGCCTTTGGTTCCACCCCGAAGAACAAGAACCCGACTTACTAAA
CTGGCTGAAACAATGA

Upstream 100 bases:

>100_bases
AGGATTATCCGAAGTTCCCATAATCAGTGGTTCATTTTTCCGGTGGAGGACAACCCAATAGTCCTCCTTGCTTCCCATTA
ACCACTAATCACTAATAAGA

Downstream 100 bases:

>100_bases
CAAAATTCTGCACCAATAGAAACAAACAGACCATTCTGATAGAAAGCATCCCCTACTCCAACTGGGAAATTGAAATGGTA
AGAATGAATATCCCTGCCGA

Product: putative muconate cycloisomerase

Products: O-Succinylbenzoate; H2O. [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MFQTRIIKKQLHFKQPAGTSRGVYTTRNVWYILLTDTGSRHYGVGECAPLPALSCDDIPSYDEVLATACKNLEKNGEIDR
EALLPYPSILFGMETALLHFRARSLQFWHTPFSKGKEGIPINGLIWMGNFDEMYRRIGEKMQQGFRCIKLKIGAIDFEKE
LELLAHIRRHFTPAQIELRVDANGAFSPADALEKLHRLSEFQLHSIEQPIRAGQWNEMARLCAATPFPIALDEELIGINR
RDRKIELLETIRPQYIILKPSLHGGISGSEEWMELAAERGIGSWVTSALESNIGLNAIAQWCATLQPALPQGLGTGLLFT
DNIDYPLHIEGDCLWFHPEEQEPDLLNWLKQ

Sequences:

>Translated_351_residues
MFQTRIIKKQLHFKQPAGTSRGVYTTRNVWYILLTDTGSRHYGVGECAPLPALSCDDIPSYDEVLATACKNLEKNGEIDR
EALLPYPSILFGMETALLHFRARSLQFWHTPFSKGKEGIPINGLIWMGNFDEMYRRIGEKMQQGFRCIKLKIGAIDFEKE
LELLAHIRRHFTPAQIELRVDANGAFSPADALEKLHRLSEFQLHSIEQPIRAGQWNEMARLCAATPFPIALDEELIGINR
RDRKIELLETIRPQYIILKPSLHGGISGSEEWMELAAERGIGSWVTSALESNIGLNAIAQWCATLQPALPQGLGTGLLFT
DNIDYPLHIEGDCLWFHPEEQEPDLLNWLKQ
>Mature_351_residues
MFQTRIIKKQLHFKQPAGTSRGVYTTRNVWYILLTDTGSRHYGVGECAPLPALSCDDIPSYDEVLATACKNLEKNGEIDR
EALLPYPSILFGMETALLHFRARSLQFWHTPFSKGKEGIPINGLIWMGNFDEMYRRIGEKMQQGFRCIKLKIGAIDFEKE
LELLAHIRRHFTPAQIELRVDANGAFSPADALEKLHRLSEFQLHSIEQPIRAGQWNEMARLCAATPFPIALDEELIGINR
RDRKIELLETIRPQYIILKPSLHGGISGSEEWMELAAERGIGSWVTSALESNIGLNAIAQWCATLQPALPQGLGTGLLFT
DNIDYPLHIEGDCLWFHPEEQEPDLLNWLKQ

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 39846; Mature: 39846

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00152 ATPASE_ALPHA_BETA ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQTRIIKKQLHFKQPAGTSRGVYTTRNVWYILLTDTGSRHYGVGECAPLPALSCDDIPS
CCCHHHHHHHHHCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
YDEVLATACKNLEKNGEIDREALLPYPSILFGMETALLHFRARSLQFWHTPFSKGKEGIP
HHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCHHCCCCCCC
INGLIWMGNFDEMYRRIGEKMQQGFRCIKLKIGAIDFEKELELLAHIRRHFTPAQIELRV
CCEEEEECCHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEE
DANGAFSPADALEKLHRLSEFQLHSIEQPIRAGQWNEMARLCAATPFPIALDEELIGINR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEECHHHHCCCC
RDRKIELLETIRPQYIILKPSLHGGISGSEEWMELAAERGIGSWVTSALESNIGLNAIAQ
CCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHH
WCATLQPALPQGLGTGLLFTDNIDYPLHIEGDCLWFHPEEQEPDLLNWLKQ
HHHHHCHHHHHCCCCCEEEECCCCCCEEECCCEEEECCCCCCCHHHHHHCC
>Mature Secondary Structure
MFQTRIIKKQLHFKQPAGTSRGVYTTRNVWYILLTDTGSRHYGVGECAPLPALSCDDIPS
CCCHHHHHHHHHCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
YDEVLATACKNLEKNGEIDREALLPYPSILFGMETALLHFRARSLQFWHTPFSKGKEGIP
HHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCHHCCCCCCC
INGLIWMGNFDEMYRRIGEKMQQGFRCIKLKIGAIDFEKELELLAHIRRHFTPAQIELRV
CCEEEEECCHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEE
DANGAFSPADALEKLHRLSEFQLHSIEQPIRAGQWNEMARLCAATPFPIALDEELIGINR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEECHHHHCCCC
RDRKIELLETIRPQYIILKPSLHGGISGSEEWMELAAERGIGSWVTSALESNIGLNAIAQ
CCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHH
WCATLQPALPQGLGTGLLFTDNIDYPLHIEGDCLWFHPEEQEPDLLNWLKQ
HHHHHCHHHHHCCCCCEEEECCCCCCEEECCCEEEECCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @SUCC01.txt*2-Succinyl-6-Hydroxy-2,4-Cyclohexadiene-1- Carboxylate! [C]

Specific reaction: @SUCC01.txt*2-Succinyl-6-Hydroxy-2,4-Cyclohexadiene-1- Carboxylate! = O-Succinylbenzoate + H2O. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]