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The map label for this gene is folC [H]

Identifier: 150004865

GI number: 150004865

Start: 3004937

End: 3006223

Strand: Reverse

Name: folC [H]

Synonym: BVU_2328

Alternate gene names: 150004865

Gene position: 3006223-3004937 (Counterclockwise)

Preceding gene: 150004866

Following gene: 150004864

Centisome position: 58.22

GC content: 39.32

Gene sequence:

>1287_bases
ATGAATTACGAAGAAACATTGGATTATTTATATAATAGTGCCCCATTATTCCAACATATAGGAAAAGATGCATACAAGGC
AGGGTTAGAAAACACTTATCTTTTAGACAAGTATTTCAACCATCCCCATCGCCAATTCCGAACCATTCATATAGCCGGGA
CCAATGGAAAAGGCTCTTGTTCACATACTTTAGCCGCCATTTTACAATCAGCCGGATACAAGACAGGACTTTACACTTCT
CCACATCTGATAGATTTCCGTGAACGAATCCGCGTCAACGGAATTCCTGTATCCAAAGAATATGTCATAGACTTTGTAGA
AAAACACCGTGCCTTTTTTGAACCTCTGCATCCTTCTTTCTTTGAACTGACTACTGCAATGGCTTTCCACTATTTTGCCC
AAAGCCAAGTAGATGTAGCCATTATAGAAGTCGGTCTGGGAGGACGAATAGATTGTACAAACATCATCTGTCCGGATCTA
TGTGTCATAACCAATATCAGTTTCGACCATATACAATTCTTAGGTAACACATTAGCCAAAATAGCCACAGAGAAGGCCGG
AATCATCAAAGAAAAGACCCCGGTAGTTATCGGTGAAACCACACCCGAAACCAAACCTATATTTACAACCCGTGCTAAAG
AAGTAAACGCTCCTATCTACTTTGCAGAAGAAGAACAGTTATTACACTCCTCCAGCATAAATGAAAAAGGTAAACGAATA
TATCAAACAACCGACTACCTCAATCTGGAAGGCGAACTGGAAGGGCTTTGCCAACTTAAAAACACCAATACACTTTTATC
TGCCATCCGCCTATTAAAACAAGCAGGTTATCAACTTACCGAAAGCAATATACGCAAAGGATTCTCACAAGTATGTGAAC
TCACCGGCCTGATGGGAAGATGGCAAAAATTAGAAAGTGAACCGACTTTAATATGCGATACAGGACATAATGTAGGAGGT
ATTTCGTATATCATAGAACAATTAGAACATCAGAAATATGAACGATTACACATTGTAATAGGTATGGTAAACGACAAGGA
TATCAGTGGAGTATTGTCCATGCTTCCTAAAAATGCCACCTATTATTTTACCAAAGCCAGCGTAAAGCGTGCTTTATCCG
AAAAAGAATTACAAAGCTTAGCGATGCAATCCGGGCTGCACGGAGATACTTATCCTGATGTAGAAACAGCTGTAACAGCC
GCTAAAGAAAAGGCTAACAAAAATGACTTTATTTTTGTTGGAGGAAGCAGCTTTATCGTTGCGGATTTATTAAAATTTCA
CGTTTAG

Upstream 100 bases:

>100_bases
GATTAATTTTTATACTACAAAGTGTCACAGAAAGAATACCAGGTTTTATATCTGCCGGGCATTCTTTTGTGACACTTTGC
GGTAAATAAAAACATTTGCC

Downstream 100 bases:

>100_bases
TCCGTTCACTTTTATAGAAAAAGTTTGGGAATACCGAAGAAAATCTTTTTATTTGCATTAGTTTTATACATAAAATAAAA
AGATTTTCAATAACATGATA

Product: putative folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNYEETLDYLYNSAPLFQHIGKDAYKAGLENTYLLDKYFNHPHRQFRTIHIAGTNGKGSCSHTLAAILQSAGYKTGLYTS
PHLIDFRERIRVNGIPVSKEYVIDFVEKHRAFFEPLHPSFFELTTAMAFHYFAQSQVDVAIIEVGLGGRIDCTNIICPDL
CVITNISFDHIQFLGNTLAKIATEKAGIIKEKTPVVIGETTPETKPIFTTRAKEVNAPIYFAEEEQLLHSSSINEKGKRI
YQTTDYLNLEGELEGLCQLKNTNTLLSAIRLLKQAGYQLTESNIRKGFSQVCELTGLMGRWQKLESEPTLICDTGHNVGG
ISYIIEQLEHQKYERLHIVIGMVNDKDISGVLSMLPKNATYYFTKASVKRALSEKELQSLAMQSGLHGDTYPDVETAVTA
AKEKANKNDFIFVGGSSFIVADLLKFHV

Sequences:

>Translated_428_residues
MNYEETLDYLYNSAPLFQHIGKDAYKAGLENTYLLDKYFNHPHRQFRTIHIAGTNGKGSCSHTLAAILQSAGYKTGLYTS
PHLIDFRERIRVNGIPVSKEYVIDFVEKHRAFFEPLHPSFFELTTAMAFHYFAQSQVDVAIIEVGLGGRIDCTNIICPDL
CVITNISFDHIQFLGNTLAKIATEKAGIIKEKTPVVIGETTPETKPIFTTRAKEVNAPIYFAEEEQLLHSSSINEKGKRI
YQTTDYLNLEGELEGLCQLKNTNTLLSAIRLLKQAGYQLTESNIRKGFSQVCELTGLMGRWQKLESEPTLICDTGHNVGG
ISYIIEQLEHQKYERLHIVIGMVNDKDISGVLSMLPKNATYYFTKASVKRALSEKELQSLAMQSGLHGDTYPDVETAVTA
AKEKANKNDFIFVGGSSFIVADLLKFHV
>Mature_428_residues
MNYEETLDYLYNSAPLFQHIGKDAYKAGLENTYLLDKYFNHPHRQFRTIHIAGTNGKGSCSHTLAAILQSAGYKTGLYTS
PHLIDFRERIRVNGIPVSKEYVIDFVEKHRAFFEPLHPSFFELTTAMAFHYFAQSQVDVAIIEVGLGGRIDCTNIICPDL
CVITNISFDHIQFLGNTLAKIATEKAGIIKEKTPVVIGETTPETKPIFTTRAKEVNAPIYFAEEEQLLHSSSINEKGKRI
YQTTDYLNLEGELEGLCQLKNTNTLLSAIRLLKQAGYQLTESNIRKGFSQVCELTGLMGRWQKLESEPTLICDTGHNVGG
ISYIIEQLEHQKYERLHIVIGMVNDKDISGVLSMLPKNATYYFTKASVKRALSEKELQSLAMQSGLHGDTYPDVETAVTA
AKEKANKNDFIFVGGSSFIVADLLKFHV

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=181, Percent_Identity=39.7790055248619, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI66932990, Length=182, Percent_Identity=39.5604395604396, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1788654, Length=385, Percent_Identity=33.7662337662338, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI71984923, Length=180, Percent_Identity=40, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=180, Percent_Identity=40, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17553150, Length=180, Percent_Identity=40, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323760, Length=199, Percent_Identity=39.6984924623116, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324815, Length=189, Percent_Identity=34.9206349206349, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322718, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641571, Length=186, Percent_Identity=38.1720430107527, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581568, Length=173, Percent_Identity=36.9942196531792, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47990; Mature: 47990

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYEETLDYLYNSAPLFQHIGKDAYKAGLENTYLLDKYFNHPHRQFRTIHIAGTNGKGSC
CCHHHHHHHHHCCCHHHHHHCHHHHHHCCCCCEEHHHHHCCCCCEEEEEEEEECCCCCCH
SHTLAAILQSAGYKTGLYTSPHLIDFRERIRVNGIPVSKEYVIDFVEKHRAFFEPLHPSF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCHH
FELTTAMAFHYFAQSQVDVAIIEVGLGGRIDCTNIICPDLCVITNISFDHIQFLGNTLAK
HHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCHHHHCCCCHHHHHHHHHHHHH
IATEKAGIIKEKTPVVIGETTPETKPIFTTRAKEVNAPIYFAEEEQLLHSSSINEKGKRI
HHHHHCCCEECCCCEEEECCCCCCCCCEEECHHHCCCCEEECCHHHHHHHCCCCHHHHEE
YQTTDYLNLEGELEGLCQLKNTNTLLSAIRLLKQAGYQLTESNIRKGFSQVCELTGLMGR
EEECCEEECCCCHHHHEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
WQKLESEPTLICDTGHNVGGISYIIEQLEHQKYERLHIVIGMVNDKDISGVLSMLPKNAT
HHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHCCCCCE
YYFTKASVKRALSEKELQSLAMQSGLHGDTYPDVETAVTAAKEKANKNDFIFVGGSSFIV
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHH
ADLLKFHV
HHHHHHCC
>Mature Secondary Structure
MNYEETLDYLYNSAPLFQHIGKDAYKAGLENTYLLDKYFNHPHRQFRTIHIAGTNGKGSC
CCHHHHHHHHHCCCHHHHHHCHHHHHHCCCCCEEHHHHHCCCCCEEEEEEEEECCCCCCH
SHTLAAILQSAGYKTGLYTSPHLIDFRERIRVNGIPVSKEYVIDFVEKHRAFFEPLHPSF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCHH
FELTTAMAFHYFAQSQVDVAIIEVGLGGRIDCTNIICPDLCVITNISFDHIQFLGNTLAK
HHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCHHHHCCCCHHHHHHHHHHHHH
IATEKAGIIKEKTPVVIGETTPETKPIFTTRAKEVNAPIYFAEEEQLLHSSSINEKGKRI
HHHHHCCCEECCCCEEEECCCCCCCCCEEECHHHCCCCEEECCHHHHHHHCCCCHHHHEE
YQTTDYLNLEGELEGLCQLKNTNTLLSAIRLLKQAGYQLTESNIRKGFSQVCELTGLMGR
EEECCEEECCCCHHHHEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
WQKLESEPTLICDTGHNVGGISYIIEQLEHQKYERLHIVIGMVNDKDISGVLSMLPKNAT
HHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHCCCCCE
YYFTKASVKRALSEKELQSLAMQSGLHGDTYPDVETAVTAAKEKANKNDFIFVGGSSFIV
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHH
ADLLKFHV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]