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The map label for this gene is pfkA [H]

Identifier: 150004823

GI number: 150004823

Start: 2957969

End: 2959612

Strand: Direct

Name: pfkA [H]

Synonym: BVU_2286

Alternate gene names: 150004823

Gene position: 2957969-2959612 (Clockwise)

Preceding gene: 150004820

Following gene: 150004824

Centisome position: 57.29

GC content: 45.68

Gene sequence:

>1644_bases
ATGACTAAAAGTGCATTACAAATTGCCAGAGCTGCTTATCAGCCCAAACTTCCTAAAGCGTTGAAAGGCGCTGTAAAAGT
AAAGGAAGGTGAACCTACACAGTCAGTTGCTGACCAGGAAGCTATCAAAGCCTTGTTCCCTAACACTTACGGCATGCCGT
TGATTCAGTTCGTTGAAGGCGAAGCTGTCAATATGCCTGCCATCAACGTAGGTGTCATCTTGTCTGGTGGACAGGCTCCC
GGCGGACACAATGTTATTTCCGGTTTGTTCGACGGTATCAAAGCATTGAACAAGGACAGCAAACTGTATGGTTTCATTCT
GGGCCCCGGTGGTTTGGTAGACCATAATTATATGGAACTGACTTCCGATATTATCGACGAATACCGCAATACCGGTGGTT
TCGATATCATCGGTTCGGGACGTACCAAACTGGAAAAGGTAGAACAGTTTGATAAAGGTCTGGAAATCATCAAGGAATTG
GGCATCAAGGCATTGGTAATTATCGGTGGTGATGACTCAAACACAAACGCTTGTGTATTGGCAGAATACTATGCTGCGAA
GAACTGCGGTGTTCAGGTTATCGGTTGTCCGAAGACTATCGACGGTGACTTGAAGAACGAGATGATTGAAACTTCTTTCG
GTTTTGATACGGCTTGTAAGACTTACTCTGAAGTAATCGGTAACATTCAGCGTGACTGTAACTCTGCCCGCAAATACTGG
CACTTTATCAAGCTGATGGGACGTTCGGCTTCTCATATCGCTTTGGAATGTGCTTTGCAGGTTCAACCTAACGTATGTAT
CGTTTCAGAAGAAGTGGAAGAGAAGAACATGTCATTGGATGATGTGGTTACTTACATTGCTCAGGTGGTGGCTAACCGTG
CCGCACAAGGCAATAACTTCGGTACAGTGTTGATACCTGAAGGTTTGATCGAATTCATCCCCGCAATGAAGAGATTGATC
GCTGAATTGAACGACTTCCTGGCTGCTAACGCATCAGAGTTCGCATTGATCAAGAAATCTCACCAACGTGAATATATCAT
CTCCAAGCTTTCTAAGGAAAACTCGGAAATCTATGCTTCTCTGCCCGAAGGCGTTGCACGTCAGCTTACTTTGGATCGTG
ACCCGCACGGAAACGTACAGGTATCACTGATTGAAACAGAAAAGCTGTTGTCTGATATGGTAGCCGTTAAATTGGCTCAG
TGGAAAGAAGAAGGCAAGTATGTAGGTAAATTTGCCGCTCAGCATCACTTCTTCGGTTACGAAGGACGTTGCGCTGCTCC
GTCAAACTTCGATGCTGACTATTGCTACTCGTTGGGTTACACCGCTTCTATGTTGATTGCCAACGGCAAGACCGGTTATA
TGTCTTCTGTACGTAACACTACTGCTCCTGCCGAAGAATGGATTGCAGGTGGTGTGCCTATCACAATGATGATGAACATG
GAACGTCGTCACGGTGAAATGAAACCGGTTATCCAGAAAGCGTTGGTAAAATTGGACGGTGCTCCTTTCAAGGCGTTCGC
CGCACAGCGTGAACGTTGGGCTATTGAAACAGACTATGTATATCCGGGTCCTATCCAGTACTTCGGTCCTACAGAAGTTT
GTGACCAGGCAACCAAGACATTGCAGTTGGAACAAGGAAAATAA

Upstream 100 bases:

>100_bases
ATATTTTTATTTGTATACGTTTTCATATTTGCGAAAAAAGAACGAAATTTGCACGTGAATTATAAATGAAATTATACACC
TTTAAATAGAATAATAGAAT

Downstream 100 bases:

>100_bases
TTTTTTCTTATTTCTATAAAGCATAAAGGAAACCCATCGGGGCTGATTGTAAAACCATTCTCCCGATGGGTTTTATTGCA
TAGTATGTGGGAATAGGTAA

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTKSALQIARAAYQPKLPKALKGAVKVKEGEPTQSVADQEAIKALFPNTYGMPLIQFVEGEAVNMPAINVGVILSGGQAP
GGHNVISGLFDGIKALNKDSKLYGFILGPGGLVDHNYMELTSDIIDEYRNTGGFDIIGSGRTKLEKVEQFDKGLEIIKEL
GIKALVIIGGDDSNTNACVLAEYYAAKNCGVQVIGCPKTIDGDLKNEMIETSFGFDTACKTYSEVIGNIQRDCNSARKYW
HFIKLMGRSASHIALECALQVQPNVCIVSEEVEEKNMSLDDVVTYIAQVVANRAAQGNNFGTVLIPEGLIEFIPAMKRLI
AELNDFLAANASEFALIKKSHQREYIISKLSKENSEIYASLPEGVARQLTLDRDPHGNVQVSLIETEKLLSDMVAVKLAQ
WKEEGKYVGKFAAQHHFFGYEGRCAAPSNFDADYCYSLGYTASMLIANGKTGYMSSVRNTTAPAEEWIAGGVPITMMMNM
ERRHGEMKPVIQKALVKLDGAPFKAFAAQRERWAIETDYVYPGPIQYFGPTEVCDQATKTLQLEQGK

Sequences:

>Translated_547_residues
MTKSALQIARAAYQPKLPKALKGAVKVKEGEPTQSVADQEAIKALFPNTYGMPLIQFVEGEAVNMPAINVGVILSGGQAP
GGHNVISGLFDGIKALNKDSKLYGFILGPGGLVDHNYMELTSDIIDEYRNTGGFDIIGSGRTKLEKVEQFDKGLEIIKEL
GIKALVIIGGDDSNTNACVLAEYYAAKNCGVQVIGCPKTIDGDLKNEMIETSFGFDTACKTYSEVIGNIQRDCNSARKYW
HFIKLMGRSASHIALECALQVQPNVCIVSEEVEEKNMSLDDVVTYIAQVVANRAAQGNNFGTVLIPEGLIEFIPAMKRLI
AELNDFLAANASEFALIKKSHQREYIISKLSKENSEIYASLPEGVARQLTLDRDPHGNVQVSLIETEKLLSDMVAVKLAQ
WKEEGKYVGKFAAQHHFFGYEGRCAAPSNFDADYCYSLGYTASMLIANGKTGYMSSVRNTTAPAEEWIAGGVPITMMMNM
ERRHGEMKPVIQKALVKLDGAPFKAFAAQRERWAIETDYVYPGPIQYFGPTEVCDQATKTLQLEQGK
>Mature_546_residues
TKSALQIARAAYQPKLPKALKGAVKVKEGEPTQSVADQEAIKALFPNTYGMPLIQFVEGEAVNMPAINVGVILSGGQAPG
GHNVISGLFDGIKALNKDSKLYGFILGPGGLVDHNYMELTSDIIDEYRNTGGFDIIGSGRTKLEKVEQFDKGLEIIKELG
IKALVIIGGDDSNTNACVLAEYYAAKNCGVQVIGCPKTIDGDLKNEMIETSFGFDTACKTYSEVIGNIQRDCNSARKYWH
FIKLMGRSASHIALECALQVQPNVCIVSEEVEEKNMSLDDVVTYIAQVVANRAAQGNNFGTVLIPEGLIEFIPAMKRLIA
ELNDFLAANASEFALIKKSHQREYIISKLSKENSEIYASLPEGVARQLTLDRDPHGNVQVSLIETEKLLSDMVAVKLAQW
KEEGKYVGKFAAQHHFFGYEGRCAAPSNFDADYCYSLGYTASMLIANGKTGYMSSVRNTTAPAEEWIAGGVPITMMMNME
RRHGEMKPVIQKALVKLDGAPFKAFAAQRERWAIETDYVYPGPIQYFGPTEVCDQATKTLQLEQGK

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=243, Percent_Identity=31.2757201646091, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 59904; Mature: 59773

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSALQIARAAYQPKLPKALKGAVKVKEGEPTQSVADQEAIKALFPNTYGMPLIQFVEG
CCHHHHHHHHHHCCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHEECC
EAVNMPAINVGVILSGGQAPGGHNVISGLFDGIKALNKDSKLYGFILGPGGLVDHNYMEL
CCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
TSDIIDEYRNTGGFDIIGSGRTKLEKVEQFDKGLEIIKELGIKALVIIGGDDSNTNACVL
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEE
AEYYAAKNCGVQVIGCPKTIDGDLKNEMIETSFGFDTACKTYSEVIGNIQRDCNSARKYW
EEHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HFIKLMGRSASHIALECALQVQPNVCIVSEEVEEKNMSLDDVVTYIAQVVANRAAQGNNF
HHHHHHCCCCCEEEEEEEEEECCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
GTVLIPEGLIEFIPAMKRLIAELNDFLAANASEFALIKKSHQREYIISKLSKENSEIYAS
EEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
LPEGVARQLTLDRDPHGNVQVSLIETEKLLSDMVAVKLAQWKEEGKYVGKFAAQHHFFGY
CHHHHHHHEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
EGRCAAPSNFDADYCYSLGYTASMLIANGKTGYMSSVRNTTAPAEEWIAGGVPITMMMNM
CCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHHCCCCCCHHHHHCCCCCEEEEECC
ERRHGEMKPVIQKALVKLDGAPFKAFAAQRERWAIETDYVYPGPIQYFGPTEVCDQATKT
HHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHE
LQLEQGK
EEECCCC
>Mature Secondary Structure 
TKSALQIARAAYQPKLPKALKGAVKVKEGEPTQSVADQEAIKALFPNTYGMPLIQFVEG
CHHHHHHHHHHCCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHEECC
EAVNMPAINVGVILSGGQAPGGHNVISGLFDGIKALNKDSKLYGFILGPGGLVDHNYMEL
CCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
TSDIIDEYRNTGGFDIIGSGRTKLEKVEQFDKGLEIIKELGIKALVIIGGDDSNTNACVL
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEE
AEYYAAKNCGVQVIGCPKTIDGDLKNEMIETSFGFDTACKTYSEVIGNIQRDCNSARKYW
EEHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HFIKLMGRSASHIALECALQVQPNVCIVSEEVEEKNMSLDDVVTYIAQVVANRAAQGNNF
HHHHHHCCCCCEEEEEEEEEECCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
GTVLIPEGLIEFIPAMKRLIAELNDFLAANASEFALIKKSHQREYIISKLSKENSEIYAS
EEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
LPEGVARQLTLDRDPHGNVQVSLIETEKLLSDMVAVKLAQWKEEGKYVGKFAAQHHFFGY
CHHHHHHHEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
EGRCAAPSNFDADYCYSLGYTASMLIANGKTGYMSSVRNTTAPAEEWIAGGVPITMMMNM
CCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHHCCCCCCHHHHHCCCCCEEEEECC
ERRHGEMKPVIQKALVKLDGAPFKAFAAQRERWAIETDYVYPGPIQYFGPTEVCDQATKT
HHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHE
LQLEQGK
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA