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The map label for this gene is lacZ [H]

Identifier: 150004710

GI number: 150004710

Start: 2803275

End: 2805668

Strand: Direct

Name: lacZ [H]

Synonym: BVU_2171

Alternate gene names: 150004710

Gene position: 2803275-2805668 (Clockwise)

Preceding gene: 150004709

Following gene: 150004711

Centisome position: 54.29

GC content: 47.99

Gene sequence:

>2394_bases
ATGAAAAAGTTTATATCTATATCTTTCTTCACCTGGCTGTTTGTAATGTTCAGCTATCCTCTGTTTGTAGAGGCTGCTAT
GCCAGAGCGGAAACAGTTGTTTGATGACAATTGGCGTTTTAAACTCGGCGATACACCCGATGCCCCTTCACTTGCATATA
ACGATGTCGGTTGGCGAAGCTTGGATCTGCCTCATGACTGGAGTGTTGAAGCCGATTTTGATGCGGAGGTTCCTGCCGGA
AACGATGGTGGCTATCTGCCTACGGGTATTGGATGGTACCGAAAGAAGTTCCACGTAGATAAGGTGATGGAGGGTAAGGA
ACTGCGCCTCTATTTTGAGGGTGTTTATATGAATTCGGAAGTATTTGTCAATGGAGAGCGTGCTGGAGGGCATCCATACG
GTTATTCATCCTATTTCTGTGACATTACTCCTTACCTGCATTTCGGGCAGGAGAACATTGTAGCCGTACGTGTGGATAAC
TCCCAACAGAAGAACTGTCGTTGGTATAGTGGTTCAGGCATCTATCGCCACGTGTGGCTGCTGACTACAGAGGCGATATA
CATTGATGACTGGAGCGTTTATATTCGCCCGACTCAAAAAGAAGGCAGCGATGATTGGAATCTGGACATTGCCATGCGCT
ATATAGACAATGCCCGGACCGGGACAAAACCGGAAATCAAGCATACGATTTATGATGAGGCTGGCAGAGTGCTAGTAACA
TCCGCAAGCGGACACACGAAGCAATCGCTCTCTGTAAGTCATCCGAAACTATGGTCGCCGGATACCCCAAATCTTTATAA
GGTATGTACGCAACTGATTGTTGGAGGCAAAGTGGTAGATGAGGTAACGAATATGACCGGATTCCGAAACATAACTTTCG
ACAGTCAAAAAGGCTTGTTTGTGAATGGTAAACCTATACTTCTGAATGGCGGATGCGTGCATCACGACAACGGCATATTA
GGTGCGTGTTCTTTCGATGCAGCCGAAGCACGCAAGGTGCGATTGTTGAAAGAAGCTGGCTTCAATGCCGTGCGTACCTC
GCACAATGTGCCTTCGGAAGCCTTTCTGCATGAATGCGATCGTCAAGGATTGCTCGTTATCGACGAGGCTTTTGACGGAT
GGCGTGATGCCAAGAACAAACATGACTATTCCGTGCTTTTTGACAAGTGGTGGAAACGTGATATTGAAGCGATGGTGCTT
CGCGACCGCAACCACCCCTCCGTATTCTGCTGGAGCATCGGCAATGAGGTGATAGAGCGCAAGAAACTGGAAGTAATCAC
TACGGCAAAGAGACTTGCCGAACACGTACATCGGTTGGATCCTTCACGTCCGGTCACTTCTGCACTAACTACTTGGGACA
AAGACTGGGAGATATTCGACCCGTTGGCAGCCGTACATGACATTGTAGGCTATAATTACCAGTTGCATCGTGCAGAATCT
GACCACGAACGTGTCCCCTCCCGCATCATCATGCAGACGGAATCCTATCCACGCGATGCTTTCCGCAATTGGGATTTGGT
AAACAAGCATCCCTATATCATCGGCGACTTTGTATGGACGGCATTGGATTATCTGGGAGAATCGAGCATCGGGCGTGCCT
ATTACAAAGGTAGGGAGAAAGACGGTTTCCATACCGGGCAGCTCTATCCTTGGCATGGTGCCTATTGTGGTGACATCGAC
CTGACCGGGCTGCGAAAACCCATATCCCATTATCGTGACATGCTCTACAACCCCGATAAGAAACTCTATATGGCCGTACG
TGAACCCAATGGTTACCGGGGAGAAATAAAAGTAACTGATTGGGGTGTATGGCCTACCAGCGAAAGCTGGACTTGGAGCG
GGCATGAAGGAAAACCGATAGAAGTAGAGGTCTGCTCCCGCTATCCCCGTGTGCGGCTTTTCTTGAACGATTCACTTGTG
GACGAACGCCCGGCAGGCTATGTACAACAGTTCAAGGCTGTCTTTACATTGCCCTATCAGGCAGGTACACTGCGCGCGGT
AGGTGTGGATGAGAACGGTATAGAGCAGGAAACCGTAGTGTTAAAGACTTCCGGAACTCCTGTTTCTCTACGCCTTACCA
CTCAAGATCAAACCATAAAGGCTGACGGACAAGATTTGGCTTTTGTTATCATTGAGGTCGTGGACAAAAATGGAAACGTA
GTACCTGATGCTGCTAATGAAGTGGAATTTTCCGTTCGTGGAACCGGTATGCTGGAGGCTACGGGCAGTGCGGATTTGAA
AGACGAAAAATCATATACGGCTCCCATACGTAAGGTCTGGAAAGGTCGTGCCATCGCTGTAATCAGAAGTACGAAACAAA
ACGGTAAAGTCAAACTGAAGGTGGCGTCAAAAGGACTTTCTCCGGCTTCAGTAATAATTAAAACTAAAAGATAA

Upstream 100 bases:

>100_bases
CGGAACAATCCATCTCCATAGGCTTTGGAAAAAAGTTCCTCAATACAGTGGTAAAGCCCCATTCATTCAATACCTATTGT
ATAAATCATAAGTAATTCTC

Downstream 100 bases:

>100_bases
ATAATATGAAAATAGGATTCCTAACAATTTTACTCTTCCTTGTCGTACAGACGGCATGGGGACAGTTCCGTGTGCATTCA
GGTATGACGGTTACAGTGAA

Product: beta-glycosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 797; Mature: 797

Protein sequence:

>797_residues
MKKFISISFFTWLFVMFSYPLFVEAAMPERKQLFDDNWRFKLGDTPDAPSLAYNDVGWRSLDLPHDWSVEADFDAEVPAG
NDGGYLPTGIGWYRKKFHVDKVMEGKELRLYFEGVYMNSEVFVNGERAGGHPYGYSSYFCDITPYLHFGQENIVAVRVDN
SQQKNCRWYSGSGIYRHVWLLTTEAIYIDDWSVYIRPTQKEGSDDWNLDIAMRYIDNARTGTKPEIKHTIYDEAGRVLVT
SASGHTKQSLSVSHPKLWSPDTPNLYKVCTQLIVGGKVVDEVTNMTGFRNITFDSQKGLFVNGKPILLNGGCVHHDNGIL
GACSFDAAEARKVRLLKEAGFNAVRTSHNVPSEAFLHECDRQGLLVIDEAFDGWRDAKNKHDYSVLFDKWWKRDIEAMVL
RDRNHPSVFCWSIGNEVIERKKLEVITTAKRLAEHVHRLDPSRPVTSALTTWDKDWEIFDPLAAVHDIVGYNYQLHRAES
DHERVPSRIIMQTESYPRDAFRNWDLVNKHPYIIGDFVWTALDYLGESSIGRAYYKGREKDGFHTGQLYPWHGAYCGDID
LTGLRKPISHYRDMLYNPDKKLYMAVREPNGYRGEIKVTDWGVWPTSESWTWSGHEGKPIEVEVCSRYPRVRLFLNDSLV
DERPAGYVQQFKAVFTLPYQAGTLRAVGVDENGIEQETVVLKTSGTPVSLRLTTQDQTIKADGQDLAFVIIEVVDKNGNV
VPDAANEVEFSVRGTGMLEATGSADLKDEKSYTAPIRKVWKGRAIAVIRSTKQNGKVKLKVASKGLSPASVIIKTKR

Sequences:

>Translated_797_residues
MKKFISISFFTWLFVMFSYPLFVEAAMPERKQLFDDNWRFKLGDTPDAPSLAYNDVGWRSLDLPHDWSVEADFDAEVPAG
NDGGYLPTGIGWYRKKFHVDKVMEGKELRLYFEGVYMNSEVFVNGERAGGHPYGYSSYFCDITPYLHFGQENIVAVRVDN
SQQKNCRWYSGSGIYRHVWLLTTEAIYIDDWSVYIRPTQKEGSDDWNLDIAMRYIDNARTGTKPEIKHTIYDEAGRVLVT
SASGHTKQSLSVSHPKLWSPDTPNLYKVCTQLIVGGKVVDEVTNMTGFRNITFDSQKGLFVNGKPILLNGGCVHHDNGIL
GACSFDAAEARKVRLLKEAGFNAVRTSHNVPSEAFLHECDRQGLLVIDEAFDGWRDAKNKHDYSVLFDKWWKRDIEAMVL
RDRNHPSVFCWSIGNEVIERKKLEVITTAKRLAEHVHRLDPSRPVTSALTTWDKDWEIFDPLAAVHDIVGYNYQLHRAES
DHERVPSRIIMQTESYPRDAFRNWDLVNKHPYIIGDFVWTALDYLGESSIGRAYYKGREKDGFHTGQLYPWHGAYCGDID
LTGLRKPISHYRDMLYNPDKKLYMAVREPNGYRGEIKVTDWGVWPTSESWTWSGHEGKPIEVEVCSRYPRVRLFLNDSLV
DERPAGYVQQFKAVFTLPYQAGTLRAVGVDENGIEQETVVLKTSGTPVSLRLTTQDQTIKADGQDLAFVIIEVVDKNGNV
VPDAANEVEFSVRGTGMLEATGSADLKDEKSYTAPIRKVWKGRAIAVIRSTKQNGKVKLKVASKGLSPASVIIKTKR
>Mature_797_residues
MKKFISISFFTWLFVMFSYPLFVEAAMPERKQLFDDNWRFKLGDTPDAPSLAYNDVGWRSLDLPHDWSVEADFDAEVPAG
NDGGYLPTGIGWYRKKFHVDKVMEGKELRLYFEGVYMNSEVFVNGERAGGHPYGYSSYFCDITPYLHFGQENIVAVRVDN
SQQKNCRWYSGSGIYRHVWLLTTEAIYIDDWSVYIRPTQKEGSDDWNLDIAMRYIDNARTGTKPEIKHTIYDEAGRVLVT
SASGHTKQSLSVSHPKLWSPDTPNLYKVCTQLIVGGKVVDEVTNMTGFRNITFDSQKGLFVNGKPILLNGGCVHHDNGIL
GACSFDAAEARKVRLLKEAGFNAVRTSHNVPSEAFLHECDRQGLLVIDEAFDGWRDAKNKHDYSVLFDKWWKRDIEAMVL
RDRNHPSVFCWSIGNEVIERKKLEVITTAKRLAEHVHRLDPSRPVTSALTTWDKDWEIFDPLAAVHDIVGYNYQLHRAES
DHERVPSRIIMQTESYPRDAFRNWDLVNKHPYIIGDFVWTALDYLGESSIGRAYYKGREKDGFHTGQLYPWHGAYCGDID
LTGLRKPISHYRDMLYNPDKKLYMAVREPNGYRGEIKVTDWGVWPTSESWTWSGHEGKPIEVEVCSRYPRVRLFLNDSLV
DERPAGYVQQFKAVFTLPYQAGTLRAVGVDENGIEQETVVLKTSGTPVSLRLTTQDQTIKADGQDLAFVIIEVVDKNGNV
VPDAANEVEFSVRGTGMLEATGSADLKDEKSYTAPIRKVWKGRAIAVIRSTKQNGKVKLKVASKGLSPASVIIKTKR

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=441, Percent_Identity=26.0770975056689, Blast_Score=100, Evalue=7e-21,
Organism=Escherichia coli, GI48994920, Length=603, Percent_Identity=25.5389718076285, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1787903, Length=463, Percent_Identity=25.7019438444924, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1786539, Length=455, Percent_Identity=27.2527472527473, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17510825, Length=456, Percent_Identity=28.9473684210526, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922582, Length=537, Percent_Identity=25.6983240223464, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI62471735, Length=537, Percent_Identity=25.6983240223464, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24655438, Length=537, Percent_Identity=25.6983240223464, Blast_Score=134, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24651745, Length=573, Percent_Identity=25.6544502617801, Blast_Score=125, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 90669; Mature: 90669

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFISISFFTWLFVMFSYPLFVEAAMPERKQLFDDNWRFKLGDTPDAPSLAYNDVGWRS
CCCEEEHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCEEEECCCCCCCCCEECCCCCEE
LDLPHDWSVEADFDAEVPAGNDGGYLPTGIGWYRKKFHVDKVMEGKELRLYFEGVYMNSE
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCE
VFVNGERAGGHPYGYSSYFCDITPYLHFGQENIVAVRVDNSQQKNCRWYSGSGIYRHVWL
EEEECCCCCCCCCCCCCEEEEECCEEECCCCCEEEEEECCCCCCCCEEECCCCCEEEEEE
LTTEAIYIDDWSVYIRPTQKEGSDDWNLDIAMRYIDNARTGTKPEIKHTIYDEAGRVLVT
EEEEEEEEECEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCCEEEE
SASGHTKQSLSVSHPKLWSPDTPNLYKVCTQLIVGGKVVDEVTNMTGFRNITFDSQKGLF
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCEE
VNGKPILLNGGCVHHDNGILGACSFDAAEARKVRLLKEAGFNAVRTSHNVPSEAFLHECD
ECCCEEEEECCEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHCC
RQGLLVIDEAFDGWRDAKNKHDYSVLFDKWWKRDIEAMVLRDRNHPSVFCWSIGNEVIER
CCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEECCCHHHHHH
KKLEVITTAKRLAEHVHRLDPSRPVTSALTTWDKDWEIFDPLAAVHDIVGYNYQLHRAES
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCC
DHERVPSRIIMQTESYPRDAFRNWDLVNKHPYIIGDFVWTALDYLGESSIGRAYYKGREK
HHHHCCCEEEEECCCCCHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCHHHHCCCCC
DGFHTGQLYPWHGAYCGDIDLTGLRKPISHYRDMLYNPDKKLYMAVREPNGYRGEIKVTD
CCCCCCCEECCCCCEECCEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEE
WGVWPTSESWTWSGHEGKPIEVEVCSRYPRVRLFLNDSLVDERPAGYVQQFKAVFTLPYQ
ECCCCCCCCEEECCCCCCEEEEHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHEECCCC
AGTLRAVGVDENGIEQETVVLKTSGTPVSLRLTTQDQTIKADGQDLAFVIIEVVDKNGNV
CCEEEEEECCCCCCCCEEEEEEECCCEEEEEEECCCCEEECCCCCEEEEEEEEECCCCCC
VPDAANEVEFSVRGTGMLEATGSADLKDEKSYTAPIRKVWKGRAIAVIRSTKQNGKVKLK
CCCCCCCEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEE
VASKGLSPASVIIKTKR
EECCCCCCEEEEEEECC
>Mature Secondary Structure
MKKFISISFFTWLFVMFSYPLFVEAAMPERKQLFDDNWRFKLGDTPDAPSLAYNDVGWRS
CCCEEEHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCEEEECCCCCCCCCEECCCCCEE
LDLPHDWSVEADFDAEVPAGNDGGYLPTGIGWYRKKFHVDKVMEGKELRLYFEGVYMNSE
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCE
VFVNGERAGGHPYGYSSYFCDITPYLHFGQENIVAVRVDNSQQKNCRWYSGSGIYRHVWL
EEEECCCCCCCCCCCCCEEEEECCEEECCCCCEEEEEECCCCCCCCEEECCCCCEEEEEE
LTTEAIYIDDWSVYIRPTQKEGSDDWNLDIAMRYIDNARTGTKPEIKHTIYDEAGRVLVT
EEEEEEEEECEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCCEEEE
SASGHTKQSLSVSHPKLWSPDTPNLYKVCTQLIVGGKVVDEVTNMTGFRNITFDSQKGLF
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCEE
VNGKPILLNGGCVHHDNGILGACSFDAAEARKVRLLKEAGFNAVRTSHNVPSEAFLHECD
ECCCEEEEECCEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHCC
RQGLLVIDEAFDGWRDAKNKHDYSVLFDKWWKRDIEAMVLRDRNHPSVFCWSIGNEVIER
CCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEECCCHHHHHH
KKLEVITTAKRLAEHVHRLDPSRPVTSALTTWDKDWEIFDPLAAVHDIVGYNYQLHRAES
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCC
DHERVPSRIIMQTESYPRDAFRNWDLVNKHPYIIGDFVWTALDYLGESSIGRAYYKGREK
HHHHCCCEEEEECCCCCHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCHHHHCCCCC
DGFHTGQLYPWHGAYCGDIDLTGLRKPISHYRDMLYNPDKKLYMAVREPNGYRGEIKVTD
CCCCCCCEECCCCCEECCEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEE
WGVWPTSESWTWSGHEGKPIEVEVCSRYPRVRLFLNDSLVDERPAGYVQQFKAVFTLPYQ
ECCCCCCCCEEECCCCCCEEEEHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHEECCCC
AGTLRAVGVDENGIEQETVVLKTSGTPVSLRLTTQDQTIKADGQDLAFVIIEVVDKNGNV
CCEEEEEECCCCCCCCEEEEEEECCCEEEEEEECCCCEEECCCCCEEEEEEEEECCCCCC
VPDAANEVEFSVRGTGMLEATGSADLKDEKSYTAPIRKVWKGRAIAVIRSTKQNGKVKLK
CCCCCCCEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEE
VASKGLSPASVIIKTKR
EECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA