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The map label for this gene is ygcM [C]

Identifier: 150004623

GI number: 150004623

Start: 2695644

End: 2695976

Strand: Direct

Name: ygcM [C]

Synonym: BVU_2082

Alternate gene names: 150004623

Gene position: 2695644-2695976 (Clockwise)

Preceding gene: 150004617

Following gene: 150004624

Centisome position: 52.21

GC content: 40.24

Gene sequence:

>333_bases
ATGTATACAGTAATTAAACGAATGGAAATTTCAGCTTCCCATAGCTTGAAGCTGTCGTATAGAAGTAAATGTGAGAATTT
GCACGGTCACAATTGGATTATTACCGTTTATTGTCGTTCCGAGGTTTTGAACGAGGATGGTATGGTGGTGGATTTCACGC
ATATCAAGGAAACGGTGATGGGGCGTTTGGATCACCGGAATTTGAATGAGGTTGTTTCTTTTAATCCTACAGCTGAAAAT
ATTGCACGCTGGGTATGTGAGCAAATTCCCACTTGTTTCAAGGTTGAAGTTCGCGAGTCAGAAGGAAATACCGTTATCTA
TGAGAAGGATTAA

Upstream 100 bases:

>100_bases
AGTGTCCTTTTTATATTATTCTTTTTTATGGTGTATCTTTGCGTCCTGTAGCACCGAGGTGACATTAGGTGTGAGTTCTT
TCTTATTATAATTAAATGCT

Downstream 100 bases:

>100_bases
TGAAATATTTTACAGCTTACAGGGGGAGGGGTATCATACTGGAATACCTGCCGTTTTTGTACGTTTTTCCGGGTGCAATT
TGAGATGTCCTTTTTGCGAT

Product: 6-pyruvoyl-tetrahydropterin synthase

Products: 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine; Triphosphate. [C]

Alternate protein names: Queuosine Biosynthesis Protein QueD; 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl Tetrahydrobiopterin Synthase Family Protein; Queuosine Biosynthesis Protein

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MYTVIKRMEISASHSLKLSYRSKCENLHGHNWIITVYCRSEVLNEDGMVVDFTHIKETVMGRLDHRNLNEVVSFNPTAEN
IARWVCEQIPTCFKVEVRESEGNTVIYEKD

Sequences:

>Translated_110_residues
MYTVIKRMEISASHSLKLSYRSKCENLHGHNWIITVYCRSEVLNEDGMVVDFTHIKETVMGRLDHRNLNEVVSFNPTAEN
IARWVCEQIPTCFKVEVRESEGNTVIYEKD
>Mature_110_residues
MYTVIKRMEISASHSLKLSYRSKCENLHGHNWIITVYCRSEVLNEDGMVVDFTHIKETVMGRLDHRNLNEVVSFNPTAEN
IARWVCEQIPTCFKVEVRESEGNTVIYEKD

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12 [C]

Molecular weight: Translated: 12835; Mature: 12835

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTVIKRMEISASHSLKLSYRSKCENLHGHNWIITVYCRSEVLNEDGMVVDFTHIKETVM
CCCEEEEEECCCCCEEEEEHHHHCCCCCCCCEEEEEEEECCCCCCCCCEEEHHHHHHHHH
GRLDHRNLNEVVSFNPTAENIARWVCEQIPTCFKVEVRESEGNTVIYEKD
HHCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECC
>Mature Secondary Structure
MYTVIKRMEISASHSLKLSYRSKCENLHGHNWIITVYCRSEVLNEDGMVVDFTHIKETVM
CCCEEEEEECCCCCEEEEEHHHHCCCCCCCCEEEEEEEECCCCCCCCCEEEHHHHHHHHH
GRLDHRNLNEVVSFNPTAENIARWVCEQIPTCFKVEVRESEGNTVIYEKD
HHCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate [C]

Specific reaction: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate = 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine + Triphosphate. [C]

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA