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The map label for this gene is pfkA2 [H]

Identifier: 150004486

GI number: 150004486

Start: 2517126

End: 2518106

Strand: Reverse

Name: pfkA2 [H]

Synonym: BVU_1935

Alternate gene names: 150004486

Gene position: 2518106-2517126 (Counterclockwise)

Preceding gene: 150004487

Following gene: 150004483

Centisome position: 48.77

GC content: 44.75

Gene sequence:

>981_bases
ATGGGAACGATTAAAAGTATTGGTATTTTAACCTCTGGAGGAGATGCTCCGGGAATGAATGCTGCCATTCGTGCAGTCAC
ACGTTCTGCCATCTATAACGGCTTGAAAGTATATGGCATTTATAGAGGTTACAAAGGATTGGTAACAGATGAAATTCAGG
AATTCAAAAGCCAAAATGTTAGCAACATCATACAAATGGGAGGTACCATTCTGAAAACCGCCCGTTGCAAAGAATTCACG
ACACCCGAAGGACGGGCACAAGCTTACGAAAATATGAAAAAGCACGAGATTGATGCTTTGGTCATTATCGGCGGTGACGG
TTCGTTGACCGGTGCACGTATCTTTGCTCAGGAATTTGATGTGCCTTGTATCGGTCTGCCCGGAACGATTGACAACGACC
TGTATGGAACCGACACCACCATCGGCTATGATACAGCTTTGAACACAATTTTGGATGCAGTAGATAAAATCCGTGATACG
GCAACTTCTCACGAACGCTTATTTTTTGTTGAAGTGATGGGACGTGATGCAGGTTTCCTGGCATTGAACGGCGCTATTGC
CGCAGGTGCAGAAGCAGCTATTATTCCGGAATTCAGCACAGAAGTTGACCAATTGGAAGAATTCATCGAACATGGTTTCC
GCAAATCCAAGAGCAGTAGCATCGTATTAGTAGCCGAAAGCGAACTGACCGGGGGTGCAATGCACTATGCCGAACGTGTG
AAGAACGAATATCCGCAATACGATGTGCGTGTAACGATATTAGGACATTTACAACGTGGCGGACGCCCCACAGCGCATGA
CCGAATCATTGCCAGCCGTATGGGAGTGGCCAGCATCCAAGCACTATTGGAAGGACAACGTAATGTAATGATTGGTATTG
ATGATGATAAGATTGTTTATGTACCTTTCGCTAAAGCTATAAAGAACGACAAGCCTATAGACAGAGAATTGGTGAATGTA
CTTCACGAACTTTCTATCTAA

Upstream 100 bases:

>100_bases
AATCTTAAACGAAAAATAGACCAATAAAAAATAGAAAATTATCGTTGTCCAACTGATAATTTGTTAACTTTGCGACATAA
AAATATTAAAATGGGTATTT

Downstream 100 bases:

>100_bases
TTTCATCCACCACAGACAATAAAAAAAACGCAGATTTAATGAATAATCATAATCTGCGTTTTTTGTTTTGCCTGTTGGTA
GCAAAACCTTATTTCAAGGT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MGTIKSIGILTSGGDAPGMNAAIRAVTRSAIYNGLKVYGIYRGYKGLVTDEIQEFKSQNVSNIIQMGGTILKTARCKEFT
TPEGRAQAYENMKKHEIDALVIIGGDGSLTGARIFAQEFDVPCIGLPGTIDNDLYGTDTTIGYDTALNTILDAVDKIRDT
ATSHERLFFVEVMGRDAGFLALNGAIAAGAEAAIIPEFSTEVDQLEEFIEHGFRKSKSSSIVLVAESELTGGAMHYAERV
KNEYPQYDVRVTILGHLQRGGRPTAHDRIIASRMGVASIQALLEGQRNVMIGIDDDKIVYVPFAKAIKNDKPIDRELVNV
LHELSI

Sequences:

>Translated_326_residues
MGTIKSIGILTSGGDAPGMNAAIRAVTRSAIYNGLKVYGIYRGYKGLVTDEIQEFKSQNVSNIIQMGGTILKTARCKEFT
TPEGRAQAYENMKKHEIDALVIIGGDGSLTGARIFAQEFDVPCIGLPGTIDNDLYGTDTTIGYDTALNTILDAVDKIRDT
ATSHERLFFVEVMGRDAGFLALNGAIAAGAEAAIIPEFSTEVDQLEEFIEHGFRKSKSSSIVLVAESELTGGAMHYAERV
KNEYPQYDVRVTILGHLQRGGRPTAHDRIIASRMGVASIQALLEGQRNVMIGIDDDKIVYVPFAKAIKNDKPIDRELVNV
LHELSI
>Mature_325_residues
GTIKSIGILTSGGDAPGMNAAIRAVTRSAIYNGLKVYGIYRGYKGLVTDEIQEFKSQNVSNIIQMGGTILKTARCKEFTT
PEGRAQAYENMKKHEIDALVIIGGDGSLTGARIFAQEFDVPCIGLPGTIDNDLYGTDTTIGYDTALNTILDAVDKIRDTA
TSHERLFFVEVMGRDAGFLALNGAIAAGAEAAIIPEFSTEVDQLEEFIEHGFRKSKSSSIVLVAESELTGGAMHYAERVK
NEYPQYDVRVTILGHLQRGGRPTAHDRIIASRMGVASIQALLEGQRNVMIGIDDDKIVYVPFAKAIKNDKPIDRELVNVL
HELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=351, Percent_Identity=43.019943019943, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI11321601, Length=351, Percent_Identity=42.4501424501425, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI266453768, Length=352, Percent_Identity=40.3409090909091, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI266453748, Length=352, Percent_Identity=40.3409090909091, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI4505749, Length=352, Percent_Identity=40.3409090909091, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI266453619, Length=352, Percent_Identity=40.3409090909091, Blast_Score=248, Evalue=5e-66,
Organism=Escherichia coli, GI1790350, Length=323, Percent_Identity=47.3684210526316, Blast_Score=273, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI25147584, Length=353, Percent_Identity=41.0764872521246, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=38.5113268608414, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI72003601, Length=332, Percent_Identity=30.421686746988, Blast_Score=151, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321679, Length=349, Percent_Identity=39.8280802292264, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=37.6811594202899, Blast_Score=219, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24652337, Length=348, Percent_Identity=41.9540229885057, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17647809, Length=347, Percent_Identity=41.7867435158501, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=42.3631123919308, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35427; Mature: 35296

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTIKSIGILTSGGDAPGMNAAIRAVTRSAIYNGLKVYGIYRGYKGLVTDEIQEFKSQNV
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCH
SNIIQMGGTILKTARCKEFTTPEGRAQAYENMKKHEIDALVIIGGDGSLTGARIFAQEFD
HHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCC
VPCIGLPGTIDNDLYGTDTTIGYDTALNTILDAVDKIRDTATSHERLFFVEVMGRDAGFL
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEE
ALNGAIAAGAEAAIIPEFSTEVDQLEEFIEHGFRKSKSSSIVLVAESELTGGAMHYAERV
EECCCHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHH
KNEYPQYDVRVTILGHLQRGGRPTAHDRIIASRMGVASIQALLEGQRNVMIGIDDDKIVY
HHCCCCCEEEEEEEEEHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEE
VPFAKAIKNDKPIDRELVNVLHELSI
EECHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTIKSIGILTSGGDAPGMNAAIRAVTRSAIYNGLKVYGIYRGYKGLVTDEIQEFKSQNV
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCH
SNIIQMGGTILKTARCKEFTTPEGRAQAYENMKKHEIDALVIIGGDGSLTGARIFAQEFD
HHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCC
VPCIGLPGTIDNDLYGTDTTIGYDTALNTILDAVDKIRDTATSHERLFFVEVMGRDAGFL
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEE
ALNGAIAAGAEAAIIPEFSTEVDQLEEFIEHGFRKSKSSSIVLVAESELTGGAMHYAERV
EECCCHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHH
KNEYPQYDVRVTILGHLQRGGRPTAHDRIIASRMGVASIQALLEGQRNVMIGIDDDKIVY
HHCCCCCEEEEEEEEEHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEE
VPFAKAIKNDKPIDRELVNVLHELSI
EECHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]