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The map label for this gene is metF [H]

Identifier: 150004384

GI number: 150004384

Start: 2376389

End: 2377342

Strand: Reverse

Name: metF [H]

Synonym: BVU_1830

Alternate gene names: 150004384

Gene position: 2377342-2376389 (Counterclockwise)

Preceding gene: 150004385

Following gene: 150004383

Centisome position: 46.04

GC content: 43.71

Gene sequence:

>954_bases
ATGAGAGTAGTTGATTTAATAAAAAGCACGGACAATACGGCTTTTTCTTTTGAAATTCTCCCTCCGCTGAAAGGAACGGG
TATTGAAAAACTGTATAAAACGGTTGATACGCTACGCGAATTCGACCCTAAATATATCAATATTACAACCCACCGCAGCG
AATATGTGTACAAGGAGCTGGGAAACGGGCTGTACCAACGTACACGCCAGCGCCGCCGTCCCGGAACAGTTGCTGTTGCC
GCCGCTTTGCAGAACAAATACAATATAACGACAGTGCCACATATCCTGTGCAGCGGTTTTTCCCGCGAAGATACAGAATA
TGTATTGTTAGATCTACAATTCCTGGGCATCACCGACTTGTTGGTACTGCGTGGTGACAAGGCAAAGCACGAATCAGCTT
TCGTACCCGAAAACAACGGTTACAACCACGCCATAGAACTGGAAGAGCAGATCAATGATTTCAATAAAGGCGTATTTGTA
GACGGTTCCCCCATCAAGGTAACAGGAACTCCGTTCAGTTATGGAGTAGCCTGCTATCCTGAAAAACACGAGGAATCACC
CAATATGGAACAAGATATTTATTGGCTGAAGAAAAAGATTGAAGCAGGAGCTGAATATGCTGTGACACAGATGTTCTATG
ACAACCGCAAATATTTCGAATTTGTAGAAAGGGTAAGAAAAGAAGGAATCAATGTACCTATTATTCCGGGTATCAAGCCT
TTCCGCAAATTATCCCAGCTGAACATGATACCCAAGACGTTCAAGATAGACTTGCCGCAAGAACTGGCAGGCGAAGCGAT
GAAATGTCAAACAGATGAAGAAGCGGAAAGTCTTGGCATAGAATGGTGCATACACCAGTGCCGTGAATTAATAGCCTGTG
GGGTTCCCAGCATTCATTTCTATACCGTAAGTGCCGTGAACAGTGTAAAGGAAGTTGCAAAAGCAATTTATTAA

Upstream 100 bases:

>100_bases
AATAAATCGCTTTTTCATCCCCATAAACTACGATATTTTTCTTATTTTTGCCCAAATATGTAATTCCGCCCGATAAGGCA
TAAGAGAACGAGACAGAACG

Downstream 100 bases:

>100_bases
ACAGTAAATAAATGACTATAAAAAGCAATGTTATTTAAAGATGTAATAGGACAAGAGGAAGCAAAACAGCGGCTCATAGC
CGAGGTAAAAGAAGGAAGAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRVVDLIKSTDNTAFSFEILPPLKGTGIEKLYKTVDTLREFDPKYINITTHRSEYVYKELGNGLYQRTRQRRRPGTVAVA
AALQNKYNITTVPHILCSGFSREDTEYVLLDLQFLGITDLLVLRGDKAKHESAFVPENNGYNHAIELEEQINDFNKGVFV
DGSPIKVTGTPFSYGVACYPEKHEESPNMEQDIYWLKKKIEAGAEYAVTQMFYDNRKYFEFVERVRKEGINVPIIPGIKP
FRKLSQLNMIPKTFKIDLPQELAGEAMKCQTDEEAESLGIEWCIHQCRELIACGVPSIHFYTVSAVNSVKEVAKAIY

Sequences:

>Translated_317_residues
MRVVDLIKSTDNTAFSFEILPPLKGTGIEKLYKTVDTLREFDPKYINITTHRSEYVYKELGNGLYQRTRQRRRPGTVAVA
AALQNKYNITTVPHILCSGFSREDTEYVLLDLQFLGITDLLVLRGDKAKHESAFVPENNGYNHAIELEEQINDFNKGVFV
DGSPIKVTGTPFSYGVACYPEKHEESPNMEQDIYWLKKKIEAGAEYAVTQMFYDNRKYFEFVERVRKEGINVPIIPGIKP
FRKLSQLNMIPKTFKIDLPQELAGEAMKCQTDEEAESLGIEWCIHQCRELIACGVPSIHFYTVSAVNSVKEVAKAIY
>Mature_317_residues
MRVVDLIKSTDNTAFSFEILPPLKGTGIEKLYKTVDTLREFDPKYINITTHRSEYVYKELGNGLYQRTRQRRRPGTVAVA
AALQNKYNITTVPHILCSGFSREDTEYVLLDLQFLGITDLLVLRGDKAKHESAFVPENNGYNHAIELEEQINDFNKGVFV
DGSPIKVTGTPFSYGVACYPEKHEESPNMEQDIYWLKKKIEAGAEYAVTQMFYDNRKYFEFVERVRKEGINVPIIPGIKP
FRKLSQLNMIPKTFKIDLPQELAGEAMKCQTDEEAESLGIEWCIHQCRELIACGVPSIHFYTVSAVNSVKEVAKAIY

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=306, Percent_Identity=29.0849673202614, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790377, Length=297, Percent_Identity=25.5892255892256, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25147441, Length=304, Percent_Identity=27.3026315789474, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25147436, Length=304, Percent_Identity=27.3026315789474, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321313, Length=302, Percent_Identity=25.8278145695364, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325234, Length=334, Percent_Identity=25.748502994012, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 36180; Mature: 36180

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVDLIKSTDNTAFSFEILPPLKGTGIEKLYKTVDTLREFDPKYINITTHRSEYVYKEL
CCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
GNGLYQRTRQRRRPGTVAVAAALQNKYNITTVPHILCSGFSREDTEYVLLDLQFLGITDL
CCHHHHHHHHHCCCCCEEHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEEHHCHHEE
LVLRGDKAKHESAFVPENNGYNHAIELEEQINDFNKGVFVDGSPIKVTGTPFSYGVACYP
EEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEECCCEEEEECCCCCCCEEECC
EKHEESPNMEQDIYWLKKKIEAGAEYAVTQMFYDNRKYFEFVERVRKEGINVPIIPGIKP
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHH
FRKLSQLNMIPKTFKIDLPQELAGEAMKCQTDEEAESLGIEWCIHQCRELIACGVPSIHF
HHHHHHHCCCCCCEEECCCHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
YTVSAVNSVKEVAKAIY
EEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVVDLIKSTDNTAFSFEILPPLKGTGIEKLYKTVDTLREFDPKYINITTHRSEYVYKEL
CCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
GNGLYQRTRQRRRPGTVAVAAALQNKYNITTVPHILCSGFSREDTEYVLLDLQFLGITDL
CCHHHHHHHHHCCCCCEEHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEEHHCHHEE
LVLRGDKAKHESAFVPENNGYNHAIELEEQINDFNKGVFVDGSPIKVTGTPFSYGVACYP
EEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEECCCEEEEECCCCCCCEEECC
EKHEESPNMEQDIYWLKKKIEAGAEYAVTQMFYDNRKYFEFVERVRKEGINVPIIPGIKP
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHH
FRKLSQLNMIPKTFKIDLPQELAGEAMKCQTDEEAESLGIEWCIHQCRELIACGVPSIHF
HHHHHHHCCCCCCEEECCCHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
YTVSAVNSVKEVAKAIY
EEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]