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The map label for this gene is cls2 [H]

Identifier: 150004385

GI number: 150004385

Start: 2377698

End: 2378993

Strand: Reverse

Name: cls2 [H]

Synonym: BVU_1831

Alternate gene names: 150004385

Gene position: 2378993-2377698 (Counterclockwise)

Preceding gene: 150004387

Following gene: 150004384

Centisome position: 46.08

GC content: 42.05

Gene sequence:

>1296_bases
ATGAAGTTTGATATAAAAAAACATATTTATTTAATACTTTTATTCATCTTATCTTTCACTGTAGAGACAGCTCAGTCACA
AGAAGTTACCAACATCCATATAGCAGCGACAGATACGGTGCCAACCTATACCAGCGACTCTGCCGTTATCAATTTCTTAA
AGGATGCCGGCATTCCTATCACCCAAAACAGCAAACTGAAATTATTAAAAAGCGGTCGGGCCAAATTCATCGATCTCTTT
GAAGAAATACGGCATGCCAAACATCATATCCACTTAGAATACTTCAATTTTCGTAACGATTCCATAGCGAACGCACTATT
TGATTTATTGGGAGAAAAAGTGAAAGAAGGAGTGGAAGTCAGAGCTTTGTTTGATGCCTTCGGCAATTTGTCAAACAACA
AGCCATTGAAAAAGAAACACATACAAGCAATCCGTGACAAAGGAATCGAAATTGTCAAGTTCGACCCTTTCAAATTCCCC
TATATCAATCATGCCCTGCACAGAGACCATCGCAAGATTGTAGTCATAGACGGAAAAATAGGATATACCGGTGGCATGAA
CATAGCCAATTACTATATAAAGGGTCTGCCGGAAATAGGCGACTGGCGCGACATGCATATCCGTATAGAGGGAAATGCCG
TAAACGAATTGCAGGACATCTTCCTGGCCATGTGGAACAAAAGCACTAAACAACACGTAAGCGGAAGCCAATACTATCCC
CTACGAAACGATTCTACTTTCAAGGGAAATAAGAATGTAGCCATTGTAGACCGCATTCCGAAAAAAGAACCCAGACTGAT
GAGGCAGACTTATATCAAGTCCATTGATGCCGCACAGGATAAAATACAAATAGTGAATCCCTATTTCACTCCCATTCCAA
GCATTAAAAAAGCCATAAAACGTGCGTTGAAGCGAGGCGTTGAAGTAGAAATCATGATACCGGGCAAATCGGATATTCCT
TTTACCCCCGATGCCGCGTTCTATACAGCAAACAAGCTGCGTAAGAAAGGAGCAAAGATTTATGTGTACAATGGCGGGTT
CCACCACTCTAAGATCATGATGGTGGACAGCCTGTTCTGCACTGTGGGAAGTACGAATCTGAACAGCCGCAGCCTACGCT
ATGACTATGAGGTCAACGCTTTCATTTTTGACAAAGAAACCACACATGAACTGAACACCATGTTCGAGCATGACAAGCTG
GACAGTACCCTGTTTACCAAGGAAGAATATAAAAAACGCTCCGGATGGAAACGCTTCGTCGGTTGGTTCGCCAATCTGTT
CACTCCATTCCTATAA

Upstream 100 bases:

>100_bases
TGGCCACACTGTTCTATTTCTCCCTTTTAAACATAAATAATACCGATGGCTTGTTCATTCCTAGAATAATAAATACCTTT
GCAGCCTTAATAAAAAAGCA

Downstream 100 bases:

>100_bases
ACCTCCGCTACTTCCCCAAACAAAGGTTTACAGATTATTTCATCTCCTGCAAGGACGGGGCTTATCCCATCCGCAAGTAT
TCTCCATGCAGTAAAGCTTG

Product: putative phospholipid biosynthesis related protein

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKFDIKKHIYLILLFILSFTVETAQSQEVTNIHIAATDTVPTYTSDSAVINFLKDAGIPITQNSKLKLLKSGRAKFIDLF
EEIRHAKHHIHLEYFNFRNDSIANALFDLLGEKVKEGVEVRALFDAFGNLSNNKPLKKKHIQAIRDKGIEIVKFDPFKFP
YINHALHRDHRKIVVIDGKIGYTGGMNIANYYIKGLPEIGDWRDMHIRIEGNAVNELQDIFLAMWNKSTKQHVSGSQYYP
LRNDSTFKGNKNVAIVDRIPKKEPRLMRQTYIKSIDAAQDKIQIVNPYFTPIPSIKKAIKRALKRGVEVEIMIPGKSDIP
FTPDAAFYTANKLRKKGAKIYVYNGGFHHSKIMMVDSLFCTVGSTNLNSRSLRYDYEVNAFIFDKETTHELNTMFEHDKL
DSTLFTKEEYKKRSGWKRFVGWFANLFTPFL

Sequences:

>Translated_431_residues
MKFDIKKHIYLILLFILSFTVETAQSQEVTNIHIAATDTVPTYTSDSAVINFLKDAGIPITQNSKLKLLKSGRAKFIDLF
EEIRHAKHHIHLEYFNFRNDSIANALFDLLGEKVKEGVEVRALFDAFGNLSNNKPLKKKHIQAIRDKGIEIVKFDPFKFP
YINHALHRDHRKIVVIDGKIGYTGGMNIANYYIKGLPEIGDWRDMHIRIEGNAVNELQDIFLAMWNKSTKQHVSGSQYYP
LRNDSTFKGNKNVAIVDRIPKKEPRLMRQTYIKSIDAAQDKIQIVNPYFTPIPSIKKAIKRALKRGVEVEIMIPGKSDIP
FTPDAAFYTANKLRKKGAKIYVYNGGFHHSKIMMVDSLFCTVGSTNLNSRSLRYDYEVNAFIFDKETTHELNTMFEHDKL
DSTLFTKEEYKKRSGWKRFVGWFANLFTPFL
>Mature_431_residues
MKFDIKKHIYLILLFILSFTVETAQSQEVTNIHIAATDTVPTYTSDSAVINFLKDAGIPITQNSKLKLLKSGRAKFIDLF
EEIRHAKHHIHLEYFNFRNDSIANALFDLLGEKVKEGVEVRALFDAFGNLSNNKPLKKKHIQAIRDKGIEIVKFDPFKFP
YINHALHRDHRKIVVIDGKIGYTGGMNIANYYIKGLPEIGDWRDMHIRIEGNAVNELQDIFLAMWNKSTKQHVSGSQYYP
LRNDSTFKGNKNVAIVDRIPKKEPRLMRQTYIKSIDAAQDKIQIVNPYFTPIPSIKKAIKRALKRGVEVEIMIPGKSDIP
FTPDAAFYTANKLRKKGAKIYVYNGGFHHSKIMMVDSLFCTVGSTNLNSRSLRYDYEVNAFIFDKETTHELNTMFEHDKL
DSTLFTKEEYKKRSGWKRFVGWFANLFTPFL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=383, Percent_Identity=26.6318537859008, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787007, Length=358, Percent_Identity=25.9776536312849, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 49708; Mature: 49708

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDIKKHIYLILLFILSFTVETAQSQEVTNIHIAATDTVPTYTSDSAVINFLKDAGIPI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCC
TQNSKLKLLKSGRAKFIDLFEEIRHAKHHIHLEYFNFRNDSIANALFDLLGEKVKEGVEV
CCCCCHHHHHCCCHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHH
RALFDAFGNLSNNKPLKKKHIQAIRDKGIEIVKFDPFKFPYINHALHRDHRKIVVIDGKI
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCEEEEEECCC
GYTGGMNIANYYIKGLPEIGDWRDMHIRIEGNAVNELQDIFLAMWNKSTKQHVSGSQYYP
CCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCCEEEC
LRNDSTFKGNKNVAIVDRIPKKEPRLMRQTYIKSIDAAQDKIQIVNPYFTPIPSIKKAIK
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHH
RALKRGVEVEIMIPGKSDIPFTPDAAFYTANKLRKKGAKIYVYNGGFHHSKIMMVDSLFC
HHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHHH
TVGSTNLNSRSLRYDYEVNAFIFDKETTHELNTMFEHDKLDSTLFTKEEYKKRSGWKRFV
CCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCHHHCHHHHHHHCCHHHHH
GWFANLFTPFL
HHHHHHHCCCC
>Mature Secondary Structure
MKFDIKKHIYLILLFILSFTVETAQSQEVTNIHIAATDTVPTYTSDSAVINFLKDAGIPI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCC
TQNSKLKLLKSGRAKFIDLFEEIRHAKHHIHLEYFNFRNDSIANALFDLLGEKVKEGVEV
CCCCCHHHHHCCCHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHH
RALFDAFGNLSNNKPLKKKHIQAIRDKGIEIVKFDPFKFPYINHALHRDHRKIVVIDGKI
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCEEEEEECCC
GYTGGMNIANYYIKGLPEIGDWRDMHIRIEGNAVNELQDIFLAMWNKSTKQHVSGSQYYP
CCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCCEEEC
LRNDSTFKGNKNVAIVDRIPKKEPRLMRQTYIKSIDAAQDKIQIVNPYFTPIPSIKKAIK
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHH
RALKRGVEVEIMIPGKSDIPFTPDAAFYTANKLRKKGAKIYVYNGGFHHSKIMMVDSLFC
HHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHHH
TVGSTNLNSRSLRYDYEVNAFIFDKETTHELNTMFEHDKLDSTLFTKEEYKKRSGWKRFV
CCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCHHHCHHHHHHHCCHHHHH
GWFANLFTPFL
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA