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The map label for this gene is mrdA [H]

Identifier: 150004379

GI number: 150004379

Start: 2369884

End: 2371734

Strand: Direct

Name: mrdA [H]

Synonym: BVU_1825

Alternate gene names: 150004379

Gene position: 2369884-2371734 (Clockwise)

Preceding gene: 150004378

Following gene: 150004380

Centisome position: 45.9

GC content: 46.52

Gene sequence:

>1851_bases
GTGGAAAGAGATTATAATCTGGAGAAGCGGAAATATGTAATCGGTGCATCGGTGATCGTTATTGTGCTGATTTACCTGAT
ACGTCTTTTTACCTTGCAGATTATGAGTGAGGATTATAAAAAGAACGCCGATAGCAACGCTTTTCTGAACAAGACACAAT
ACCCTAGCCGTGGGGTGATGTACGATCGGAACGGTAATCTGCTGGTGTATAACCAACCTGCCTATGACGTTACGATGGTG
ATGAAAGAGGTGCACAACCTTGATACGCTTGATTTATGTAAAACATTGAATATTACCCCCGACTATTTTAAAAAGCGTAT
CCGCGAGATAAAGGACAGACGTTCCAATCCGGGCTACTCTCCATATACTCATCAGGTATTTATGACCCAGCTTTCGGCAG
AAGAGTGCGGAGTCTTCCAAGAAAAACTGTTCAAGTTTCCCGGTTTCTATATTCAGCGGCGTACTATCCGCCAGTATAAT
TACAATGCGGCTGCTCATGTTCTGGGAGATATTGCAGAAGTGTCCAAGGGGGATATTGAAGCCGATGATTACTATGTACG
CGGTGACTTCATAGGGAAACAGGGGGTGGAACGCTCATACGAAAAACAGTTGAGGGGCGAGAAAGGAGTGGAGATATTAC
TGCGCGATGCCCGTGGACGTATTCAGGGACGGTATATGGACGGGAAATATGACAAGACTCCCGTTCCGGGGAAGAATCTG
AAACTAGGTATAGACATAGAGCTTCAGATGTTGGGAGAGCGTCTGTTGGAAGGGAAGATAGGCAGTATAGTGGCTATCGA
GCCATCCACAGGAGAAATACTTTGTATGGTATCTGCTCCCACTTTTGACCCAAGATTGATGGTGGGGCGCCAACGGGGTA
AAAATCATCTGGAACTGGCGCGTGACAGTTGGAAACCATTGCTGAACCGTTCTATTATGGGGCAATTCCCACCGGGATCT
ACTTTCAAGACTACTCAGGCGTTGACTTTCCTGCAAGAGGGGATTATTACTCCTCAGACAGCCTATTCTTGCTATCATGG
CTTTGTGTATGCCGGTCTTAGGGTGGGCTGCCACTCTCATGGGTCGCCCCTGCCTTTGGTGCCTGCCATTGCAACTTCCT
GTAACGGTTATTTCTGTTGGGGGTTGTTTCACATGATAGGGGCGAAGAAGAAGTATGGTTCTGTACAGACTGCCATGAAT
ACATGGCGTGATTATATGGTTTCTATGGGATTTGGTTATCCTTTGGGAGTAGACCTTCCGGGTGAGAAGCGTGGGATGAT
CCCGAATGCTGCTTATTATGATAAGAACTATCGGGGATCATGGAACGGACTGACGATTATTTCTATTTCCATCGGCCAGG
GGGAAGTGACGGCTACTCCGTTGCAGATTGCTAACCTTGGGGCTACTATTGCTAACCGGGGCTATTATATTACTCCGCAT
GTAGTGAAAGAAGTGGAAGACGAGCCGTTGGATACCCTGTATACGACCAAACGTTATACTAAAGTAAGCCGTGAACATTA
TCAGACGGTAGTCGAAGGTATGCGTTCGGCAGTATTGGGAGGAACTTGCCGTAATGCGAATATTCCGGGAATTGAAGTGT
GTGGCAAGACAGGTACGGCGCAAAACCGTGGAAAGGACCATTCTGCTTTTATGGGGTTTGCACCAATGAATGATCCTAAG
ATTGCCGTGGTAGTCTATGTAGAGAATGGAGGTTGGGGAGCAACCTATGGTGTACCTATCGGTGCATTGATAATGGAGAA
ATATTTAAAAGGCGAACTTTCACCGGAAAGTGAGGCGAAAGCGGCGGAAATACAAAACAGACGAATAGATTATGGCATAC
ACGAACGATAG

Upstream 100 bases:

>100_bases
CATTCTTCAACCTGCCTGTCTTATTGTTGAAAATATTGACAGATGCCTCAATAACCATCATTTGTATATTGTGTGCTGAG
GCAATCAGGAGGAAAAAATA

Downstream 100 bases:

>100_bases
TATATGGAAATCAGTGGACTGGATGACTATCTGCATTTATCTGATGCTGGTTATCTTTGGATGGTTCAGCGTATGTGGAG
CCAGCTATGATTATGGTGAG

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MERDYNLEKRKYVIGASVIVIVLIYLIRLFTLQIMSEDYKKNADSNAFLNKTQYPSRGVMYDRNGNLLVYNQPAYDVTMV
MKEVHNLDTLDLCKTLNITPDYFKKRIREIKDRRSNPGYSPYTHQVFMTQLSAEECGVFQEKLFKFPGFYIQRRTIRQYN
YNAAAHVLGDIAEVSKGDIEADDYYVRGDFIGKQGVERSYEKQLRGEKGVEILLRDARGRIQGRYMDGKYDKTPVPGKNL
KLGIDIELQMLGERLLEGKIGSIVAIEPSTGEILCMVSAPTFDPRLMVGRQRGKNHLELARDSWKPLLNRSIMGQFPPGS
TFKTTQALTFLQEGIITPQTAYSCYHGFVYAGLRVGCHSHGSPLPLVPAIATSCNGYFCWGLFHMIGAKKKYGSVQTAMN
TWRDYMVSMGFGYPLGVDLPGEKRGMIPNAAYYDKNYRGSWNGLTIISISIGQGEVTATPLQIANLGATIANRGYYITPH
VVKEVEDEPLDTLYTTKRYTKVSREHYQTVVEGMRSAVLGGTCRNANIPGIEVCGKTGTAQNRGKDHSAFMGFAPMNDPK
IAVVVYVENGGWGATYGVPIGALIMEKYLKGELSPESEAKAAEIQNRRIDYGIHER

Sequences:

>Translated_616_residues
MERDYNLEKRKYVIGASVIVIVLIYLIRLFTLQIMSEDYKKNADSNAFLNKTQYPSRGVMYDRNGNLLVYNQPAYDVTMV
MKEVHNLDTLDLCKTLNITPDYFKKRIREIKDRRSNPGYSPYTHQVFMTQLSAEECGVFQEKLFKFPGFYIQRRTIRQYN
YNAAAHVLGDIAEVSKGDIEADDYYVRGDFIGKQGVERSYEKQLRGEKGVEILLRDARGRIQGRYMDGKYDKTPVPGKNL
KLGIDIELQMLGERLLEGKIGSIVAIEPSTGEILCMVSAPTFDPRLMVGRQRGKNHLELARDSWKPLLNRSIMGQFPPGS
TFKTTQALTFLQEGIITPQTAYSCYHGFVYAGLRVGCHSHGSPLPLVPAIATSCNGYFCWGLFHMIGAKKKYGSVQTAMN
TWRDYMVSMGFGYPLGVDLPGEKRGMIPNAAYYDKNYRGSWNGLTIISISIGQGEVTATPLQIANLGATIANRGYYITPH
VVKEVEDEPLDTLYTTKRYTKVSREHYQTVVEGMRSAVLGGTCRNANIPGIEVCGKTGTAQNRGKDHSAFMGFAPMNDPK
IAVVVYVENGGWGATYGVPIGALIMEKYLKGELSPESEAKAAEIQNRRIDYGIHER
>Mature_616_residues
MERDYNLEKRKYVIGASVIVIVLIYLIRLFTLQIMSEDYKKNADSNAFLNKTQYPSRGVMYDRNGNLLVYNQPAYDVTMV
MKEVHNLDTLDLCKTLNITPDYFKKRIREIKDRRSNPGYSPYTHQVFMTQLSAEECGVFQEKLFKFPGFYIQRRTIRQYN
YNAAAHVLGDIAEVSKGDIEADDYYVRGDFIGKQGVERSYEKQLRGEKGVEILLRDARGRIQGRYMDGKYDKTPVPGKNL
KLGIDIELQMLGERLLEGKIGSIVAIEPSTGEILCMVSAPTFDPRLMVGRQRGKNHLELARDSWKPLLNRSIMGQFPPGS
TFKTTQALTFLQEGIITPQTAYSCYHGFVYAGLRVGCHSHGSPLPLVPAIATSCNGYFCWGLFHMIGAKKKYGSVQTAMN
TWRDYMVSMGFGYPLGVDLPGEKRGMIPNAAYYDKNYRGSWNGLTIISISIGQGEVTATPLQIANLGATIANRGYYITPH
VVKEVEDEPLDTLYTTKRYTKVSREHYQTVVEGMRSAVLGGTCRNANIPGIEVCGKTGTAQNRGKDHSAFMGFAPMNDPK
IAVVVYVENGGWGATYGVPIGALIMEKYLKGELSPESEAKAAEIQNRRIDYGIHER

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=622, Percent_Identity=27.3311897106109, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786272, Length=617, Percent_Identity=24.9594813614263, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69174; Mature: 69174

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDYNLEKRKYVIGASVIVIVLIYLIRLFTLQIMSEDYKKNADSNAFLNKTQYPSRGVM
CCCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCEE
YDRNGNLLVYNQPAYDVTMVMKEVHNLDTLDLCKTLNITPDYFKKRIREIKDRRSNPGYS
EECCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
PYTHQVFMTQLSAEECGVFQEKLFKFPGFYIQRRTIRQYNYNAAAHVLGDIAEVSKGDIE
CCHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCC
ADDYYVRGDFIGKQGVERSYEKQLRGEKGVEILLRDARGRIQGRYMDGKYDKTPVPGKNL
CCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCE
KLGIDIELQMLGERLLEGKIGSIVAIEPSTGEILCMVSAPTFDPRLMVGRQRGKNHLELA
EEEEEEHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCHHHHH
RDSWKPLLNRSIMGQFPPGSTFKTTQALTFLQEGIITPQTAYSCYHGFVYAGLRVGCHSH
HHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
GSPLPLVPAIATSCNGYFCWGLFHMIGAKKKYGSVQTAMNTWRDYMVSMGFGYPLGVDLP
CCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
GEKRGMIPNAAYYDKNYRGSWNGLTIISISIGQGEVTATPLQIANLGATIANRGYYITPH
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEECCEEHHHCCHHHHCCCEEECHH
VVKEVEDEPLDTLYTTKRYTKVSREHYQTVVEGMRSAVLGGTCRNANIPGIEVCGKTGTA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
QNRGKDHSAFMGFAPMNDPKIAVVVYVENGGWGATYGVPIGALIMEKYLKGELSPESEAK
CCCCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHH
AAEIQNRRIDYGIHER
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MERDYNLEKRKYVIGASVIVIVLIYLIRLFTLQIMSEDYKKNADSNAFLNKTQYPSRGVM
CCCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCEE
YDRNGNLLVYNQPAYDVTMVMKEVHNLDTLDLCKTLNITPDYFKKRIREIKDRRSNPGYS
EECCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
PYTHQVFMTQLSAEECGVFQEKLFKFPGFYIQRRTIRQYNYNAAAHVLGDIAEVSKGDIE
CCHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCC
ADDYYVRGDFIGKQGVERSYEKQLRGEKGVEILLRDARGRIQGRYMDGKYDKTPVPGKNL
CCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCE
KLGIDIELQMLGERLLEGKIGSIVAIEPSTGEILCMVSAPTFDPRLMVGRQRGKNHLELA
EEEEEEHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCHHHHH
RDSWKPLLNRSIMGQFPPGSTFKTTQALTFLQEGIITPQTAYSCYHGFVYAGLRVGCHSH
HHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
GSPLPLVPAIATSCNGYFCWGLFHMIGAKKKYGSVQTAMNTWRDYMVSMGFGYPLGVDLP
CCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
GEKRGMIPNAAYYDKNYRGSWNGLTIISISIGQGEVTATPLQIANLGATIANRGYYITPH
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEECCEEHHHCCHHHHCCCEEECHH
VVKEVEDEPLDTLYTTKRYTKVSREHYQTVVEGMRSAVLGGTCRNANIPGIEVCGKTGTA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
QNRGKDHSAFMGFAPMNDPKIAVVVYVENGGWGATYGVPIGALIMEKYLKGELSPESEAK
CCCCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHH
AAEIQNRRIDYGIHER
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920 [H]