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The map label for this gene is rhgT [H]

Identifier: 150004369

GI number: 150004369

Start: 2352154

End: 2353878

Strand: Direct

Name: rhgT [H]

Synonym: BVU_1815

Alternate gene names: 150004369

Gene position: 2352154-2353878 (Clockwise)

Preceding gene: 150004368

Following gene: 150004370

Centisome position: 45.56

GC content: 42.55

Gene sequence:

>1725_bases
ATGAAAATTAAGATGTTGATGGCATTTGTTGCCTTGTTGTTTTCTGCTTTTACGGCAGACCGCACAATTACTATCTTTAT
GATAGGTGACTCGACTATGGCTAACAAACCCTTAGAGGGAGGTAATCAGGAACGTGGATGGGGCCATGTGCTGGGAGGAT
ATTTCAGTGAGAATATTCGTGTGGAAAACCATGCTAGAAATGGTCGGAGTTCTAAAAGTTTTATTGATGAAGGATTGTGG
GAGGTAGTAATTAATAAAGTAAAGCCGGGTGATTATGTTTTTATTCAGTTTGGGCATAATGATGAGAAGGCCGATGAGAA
ACGTCATACTGATCCCGGATCTACTTTTGATGCCAATCTACGCCGTTTTGTAAAAGAAACACGTGCTAAAGGGGGAATTC
CCGTGTTGTTTAATGCCATTGTCCGTCGTAATTTCCGGAATAATAAAAATGCGGTAGCCGAAGATGATGTGCGCAAGGAT
TTGTCAAAGGGGAGTGTTTCTCAAGATGGAGAAGTGTTGATTGATACTCATGGAAAATATTTGGAGTCTCCGCGTAACGT
GGCAAAAGAGTTGGATGTGCCTTTTGTGGATATGAACAAGATAACGCATGATTTGGTGCAGGAGATGGGACCGGAGGCTT
CAAAAAAATTGTTCATGTGGATTCCTGAAGGTGTGTGTGCAGCTTGTCCGAAAGGGCGTGAGGATAATACGCACCTGAAT
GTATATGGGGCGCGTACTATTGCTGGTTTGACGGTGGATGCTATAGCTAAAGAGGTTCCTGCATTGGCGCCTTTTGTACG
TCATTATGATTTTGTAGTGGCAAAAGATGGCAGTGGTGATTTCTTTACCATTCAGGAAGCGATTCATGCTGTCCCCGATT
TTCGTAAAGCAGGACGCACTACTATTCTAGTCCGTAAAGGGGTATATAAAGAAAAAGTGGTTATTCCTGAAAGCAAGATT
AGTGTTTCTTTGATAGGTGAGGACGGAGCAATTCTGACAAATGATGATTTTGCTGCTAAAAAGAACTATTTCGGAGAAGA
AATGTCTACCTCCGGTTCATCAACCTGTTATATCTATGCTCCCGATTTCTATGCTGAGAATATCACTTTCGAGAATAGTG
CCGGCAGAGTGGGGCAGGCAGTGGCCTGTTTTGTTAGTGGAGATCGCGCTTATTTCAAAAACTGTCGTTTTTTGGGAAAT
CAGGATACTTTGTACACTTATGGCAAAGACAGTCGTCAGTTTTATGACCATTGTTATATAGAGGGTACAGTGGATTTTAT
TTTTGGCTGGTCTACGGCATTGTTTAAGGATTGCACCATCCATAGTTTGGGTGATGGGTATGTTACCGCTCCTTCTACAG
ATCAAGGCAAAAAATATGGCTATGTTTTTATAGGCTGTAAATTGACGGGTGTGGCTGAGGCTCAGAAGGTGTATTTGTCC
CGTCCTTGGCGTCCTTATGCACAGGCTGTTTATATTCATTGTGATTTGGGAAAGCATATTCTTCCGGTGGGTTGGAACAA
CTGGGGCAAAAAAGAAAATGAGGAAACCGTATTCTATGCTGAATACCGGAATACGGGAGAAGGGGCGGCTACCGCTTCGC
GTGCTTCGTTTGGAAAGCAGCTGAACGATATCAGTAATTATAATGAAGCACAGATTTTGGCAGGTGATGACGGATGGAAT
CCGGTGGAAAATGGAAATGTGTTGTTGCAGAATTTAAAGAGATAA

Upstream 100 bases:

>100_bases
TCTGTTGACTACCAAGTTCTATGATTTGCCCCATTTTTGCAGTAAGGAGATACAGGATGATATACTTGAGTTCTTTAATC
AAAATTTAAAATAGAAAGCG

Downstream 100 bases:

>100_bases
GCTGTTCTTTCTACCAGTAATAATACTTTGTCACTGTAGGGAGGAATTCTATTTGTTCCTATGGAAAGTGTCAGGGAAGA
GTAGAATGATAAAGGGGGGA

Product: carbohydrate esterase family 8 protein

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKIKMLMAFVALLFSAFTADRTITIFMIGDSTMANKPLEGGNQERGWGHVLGGYFSENIRVENHARNGRSSKSFIDEGLW
EVVINKVKPGDYVFIQFGHNDEKADEKRHTDPGSTFDANLRRFVKETRAKGGIPVLFNAIVRRNFRNNKNAVAEDDVRKD
LSKGSVSQDGEVLIDTHGKYLESPRNVAKELDVPFVDMNKITHDLVQEMGPEASKKLFMWIPEGVCAACPKGREDNTHLN
VYGARTIAGLTVDAIAKEVPALAPFVRHYDFVVAKDGSGDFFTIQEAIHAVPDFRKAGRTTILVRKGVYKEKVVIPESKI
SVSLIGEDGAILTNDDFAAKKNYFGEEMSTSGSSTCYIYAPDFYAENITFENSAGRVGQAVACFVSGDRAYFKNCRFLGN
QDTLYTYGKDSRQFYDHCYIEGTVDFIFGWSTALFKDCTIHSLGDGYVTAPSTDQGKKYGYVFIGCKLTGVAEAQKVYLS
RPWRPYAQAVYIHCDLGKHILPVGWNNWGKKENEETVFYAEYRNTGEGAATASRASFGKQLNDISNYNEAQILAGDDGWN
PVENGNVLLQNLKR

Sequences:

>Translated_574_residues
MKIKMLMAFVALLFSAFTADRTITIFMIGDSTMANKPLEGGNQERGWGHVLGGYFSENIRVENHARNGRSSKSFIDEGLW
EVVINKVKPGDYVFIQFGHNDEKADEKRHTDPGSTFDANLRRFVKETRAKGGIPVLFNAIVRRNFRNNKNAVAEDDVRKD
LSKGSVSQDGEVLIDTHGKYLESPRNVAKELDVPFVDMNKITHDLVQEMGPEASKKLFMWIPEGVCAACPKGREDNTHLN
VYGARTIAGLTVDAIAKEVPALAPFVRHYDFVVAKDGSGDFFTIQEAIHAVPDFRKAGRTTILVRKGVYKEKVVIPESKI
SVSLIGEDGAILTNDDFAAKKNYFGEEMSTSGSSTCYIYAPDFYAENITFENSAGRVGQAVACFVSGDRAYFKNCRFLGN
QDTLYTYGKDSRQFYDHCYIEGTVDFIFGWSTALFKDCTIHSLGDGYVTAPSTDQGKKYGYVFIGCKLTGVAEAQKVYLS
RPWRPYAQAVYIHCDLGKHILPVGWNNWGKKENEETVFYAEYRNTGEGAATASRASFGKQLNDISNYNEAQILAGDDGWN
PVENGNVLLQNLKR
>Mature_574_residues
MKIKMLMAFVALLFSAFTADRTITIFMIGDSTMANKPLEGGNQERGWGHVLGGYFSENIRVENHARNGRSSKSFIDEGLW
EVVINKVKPGDYVFIQFGHNDEKADEKRHTDPGSTFDANLRRFVKETRAKGGIPVLFNAIVRRNFRNNKNAVAEDDVRKD
LSKGSVSQDGEVLIDTHGKYLESPRNVAKELDVPFVDMNKITHDLVQEMGPEASKKLFMWIPEGVCAACPKGREDNTHLN
VYGARTIAGLTVDAIAKEVPALAPFVRHYDFVVAKDGSGDFFTIQEAIHAVPDFRKAGRTTILVRKGVYKEKVVIPESKI
SVSLIGEDGAILTNDDFAAKKNYFGEEMSTSGSSTCYIYAPDFYAENITFENSAGRVGQAVACFVSGDRAYFKNCRFLGN
QDTLYTYGKDSRQFYDHCYIEGTVDFIFGWSTALFKDCTIHSLGDGYVTAPSTDQGKKYGYVFIGCKLTGVAEAQKVYLS
RPWRPYAQAVYIHCDLGKHILPVGWNNWGKKENEETVFYAEYRNTGEGAATASRASFGKQLNDISNYNEAQILAGDDGWN
PVENGNVLLQNLKR

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 63982; Mature: 63982

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00503 PECTINESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKMLMAFVALLFSAFTADRTITIFMIGDSTMANKPLEGGNQERGWGHVLGGYFSENIR
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHCCCCCCCCCCCCCCHHHCCCHHCCCCE
VENHARNGRSSKSFIDEGLWEVVINKVKPGDYVFIQFGHNDEKADEKRHTDPGSTFDANL
EECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCHHHHH
RRFVKETRAKGGIPVLFNAIVRRNFRNNKNAVAEDDVRKDLSKGSVSQDGEVLIDTHGKY
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHH
LESPRNVAKELDVPFVDMNKITHDLVQEMGPEASKKLFMWIPEGVCAACPKGREDNTHLN
HCCHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCCCCCCCEEE
VYGARTIAGLTVDAIAKEVPALAPFVRHYDFVVAKDGSGDFFTIQEAIHAVPDFRKAGRT
EEECCEECCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEHHHHHHHCCCHHHCCCE
TILVRKGVYKEKVVIPESKISVSLIGEDGAILTNDDFAAKKNYFGEEMSTSGSSTCYIYA
EEEEECCCCCCEEECCCCCEEEEEECCCCCEEECCCCHHHHHCCCCHHCCCCCCEEEEEC
PDFYAENITFENSAGRVGQAVACFVSGDRAYFKNCRFLGNQDTLYTYGKDSRQFYDHCYI
CCCEECCEEEECCCCCCCCEEEEEEECCHHHHHCCEEECCCCEEEEECCCCHHHHHEEEE
EGTVDFIFGWSTALFKDCTIHSLGDGYVTAPSTDQGKKYGYVFIGCKLTGVAEAQKVYLS
EEEEEEEEECHHHHHHCCEEEECCCCEEECCCCCCCCEEEEEEEEEEEECCHHHHHHHHC
RPWRPYAQAVYIHCDLGKHILPVGWNNWGKKENEETVFYAEYRNTGEGAATASRASFGKQ
CCCCCCCEEEEEEEECCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
LNDISNYNEAQILAGDDGWNPVENGNVLLQNLKR
HHHHCCCCCEEEEECCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MKIKMLMAFVALLFSAFTADRTITIFMIGDSTMANKPLEGGNQERGWGHVLGGYFSENIR
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHCCCCCCCCCCCCCCHHHCCCHHCCCCE
VENHARNGRSSKSFIDEGLWEVVINKVKPGDYVFIQFGHNDEKADEKRHTDPGSTFDANL
EECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCHHHHH
RRFVKETRAKGGIPVLFNAIVRRNFRNNKNAVAEDDVRKDLSKGSVSQDGEVLIDTHGKY
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHH
LESPRNVAKELDVPFVDMNKITHDLVQEMGPEASKKLFMWIPEGVCAACPKGREDNTHLN
HCCHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCCCCCCCEEE
VYGARTIAGLTVDAIAKEVPALAPFVRHYDFVVAKDGSGDFFTIQEAIHAVPDFRKAGRT
EEECCEECCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEHHHHHHHCCCHHHCCCE
TILVRKGVYKEKVVIPESKISVSLIGEDGAILTNDDFAAKKNYFGEEMSTSGSSTCYIYA
EEEEECCCCCCEEECCCCCEEEEEECCCCCEEECCCCHHHHHCCCCHHCCCCCCEEEEEC
PDFYAENITFENSAGRVGQAVACFVSGDRAYFKNCRFLGNQDTLYTYGKDSRQFYDHCYI
CCCEECCEEEECCCCCCCCEEEEEEECCHHHHHCCEEECCCCEEEEECCCCHHHHHEEEE
EGTVDFIFGWSTALFKDCTIHSLGDGYVTAPSTDQGKKYGYVFIGCKLTGVAEAQKVYLS
EEEEEEEEECHHHHHHCCEEEECCCCEEECCCCCCCCEEEEEEEEEEEECCHHHHHHHHC
RPWRPYAQAVYIHCDLGKHILPVGWNNWGKKENEETVFYAEYRNTGEGAATASRASFGKQ
CCCCCCCEEEEEEEECCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
LNDISNYNEAQILAGDDGWNPVENGNVLLQNLKR
HHHHCCCCCEEEEECCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]