Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 150004215

GI number: 150004215

Start: 2139648

End: 2141066

Strand: Reverse

Name: cca [H]

Synonym: BVU_1656

Alternate gene names: 150004215

Gene position: 2141066-2139648 (Counterclockwise)

Preceding gene: 150004217

Following gene: 150004214

Centisome position: 41.47

GC content: 47.15

Gene sequence:

>1419_bases
ATGGAACTAAAAGAACACTTTAATAATAAGATATTCAAGTTAATCTCCGAAACGGCAGACGAACTGGGATTGGAATGTTA
CGTTGTAGGAGGTTATGTACGAGATATATTCCTGAAGCGTCCCTCGAAGGATATAGACGTTGTAGTAGTAGGGAGCGGAA
TTGAAATGGCACAGGCCTTCGGCAGGAAGCTGGGACGTGGGGCACGCGTTTCTGTTTTTAAAAACTTCGGTACGGCACAA
ATCAAGTACCATGATACGGAAGTGGAGTTTGTGGGGGCCCGCAAGGAATCATACAGCCATGACAGCCGCAAGCCTGTAGT
AGAGAACGGCACCTTGGAGGATGACCAAAACCGGCGCGATTTCACGATCAATGCCATGGCAGTGTGCCTGAACAAAGCAC
GTTTCGGTGAACTGGTAGATCCTTTTAACGGGCTGGACGATTTGAAAGAGAGAACTATCCGTACACCGCTGGACCCGGAC
ATCACTTTCAGTGACGATCCGCTCCGCATGATGCGTTGTGTCCGGTTTGCCACACAACTGAACTTCTATATTGACGACAC
CACTTTCGAAGCACTGTCACGCAACAAGGAACGCATTCATATTATCTCCAAAGAGCGCATCGCCGACGAGTTGAACAAGA
TCCTGCTCGCCCCCATACCGTCCAAAGGCTTTATAGAACTGGACCGCTGCGGACTGCTGCCTTTGATTTTTCCCGAACTG
GTGGCGCTGCAAGGAGTGGAAACCCGAAACGGGCGTGCCCATAAAGACAACTTCTATCATACCCTGGAAGTTGTGGACAA
TATTTCCAAACATACCGACAACCTATGGCTGCGTTGGGCTACGCTGTTGCATGATATAGGCAAGCCACGTACCAAACGCT
GGGAGCCACGTATAGGATGGACTTTCCATAATCATAACTTTATCGGTGAAAAAATGGTTCCGGATCTCTTCCGCAAGATG
AAACTGCCCATGAATGAGAAGATGAAATATGTCCAAAAGTTGGTCAGCCTGCACATGCGCCCCATTGTTATCGCAGATGA
TGTAGTAACCGATTCCGCCGTGCGACGTCTGCTTTTTGAAGCCGGAGATGATATTGACGACTTGATGATGCTGTGCGAAG
CGGACATCACTTCAAAAAATGAAGCACGAAAACAAAAATTCCTGGATAACTTCAAACTGGTACGCCAGAAGCTGAAAGAC
CTGGAAGAAAAAGACCGGATACGCAATTTCCAACCTCCGGTAGACGGAGAAGAAATTATGCGGATATTCGACCTGCAGCC
CTGCCGTGAAATAGGCAGTCTGAAAAGTTCTATCAAGGATGCCATACTGGATGGAATCATCCCTAATGAACATGATGCTG
CTTTAGAACACATGTTGAAGAAAGCAAAAAGCATGGGATTAACTCCAAAAAACTTATAA

Upstream 100 bases:

>100_bases
TACTTGATCGTTTTATATGCAGGCAAAAATACAATTTAATTGCGTGATTTCTCCTTATCCTGCGCAAAAAATCGTAAATT
TGCCCCTCTTTAAGATGAAT

Downstream 100 bases:

>100_bases
GAAATTGACCATACACTATACCAAAGGAGAAGAGTGGGCCAATACCCTGTCACATGGCGTTGGTATCCTGATAGGTATCG
CCGGCGGCGGTTATCTGTTG

Product: putative polyA polymerase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MELKEHFNNKIFKLISETADELGLECYVVGGYVRDIFLKRPSKDIDVVVVGSGIEMAQAFGRKLGRGARVSVFKNFGTAQ
IKYHDTEVEFVGARKESYSHDSRKPVVENGTLEDDQNRRDFTINAMAVCLNKARFGELVDPFNGLDDLKERTIRTPLDPD
ITFSDDPLRMMRCVRFATQLNFYIDDTTFEALSRNKERIHIISKERIADELNKILLAPIPSKGFIELDRCGLLPLIFPEL
VALQGVETRNGRAHKDNFYHTLEVVDNISKHTDNLWLRWATLLHDIGKPRTKRWEPRIGWTFHNHNFIGEKMVPDLFRKM
KLPMNEKMKYVQKLVSLHMRPIVIADDVVTDSAVRRLLFEAGDDIDDLMMLCEADITSKNEARKQKFLDNFKLVRQKLKD
LEEKDRIRNFQPPVDGEEIMRIFDLQPCREIGSLKSSIKDAILDGIIPNEHDAALEHMLKKAKSMGLTPKNL

Sequences:

>Translated_472_residues
MELKEHFNNKIFKLISETADELGLECYVVGGYVRDIFLKRPSKDIDVVVVGSGIEMAQAFGRKLGRGARVSVFKNFGTAQ
IKYHDTEVEFVGARKESYSHDSRKPVVENGTLEDDQNRRDFTINAMAVCLNKARFGELVDPFNGLDDLKERTIRTPLDPD
ITFSDDPLRMMRCVRFATQLNFYIDDTTFEALSRNKERIHIISKERIADELNKILLAPIPSKGFIELDRCGLLPLIFPEL
VALQGVETRNGRAHKDNFYHTLEVVDNISKHTDNLWLRWATLLHDIGKPRTKRWEPRIGWTFHNHNFIGEKMVPDLFRKM
KLPMNEKMKYVQKLVSLHMRPIVIADDVVTDSAVRRLLFEAGDDIDDLMMLCEADITSKNEARKQKFLDNFKLVRQKLKD
LEEKDRIRNFQPPVDGEEIMRIFDLQPCREIGSLKSSIKDAILDGIIPNEHDAALEHMLKKAKSMGLTPKNL
>Mature_472_residues
MELKEHFNNKIFKLISETADELGLECYVVGGYVRDIFLKRPSKDIDVVVVGSGIEMAQAFGRKLGRGARVSVFKNFGTAQ
IKYHDTEVEFVGARKESYSHDSRKPVVENGTLEDDQNRRDFTINAMAVCLNKARFGELVDPFNGLDDLKERTIRTPLDPD
ITFSDDPLRMMRCVRFATQLNFYIDDTTFEALSRNKERIHIISKERIADELNKILLAPIPSKGFIELDRCGLLPLIFPEL
VALQGVETRNGRAHKDNFYHTLEVVDNISKHTDNLWLRWATLLHDIGKPRTKRWEPRIGWTFHNHNFIGEKMVPDLFRKM
KLPMNEKMKYVQKLVSLHMRPIVIADDVVTDSAVRRLLFEAGDDIDDLMMLCEADITSKNEARKQKFLDNFKLVRQKLKD
LEEKDRIRNFQPPVDGEEIMRIFDLQPCREIGSLKSSIKDAILDGIIPNEHDAALEHMLKKAKSMGLTPKNL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=201, Percent_Identity=30.8457711442786, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789436, Length=383, Percent_Identity=33.1592689295039, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87081691, Length=234, Percent_Identity=30.3418803418803, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71995920, Length=200, Percent_Identity=29, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321016, Length=233, Percent_Identity=34.7639484978541, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI281360112, Length=217, Percent_Identity=32.7188940092166, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357337, Length=217, Percent_Identity=32.7188940092166, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54411; Mature: 54411

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKEHFNNKIFKLISETADELGLECYVVGGYVRDIFLKRPSKDIDVVVVGSGIEMAQAF
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCCEEEEECCCHHHHHHH
GRKLGRGARVSVFKNFGTAQIKYHDTEVEFVGARKESYSHDSRKPVVENGTLEDDQNRRD
HHHHCCCCEEEEECCCCCEEEEEECCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCC
FTINAMAVCLNKARFGELVDPFNGLDDLKERTIRTPLDPDITFSDDPLRMMRCVRFATQL
EEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
NFYIDDTTFEALSRNKERIHIISKERIADELNKILLAPIPSKGFIELDRCGLLPLIFPEL
HEEECCHHHHHHHCCCCEEEEHHHHHHHHHHHHEEEECCCCCCCEEECCCCCHHHHHHHH
VALQGVETRNGRAHKDNFYHTLEVVDNISKHTDNLWLRWATLLHDIGKPRTKRWEPRIGW
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
TFHNHNFIGEKMVPDLFRKMKLPMNEKMKYVQKLVSLHMRPIVIADDVVTDSAVRRLLFE
EECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHH
AGDDIDDLMMLCEADITSKNEARKQKFLDNFKLVRQKLKDLEEKDRIRNFQPPVDGEEIM
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
RIFDLQPCREIGSLKSSIKDAILDGIIPNEHDAALEHMLKKAKSMGLTPKNL
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MELKEHFNNKIFKLISETADELGLECYVVGGYVRDIFLKRPSKDIDVVVVGSGIEMAQAF
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCCEEEEECCCHHHHHHH
GRKLGRGARVSVFKNFGTAQIKYHDTEVEFVGARKESYSHDSRKPVVENGTLEDDQNRRD
HHHHCCCCEEEEECCCCCEEEEEECCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCC
FTINAMAVCLNKARFGELVDPFNGLDDLKERTIRTPLDPDITFSDDPLRMMRCVRFATQL
EEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
NFYIDDTTFEALSRNKERIHIISKERIADELNKILLAPIPSKGFIELDRCGLLPLIFPEL
HEEECCHHHHHHHCCCCEEEEHHHHHHHHHHHHEEEECCCCCCCEEECCCCCHHHHHHHH
VALQGVETRNGRAHKDNFYHTLEVVDNISKHTDNLWLRWATLLHDIGKPRTKRWEPRIGW
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
TFHNHNFIGEKMVPDLFRKMKLPMNEKMKYVQKLVSLHMRPIVIADDVVTDSAVRRLLFE
EECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHH
AGDDIDDLMMLCEADITSKNEARKQKFLDNFKLVRQKLKDLEEKDRIRNFQPPVDGEEIM
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
RIFDLQPCREIGSLKSSIKDAILDGIIPNEHDAALEHMLKKAKSMGLTPKNL
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA