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The map label for this gene is waaA [H]

Identifier: 150004041

GI number: 150004041

Start: 1956109

End: 1957329

Strand: Direct

Name: waaA [H]

Synonym: BVU_1476

Alternate gene names: 150004041

Gene position: 1956109-1957329 (Clockwise)

Preceding gene: 150004040

Following gene: 150004042

Centisome position: 37.89

GC content: 44.23

Gene sequence:

>1221_bases
ATGATTTATAACCTAGTCATCTATATTTATCTATTCGGGGTGAAGGTTGCCGGCCTGTTCAGTGCCAAGCCCGCCAAAAT
GGTGAAAGGACACCGGGAAGTATTTGATATCTTGCGTAACAAGATAGACAAAAATGCCCGTTATATTTGGTTTCATGCAG
CTTCATTGGGGGAGTTCGAACAGGGACGCCCTTTGATAGAACGTATCCGTAAGGAGCATCCCGAATATAAAATATTGCAG
ACTTTCTTTTCTCCCTCAGGGTATGAAGTGAGAAAGAACTACCAGGGGGCAGACCTTGTCTGCTATCTGCCTTTCGACAC
TCCGCGTAATGTCCGTCGGTTTGTAGAATTGGCTAATCCCTGTATGGTATTTTTTATCAAGTACGAATTTTGGCAGAATT
ACTTGAATGAGTTGCATAGGAGAGGTATTCCCACCTATAGCGTATCTTCCATTTTCCGTCCCAATCAGATTTTTTTCCGT
TGGTATGGGAAGAGATATAGTGAGGTGCTGAGGACTTTTGCTCATTTGTTTGTACAGAACGAAGTTTCCAAAGAATTGCT
GGCGACAATTGGCGTGACCGATGTGACAGTTGTGGGCGATACTCGTTTTGACCGCGTGCTGGATATCTGCCATCAAGCCA
AGCAGCTTCCGCTGGTGGAGAAGTTCAAAGGTGATTCGTTGACTTTTGTTGCAGGCAGCTCATGGGGACCGGATGAGGAT
ATTTTTATCAAGTATTTCAATGAGCATCCTGAGATGAAGCTGGTTATTGCTCCTCATGTGGTGAGCGACAGTCATTTGAA
GGAGATTTTAGATAAGGTGAAACGTCCGTGCATCCGCTATACCGAGGCTACGGAAGAAAATGTGACACAGGCCGATTGTT
TGATTATCGACTGTTACGGACTGCTGTCCTCCATTTACCGATATGGTGAGATTTCGTATATTGGGGGTGGCTTTGGAGTT
GGAATTCATAATGTGCTGGAAGCGGCTGTATATGGTATTCCTGTGATATTCGGACCTAACAACAAGAAATTCCGCGAGGC
GCAACATTTGTTGGAGCAGAAAGGCGGTTTTGAAGTAACCGGTTATGATGATTTTAAACGGTTGATGGACAAATTCCTGT
CGGATGAGGCTTATTTGCAACAAGCAGGGAAAGCTGCCGGAAATTATGTGAACCATAATGCCGGAGCATTAGAAAAGATA
ATGAAAGATATCACTCTCTGA

Upstream 100 bases:

>100_bases
AGGTCCGCATATGTTTGATATTTCAGCCGTTTTAGGGAAAGAAGAAACATTGCGACGCATTCGTCGGGCTGTGGAAGTGC
TCAAGTAATAGCCGGTAGTT

Downstream 100 bases:

>100_bases
ATGTTTCAAGGTGATTTGAGTATATGCAGGTTATAGAGAAGATTTGGCTCTATAACCTGTTTTTTTCCTTCAAATCCTTC
ATTTCAAGGTAATTTATTGT

Product: glycosyl transferase family protein

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MIYNLVIYIYLFGVKVAGLFSAKPAKMVKGHREVFDILRNKIDKNARYIWFHAASLGEFEQGRPLIERIRKEHPEYKILQ
TFFSPSGYEVRKNYQGADLVCYLPFDTPRNVRRFVELANPCMVFFIKYEFWQNYLNELHRRGIPTYSVSSIFRPNQIFFR
WYGKRYSEVLRTFAHLFVQNEVSKELLATIGVTDVTVVGDTRFDRVLDICHQAKQLPLVEKFKGDSLTFVAGSSWGPDED
IFIKYFNEHPEMKLVIAPHVVSDSHLKEILDKVKRPCIRYTEATEENVTQADCLIIDCYGLLSSIYRYGEISYIGGGFGV
GIHNVLEAAVYGIPVIFGPNNKKFREAQHLLEQKGGFEVTGYDDFKRLMDKFLSDEAYLQQAGKAAGNYVNHNAGALEKI
MKDITL

Sequences:

>Translated_406_residues
MIYNLVIYIYLFGVKVAGLFSAKPAKMVKGHREVFDILRNKIDKNARYIWFHAASLGEFEQGRPLIERIRKEHPEYKILQ
TFFSPSGYEVRKNYQGADLVCYLPFDTPRNVRRFVELANPCMVFFIKYEFWQNYLNELHRRGIPTYSVSSIFRPNQIFFR
WYGKRYSEVLRTFAHLFVQNEVSKELLATIGVTDVTVVGDTRFDRVLDICHQAKQLPLVEKFKGDSLTFVAGSSWGPDED
IFIKYFNEHPEMKLVIAPHVVSDSHLKEILDKVKRPCIRYTEATEENVTQADCLIIDCYGLLSSIYRYGEISYIGGGFGV
GIHNVLEAAVYGIPVIFGPNNKKFREAQHLLEQKGGFEVTGYDDFKRLMDKFLSDEAYLQQAGKAAGNYVNHNAGALEKI
MKDITL
>Mature_406_residues
MIYNLVIYIYLFGVKVAGLFSAKPAKMVKGHREVFDILRNKIDKNARYIWFHAASLGEFEQGRPLIERIRKEHPEYKILQ
TFFSPSGYEVRKNYQGADLVCYLPFDTPRNVRRFVELANPCMVFFIKYEFWQNYLNELHRRGIPTYSVSSIFRPNQIFFR
WYGKRYSEVLRTFAHLFVQNEVSKELLATIGVTDVTVVGDTRFDRVLDICHQAKQLPLVEKFKGDSLTFVAGSSWGPDED
IFIKYFNEHPEMKLVIAPHVVSDSHLKEILDKVKRPCIRYTEATEENVTQADCLIIDCYGLLSSIYRYGEISYIGGGFGV
GIHNVLEAAVYGIPVIFGPNNKKFREAQHLLEQKGGFEVTGYDDFKRLMDKFLSDEAYLQQAGKAAGNYVNHNAGALEKI
MKDITL

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=366, Percent_Identity=25.9562841530055, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 46818; Mature: 46818

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYNLVIYIYLFGVKVAGLFSAKPAKMVKGHREVFDILRNKIDKNARYIWFHAASLGEFE
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
QGRPLIERIRKEHPEYKILQTFFSPSGYEVRKNYQGADLVCYLPFDTPRNVRRFVELANP
CCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCC
CMVFFIKYEFWQNYLNELHRRGIPTYSVSSIFRPNQIFFRWYGKRYSEVLRTFAHLFVQN
HHEEHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EVSKELLATIGVTDVTVVGDTRFDRVLDICHQAKQLPLVEKFKGDSLTFVAGSSWGPDED
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCC
IFIKYFNEHPEMKLVIAPHVVSDSHLKEILDKVKRPCIRYTEATEENVTQADCLIIDCYG
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEHHH
LLSSIYRYGEISYIGGGFGVGIHNVLEAAVYGIPVIFGPNNKKFREAQHLLEQKGGFEVT
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCEEEC
GYDDFKRLMDKFLSDEAYLQQAGKAAGNYVNHNAGALEKIMKDITL
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIYNLVIYIYLFGVKVAGLFSAKPAKMVKGHREVFDILRNKIDKNARYIWFHAASLGEFE
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
QGRPLIERIRKEHPEYKILQTFFSPSGYEVRKNYQGADLVCYLPFDTPRNVRRFVELANP
CCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCC
CMVFFIKYEFWQNYLNELHRRGIPTYSVSSIFRPNQIFFRWYGKRYSEVLRTFAHLFVQN
HHEEHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EVSKELLATIGVTDVTVVGDTRFDRVLDICHQAKQLPLVEKFKGDSLTFVAGSSWGPDED
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCC
IFIKYFNEHPEMKLVIAPHVVSDSHLKEILDKVKRPCIRYTEATEENVTQADCLIIDCYG
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEHHH
LLSSIYRYGEISYIGGGFGVGIHNVLEAAVYGIPVIFGPNNKKFREAQHLLEQKGGFEVT
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCEEEC
GYDDFKRLMDKFLSDEAYLQQAGKAAGNYVNHNAGALEKIMKDITL
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]