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The map label for this gene is gltX

Identifier: 150004040

GI number: 150004040

Start: 1954579

End: 1956096

Strand: Direct

Name: gltX

Synonym: BVU_1475

Alternate gene names: 150004040

Gene position: 1954579-1956096 (Clockwise)

Preceding gene: 150004038

Following gene: 150004041

Centisome position: 37.86

GC content: 46.38

Gene sequence:

>1518_bases
ATGTCAGAAAGAAAAGTAAGAGTTCGTTTTGCTCCGAGCCCGACGGGAGCGTTGCATATCGGCGGTGTGCGCACAGCCTT
GTATAACTATCTGTTTGCTCGCCAGCATGGTGGAGATTTAATTTTCCGTATAGAAGATACCGACTCAAATCGCTTTGTTC
CGGGTGCGGAAGAATATATCATTGAGTCGTTTAAGTGGTTGGGGATAAACTTCGACGAAGGAGTCAGCTTTGGTGGTAAC
TACGGCCCCTACCGTCAGTCTGAACGTCGTGATATTTATAAGAAATATGTACAGGTGTTGCTTGACAGCGGTAAGGCGTA
TATTGCTTTCGATACTCCGGCGGAGTTGGACGCCAAACGTCAGGAAATCTCTAATTTCCAGTACGATGCTTCTACCCGTA
TGTCTATGCGTAACTCTTTGACTCTGCCGAAAGAAGAAGTGGATGCACTGATTGCCGATGGGAAGCAATATGTGGTACGT
TTCAAGATTGAACCGAATGAAGATGTACACGTACATGATATTATCCGTGGCGAGGTAGTGATCAATTCTTCCATTTTGGA
TGATAAAGTTTTATATAAATCGGCGGATGAACTGCCTACTTATCATTTGGCAAACATTGTGGACGACCATTTGATGGAAG
TTTCCCATGTAATCCGTGGTGAGGAATGGTTGCCTTCCGCTCCGTTGCATGTCCTGCTGTACCGGGCTTTCGGCTGGGCG
GATACCATGCCCGAGTTCGCACATCTTCCCTTGTTGTTGAAACCGGATGGTAACGGTAAACTGAGCAAGCGTGATGGTGA
CCGTCTTGGCTTCCCTGTTTTCCCGTTGGAATGGCACGACCCCAAGACCGGTGAGGTGTCTTCCGGTTATCGTGAGTCGG
GTTACCTGCCCGAAGCTGTTATCAATTTCCTTGCCCTGTTGGGATGGAATCCGGGAAATGACCAGGAACTGATGTCCTTG
GATGAACTGGTGAAGCTTTTTGACCTGCACCGTTGCAGCAAAGCCGGGGCAAAATTCGACTTTGAAAAAGGAAAATGGTT
TAACCATGAATATATATTGAAGAAAAGCAATGAGGAAGTAGCCGGGCTTTTCATGCCGATCTTGAAAGAGCATGGCATTG
AAGCTCCTATGGATAAAGTGGTGACCGTGGTAGGCTTGATGAAAGATCGTGTAAGTTTTATCAAGGATTTGTGGGAAACT
TGCAAATTCTTCTTCGTCGCTCCTACGGAATATGATGAGAAGACCCGTAAAAAACGTTGGAAAGAGGACTCTCCCGAACG
TATGCTGGAGTTGGCGGACGTATTGGAGGCCTTGGACGACTTCTCGCTGGAGAATCAGGAGGCTGTGGTCATGAAATGGA
TAGAAGATAAAGGTTATCACTTAGGTAATATAATGAATGCTTTCCGTTTAACCTTGGTAGGCGAAGGTAAAGGTCCGCAT
ATGTTTGATATTTCAGCCGTTTTAGGGAAAGAAGAAACATTGCGACGCATTCGTCGGGCTGTGGAAGTGCTCAAGTAA

Upstream 100 bases:

>100_bases
TGGTCTTGAAACTTCTCATAATCGTATATTTTTGCGCGCAAAATACGAAAAAATCGGGAAATTTAGATTAATTTTGCGGC
GTATTTTATATAAAACAACA

Downstream 100 bases:

>100_bases
TAGCCGGTAGTTATGATTTATAACCTAGTCATCTATATTTATCTATTCGGGGTGAAGGTTGCCGGCCTGTTCAGTGCCAA
GCCCGCCAAAATGGTGAAAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYIIESFKWLGINFDEGVSFGGN
YGPYRQSERRDIYKKYVQVLLDSGKAYIAFDTPAELDAKRQEISNFQYDASTRMSMRNSLTLPKEEVDALIADGKQYVVR
FKIEPNEDVHVHDIIRGEVVINSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWA
DTMPEFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLEWHDPKTGEVSSGYRESGYLPEAVINFLALLGWNPGNDQELMSL
DELVKLFDLHRCSKAGAKFDFEKGKWFNHEYILKKSNEEVAGLFMPILKEHGIEAPMDKVVTVVGLMKDRVSFIKDLWET
CKFFFVAPTEYDEKTRKKRWKEDSPERMLELADVLEALDDFSLENQEAVVMKWIEDKGYHLGNIMNAFRLTLVGEGKGPH
MFDISAVLGKEETLRRIRRAVEVLK

Sequences:

>Translated_505_residues
MSERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYIIESFKWLGINFDEGVSFGGN
YGPYRQSERRDIYKKYVQVLLDSGKAYIAFDTPAELDAKRQEISNFQYDASTRMSMRNSLTLPKEEVDALIADGKQYVVR
FKIEPNEDVHVHDIIRGEVVINSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWA
DTMPEFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLEWHDPKTGEVSSGYRESGYLPEAVINFLALLGWNPGNDQELMSL
DELVKLFDLHRCSKAGAKFDFEKGKWFNHEYILKKSNEEVAGLFMPILKEHGIEAPMDKVVTVVGLMKDRVSFIKDLWET
CKFFFVAPTEYDEKTRKKRWKEDSPERMLELADVLEALDDFSLENQEAVVMKWIEDKGYHLGNIMNAFRLTLVGEGKGPH
MFDISAVLGKEETLRRIRRAVEVLK
>Mature_504_residues
SERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYIIESFKWLGINFDEGVSFGGNY
GPYRQSERRDIYKKYVQVLLDSGKAYIAFDTPAELDAKRQEISNFQYDASTRMSMRNSLTLPKEEVDALIADGKQYVVRF
KIEPNEDVHVHDIIRGEVVINSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWAD
TMPEFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLEWHDPKTGEVSSGYRESGYLPEAVINFLALLGWNPGNDQELMSLD
ELVKLFDLHRCSKAGAKFDFEKGKWFNHEYILKKSNEEVAGLFMPILKEHGIEAPMDKVVTVVGLMKDRVSFIKDLWETC
KFFFVAPTEYDEKTRKKRWKEDSPERMLELADVLEALDDFSLENQEAVVMKWIEDKGYHLGNIMNAFRLTLVGEGKGPHM
FDISAVLGKEETLRRIRRAVEVLK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=518, Percent_Identity=34.9420849420849, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1788743, Length=505, Percent_Identity=32.0792079207921, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI226510925, Length=265, Percent_Identity=31.6981132075472, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=516, Percent_Identity=35.4651162790698, Blast_Score=265, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324540, Length=522, Percent_Identity=36.3984674329502, Blast_Score=290, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6321192, Length=311, Percent_Identity=26.6881028938907, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24665321, Length=519, Percent_Identity=35.4527938342967, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_BACV8 (A6L0E8)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298784.1
- ProteinModelPortal:   A6L0E8
- SMR:   A6L0E8
- STRING:   A6L0E8
- GeneID:   5302441
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1475
- NMPDR:   fig|435590.6.peg.1393
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 57875; Mature: 57743

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 263-263

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYI
CCCCEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHH
IESFKWLGINFDEGVSFGGNYGPYRQSERRDIYKKYVQVLLDSGKAYIAFDTPAELDAKR
HHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
QEISNFQYDASTRMSMRNSLTLPKEEVDALIADGKQYVVRFKIEPNEDVHVHDIIRGEVV
HHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECEEE
INSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWA
EECCCCCHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
DTMPEFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLEWHDPKTGEVSSGYRESGYLPEAV
CCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
INFLALLGWNPGNDQELMSLDELVKLFDLHRCSKAGAKFDFEKGKWFNHEYILKKSNEEV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCHH
AGLFMPILKEHGIEAPMDKVVTVVGLMKDRVSFIKDLWETCKFFFVAPTEYDEKTRKKRW
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCC
KEDSPERMLELADVLEALDDFSLENQEAVVMKWIEDKGYHLGNIMNAFRLTLVGEGKGPH
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHCCCCHHHHHHHHEEEEEEECCCCCC
MFDISAVLGKEETLRRIRRAVEVLK
EEEHHHHHCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYI
CCCEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHH
IESFKWLGINFDEGVSFGGNYGPYRQSERRDIYKKYVQVLLDSGKAYIAFDTPAELDAKR
HHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
QEISNFQYDASTRMSMRNSLTLPKEEVDALIADGKQYVVRFKIEPNEDVHVHDIIRGEVV
HHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECEEE
INSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWA
EECCCCCHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
DTMPEFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLEWHDPKTGEVSSGYRESGYLPEAV
CCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
INFLALLGWNPGNDQELMSLDELVKLFDLHRCSKAGAKFDFEKGKWFNHEYILKKSNEEV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCHH
AGLFMPILKEHGIEAPMDKVVTVVGLMKDRVSFIKDLWETCKFFFVAPTEYDEKTRKKRW
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCC
KEDSPERMLELADVLEALDDFSLENQEAVVMKWIEDKGYHLGNIMNAFRLTLVGEGKGPH
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHCCCCHHHHHHHHEEEEEEECCCCCC
MFDISAVLGKEETLRRIRRAVEVLK
EEEHHHHHCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA