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The map label for this gene is priA [H]

Identifier: 150004036

GI number: 150004036

Start: 1948767

End: 1951229

Strand: Direct

Name: priA [H]

Synonym: BVU_1471

Alternate gene names: 150004036

Gene position: 1948767-1951229 (Clockwise)

Preceding gene: 150004035

Following gene: 150004037

Centisome position: 37.74

GC content: 45.88

Gene sequence:

>2463_bases
ATGAAAAAATTCGTAGATGTCATAGTTCCATTACCTATTGCCAACCAATACACTTATTCCCTTCCTCAGGAGATGGAAGA
GATGGTACAGATTGGTTGTCGGGTGGTTGTTCCGTTTGGAAAGAAAAAGTTCTATACGGCAATAGTGACAAATGTACATT
ATGCGGCCCCGGAGGGGTATGAAACGAAGGATATAGCTGAAGTGCTGGATTCATCTCCGGTACTGTTACCGAAACAATAT
GAGTTCTGGCAATGGCTGGCGGAATATTATCTTTGTACATTAGGAGATGTGTACAAGGCGGCTATCCCTTCGGGTATGAA
ACTGGAAAGCGAAACGTTGGTGGAATATAATCCCGATTTTGAAGCCACTGCACCCTTACCGGAGAAAGAGCAGAAAATTT
TGGATTTGTTGAGCCGGGATACAGAACAGTGTGTAACCCAGTTGGAAAAAAACAGCGGACTGAAAAATGTGTTGACTGTC
ATCAAGTCTTTGTTGGACAAAGAGGCCATCTTTGTCAAGGAGGAACTGAAACGTAATTACAAACCGCGTACAGAGGCAAG
GGTGAGGTTGGTGAACGGAGAAGCGGATGAGGCTTACTTACAACGGCTGTTCAATGAATTGTCACGTGCTCCTAAACAAT
TGATGATATTAATGAAGTATGTGGAATTGTCCGGTTGGGTAGCCAGAGGGTATGCTTTAAAGGAAGTAACAAAGAAAGAG
CTGCTTGAAAAGTCTGGAGGGTCTGCGGCCGTTTTTAACGGATTGGTCGAAAAGAAAGTCTTTGAGGTCTATCATCAGGA
GATAGGACGTTTGGATAAAGGAATTTCGGATACCGGTGATATTAATCCGCTGAATATTGCGCAGCAACAGGCTTATGGCA
ACATTCTTCAATGCTTCAGAGAGAAAAATGTGTGTCTTCTGCATGGCGTTACATCCAGTGGCAAGACGGAAATTTATATC
CACCTCATTCAGGAGGTGCTGAAAACAGGAAAGCAGGTCTTATATTTGCTTCCGGAAATTGCACTGACCACTCAGATAAC
TGAGCGTTTGAAAAGGGTGTTCGGACATCGGCTGGGTATTTATCATTCCAAGTTTCCGGACGCTGAGCGGGTGGAGATAT
GGCAGAAACAGTTGGGAGAGAAGAGCTATGATGTGATTCTCGGAGTCCGTTCCTCTATTTTTCTGCCTTTTCGTAATTTG
GGGCTGGTCATCATAGATGAGGAACACGAGAATACGTATAAACAGCAAGACCCCGCCCCACGTTACCATGCCCGTAGCTC
AGCCATCATGCTGGCCTCTATGTTCAAGGCGAAAGTGCTGCTGGGGACGGCTACTCCTGGTGTGGAAACTTACTTTAATG
CTACCTCGGGAAAGTATGGCTTGGTAGAATTGAAAGAAAGATATAAAGATATCCGGCTTCCTCATATAGAACTGGTTGAC
ATCAAGGAACTGGCGCATCAGAAGCGGATGCAGGGCCCTTTCTCACCGGCGCTGGTCAAGCAGATAAAAGAGGCATTGGA
GTGTAAGGAGCAGGTTATCCTGTTTCAGAACCGTCGCGGTTTCGCTCCGATGATAGAATGTCATACCTGCGGCTGGGTTC
CTAAATGCAAGAACTGTGATGTGAGTCTGACGTATCATAAGGGGCTGAACCAGCTGACTTGCCATTACTGCGGATATACC
TATCAAGTTCCCCGTTCCTGCCCGGCGTGCGGCGGAGTGGAATTGATGCATCGCGGTTTCGGTACAGAGCGTATAGAAGA
TGATATAAAACTGATATTTCCCGAGGCGAAAGTCGCCCGGATGGATTTGGATACCACTCGTACCCGTACCGCATACGAGA
AAATTATCGCGGATTTTGAACAGGGCAAGACGGACATCCTGATTGGTACGCAGATGGTGTCCAAAGGATTGGACTTTGAT
CATGTCAGTGTGGTGGGGATTCTGAATGCGGATACGATGTTGAACTTCCCGGATTTCCGGAGCTATGAGCGTGCTTTCCA
ACTGATGGCCCAAGTAGCAGGACGTGCCGGGCGTAAAAATAAGCAAGGACTGGTTATCCTTCAGACCAAATCGCCCGATT
TACCCGTTATTCATCAAGTGATGCATAATGACTATGAGCAGTTATATTATGACCAATTGGCAGAACGGCAGATGTTCAAA
TATCCTCCTTATTATCGTTTGATTTATGTATATCTGAAACACCGCAAAGAGGATATTCTGGATTTGGCGGCGGATACCAT
GGCGGCACAACTCCGTTCCGGATTGGGCGACAGGGTATTGGGTCCGGACAAACCGCCGGTGGCCCGTATCCAGACCTTAT
TTATAAAAAAAATGATTGTGAAAGTGGAGCAGAATGCTTCTATAAAGAAAGTGCGTGATTATCTGCTTGCCGTACAACGT
GCCATATTGGAAGATGAACGTTTCCGTTCGTTGTTAGTATATTATGATGTAGATCCACAGTAA

Upstream 100 bases:

>100_bases
GCAAGTTTCTTTGGCATACTTGTTCTAGACAACCTTATTTAATAAAAGATAAAAAGGAGGCATTCAGTGAAGAATGCCTC
CTTTTTTGTAATTTTGCACC

Downstream 100 bases:

>100_bases
CCGGTCCATGCACTGGACGATAATAGAAAAGATAAACGAAAATAAAGATGAAGATAGAATTAGACGTACATACCCATACC
ATTGCCAGCGGGCATGCATT

Product: putative primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 820; Mature: 820

Protein sequence:

>820_residues
MKKFVDVIVPLPIANQYTYSLPQEMEEMVQIGCRVVVPFGKKKFYTAIVTNVHYAAPEGYETKDIAEVLDSSPVLLPKQY
EFWQWLAEYYLCTLGDVYKAAIPSGMKLESETLVEYNPDFEATAPLPEKEQKILDLLSRDTEQCVTQLEKNSGLKNVLTV
IKSLLDKEAIFVKEELKRNYKPRTEARVRLVNGEADEAYLQRLFNELSRAPKQLMILMKYVELSGWVARGYALKEVTKKE
LLEKSGGSAAVFNGLVEKKVFEVYHQEIGRLDKGISDTGDINPLNIAQQQAYGNILQCFREKNVCLLHGVTSSGKTEIYI
HLIQEVLKTGKQVLYLLPEIALTTQITERLKRVFGHRLGIYHSKFPDAERVEIWQKQLGEKSYDVILGVRSSIFLPFRNL
GLVIIDEEHENTYKQQDPAPRYHARSSAIMLASMFKAKVLLGTATPGVETYFNATSGKYGLVELKERYKDIRLPHIELVD
IKELAHQKRMQGPFSPALVKQIKEALECKEQVILFQNRRGFAPMIECHTCGWVPKCKNCDVSLTYHKGLNQLTCHYCGYT
YQVPRSCPACGGVELMHRGFGTERIEDDIKLIFPEAKVARMDLDTTRTRTAYEKIIADFEQGKTDILIGTQMVSKGLDFD
HVSVVGILNADTMLNFPDFRSYERAFQLMAQVAGRAGRKNKQGLVILQTKSPDLPVIHQVMHNDYEQLYYDQLAERQMFK
YPPYYRLIYVYLKHRKEDILDLAADTMAAQLRSGLGDRVLGPDKPPVARIQTLFIKKMIVKVEQNASIKKVRDYLLAVQR
AILEDERFRSLLVYYDVDPQ

Sequences:

>Translated_820_residues
MKKFVDVIVPLPIANQYTYSLPQEMEEMVQIGCRVVVPFGKKKFYTAIVTNVHYAAPEGYETKDIAEVLDSSPVLLPKQY
EFWQWLAEYYLCTLGDVYKAAIPSGMKLESETLVEYNPDFEATAPLPEKEQKILDLLSRDTEQCVTQLEKNSGLKNVLTV
IKSLLDKEAIFVKEELKRNYKPRTEARVRLVNGEADEAYLQRLFNELSRAPKQLMILMKYVELSGWVARGYALKEVTKKE
LLEKSGGSAAVFNGLVEKKVFEVYHQEIGRLDKGISDTGDINPLNIAQQQAYGNILQCFREKNVCLLHGVTSSGKTEIYI
HLIQEVLKTGKQVLYLLPEIALTTQITERLKRVFGHRLGIYHSKFPDAERVEIWQKQLGEKSYDVILGVRSSIFLPFRNL
GLVIIDEEHENTYKQQDPAPRYHARSSAIMLASMFKAKVLLGTATPGVETYFNATSGKYGLVELKERYKDIRLPHIELVD
IKELAHQKRMQGPFSPALVKQIKEALECKEQVILFQNRRGFAPMIECHTCGWVPKCKNCDVSLTYHKGLNQLTCHYCGYT
YQVPRSCPACGGVELMHRGFGTERIEDDIKLIFPEAKVARMDLDTTRTRTAYEKIIADFEQGKTDILIGTQMVSKGLDFD
HVSVVGILNADTMLNFPDFRSYERAFQLMAQVAGRAGRKNKQGLVILQTKSPDLPVIHQVMHNDYEQLYYDQLAERQMFK
YPPYYRLIYVYLKHRKEDILDLAADTMAAQLRSGLGDRVLGPDKPPVARIQTLFIKKMIVKVEQNASIKKVRDYLLAVQR
AILEDERFRSLLVYYDVDPQ
>Mature_820_residues
MKKFVDVIVPLPIANQYTYSLPQEMEEMVQIGCRVVVPFGKKKFYTAIVTNVHYAAPEGYETKDIAEVLDSSPVLLPKQY
EFWQWLAEYYLCTLGDVYKAAIPSGMKLESETLVEYNPDFEATAPLPEKEQKILDLLSRDTEQCVTQLEKNSGLKNVLTV
IKSLLDKEAIFVKEELKRNYKPRTEARVRLVNGEADEAYLQRLFNELSRAPKQLMILMKYVELSGWVARGYALKEVTKKE
LLEKSGGSAAVFNGLVEKKVFEVYHQEIGRLDKGISDTGDINPLNIAQQQAYGNILQCFREKNVCLLHGVTSSGKTEIYI
HLIQEVLKTGKQVLYLLPEIALTTQITERLKRVFGHRLGIYHSKFPDAERVEIWQKQLGEKSYDVILGVRSSIFLPFRNL
GLVIIDEEHENTYKQQDPAPRYHARSSAIMLASMFKAKVLLGTATPGVETYFNATSGKYGLVELKERYKDIRLPHIELVD
IKELAHQKRMQGPFSPALVKQIKEALECKEQVILFQNRRGFAPMIECHTCGWVPKCKNCDVSLTYHKGLNQLTCHYCGYT
YQVPRSCPACGGVELMHRGFGTERIEDDIKLIFPEAKVARMDLDTTRTRTAYEKIIADFEQGKTDILIGTQMVSKGLDFD
HVSVVGILNADTMLNFPDFRSYERAFQLMAQVAGRAGRKNKQGLVILQTKSPDLPVIHQVMHNDYEQLYYDQLAERQMFK
YPPYYRLIYVYLKHRKEDILDLAADTMAAQLRSGLGDRVLGPDKPPVARIQTLFIKKMIVKVEQNASIKKVRDYLLAVQR
AILEDERFRSLLVYYDVDPQ

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=551, Percent_Identity=37.7495462794918, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 93871; Mature: 93871

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFVDVIVPLPIANQYTYSLPQEMEEMVQIGCRVVVPFGKKKFYTAIVTNVHYAAPEGY
CCCHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCEECCCCCC
ETKDIAEVLDSSPVLLPKQYEFWQWLAEYYLCTLGDVYKAAIPSGMKLESETLVEYNPDF
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEECCCC
EATAPLPEKEQKILDLLSRDTEQCVTQLEKNSGLKNVLTVIKSLLDKEAIFVKEELKRNY
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPRTEARVRLVNGEADEAYLQRLFNELSRAPKQLMILMKYVELSGWVARGYALKEVTKKE
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHCCHHHHHHHHHH
LLEKSGGSAAVFNGLVEKKVFEVYHQEIGRLDKGISDTGDINPLNIAQQQAYGNILQCFR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EKNVCLLHGVTSSGKTEIYIHLIQEVLKTGKQVLYLLPEIALTTQITERLKRVFGHRLGI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YHSKFPDAERVEIWQKQLGEKSYDVILGVRSSIFLPFRNLGLVIIDEEHENTYKQQDPAP
CCCCCCCHHHHHHHHHHHCCCCHHEEEECHHHHEEEHHCCCEEEEECCCCCHHHCCCCCC
RYHARSSAIMLASMFKAKVLLGTATPGVETYFNATSGKYGLVELKERYKDIRLPHIELVD
CHHCCHHHHHHHHHHHHHHEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEC
IKELAHQKRMQGPFSPALVKQIKEALECKEQVILFQNRRGFAPMIECHTCGWVPKCKNCD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCCCCCCCC
VSLTYHKGLNQLTCHYCGYTYQVPRSCPACGGVELMHRGFGTERIEDDIKLIFPEAKVAR
EEEEHHCCCCHHHHHHCCEEEECCCCCCCCCCHHHHHCCCCCHHHCCCCEEECCCCHHHH
MDLDTTRTRTAYEKIIADFEQGKTDILIGTQMVSKGLDFDHVSVVGILNADTMLNFPDFR
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCHH
SYERAFQLMAQVAGRAGRKNKQGLVILQTKSPDLPVIHQVMHNDYEQLYYDQLAERQMFK
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHC
YPPYYRLIYVYLKHRKEDILDLAADTMAAQLRSGLGDRVLGPDKPPVARIQTLFIKKMIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
KVEQNASIKKVRDYLLAVQRAILEDERFRSLLVYYDVDPQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCC
>Mature Secondary Structure
MKKFVDVIVPLPIANQYTYSLPQEMEEMVQIGCRVVVPFGKKKFYTAIVTNVHYAAPEGY
CCCHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCEECCCCCC
ETKDIAEVLDSSPVLLPKQYEFWQWLAEYYLCTLGDVYKAAIPSGMKLESETLVEYNPDF
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEECCCC
EATAPLPEKEQKILDLLSRDTEQCVTQLEKNSGLKNVLTVIKSLLDKEAIFVKEELKRNY
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPRTEARVRLVNGEADEAYLQRLFNELSRAPKQLMILMKYVELSGWVARGYALKEVTKKE
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHCCHHHHHHHHHH
LLEKSGGSAAVFNGLVEKKVFEVYHQEIGRLDKGISDTGDINPLNIAQQQAYGNILQCFR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EKNVCLLHGVTSSGKTEIYIHLIQEVLKTGKQVLYLLPEIALTTQITERLKRVFGHRLGI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YHSKFPDAERVEIWQKQLGEKSYDVILGVRSSIFLPFRNLGLVIIDEEHENTYKQQDPAP
CCCCCCCHHHHHHHHHHHCCCCHHEEEECHHHHEEEHHCCCEEEEECCCCCHHHCCCCCC
RYHARSSAIMLASMFKAKVLLGTATPGVETYFNATSGKYGLVELKERYKDIRLPHIELVD
CHHCCHHHHHHHHHHHHHHEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEC
IKELAHQKRMQGPFSPALVKQIKEALECKEQVILFQNRRGFAPMIECHTCGWVPKCKNCD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCCCCCCCC
VSLTYHKGLNQLTCHYCGYTYQVPRSCPACGGVELMHRGFGTERIEDDIKLIFPEAKVAR
EEEEHHCCCCHHHHHHCCEEEECCCCCCCCCCHHHHHCCCCCHHHCCCCEEECCCCHHHH
MDLDTTRTRTAYEKIIADFEQGKTDILIGTQMVSKGLDFDHVSVVGILNADTMLNFPDFR
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCHH
SYERAFQLMAQVAGRAGRKNKQGLVILQTKSPDLPVIHQVMHNDYEQLYYDQLAERQMFK
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHC
YPPYYRLIYVYLKHRKEDILDLAADTMAAQLRSGLGDRVLGPDKPPVARIQTLFIKKMIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
KVEQNASIKKVRDYLLAVQRAILEDERFRSLLVYYDVDPQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]