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The map label for this gene is dut

Identifier: 150003975

GI number: 150003975

Start: 1884131

End: 1884565

Strand: Direct

Name: dut

Synonym: BVU_1409

Alternate gene names: 150003975

Gene position: 1884131-1884565 (Clockwise)

Preceding gene: 150003973

Following gene: 150003976

Centisome position: 36.49

GC content: 46.9

Gene sequence:

>435_bases
ATGAAAATACAGGTAATCAATAAATCAAAACATGCTTTACCCGAGTATGCTACCGGGCAATCGGCAGGGATGGATATCCG
TGCTAATCTTGACGAACCGATTGTGCTGAAACCCTTGCAACGTTGTTTGGTTCCTACGGGACTTTATATCGCATTGCCTG
AAGGTTTTGAAGCGCAAATCCGTCCACGTAGCGGGCTGGCTATTAAGAAAGGCATCGGAGTCCTGAATTCTCCGGGCACA
ATTGATGCCGATTATCGTGGTGAAATTTGTATTATCCTGGTCAATTTGTCATCTGAGGATTTTATGATAGAAGATGGGGA
GCGCATTGCGCAAATGGTTGTAGCCCGTCACGAACATGCCGAATGGCAGGAAGTGGAGGTGCTGGACGAAACGGAACGTG
GTGCAGGTGGCTTTGGACATACCGGAAAAAAGTAA

Upstream 100 bases:

>100_bases
CGTCAATATGCCAATGGGCTGTATCATGTCTGGACTGTGTTTGTTGTCTGCAAATTATTAGCACATTGCCATATAAGCAT
ATTGATAAATTATTAAAATT

Downstream 100 bases:

>100_bases
AAGGAAACAATGAATAGAAGATTGAAATATGTGCCCATGCTATGTGTATGTCTGATAGGCATGCTTGTTTCATGCGGTAC
GGTGAAGAGAGCTTCCGGCT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MKIQVINKSKHALPEYATGQSAGMDIRANLDEPIVLKPLQRCLVPTGLYIALPEGFEAQIRPRSGLAIKKGIGVLNSPGT
IDADYRGEICIILVNLSSEDFMIEDGERIAQMVVARHEHAEWQEVEVLDETERGAGGFGHTGKK

Sequences:

>Translated_144_residues
MKIQVINKSKHALPEYATGQSAGMDIRANLDEPIVLKPLQRCLVPTGLYIALPEGFEAQIRPRSGLAIKKGIGVLNSPGT
IDADYRGEICIILVNLSSEDFMIEDGERIAQMVVARHEHAEWQEVEVLDETERGAGGFGHTGKK
>Mature_144_residues
MKIQVINKSKHALPEYATGQSAGMDIRANLDEPIVLKPLQRCLVPTGLYIALPEGFEAQIRPRSGLAIKKGIGVLNSPGT
IDADYRGEICIILVNLSSEDFMIEDGERIAQMVVARHEHAEWQEVEVLDETERGAGGFGHTGKK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=41.3793103448276, Blast_Score=112, Evalue=1e-25,
Organism=Homo sapiens, GI4503423, Length=144, Percent_Identity=40.9722222222222, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI70906441, Length=144, Percent_Identity=40.9722222222222, Blast_Score=111, Evalue=3e-25,
Organism=Escherichia coli, GI1790071, Length=133, Percent_Identity=46.6165413533835, Blast_Score=115, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71988561, Length=144, Percent_Identity=39.5833333333333, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319729, Length=144, Percent_Identity=37.5, Blast_Score=97, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19921126, Length=131, Percent_Identity=38.9312977099237, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583610, Length=131, Percent_Identity=38.9312977099237, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_BACV8 (A6L083)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298719.1
- ProteinModelPortal:   A6L083
- SMR:   A6L083
- STRING:   A6L083
- GeneID:   5302375
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1409
- NMPDR:   fig|435590.6.peg.1331
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- ProtClustDB:   PRK00601
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15779; Mature: 15779

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: BINDING 76-76

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQVINKSKHALPEYATGQSAGMDIRANLDEPIVLKPLQRCLVPTGLYIALPEGFEAQI
CEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCEEE
RPRSGLAIKKGIGVLNSPGTIDADYRGEICIILVNLSSEDFMIEDGERIAQMVVARHEHA
CCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHCCCC
EWQEVEVLDETERGAGGFGHTGKK
CCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIQVINKSKHALPEYATGQSAGMDIRANLDEPIVLKPLQRCLVPTGLYIALPEGFEAQI
CEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCEEE
RPRSGLAIKKGIGVLNSPGTIDADYRGEICIILVNLSSEDFMIEDGERIAQMVVARHEHA
CCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHCCCC
EWQEVEVLDETERGAGGFGHTGKK
CCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA