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The map label for this gene is topA [H]

Identifier: 150003902

GI number: 150003902

Start: 1784648

End: 1787086

Strand: Direct

Name: topA [H]

Synonym: BVU_1335

Alternate gene names: 150003902

Gene position: 1784648-1787086 (Clockwise)

Preceding gene: 150003901

Following gene: 150003903

Centisome position: 34.56

GC content: 46.08

Gene sequence:

>2439_bases
ATGCAAAAAAATCTAGTAATAGTTGAGTCACCCGCCAAGGCTAAAACCATTGAGAAATTCTTGGGCAATGATTATAAAGT
CCTTTCCAGTTACGGGCATATCCGGGATTTGAAAAAGAAGGAGTTTAGTATCGATGTAGAGAATGGGTTTGAGCCGACTT
ACGAGATACCGGCAGATAAAAAGAAATTGGTAGCCGAATTGAAAGCGGAGGCAAAGAAAGCTGATATGGTATGGCTTGCA
TCCGATGAGGACCGTGAAGGAGAAGCTATTTCATGGCACTTGTTTGAAGTCTTGGGGCTGGACCCTGAGAAGACCAAGCG
CATTGTATTCCACGAGATAACCAAAACAGCGATACTAAAGGCCATCGAAAATCCGCGTGACATTGATGTGAATCTGGTAA
ATGCCCAGCAGGCACGCCGTATTCTGGATAGAATTGTAGGTTTTGAACTCTCACCGGTATTGTGGAAGAAGGTAAAGCCT
GCTCTTTCTGCCGGACGTGTGCAATCTGTTGCCGTTCGTTTGATTGTGGAACGTGAACGTGAGGTGCATGCTTTTGTCAG
TGAGCCGTCTTATCGCGTTACAGCAGTGTTTGAAGTACCTGATGTCGATGGAAATGAAGTTGAAGTGAAAGCGGAATTGT
CCAACCGTTTCAAGACCAAGGAAGAAGCACAGGCTTTCCTTGAAACTTGTAAGGATGCGCAGTTCTCTATTGAAGATATA
ACAACCCGCCCTGTGAAGAAATCGCCGGCGGCACCGTTTACTACTTCTACGTTGCAGCAGGAAGCTGCCCGTAAGTTGGG
ATTTACAGTAGCGCAGACCATGATGGTGGCACAGCGTCTGTATGAAAACGGACAAATTACCTATATGCGTACGGATTCGG
TGAACCTTTCGGACCTGGCTTTGAATACCAGCAAGCAGACTATCTTGTCCCTGATGGGAGAAAGATACGTCAAAGTCCGT
AAGTTTGCCACCAAGACTAAAGGAGCACAGGAAGCGCATGAGGCTATCCGTCCTACCTATATGGAGAATGAAACGGTGAA
CGGAACCGGTCAGGAACAGAAATTGTATGAACTGATCTGGAAACGTACCATCGCTTCACAGATGGCAGATGCGGAGCTGG
AGAAAACAACGGCTACTATTGTAATCAGCAACAGTAGCGAGAAATTTATCGCTACCGGCGAGGTGATTACTTTTGACGGT
TTCCTGCGTGTATACAAGGAATCATACGATGATGACAATGAGCAGGAAGACGAGGGCCGTCTGTTGCCGCCTCTGAGCAA
AGGGGAAAAACTGATAAGAAAAGAAATCCTGGCGACTCAGCGCTTTACCCAATGTCCGCCCCGTTATACGGAAGCCAGCC
TGGTACGCAAACTGGAAGAGTTGGGCATCGGTCGTCCATCTACATATGCACCTACCATTTCTACTGTGCAGCAACGCGGT
TATGTAGAAAAAGGAAACAGTGAAGGTGTGAAACGCCCGTATGATATCTTGAAACTGAAAGGCGGCAAGATTACGGAAAC
TACCAAGACAGAGATGACGGGAAATGAAAAGGCCAAGTTGTTGCCTACTGATACCGGTATCGTGGTAAACGATTTCCTGA
TGGAATACTTTCCTGAAATCATGGATTTTAACTTTACAGCGAATGTGGAGAAAGAGTTCGATGAAGTGGCGGAAGGAGAA
AAGGAATGGACTGGCATGATGGACAGTTTCTATAAAGGCTTCCATCCGTTGGTAGATAAGACCATTCACTCTAAGACTGA
GCATAAGGTGGGTGAAAGGGTATTGGGAACAGATCCTGTAAGCGGTAAGCCGGTATCGGTTAAGATCGGTCGTTTCGGTC
CGGTTATTCAGATTGGTACTGCCGATGATGAGGAAAAACCGCGTTTTGCGCAGTTGGCAAAGGGATTGTCGATGGAAACC
ATTACGTTGGAAGAAGCATTGGATGCGTTCAAGCTTCCCCGTACGTTGGGGGATTATGACGGCCATACGGTAACGGTAGG
AGTAGGGCGTTTCGGACCATACGTGCGCTATGACAAACTGTTTGTTTCTATTCCTAAAGGAACGGATCCGATGGAGATTT
CTTTGGATGAGGCGGTAGAGTTGATCAAGAATAAGATAGAAGCGCAGGAAAAGAAGTTTATTAAAGTATTCGATGCTGAC
CCTGATATGCAGGTCTTGAACGGACGTTACGGCCCATACATCAGTTATCAGAAAAAGAATTATAAGATTCCTGAGAATGT
AGAACCGGCAGATTTAAGTCTGGAGGCTTGTTTTAAGGTGATTGAATTGCAGAAAAGCAAGGCGGAAACGCGTAAAACGA
AAGCGGCTTCAAGAAACAGGGGAACCGTGAATATGGAAGAGGAGTCCGAAAAGCCCGAAGAATCCGCAAAAAGTAAGGCG
GCTTCCAAGGTAAAGGGCACTGCAAAAGCAAAAAAATGA

Upstream 100 bases:

>100_bases
ACTTTTTTAAGAAAAAAAAGCAGAAACATACCTTATTATATAGGTGTGCTTCTGCTTTTTTTCTACTTTTGCCCCAAAAT
AAAATGGAATACATAGTATT

Downstream 100 bases:

>100_bases
CACGTATATTTTTAATAGGATATATGGGCGCGGGCAAAACGACTCTAGGTAAGGCGTTTGCCCGTGAAATGAGTCTGAAT
TTCATTGATTTGGACTGGTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 812; Mature: 812

Protein sequence:

>812_residues
MQKNLVIVESPAKAKTIEKFLGNDYKVLSSYGHIRDLKKKEFSIDVENGFEPTYEIPADKKKLVAELKAEAKKADMVWLA
SDEDREGEAISWHLFEVLGLDPEKTKRIVFHEITKTAILKAIENPRDIDVNLVNAQQARRILDRIVGFELSPVLWKKVKP
ALSAGRVQSVAVRLIVEREREVHAFVSEPSYRVTAVFEVPDVDGNEVEVKAELSNRFKTKEEAQAFLETCKDAQFSIEDI
TTRPVKKSPAAPFTTSTLQQEAARKLGFTVAQTMMVAQRLYENGQITYMRTDSVNLSDLALNTSKQTILSLMGERYVKVR
KFATKTKGAQEAHEAIRPTYMENETVNGTGQEQKLYELIWKRTIASQMADAELEKTTATIVISNSSEKFIATGEVITFDG
FLRVYKESYDDDNEQEDEGRLLPPLSKGEKLIRKEILATQRFTQCPPRYTEASLVRKLEELGIGRPSTYAPTISTVQQRG
YVEKGNSEGVKRPYDILKLKGGKITETTKTEMTGNEKAKLLPTDTGIVVNDFLMEYFPEIMDFNFTANVEKEFDEVAEGE
KEWTGMMDSFYKGFHPLVDKTIHSKTEHKVGERVLGTDPVSGKPVSVKIGRFGPVIQIGTADDEEKPRFAQLAKGLSMET
ITLEEALDAFKLPRTLGDYDGHTVTVGVGRFGPYVRYDKLFVSIPKGTDPMEISLDEAVELIKNKIEAQEKKFIKVFDAD
PDMQVLNGRYGPYISYQKKNYKIPENVEPADLSLEACFKVIELQKSKAETRKTKAASRNRGTVNMEEESEKPEESAKSKA
ASKVKGTAKAKK

Sequences:

>Translated_812_residues
MQKNLVIVESPAKAKTIEKFLGNDYKVLSSYGHIRDLKKKEFSIDVENGFEPTYEIPADKKKLVAELKAEAKKADMVWLA
SDEDREGEAISWHLFEVLGLDPEKTKRIVFHEITKTAILKAIENPRDIDVNLVNAQQARRILDRIVGFELSPVLWKKVKP
ALSAGRVQSVAVRLIVEREREVHAFVSEPSYRVTAVFEVPDVDGNEVEVKAELSNRFKTKEEAQAFLETCKDAQFSIEDI
TTRPVKKSPAAPFTTSTLQQEAARKLGFTVAQTMMVAQRLYENGQITYMRTDSVNLSDLALNTSKQTILSLMGERYVKVR
KFATKTKGAQEAHEAIRPTYMENETVNGTGQEQKLYELIWKRTIASQMADAELEKTTATIVISNSSEKFIATGEVITFDG
FLRVYKESYDDDNEQEDEGRLLPPLSKGEKLIRKEILATQRFTQCPPRYTEASLVRKLEELGIGRPSTYAPTISTVQQRG
YVEKGNSEGVKRPYDILKLKGGKITETTKTEMTGNEKAKLLPTDTGIVVNDFLMEYFPEIMDFNFTANVEKEFDEVAEGE
KEWTGMMDSFYKGFHPLVDKTIHSKTEHKVGERVLGTDPVSGKPVSVKIGRFGPVIQIGTADDEEKPRFAQLAKGLSMET
ITLEEALDAFKLPRTLGDYDGHTVTVGVGRFGPYVRYDKLFVSIPKGTDPMEISLDEAVELIKNKIEAQEKKFIKVFDAD
PDMQVLNGRYGPYISYQKKNYKIPENVEPADLSLEACFKVIELQKSKAETRKTKAASRNRGTVNMEEESEKPEESAKSKA
ASKVKGTAKAKK
>Mature_812_residues
MQKNLVIVESPAKAKTIEKFLGNDYKVLSSYGHIRDLKKKEFSIDVENGFEPTYEIPADKKKLVAELKAEAKKADMVWLA
SDEDREGEAISWHLFEVLGLDPEKTKRIVFHEITKTAILKAIENPRDIDVNLVNAQQARRILDRIVGFELSPVLWKKVKP
ALSAGRVQSVAVRLIVEREREVHAFVSEPSYRVTAVFEVPDVDGNEVEVKAELSNRFKTKEEAQAFLETCKDAQFSIEDI
TTRPVKKSPAAPFTTSTLQQEAARKLGFTVAQTMMVAQRLYENGQITYMRTDSVNLSDLALNTSKQTILSLMGERYVKVR
KFATKTKGAQEAHEAIRPTYMENETVNGTGQEQKLYELIWKRTIASQMADAELEKTTATIVISNSSEKFIATGEVITFDG
FLRVYKESYDDDNEQEDEGRLLPPLSKGEKLIRKEILATQRFTQCPPRYTEASLVRKLEELGIGRPSTYAPTISTVQQRG
YVEKGNSEGVKRPYDILKLKGGKITETTKTEMTGNEKAKLLPTDTGIVVNDFLMEYFPEIMDFNFTANVEKEFDEVAEGE
KEWTGMMDSFYKGFHPLVDKTIHSKTEHKVGERVLGTDPVSGKPVSVKIGRFGPVIQIGTADDEEKPRFAQLAKGLSMET
ITLEEALDAFKLPRTLGDYDGHTVTVGVGRFGPYVRYDKLFVSIPKGTDPMEISLDEAVELIKNKIEAQEKKFIKVFDAD
PDMQVLNGRYGPYISYQKKNYKIPENVEPADLSLEACFKVIELQKSKAETRKTKAASRNRGTVNMEEESEKPEESAKSKA
ASKVKGTAKAKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=435, Percent_Identity=26.8965517241379, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1787529, Length=616, Percent_Identity=42.5324675324675, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1788061, Length=453, Percent_Identity=25.60706401766, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17555378, Length=568, Percent_Identity=24.8239436619718, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=491, Percent_Identity=26.0692464358452, Blast_Score=97, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323263, Length=481, Percent_Identity=25.3638253638254, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585251, Length=474, Percent_Identity=28.0590717299578, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24640096, Length=538, Percent_Identity=22.8624535315985, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 91399; Mature: 91399

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKNLVIVESPAKAKTIEKFLGNDYKVLSSYGHIRDLKKKEFSIDVENGFEPTYEIPADK
CCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCEECCCCH
KKLVAELKAEAKKADMVWLASDEDREGEAISWHLFEVLGLDPEKTKRIVFHEITKTAILK
HHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIENPRDIDVNLVNAQQARRILDRIVGFELSPVLWKKVKPALSAGRVQSVAVRLIVERER
HHCCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
EVHAFVSEPSYRVTAVFEVPDVDGNEVEVKAELSNRFKTKEEAQAFLETCKDAQFSIEDI
HHHHHHCCCCEEEEEEEECCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHCCCCEEHHHH
TTRPVKKSPAAPFTTSTLQQEAARKLGFTVAQTMMVAQRLYENGQITYMRTDSVNLSDLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHE
LNTSKQTILSLMGERYVKVRKFATKTKGAQEAHEAIRPTYMENETVNGTGQEQKLYELIW
ECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
KRTIASQMADAELEKTTATIVISNSSEKFIATGEVITFDGFLRVYKESYDDDNEQEDEGR
HHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCEEEHHHHHHHHHHHCCCCCCCCCCCC
LLPPLSKGEKLIRKEILATQRFTQCPPRYTEASLVRKLEELGIGRPSTYAPTISTVQQRG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
YVEKGNSEGVKRPYDILKLKGGKITETTKTEMTGNEKAKLLPTDTGIVVNDFLMEYFPEI
CCCCCCCCCCCCCCHHEEECCCEECCCCCHHCCCCCCCEEECCCCCHHHHHHHHHHHHHH
MDFNFTANVEKEFDEVAEGEKEWTGMMDSFYKGFHPLVDKTIHSKTEHKVGERVLGTDPV
HCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGKPVSVKIGRFGPVIQIGTADDEEKPRFAQLAKGLSMETITLEEALDAFKLPRTLGDYD
CCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHCCHHHCCCC
GHTVTVGVGRFGPYVRYDKLFVSIPKGTDPMEISLDEAVELIKNKIEAQEKKFIKVFDAD
CCEEEEECCCCCCCEEECEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHCCEEEEECCC
PDMQVLNGRYGPYISYQKKNYKIPENVEPADLSLEACFKVIELQKSKAETRKTKAASRNR
CCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTVNMEEESEKPEESAKSKAASKVKGTAKAKK
CCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQKNLVIVESPAKAKTIEKFLGNDYKVLSSYGHIRDLKKKEFSIDVENGFEPTYEIPADK
CCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCEECCCCH
KKLVAELKAEAKKADMVWLASDEDREGEAISWHLFEVLGLDPEKTKRIVFHEITKTAILK
HHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIENPRDIDVNLVNAQQARRILDRIVGFELSPVLWKKVKPALSAGRVQSVAVRLIVERER
HHCCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
EVHAFVSEPSYRVTAVFEVPDVDGNEVEVKAELSNRFKTKEEAQAFLETCKDAQFSIEDI
HHHHHHCCCCEEEEEEEECCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHCCCCEEHHHH
TTRPVKKSPAAPFTTSTLQQEAARKLGFTVAQTMMVAQRLYENGQITYMRTDSVNLSDLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHE
LNTSKQTILSLMGERYVKVRKFATKTKGAQEAHEAIRPTYMENETVNGTGQEQKLYELIW
ECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
KRTIASQMADAELEKTTATIVISNSSEKFIATGEVITFDGFLRVYKESYDDDNEQEDEGR
HHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCEEEHHHHHHHHHHHCCCCCCCCCCCC
LLPPLSKGEKLIRKEILATQRFTQCPPRYTEASLVRKLEELGIGRPSTYAPTISTVQQRG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
YVEKGNSEGVKRPYDILKLKGGKITETTKTEMTGNEKAKLLPTDTGIVVNDFLMEYFPEI
CCCCCCCCCCCCCCHHEEECCCEECCCCCHHCCCCCCCEEECCCCCHHHHHHHHHHHHHH
MDFNFTANVEKEFDEVAEGEKEWTGMMDSFYKGFHPLVDKTIHSKTEHKVGERVLGTDPV
HCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGKPVSVKIGRFGPVIQIGTADDEEKPRFAQLAKGLSMETITLEEALDAFKLPRTLGDYD
CCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHCCHHHCCCC
GHTVTVGVGRFGPYVRYDKLFVSIPKGTDPMEISLDEAVELIKNKIEAQEKKFIKVFDAD
CCEEEEECCCCCCCEEECEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHCCEEEEECCC
PDMQVLNGRYGPYISYQKKNYKIPENVEPADLSLEACFKVIELQKSKAETRKTKAASRNR
CCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTVNMEEESEKPEESAKSKAASKVKGTAKAKK
CCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]