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The map label for this gene is ruvB [H]

Identifier: 150003857

GI number: 150003857

Start: 1737020

End: 1738048

Strand: Direct

Name: ruvB [H]

Synonym: BVU_1289

Alternate gene names: 150003857

Gene position: 1737020-1738048 (Clockwise)

Preceding gene: 150003852

Following gene: 150003858

Centisome position: 33.64

GC content: 46.74

Gene sequence:

>1029_bases
ATGGAAGAAAATTTTGATATACGCGAGCAACAGCTCACAACTAAAGAACGCGATTTTGAGAATGCCTTACGTCCGCTGAA
TTTTGAAGATTTCAGCGGGCAGGATAAGGTAGTGGATAATCTGCGCATCTTTGTCAAGGCGGCGCGTCTGCGGGCCGAGG
CGTTAGACCATGTTCTTTTGCACGGACCTCCCGGCTTGGGCAAGACGACCTTAAGTAATATCATTGCCAATGAACTGGGA
GTAGGTTTTAAAGTGACCTCAGGACCGGTGCTGGATAAACCGGGTGATTTGGCGGGAGTACTGACCAGCCTGGAACCCAA
TGATGTGCTTTTTATTGATGAAATACATCGGTTGAGCCCTGTGGTAGAAGAATATCTGTATTCTGCCATGGAGGATTATC
GTATCGATATAATGATAGACAAGGGACCTTCGGCGCGTAGTATCCAGTTGGAGTTGAGCCCGTTTACTTTGGTTGGAGCC
ACTACCCGGAGTGGGTTGCTGACTGCGCCTTTGCGTGCCCGTTTTGGAATTAATCTTCATTTGGAGTATTATGACGATGA
TGTATTGACAAGCATTATCCGTCGTTCAGCTACTATATTGAATGTGCCTTGTGACGTGAAAGCGGCGGGTGAGATTGCTT
CGCGCAGCCGTGGAACGCCCCGTATTGCAAATGCGCTGTTGAGGCGCGTCCGTGATTTTGCCCAGGTGAAAGGTTCCGGA
AGGATTGATGTGGAAATTGCCCGGTTTGCTTTAGAAGCCTTGAACATTGACAGGTATGGACTGGATGAGATAGATAACAA
GATTTTATGTACTATTATAGATAAGTTTAAGGGAGGCCCTGTGGGGCTGACTACTATTGCAACCGCTTTGGGTGAAGACG
CCGGAACAATAGAGGAGGTTTATGAACCTTTTTTGATTAAAGAAGGTTTCTTAAAACGTACTCCGCGTGGACGTGAGGTC
ACGGAACTGGCCTATATGCACCTGGGACGGAGCATTTATAATAGTCAGAAGACATTATTTGATGATTAA

Upstream 100 bases:

>100_bases
ATGTTGAAAAGAAGGACCAGAGAGGGACTGAAAATGAATTCCTCCATCTTTCTTTCTATAATAATAATATCTTTTGTACC
TTTGTGTCATAAACAAAAAA

Downstream 100 bases:

>100_bases
AAGATGGCAGGATTAAAAGCATTAGCCAAGGAAACAGCCATATATGGGATGAGTAGTATCATCGGGCGGTTCCTGAATTA
TTTGTTAGTACCGATATATA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MEENFDIREQQLTTKERDFENALRPLNFEDFSGQDKVVDNLRIFVKAARLRAEALDHVLLHGPPGLGKTTLSNIIANELG
VGFKVTSGPVLDKPGDLAGVLTSLEPNDVLFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSIQLELSPFTLVGA
TTRSGLLTAPLRARFGINLHLEYYDDDVLTSIIRRSATILNVPCDVKAAGEIASRSRGTPRIANALLRRVRDFAQVKGSG
RIDVEIARFALEALNIDRYGLDEIDNKILCTIIDKFKGGPVGLTTIATALGEDAGTIEEVYEPFLIKEGFLKRTPRGREV
TELAYMHLGRSIYNSQKTLFDD

Sequences:

>Translated_342_residues
MEENFDIREQQLTTKERDFENALRPLNFEDFSGQDKVVDNLRIFVKAARLRAEALDHVLLHGPPGLGKTTLSNIIANELG
VGFKVTSGPVLDKPGDLAGVLTSLEPNDVLFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSIQLELSPFTLVGA
TTRSGLLTAPLRARFGINLHLEYYDDDVLTSIIRRSATILNVPCDVKAAGEIASRSRGTPRIANALLRRVRDFAQVKGSG
RIDVEIARFALEALNIDRYGLDEIDNKILCTIIDKFKGGPVGLTTIATALGEDAGTIEEVYEPFLIKEGFLKRTPRGREV
TELAYMHLGRSIYNSQKTLFDD
>Mature_342_residues
MEENFDIREQQLTTKERDFENALRPLNFEDFSGQDKVVDNLRIFVKAARLRAEALDHVLLHGPPGLGKTTLSNIIANELG
VGFKVTSGPVLDKPGDLAGVLTSLEPNDVLFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSIQLELSPFTLVGA
TTRSGLLTAPLRARFGINLHLEYYDDDVLTSIIRRSATILNVPCDVKAAGEIASRSRGTPRIANALLRRVRDFAQVKGSG
RIDVEIARFALEALNIDRYGLDEIDNKILCTIIDKFKGGPVGLTTIATALGEDAGTIEEVYEPFLIKEGFLKRTPRGREV
TELAYMHLGRSIYNSQKTLFDD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=317, Percent_Identity=59.3059936908517, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 38021; Mature: 38021

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENFDIREQQLTTKERDFENALRPLNFEDFSGQDKVVDNLRIFVKAARLRAEALDHVLL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
HGPPGLGKTTLSNIIANELGVGFKVTSGPVLDKPGDLAGVLTSLEPNDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHCCCCCCEEEHHHHHHHHH
VVEEYLYSAMEDYRIDIMIDKGPSARSIQLELSPFTLVGATTRSGLLTAPLRARFGINLH
HHHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCEEEEECCCCCCCEECCHHHHCCEEEE
LEYYDDDVLTSIIRRSATILNVPCDVKAAGEIASRSRGTPRIANALLRRVRDFAQVKGSG
EEEECHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
RIDVEIARFALEALNIDRYGLDEIDNKILCTIIDKFKGGPVGLTTIATALGEDAGTIEEV
CEEHHHHHHHHHHHCCCCCCHHHHCCCEEEEEHHHCCCCCCHHHHHHHHHCCCCCCHHHH
YEPFLIKEGFLKRTPRGREVTELAYMHLGRSIYNSQKTLFDD
HHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MEENFDIREQQLTTKERDFENALRPLNFEDFSGQDKVVDNLRIFVKAARLRAEALDHVLL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
HGPPGLGKTTLSNIIANELGVGFKVTSGPVLDKPGDLAGVLTSLEPNDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHCCCCCCEEEHHHHHHHHH
VVEEYLYSAMEDYRIDIMIDKGPSARSIQLELSPFTLVGATTRSGLLTAPLRARFGINLH
HHHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCEEEEECCCCCCCEECCHHHHCCEEEE
LEYYDDDVLTSIIRRSATILNVPCDVKAAGEIASRSRGTPRIANALLRRVRDFAQVKGSG
EEEECHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
RIDVEIARFALEALNIDRYGLDEIDNKILCTIIDKFKGGPVGLTTIATALGEDAGTIEEV
CEEHHHHHHHHHHHCCCCCCHHHHCCCEEEEEHHHCCCCCCHHHHHHHHHCCCCCCHHHH
YEPFLIKEGFLKRTPRGREVTELAYMHLGRSIYNSQKTLFDD
HHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]