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The map label for this gene is parE [H]

Identifier: 150003803

GI number: 150003803

Start: 1658424

End: 1660301

Strand: Direct

Name: parE [H]

Synonym: BVU_1235

Alternate gene names: 150003803

Gene position: 1658424-1660301 (Clockwise)

Preceding gene: 150003802

Following gene: 150003804

Centisome position: 32.12

GC content: 45.15

Gene sequence:

>1878_bases
ATGGAAGAAAACAACGAACTGATAAACAATCCCGCCGTAGAATATACCGACGATAATATCCGCCACCTCAGTGACATGGA
GCATGTGCGTACCCGTCCCGGTATGTATATTGGAAAGTTGGGTGACGGATCACATGCCGAGGACGGTATCTACGTTCTTT
TGAAAGAAATTATCGATAACAGTATCGATGAATTTAAAATGCAGGCGGGTAAAAAGATTGAGATTATTATTGAAGAAAAT
CTCCGTGTCAGCGTGCGTGATTATGGCCGGGGTATTCCGCAGGGAAAACTCATTGAAGCTGTTAGTGTGTTGAATACGGG
AGGTAAATATGACAGCAAGGCGTTCAAGAAGAGCGTCGGACTGAATGGGGTCGGTGTGAAAGCCGTCAATGCATTGAGCT
CACGTTTCGAGGTGCGCAGCTATCGTGATGGAAAGGTGCGTATCGCTACCTTTGCCAAGGGAGACTTGTTGACGGATACC
ACACAAGATACAACAGAAGAGAACGGTACTTATATTTTCTTTGAACCGGATAACCTGTTGTTTGAGAACTACAGTTTCCG
TCCGGAGTTCGTGGAAACCATGCTCCGCAACTATACCTATCTGAATACAGGACTGGCCATTATCTATAACGGTCAGCGTA
TCCTGTCCCGTAACGGGTTGGTGGACTTGCTTAACGACAACATGACGGCTGTGGGATTATATCCCATCATCCACTTGAAA
GGAGAAGATATCGAGATCGCTTTTACGCATACCGGCCAGTATGGTGAGGAGTATTACTCTTTTGTCAACGGACAGCACAC
CACGCAGGGAGGTACGCATCAAAGTGCTTTCAAAGAGCATATGGCCCGTACCATCAAGGAATATTTCAACAAGAATATGG
ACTATGCCGATATCCGTAACGGATTGGTAGCTGCCATTGCCGTTAATGTGGAGGAACCGTTGTTCGAAAGTCAGACTAAA
ATCAAGCTGGGCTCTACCAATATGGCTCCCGGTGGTCCTACCGTTAATAAGTTTGTAGGTGATTTTGTAAAGACTGAGGT
CGATAACTATCTGCACAAACATACGGATGTGGCCGATGTGATGTTGCAGAAGATTCAAGAATCGGAGAAAGAGCGTAAGG
CTATAGCCGGAGTGACCAAACTGGCACGTGAACGTGCCAAAAAGGCAAATCTTCATAACCGTAAGTTGCGTGACTGCCGT
TTTCACCTGAATGATGCCAAAGGAGACAAGAAGGAGGAAAGCTGCATTTTCATTACCGAGGGTGACTCGGCGAGCGGTTC
TATCACCAAGAGCCGTGATGTAAATACTCAGGCAGTATTCAGTTTGCGTGGTAAGCCGTTGAATTCTTACGGGCTGACCA
AGAAGATTGTATATGAGAATGAAGAGTTTAACTTGCTTCAGGCTGCTCTGAACATAGAGGACGGTATGGAAGGATTACGA
TATAACAAAGTGATTGTGGCAACAGATGCCGATGTGGACGGTATGCACATCCGTTTGTTGCTGATTACCTTCTTCCTGCA
ATTCTTCCCCGATTTGATAAAGAAAGGTCATGTATATATCCTTCAGACTCCTTTGTTCCGTGTCCGTAACCGCAAGAAAG
GAGTTTATGAAACGGTATATTGTTATTCGGAAGAAGAACGGATTGCCGCAATCGAACGGTTAAGCCCCAATCCCGAAATC
ACACGGTTCAAAGGTTTGGGAGAGATTTCACCCGATGAATTCAAGCACTTTATCGGCAAAGATATGCGTTTGGAGCAAGT
TTCCCTCCGCAAGACTGATCTGGTAAAGGAGTTACTGGAGTTCTACATGGGTAAGAACACGATGGAACGTCAGAATTTTA
TTATAGATAACCTTGTTGTAGAAGAAGATATTGCGTGA

Upstream 100 bases:

>100_bases
GGCCCAGTGGGAATATTTCAAGACCGAGCAGCATAAACGCCGTCGTGCTTATAAAAAGAGTATTCTGTAACAACGATATA
TAAAAATGTGATAAGTCAAT

Downstream 100 bases:

>100_bases
GTGAATCACTAAAAAATATGAGAAGAGCGATATTTCCGGGAACCTTTGATCCTTTTACCATCGGTCATTATTCGGTCGTG
AAGAGAGCGTTGACTTTTAT

Product: DNA topoisomerase IV subunit B

Products: Double stranded DNA [C]

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MEENNELINNPAVEYTDDNIRHLSDMEHVRTRPGMYIGKLGDGSHAEDGIYVLLKEIIDNSIDEFKMQAGKKIEIIIEEN
LRVSVRDYGRGIPQGKLIEAVSVLNTGGKYDSKAFKKSVGLNGVGVKAVNALSSRFEVRSYRDGKVRIATFAKGDLLTDT
TQDTTEENGTYIFFEPDNLLFENYSFRPEFVETMLRNYTYLNTGLAIIYNGQRILSRNGLVDLLNDNMTAVGLYPIIHLK
GEDIEIAFTHTGQYGEEYYSFVNGQHTTQGGTHQSAFKEHMARTIKEYFNKNMDYADIRNGLVAAIAVNVEEPLFESQTK
IKLGSTNMAPGGPTVNKFVGDFVKTEVDNYLHKHTDVADVMLQKIQESEKERKAIAGVTKLARERAKKANLHNRKLRDCR
FHLNDAKGDKKEESCIFITEGDSASGSITKSRDVNTQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGMEGLR
YNKVIVATDADVDGMHIRLLLITFFLQFFPDLIKKGHVYILQTPLFRVRNRKKGVYETVYCYSEEERIAAIERLSPNPEI
TRFKGLGEISPDEFKHFIGKDMRLEQVSLRKTDLVKELLEFYMGKNTMERQNFIIDNLVVEEDIA

Sequences:

>Translated_625_residues
MEENNELINNPAVEYTDDNIRHLSDMEHVRTRPGMYIGKLGDGSHAEDGIYVLLKEIIDNSIDEFKMQAGKKIEIIIEEN
LRVSVRDYGRGIPQGKLIEAVSVLNTGGKYDSKAFKKSVGLNGVGVKAVNALSSRFEVRSYRDGKVRIATFAKGDLLTDT
TQDTTEENGTYIFFEPDNLLFENYSFRPEFVETMLRNYTYLNTGLAIIYNGQRILSRNGLVDLLNDNMTAVGLYPIIHLK
GEDIEIAFTHTGQYGEEYYSFVNGQHTTQGGTHQSAFKEHMARTIKEYFNKNMDYADIRNGLVAAIAVNVEEPLFESQTK
IKLGSTNMAPGGPTVNKFVGDFVKTEVDNYLHKHTDVADVMLQKIQESEKERKAIAGVTKLARERAKKANLHNRKLRDCR
FHLNDAKGDKKEESCIFITEGDSASGSITKSRDVNTQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGMEGLR
YNKVIVATDADVDGMHIRLLLITFFLQFFPDLIKKGHVYILQTPLFRVRNRKKGVYETVYCYSEEERIAAIERLSPNPEI
TRFKGLGEISPDEFKHFIGKDMRLEQVSLRKTDLVKELLEFYMGKNTMERQNFIIDNLVVEEDIA
>Mature_625_residues
MEENNELINNPAVEYTDDNIRHLSDMEHVRTRPGMYIGKLGDGSHAEDGIYVLLKEIIDNSIDEFKMQAGKKIEIIIEEN
LRVSVRDYGRGIPQGKLIEAVSVLNTGGKYDSKAFKKSVGLNGVGVKAVNALSSRFEVRSYRDGKVRIATFAKGDLLTDT
TQDTTEENGTYIFFEPDNLLFENYSFRPEFVETMLRNYTYLNTGLAIIYNGQRILSRNGLVDLLNDNMTAVGLYPIIHLK
GEDIEIAFTHTGQYGEEYYSFVNGQHTTQGGTHQSAFKEHMARTIKEYFNKNMDYADIRNGLVAAIAVNVEEPLFESQTK
IKLGSTNMAPGGPTVNKFVGDFVKTEVDNYLHKHTDVADVMLQKIQESEKERKAIAGVTKLARERAKKANLHNRKLRDCR
FHLNDAKGDKKEESCIFITEGDSASGSITKSRDVNTQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGMEGLR
YNKVIVATDADVDGMHIRLLLITFFLQFFPDLIKKGHVYILQTPLFRVRNRKKGVYETVYCYSEEERIAAIERLSPNPEI
TRFKGLGEISPDEFKHFIGKDMRLEQVSLRKTDLVKELLEFYMGKNTMERQNFIIDNLVVEEDIA

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=633, Percent_Identity=26.5402843601896, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI19913406, Length=632, Percent_Identity=25.6329113924051, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI48994957, Length=560, Percent_Identity=32.6785714285714, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1789408, Length=646, Percent_Identity=29.8761609907121, Blast_Score=244, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17535065, Length=632, Percent_Identity=27.8481012658228, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=548, Percent_Identity=25.5474452554745, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324241, Length=586, Percent_Identity=24.7440273037543, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17136538, Length=621, Percent_Identity=26.8921095008052, Blast_Score=120, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.3 [C]

Molecular weight: Translated: 71019; Mature: 71019

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENNELINNPAVEYTDDNIRHLSDMEHVRTRPGMYIGKLGDGSHAEDGIYVLLKEIIDN
CCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCC
SIDEFKMQAGKKIEIIIEENLRVSVRDYGRGIPQGKLIEAVSVLNTGGKYDSKAFKKSVG
CHHHHHHHCCCEEEEEEECCCEEEEHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCC
LNGVGVKAVNALSSRFEVRSYRDGKVRIATFAKGDLLTDTTQDTTEENGTYIFFEPDNLL
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEECCCCCCCCCCCCEEEEECCCCEE
FENYSFRPEFVETMLRNYTYLNTGLAIIYNGQRILSRNGLVDLLNDNMTAVGLYPIIHLK
ECCCCCCHHHHHHHHHCCEEECCCEEEEECCCEEECCCCCEEECCCCCEEEEEEEEEEEC
GEDIEIAFTHTGQYGEEYYSFVNGQHTTQGGTHQSAFKEHMARTIKEYFNKNMDYADIRN
CCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GLVAAIAVNVEEPLFESQTKIKLGSTNMAPGGPTVNKFVGDFVKTEVDNYLHKHTDVADV
CEEEEEEECCCCCHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
MLQKIQESEKERKAIAGVTKLARERAKKANLHNRKLRDCRFHLNDAKGDKKEESCIFITE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCCCCCCCCCCEEEEEC
GDSASGSITKSRDVNTQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGMEGLR
CCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHCHHHHHCCCE
YNKVIVATDADVDGMHIRLLLITFFLQFFPDLIKKGHVYILQTPLFRVRNRKKGVYETVY
ECEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCEEEEE
CYSEEERIAAIERLSPNPEITRFKGLGEISPDEFKHFIGKDMRLEQVSLRKTDLVKELLE
EECCCHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
FYMGKNTMERQNFIIDNLVVEEDIA
HHHCCCCHHHHHHHHHHEEECCCCC
>Mature Secondary Structure
MEENNELINNPAVEYTDDNIRHLSDMEHVRTRPGMYIGKLGDGSHAEDGIYVLLKEIIDN
CCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCC
SIDEFKMQAGKKIEIIIEENLRVSVRDYGRGIPQGKLIEAVSVLNTGGKYDSKAFKKSVG
CHHHHHHHCCCEEEEEEECCCEEEEHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCC
LNGVGVKAVNALSSRFEVRSYRDGKVRIATFAKGDLLTDTTQDTTEENGTYIFFEPDNLL
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEECCCCCCCCCCCCEEEEECCCCEE
FENYSFRPEFVETMLRNYTYLNTGLAIIYNGQRILSRNGLVDLLNDNMTAVGLYPIIHLK
ECCCCCCHHHHHHHHHCCEEECCCEEEEECCCEEECCCCCEEECCCCCEEEEEEEEEEEC
GEDIEIAFTHTGQYGEEYYSFVNGQHTTQGGTHQSAFKEHMARTIKEYFNKNMDYADIRN
CCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GLVAAIAVNVEEPLFESQTKIKLGSTNMAPGGPTVNKFVGDFVKTEVDNYLHKHTDVADV
CEEEEEEECCCCCHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
MLQKIQESEKERKAIAGVTKLARERAKKANLHNRKLRDCRFHLNDAKGDKKEESCIFITE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCCCCCCCCCCEEEEEC
GDSASGSITKSRDVNTQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGMEGLR
CCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHCHHHHHCCCE
YNKVIVATDADVDGMHIRLLLITFFLQFFPDLIKKGHVYILQTPLFRVRNRKKGVYETVY
ECEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCEEEEE
CYSEEERIAAIERLSPNPEITRFKGLGEISPDEFKHFIGKDMRLEQVSLRKTDLVKELLE
EECCCHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
FYMGKNTMERQNFIIDNLVVEEDIA
HHHCCCCHHHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double stranded DNA; DNA [C]

Specific reaction: ATP dependent breakage followed by passage and rejoining of double stranded DNA Protein + DNA = Protein-DNA [C]

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434 [H]