Click here to switch to the map view.

The map label for this gene is mnmG

Identifier: 150003782

GI number: 150003782

Start: 1633849

End: 1635720

Strand: Reverse

Name: mnmG

Synonym: BVU_1214

Alternate gene names: 150003782

Gene position: 1635720-1633849 (Counterclockwise)

Preceding gene: 150003783

Following gene: 150003781

Centisome position: 31.68

GC content: 51.34

Gene sequence:

>1872_bases
ATGGATTTTAAGTACGACGTTATTGTAATAGGCGCCGGACACGCAGGCTGTGAAGCAGCCGCGGCCGCAGCCAACATGGG
TTCGAAGACCTGCCTGATTACCATGGATATGAACAAAATCGGGCAAATGAGTTGTAACCCTGCCGTAGGTGGCATCGCCA
AGGGACAAATCGTAAGAGAGATAGACGCATTAGGTGGCTACATGGGACTGGTTACAGACCGTACAGCCATACAGTTCCGT
ATGCTCAACCGCTCCAAAGGTCCCGCTATGTGGAGCCCCCGCGCCCAATGTGACCGCGGCAAGTTTATCTGGGCATGGCG
CGAGATTCTGGAGAATACTCCCAATCTGCACATTTGGCAAGATACCGTAGAAGAACTAATCGTGGAAAACGGCGAAGCGA
CTGGAGTCATGACTTGCTGGGGAGTGACTTTCCATGCTAAATGTATCGTGCTCACCGCAGGAACCTTCCTGAACGGGCTG
ATGCACATCGGGCATAAACAACTGGCAGGAGGACGCATGGCCGAACCTGCCTCCTACCATCTGACCGAATCAATCACCCG
CCACGGAATCACCGCCGGACGCATGAAGACCGGTACGCCTGTCCGCATAGACGGGCGCAGCGTACACTACGACCTGATGG
AAACACAGGACGGAGAAAACGACTTCCATCGCTTTTCGTTCATGAGCGAACCGCGCAAACTGAAACAACTGCAATGCTGG
ACCTGCTTTACCAACGAAGAGGTACACGAGATACTACGGAAAGGTCTGCCCGACTCTCCCCTTTTCAACGGACAAATCCA
AAGCATCGGACCGCGTTATTGTCCCAGTATAGAAACCAAAATCGTAACCTTCCCCGACAAGCCGCAACATCAGCTTTTCC
TGGAACCCGAAGGAGAAACCACACAGGAACTTTACCTGAACGGGTTCTCTTCTTCACTGCCCATGGACATACAGTTGGCG
GCATTAAAGAAAGTACCCGCATTTAAGGACTTGGTAGTCTATCGTCCGGGATACGCCATCGAATATGATTACTTCGACCC
CACCCAACTGAAACATACGTTGGAATCGAAAATAATCAAGAACCTGTTCCTTGCCGGACAAGTAAACGGAACTACCGGTT
ACGAAGAAGCAGGCGGACAGGGAATCATTGCCGGCATCAACGCACACATCAATTGCCACGGTGGCGAACCGTTCACACTG
GGACGGGACGAGGCCTACATCGGTGTCTTGATTGACGATCTGGTTACAAAAGGTGTGGACGAACCTTATCGTATGTTCAC
CTCCCGTGCCGAATACCGCATCCTGCTCCGACAAGACGATGCAGATATGCGCCTTACCGAACGCGCCTACAAGCTGGGAC
TGGTAAAACAAGACCGTTACGAACATCTTTGCAGCAAACGGGAAGCCGTGAACCAAATCATCGACTTTGCCAAAACCTTC
TCCATCAAAGCCGCATTAATCAATGATGCTTTGGAAAGTTTGGGTACTGCCCGTCTGACACACGGCTGCAAACTAATCGA
CCTGCTGAACCGTCCGCAGATTACCATCGAAAATATAGCCGGCCACATTCCGGCTTTCAAAGCCATGCTGGACCAAATTA
CCGACCGTAAAGAAGAAGTGATTGAAGCTGCCGAAGTGCTGATCAAATACCAGGGATACATTGACCGCGAACGAATGATC
GCCGACAAGATACATCGTCTGGAAGCCATCCGCATCAAAGGCAAGTTCGATTACAACTCATTGAATTCGCTCTCTACCGA
AGCCCGGCAGAAACTGATGAAGATAGATCCCGAAACTTTGGCCCAAGCCAGCCGCATACCGGGTATCTCTCCTAGCGACA
TCAATGTCTTACTAGTCCTGCTGGGACGGTGA

Upstream 100 bases:

>100_bases
AGAAACAGATTTCTGCCGCCACACATTCTTTTTAACCGTATTATCACCGCTTTACAGAGTAAAATTCGTAATTTTGCAAT
CGGTAAAAAGAAATAAATCA

Downstream 100 bases:

>100_bases
AACAAGGTATGTTTCACGTGAAACAAGTAATTTAATAGACTAACAATAAAATACTGGTTATGAGCAAAGAAACGCTTAAA
GCAAACTTAAGAGAAATTCC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MDFKYDVIVIGAGHAGCEAAAAAANMGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVREIDALGGYMGLVTDRTAIQFR
MLNRSKGPAMWSPRAQCDRGKFIWAWREILENTPNLHIWQDTVEELIVENGEATGVMTCWGVTFHAKCIVLTAGTFLNGL
MHIGHKQLAGGRMAEPASYHLTESITRHGITAGRMKTGTPVRIDGRSVHYDLMETQDGENDFHRFSFMSEPRKLKQLQCW
TCFTNEEVHEILRKGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKPQHQLFLEPEGETTQELYLNGFSSSLPMDIQLA
ALKKVPAFKDLVVYRPGYAIEYDYFDPTQLKHTLESKIIKNLFLAGQVNGTTGYEEAGGQGIIAGINAHINCHGGEPFTL
GRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADMRLTERAYKLGLVKQDRYEHLCSKREAVNQIIDFAKTF
SIKAALINDALESLGTARLTHGCKLIDLLNRPQITIENIAGHIPAFKAMLDQITDRKEEVIEAAEVLIKYQGYIDRERMI
ADKIHRLEAIRIKGKFDYNSLNSLSTEARQKLMKIDPETLAQASRIPGISPSDINVLLVLLGR

Sequences:

>Translated_623_residues
MDFKYDVIVIGAGHAGCEAAAAAANMGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVREIDALGGYMGLVTDRTAIQFR
MLNRSKGPAMWSPRAQCDRGKFIWAWREILENTPNLHIWQDTVEELIVENGEATGVMTCWGVTFHAKCIVLTAGTFLNGL
MHIGHKQLAGGRMAEPASYHLTESITRHGITAGRMKTGTPVRIDGRSVHYDLMETQDGENDFHRFSFMSEPRKLKQLQCW
TCFTNEEVHEILRKGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKPQHQLFLEPEGETTQELYLNGFSSSLPMDIQLA
ALKKVPAFKDLVVYRPGYAIEYDYFDPTQLKHTLESKIIKNLFLAGQVNGTTGYEEAGGQGIIAGINAHINCHGGEPFTL
GRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADMRLTERAYKLGLVKQDRYEHLCSKREAVNQIIDFAKTF
SIKAALINDALESLGTARLTHGCKLIDLLNRPQITIENIAGHIPAFKAMLDQITDRKEEVIEAAEVLIKYQGYIDRERMI
ADKIHRLEAIRIKGKFDYNSLNSLSTEARQKLMKIDPETLAQASRIPGISPSDINVLLVLLGR
>Mature_623_residues
MDFKYDVIVIGAGHAGCEAAAAAANMGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVREIDALGGYMGLVTDRTAIQFR
MLNRSKGPAMWSPRAQCDRGKFIWAWREILENTPNLHIWQDTVEELIVENGEATGVMTCWGVTFHAKCIVLTAGTFLNGL
MHIGHKQLAGGRMAEPASYHLTESITRHGITAGRMKTGTPVRIDGRSVHYDLMETQDGENDFHRFSFMSEPRKLKQLQCW
TCFTNEEVHEILRKGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKPQHQLFLEPEGETTQELYLNGFSSSLPMDIQLA
ALKKVPAFKDLVVYRPGYAIEYDYFDPTQLKHTLESKIIKNLFLAGQVNGTTGYEEAGGQGIIAGINAHINCHGGEPFTL
GRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADMRLTERAYKLGLVKQDRYEHLCSKREAVNQIIDFAKTF
SIKAALINDALESLGTARLTHGCKLIDLLNRPQITIENIAGHIPAFKAMLDQITDRKEEVIEAAEVLIKYQGYIDRERMI
ADKIHRLEAIRIKGKFDYNSLNSLSTEARQKLMKIDPETLAQASRIPGISPSDINVLLVLLGR

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=634, Percent_Identity=39.7476340694006, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI19882217, Length=659, Percent_Identity=38.6949924127466, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI183227703, Length=674, Percent_Identity=37.3887240356083, Blast_Score=414, Evalue=1e-115,
Organism=Escherichia coli, GI2367273, Length=625, Percent_Identity=46.56, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=37.258064516129, Blast_Score=412, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321202, Length=621, Percent_Identity=40.5797101449275, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24658174, Length=629, Percent_Identity=41.4944356120827, Blast_Score=431, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_BACV8 (A6KZP0)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298526.1
- ProteinModelPortal:   A6KZP0
- SMR:   A6KZP0
- STRING:   A6KZP0
- GeneID:   5302180
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1214
- NMPDR:   fig|435590.6.peg.1146
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69612; Mature: 69612

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 123-123 BINDING 178-178 BINDING 367-367

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKYDVIVIGAGHAGCEAAAAAANMGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVRE
CCCEEEEEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
IDALGGYMGLVTDRTAIQFRMLNRSKGPAMWSPRAQCDRGKFIWAWREILENTPNLHIWQ
HHHHCCHHHHEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEH
DTVEELIVENGEATGVMTCWGVTFHAKCIVLTAGTFLNGLMHIGHKQLAGGRMAEPASYH
HHHHHHHHCCCCCCEEEEEECCEEEEEEEEEECHHHHHHHHHHCHHHHCCCCCCCCCHHH
LTESITRHGITAGRMKTGTPVRIDGRSVHYDLMETQDGENDFHRFSFMSEPRKLKQLQCW
HHHHHHHCCCCCCCCCCCCEEEECCCEEEEEHHCCCCCCCHHHHHHHHCCCHHHHHEEEE
TCFTNEEVHEILRKGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKPQHQLFLEPEGET
EECCCHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCC
TQELYLNGFSSSLPMDIQLAALKKVPAFKDLVVYRPGYAIEYDYFDPTQLKHTLESKIIK
CHHHHEECCCCCCCCEEEHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHH
NLFLAGQVNGTTGYEEAGGQGIIAGINAHINCHGGEPFTLGRDEAYIGVLIDDLVTKGVD
HHHHEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCEECCCCCCEEEHHHHHHHHCCCC
EPYRMFTSRAEYRILLRQDDADMRLTERAYKLGLVKQDRYEHLCSKREAVNQIIDFAKTF
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIKAALINDALESLGTARLTHGCKLIDLLNRPQITIENIAGHIPAFKAMLDQITDRKEEV
HHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHH
IEAAEVLIKYQGYIDRERMIADKIHRLEAIRIKGKFDYNSLNSLSTEARQKLMKIDPETL
HHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHH
AQASRIPGISPSDINVLLVLLGR
HHHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MDFKYDVIVIGAGHAGCEAAAAAANMGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVRE
CCCEEEEEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
IDALGGYMGLVTDRTAIQFRMLNRSKGPAMWSPRAQCDRGKFIWAWREILENTPNLHIWQ
HHHHCCHHHHEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEH
DTVEELIVENGEATGVMTCWGVTFHAKCIVLTAGTFLNGLMHIGHKQLAGGRMAEPASYH
HHHHHHHHCCCCCCEEEEEECCEEEEEEEEEECHHHHHHHHHHCHHHHCCCCCCCCCHHH
LTESITRHGITAGRMKTGTPVRIDGRSVHYDLMETQDGENDFHRFSFMSEPRKLKQLQCW
HHHHHHHCCCCCCCCCCCCEEEECCCEEEEEHHCCCCCCCHHHHHHHHCCCHHHHHEEEE
TCFTNEEVHEILRKGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKPQHQLFLEPEGET
EECCCHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCC
TQELYLNGFSSSLPMDIQLAALKKVPAFKDLVVYRPGYAIEYDYFDPTQLKHTLESKIIK
CHHHHEECCCCCCCCEEEHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHH
NLFLAGQVNGTTGYEEAGGQGIIAGINAHINCHGGEPFTLGRDEAYIGVLIDDLVTKGVD
HHHHEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCEECCCCCCEEEHHHHHHHHCCCC
EPYRMFTSRAEYRILLRQDDADMRLTERAYKLGLVKQDRYEHLCSKREAVNQIIDFAKTF
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIKAALINDALESLGTARLTHGCKLIDLLNRPQITIENIAGHIPAFKAMLDQITDRKEEV
HHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHH
IEAAEVLIKYQGYIDRERMIADKIHRLEAIRIKGKFDYNSLNSLSTEARQKLMKIDPETL
HHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHH
AQASRIPGISPSDINVLLVLLGR
HHHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA