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The map label for this gene is tyrS [H]

Identifier: 150003760

GI number: 150003760

Start: 1604442

End: 1605734

Strand: Direct

Name: tyrS [H]

Synonym: BVU_1191

Alternate gene names: 150003760

Gene position: 1604442-1605734 (Clockwise)

Preceding gene: 150003759

Following gene: 150003763

Centisome position: 31.07

GC content: 46.87

Gene sequence:

>1293_bases
ATGAATTTTGTAGAAGAATTAACATGGCGTGGAATGGTGCACACAATGATGCCGGGCACCGAAGAGTTATTGGCTAAAGA
ACAAGTGACAGCTTATCTGGGTATTGACCCTACGGCTGATTCTCTGCATATCGGCCACTTGTGTGGTGTGATGATGCTAC
GTCATTTCCAGCGTTGCGGTCATAAGCCGTTGGCATTGGTGGGCGGCGCGACCGGTATGATCGGTGACCCTTCCGGCAAA
TCGCAGGAGCGCAATTTGCTGACCGAGGAAACATTGCGTCATAATGTGGCTTGCATCAAGAAACAGCTGGCTAAGTTCCT
TGACTTTGAGAGCGATGCTCCCAACAAAGCCGAATTGGTGAATAACTATGACTGGATGAAGGATTTTACTTTCCTGGACT
TTGCCCGTGAGGTAGGGAAGCACATTACAGTGAATTATATGATGGCTAAGGATTCTGTACAGAAACGTTTGAACGGTGAG
GCGCGTGACGGTTTGTCATTTACAGAATTTACTTACCAGTTGTTGCAGGGATACGATTTCTTGCATCTTTATGAAACAAA
GGGCTGCAAACTTCAGATGGGTGGTTCGGATCAATGGGGTAATATCACTACCGGGGCCGAGCTGATTCGTCGTACCAATG
GCGGTGAAGTGTTTGCATTGACTTGTCCGTTGATTACAAAGGCCGATGGTGGTAAGTTCGGTAAGACTGAGTCTGGTAAT
ATTTGGTTGGACCCCCGTTATACTTCTCCTTACAAGTTCTATCAGTTTTGGTTGAATGTAAGTGACGAGGATGCCGCACG
TTATATAAAGATTTTCACTTCGCTGAGTCAGGAAGAAGTGGAAGCATTGACAGCAGAACATGCTGAAGCTCCGCACTTGC
GTGTATTGCAGAAGCGTCTGGCTAAGGAAGTAACTGTGATGGTTCATTCGGAAGAAGATTACAATGCTGCTGTTGAGGCA
TCGGGCATCCTGTTTGGTAACGCCACTTCCGAAGCGTTGAAAAAGCTGGATGAAGACACGTTGCTGGCAGTGTTCGAAGG
TGTTCCTCAGTTTGAGGTGTCACGAGATGCATTGGCCGAAGGTGTGAAGGCAGTAGACTTGTTCGTTGATAATGCCGCTG
TTTTCGCTTCTAAAGGTGAGATGCGTAAGCTTGTACAGGGCGGTGGCGTTTCATTGAACAAGGAGAAATTAAGTGCCTTT
GACCAGGTGATTACGACTGCTGACCTGCTGGATGAAAAATACCTGCTGGTTCAGCGCGGAAAGAAGAACTATTATCTGGT
GATTGCCAAATAA

Upstream 100 bases:

>100_bases
AAAATCTTTTTTCGGCAATAAGAGTTGTTTCTTCAGATAAAGAGTCGTACTTTTGTAGCATTTAAAGTATAAATCAAACA
TTTAAATAGATAATCATTCG

Downstream 100 bases:

>100_bases
GAAAAAAGAGAACTTCTTTTATAAATAAAAAGGGGATATTCAGTAAATGTCCCTACAAACAAAAACCGGAAGAAGAAAAC
CTGTTGTTTCTCCTTCCGGT

Product: putative tyrosyl-tRNA synthetase 1

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNFVEELTWRGMVHTMMPGTEELLAKEQVTAYLGIDPTADSLHIGHLCGVMMLRHFQRCGHKPLALVGGATGMIGDPSGK
SQERNLLTEETLRHNVACIKKQLAKFLDFESDAPNKAELVNNYDWMKDFTFLDFAREVGKHITVNYMMAKDSVQKRLNGE
ARDGLSFTEFTYQLLQGYDFLHLYETKGCKLQMGGSDQWGNITTGAELIRRTNGGEVFALTCPLITKADGGKFGKTESGN
IWLDPRYTSPYKFYQFWLNVSDEDAARYIKIFTSLSQEEVEALTAEHAEAPHLRVLQKRLAKEVTVMVHSEEDYNAAVEA
SGILFGNATSEALKKLDEDTLLAVFEGVPQFEVSRDALAEGVKAVDLFVDNAAVFASKGEMRKLVQGGGVSLNKEKLSAF
DQVITTADLLDEKYLLVQRGKKNYYLVIAK

Sequences:

>Translated_430_residues
MNFVEELTWRGMVHTMMPGTEELLAKEQVTAYLGIDPTADSLHIGHLCGVMMLRHFQRCGHKPLALVGGATGMIGDPSGK
SQERNLLTEETLRHNVACIKKQLAKFLDFESDAPNKAELVNNYDWMKDFTFLDFAREVGKHITVNYMMAKDSVQKRLNGE
ARDGLSFTEFTYQLLQGYDFLHLYETKGCKLQMGGSDQWGNITTGAELIRRTNGGEVFALTCPLITKADGGKFGKTESGN
IWLDPRYTSPYKFYQFWLNVSDEDAARYIKIFTSLSQEEVEALTAEHAEAPHLRVLQKRLAKEVTVMVHSEEDYNAAVEA
SGILFGNATSEALKKLDEDTLLAVFEGVPQFEVSRDALAEGVKAVDLFVDNAAVFASKGEMRKLVQGGGVSLNKEKLSAF
DQVITTADLLDEKYLLVQRGKKNYYLVIAK
>Mature_430_residues
MNFVEELTWRGMVHTMMPGTEELLAKEQVTAYLGIDPTADSLHIGHLCGVMMLRHFQRCGHKPLALVGGATGMIGDPSGK
SQERNLLTEETLRHNVACIKKQLAKFLDFESDAPNKAELVNNYDWMKDFTFLDFAREVGKHITVNYMMAKDSVQKRLNGE
ARDGLSFTEFTYQLLQGYDFLHLYETKGCKLQMGGSDQWGNITTGAELIRRTNGGEVFALTCPLITKADGGKFGKTESGN
IWLDPRYTSPYKFYQFWLNVSDEDAARYIKIFTSLSQEEVEALTAEHAEAPHLRVLQKRLAKEVTVMVHSEEDYNAAVEA
SGILFGNATSEALKKLDEDTLLAVFEGVPQFEVSRDALAEGVKAVDLFVDNAAVFASKGEMRKLVQGGGVSLNKEKLSAF
DQVITTADLLDEKYLLVQRGKKNYYLVIAK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=411, Percent_Identity=35.7664233576642, Blast_Score=250, Evalue=1e-66,
Organism=Escherichia coli, GI1787925, Length=431, Percent_Identity=46.8677494199536, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25145802, Length=399, Percent_Identity=34.3358395989975, Blast_Score=218, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI25145805, Length=337, Percent_Identity=31.4540059347181, Blast_Score=159, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6325160, Length=449, Percent_Identity=35.8574610244989, Blast_Score=276, Evalue=3e-75,
Organism=Drosophila melanogaster, GI20130383, Length=440, Percent_Identity=39.0909090909091, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 48041; Mature: 48041

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVEELTWRGMVHTMMPGTEELLAKEQVTAYLGIDPTADSLHIGHLCGVMMLRHFQRCG
CCHHHHHHHCCHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
HKPLALVGGATGMIGDPSGKSQERNLLTEETLRHNVACIKKQLAKFLDFESDAPNKAELV
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
NNYDWMKDFTFLDFAREVGKHITVNYMMAKDSVQKRLNGEARDGLSFTEFTYQLLQGYDF
CCCHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE
LHLYETKGCKLQMGGSDQWGNITTGAELIRRTNGGEVFALTCPLITKADGGKFGKTESGN
EEEEECCCCEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCCCCCC
IWLDPRYTSPYKFYQFWLNVSDEDAARYIKIFTSLSQEEVEALTAEHAEAPHLRVLQKRL
EEECCCCCCCCEEEHEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
AKEVTVMVHSEEDYNAAVEASGILFGNATSEALKKLDEDTLLAVFEGVPQFEVSRDALAE
HHHEEEEEECCCCCCCEEEECEEEEECCHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHC
GVKAVDLFVDNAAVFASKGEMRKLVQGGGVSLNKEKLSAFDQVITTADLLDEKYLLVQRG
CCCEEEEEECCHHEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHEEECC
KKNYYLVIAK
CCCEEEEEEC
>Mature Secondary Structure
MNFVEELTWRGMVHTMMPGTEELLAKEQVTAYLGIDPTADSLHIGHLCGVMMLRHFQRCG
CCHHHHHHHCCHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
HKPLALVGGATGMIGDPSGKSQERNLLTEETLRHNVACIKKQLAKFLDFESDAPNKAELV
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
NNYDWMKDFTFLDFAREVGKHITVNYMMAKDSVQKRLNGEARDGLSFTEFTYQLLQGYDF
CCCHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE
LHLYETKGCKLQMGGSDQWGNITTGAELIRRTNGGEVFALTCPLITKADGGKFGKTESGN
EEEEECCCCEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCCCCCC
IWLDPRYTSPYKFYQFWLNVSDEDAARYIKIFTSLSQEEVEALTAEHAEAPHLRVLQKRL
EEECCCCCCCCEEEHEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
AKEVTVMVHSEEDYNAAVEASGILFGNATSEALKKLDEDTLLAVFEGVPQFEVSRDALAE
HHHEEEEEECCCCCCCEEEECEEEEECCHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHC
GVKAVDLFVDNAAVFASKGEMRKLVQGGGVSLNKEKLSAFDQVITTADLLDEKYLLVQRG
CCCEEEEEECCHHEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHEEECC
KKNYYLVIAK
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA