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The map label for this gene is queA

Identifier: 150003742

GI number: 150003742

Start: 1587181

End: 1588236

Strand: Direct

Name: queA

Synonym: BVU_1173

Alternate gene names: 150003742

Gene position: 1587181-1588236 (Clockwise)

Preceding gene: 150003741

Following gene: 150003743

Centisome position: 30.74

GC content: 46.4

Gene sequence:

>1056_bases
ATGAAACTGTCGCAATTTAAATTTAAACTGCCGGAGGATAAAATAGCTTTGCATCCGGCTAAGTATAGAGATGAGTCGCG
CCTGATGGTTGTTCACAAATCAACAGGTAAGATTGAACACAAGGTATTCAAGGATATCCTGGACTATTTTGATGATAAAG
ATGTGTTTATCTTTAATGATACGAAAGTTTTCCCTGCGCGTTTGTATGGAAATAAGGAAAAGACCGGTGCCCGTATCGAG
GTATTTTTGTTGCGCGAGCTGAATGAGGAACTTCGCTTGTGGGATGTGCTGGTAGATCCTGCCCGTAAGATCCGTATCGG
TAACAAGCTTTATTTTGGTGACGATGATTCCATGGTGGCCGAAGTGATTGACAACACCACTTCCCGCGGACGTACCCTGC
GTTTCCTGTATGACGGCTCTCACGACGAGTTCAAGAAAGCATTGTATGCGTTGGGAGAGGCTCCGCTGCCCAGTTTCATC
CGTCGTCCGGTAGAAGAGGAAGATGCCGAACGTTTCCAGACTATCTTTGCCAAGAACGAAGGAGCTGTGACAGCTCCCAC
TGCCGGACTTCATTTCAGCCGCGAGTTGATGAAGCGTATGGAAATCAAAGGTATAGACTTTGCTTTTGTGACGATGCATG
CCGGACTGGGTAATTTCCGCGAGATTGATGTGGAGGACCTTACCAAGCATAAGATGGATTCGGAGCAGATGTATGTCAAT
GCGGATGCGTGCCGTATTGTGAATAATGCCAAGGATGAAGGAAAGAATATTTGTGCGGTAGGAACGACAGTGATGCGCAC
GATAGAGACTGCGGTAGGTACGGATGGTCATCTGAAAGAATTTGACGGATGGACCAATAAGTTCATTTTCCCTCCTTATG
ATTTCTCGGTAGCCAACAGCATGGTTACTAACTTCCACCTGCCTCTCTCCACTTTGCTGATGCTGGTAGCTGCCTACGGT
GGTTATGATCTGGTGATGGAGGCTTATCACACAGCGTTGAAGGAAGATTACCGTTTCGGTACTTATGGCGACGCTATGTT
GATTTTGGATAAGTAA

Upstream 100 bases:

>100_bases
GGGATGTGAGACTGGAAGACTGTTTGTCGGTGGAAAGTTTTCCGGAATGGCTTGACCAACAAGAGATTGAAGAAGTTATA
AACGAATAAGGAGAGATAAG

Downstream 100 bases:

>100_bases
TACCGGTTGGAGCAGCGTATGAGGGTTTATTTGGGACTTGGAACCAATCTGGGTGATAAAGAATCAAATCTGCATGTTGC
TGTCCGGAAGATAAATGAGC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKLSQFKFKLPEDKIALHPAKYRDESRLMVVHKSTGKIEHKVFKDILDYFDDKDVFIFNDTKVFPARLYGNKEKTGARIE
VFLLRELNEELRLWDVLVDPARKIRIGNKLYFGDDDSMVAEVIDNTTSRGRTLRFLYDGSHDEFKKALYALGEAPLPSFI
RRPVEEEDAERFQTIFAKNEGAVTAPTAGLHFSRELMKRMEIKGIDFAFVTMHAGLGNFREIDVEDLTKHKMDSEQMYVN
ADACRIVNNAKDEGKNICAVGTTVMRTIETAVGTDGHLKEFDGWTNKFIFPPYDFSVANSMVTNFHLPLSTLLMLVAAYG
GYDLVMEAYHTALKEDYRFGTYGDAMLILDK

Sequences:

>Translated_351_residues
MKLSQFKFKLPEDKIALHPAKYRDESRLMVVHKSTGKIEHKVFKDILDYFDDKDVFIFNDTKVFPARLYGNKEKTGARIE
VFLLRELNEELRLWDVLVDPARKIRIGNKLYFGDDDSMVAEVIDNTTSRGRTLRFLYDGSHDEFKKALYALGEAPLPSFI
RRPVEEEDAERFQTIFAKNEGAVTAPTAGLHFSRELMKRMEIKGIDFAFVTMHAGLGNFREIDVEDLTKHKMDSEQMYVN
ADACRIVNNAKDEGKNICAVGTTVMRTIETAVGTDGHLKEFDGWTNKFIFPPYDFSVANSMVTNFHLPLSTLLMLVAAYG
GYDLVMEAYHTALKEDYRFGTYGDAMLILDK
>Mature_351_residues
MKLSQFKFKLPEDKIALHPAKYRDESRLMVVHKSTGKIEHKVFKDILDYFDDKDVFIFNDTKVFPARLYGNKEKTGARIE
VFLLRELNEELRLWDVLVDPARKIRIGNKLYFGDDDSMVAEVIDNTTSRGRTLRFLYDGSHDEFKKALYALGEAPLPSFI
RRPVEEEDAERFQTIFAKNEGAVTAPTAGLHFSRELMKRMEIKGIDFAFVTMHAGLGNFREIDVEDLTKHKMDSEQMYVN
ADACRIVNNAKDEGKNICAVGTTVMRTIETAVGTDGHLKEFDGWTNKFIFPPYDFSVANSMVTNFHLPLSTLLMLVAAYG
GYDLVMEAYHTALKEDYRFGTYGDAMLILDK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=350, Percent_Identity=39.7142857142857, Blast_Score=254, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BACV8 (A6KZK0)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298486.1
- ProteinModelPortal:   A6KZK0
- SMR:   A6KZK0
- STRING:   A6KZK0
- GeneID:   5302139
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1173
- NMPDR:   fig|435590.6.peg.1111
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   RKVRVGN
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40180; Mature: 40180

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSQFKFKLPEDKIALHPAKYRDESRLMVVHKSTGKIEHKVFKDILDYFDDKDVFIFND
CCCCCCEEECCCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
TKVFPARLYGNKEKTGARIEVFLLRELNEELRLWDVLVDPARKIRIGNKLYFGDDDSMVA
CEEEEHHHCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHCCHHHEEECCEEEECCCHHHHH
EVIDNTTSRGRTLRFLYDGSHDEFKKALYALGEAPLPSFIRRPVEEEDAERFQTIFAKNE
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHCCC
GAVTAPTAGLHFSRELMKRMEIKGIDFAFVTMHAGLGNFREIDVEDLTKHKMDSEQMYVN
CCEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECHHHHHHHCCCCCCEEEE
ADACRIVNNAKDEGKNICAVGTTVMRTIETAVGTDGHLKEFDGWTNKFIFPPYDFSVANS
CHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHCCCCCCCEECCCCCHHHHHH
MVTNFHLPLSTLLMLVAAYGGYDLVMEAYHTALKEDYRFGTYGDAMLILDK
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKLSQFKFKLPEDKIALHPAKYRDESRLMVVHKSTGKIEHKVFKDILDYFDDKDVFIFND
CCCCCCEEECCCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
TKVFPARLYGNKEKTGARIEVFLLRELNEELRLWDVLVDPARKIRIGNKLYFGDDDSMVA
CEEEEHHHCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHCCHHHEEECCEEEECCCHHHHH
EVIDNTTSRGRTLRFLYDGSHDEFKKALYALGEAPLPSFIRRPVEEEDAERFQTIFAKNE
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHCCC
GAVTAPTAGLHFSRELMKRMEIKGIDFAFVTMHAGLGNFREIDVEDLTKHKMDSEQMYVN
CCEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECHHHHHHHCCCCCCEEEE
ADACRIVNNAKDEGKNICAVGTTVMRTIETAVGTDGHLKEFDGWTNKFIFPPYDFSVANS
CHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHCCCCCCCEECCCCCHHHHHH
MVTNFHLPLSTLLMLVAAYGGYDLVMEAYHTALKEDYRFGTYGDAMLILDK
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA