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The map label for this gene is dacB [H]

Identifier: 150003677

GI number: 150003677

Start: 1475275

End: 1476675

Strand: Reverse

Name: dacB [H]

Synonym: BVU_1108

Alternate gene names: 150003677

Gene position: 1476675-1475275 (Counterclockwise)

Preceding gene: 150003678

Following gene: 150003676

Centisome position: 28.6

GC content: 46.11

Gene sequence:

>1401_bases
ATGAAACGAGTTTTATTTTTCTTTTTACTGATTTTATCTATTCACTTCATAAACGCCCAGCCGCTATCTCAGCGGCTGGA
CGCTTTACTGCATGAGGAAGTTCTGAAAACATCCGAAGTGGGTATTGCCGTATTCGATCTGACCACCGGGAAATCCGTTT
ACCGTTATCAGGACGAGAAACTATACCGTCCTGCTTCTGTTGAGAAGATAATCACTTCGGTTACCGCCTTGGCGCAACTG
GGAGCGGATTATACCATGGATACCGGTCTTCGTTATCGTGGAAAAATAGAAAATGACACGTTGAAAGGCAGTCTGTATCT
GATAGGCGGTTTTGATCCGGAATTTATGGACGAGGATCTGGACCGTCTGGTAGATGCGTTGGCAAGTCAAGGCATCCGTT
ATGTGACAGACACCCTTGCGGCGGATGTTTCCATGACGGACTCGGTTTATTGGGGTTCCGGCTGGTGTTGGGACGATACT
CCTTACTCCTTCCAGCCCTATCTTTCCCCTCTCATGCTGAATAGGGGCTGCGTGGATGTATCGGTCTCTCCTGCTCAGAA
GGACTCGTTGCCTAAAGTCGTTTGTACGCCGGTTTCCGATTATTATCAGGTTCATAATCATGGGGTGAGCCGTAATCCGC
AAGCCGGGAAGCTGAAAATCACCCGCAACTGGCTCAGCAATGGCAATATCATTACCGTTTCCGGAAATGTTTCTTATCCC
TATACTGAGAAATTGAATGTATATACTTCCAAAGATTTCTTTTTCCATACCTTTGTCAGCCGTTTGCGCAGCAAAGGAAT
TGAAGCGCGAACTTGCACGTATGCCGATTGTCCTGTAACGGCCGACAGTATAGTTACGCTTTATACCGTCCGAAGACCAC
TCAAGGAAGTGTTGGAAAGAGCTTTGAAGAAAAGTGATAACTTGTGCGCCGAATCGATGTTTTATCATTTGGCTGCCAAG
CGTTCCCTGCATAAACGGGTGACGGGAGAAGATGGAACCGATGCCATTCACGCCTTTATGAAAACAGCTTTAGGGTTTAA
CCCGGAGAACTATAAGATAGCGGACGGCAGCGGTGTTTCCGTTTATAATTATATCTCTCCACGTTTACTATTGGAGTATT
TGAAATATGCCTACTATCATCGGGAAATTTTTCTTCCTTTCTATGAGTCCCTGCCCATAGCCGGAGTGGACGGTACTTTA
CAAAACCGGATGAAGCAGACCAAGGCCCGTGGAAATGTGCATGCAAAAACAGGTTCTGTAACCGGAGTCAGCTCGCTGGC
AGGCTATGTAAAAGCTGCTGACGGACATCAACTGGCCTTTGTTATCATCAATCAGAATGTACTGAAATTAAGCCGGGCGC
GTGCATTTCAAGATAAGTTTTGTGATATTTTATCTCGTTGA

Upstream 100 bases:

>100_bases
TATTGGCAGGTATGGCTATTGAGGATGGTAAGACCATAGAGGATTGGAAACGATATGTATTTCCTCATCCTACTGTGGGT
GAGATATTCAGGGAACTATA

Downstream 100 bases:

>100_bases
ATTTGAGTTTTTGAATGTTCTGAAACGTATTAAGTAGCAGATTTTGCATATAATAGGACAAAATTTATACCTATTCTGAA
CGATTTTTGCAGCATCGGAA

Product: putative exported D-alanyl-D-alanine carboxypeptidase penicillin-binding protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKRVLFFFLLILSIHFINAQPLSQRLDALLHEEVLKTSEVGIAVFDLTTGKSVYRYQDEKLYRPASVEKIITSVTALAQL
GADYTMDTGLRYRGKIENDTLKGSLYLIGGFDPEFMDEDLDRLVDALASQGIRYVTDTLAADVSMTDSVYWGSGWCWDDT
PYSFQPYLSPLMLNRGCVDVSVSPAQKDSLPKVVCTPVSDYYQVHNHGVSRNPQAGKLKITRNWLSNGNIITVSGNVSYP
YTEKLNVYTSKDFFFHTFVSRLRSKGIEARTCTYADCPVTADSIVTLYTVRRPLKEVLERALKKSDNLCAESMFYHLAAK
RSLHKRVTGEDGTDAIHAFMKTALGFNPENYKIADGSGVSVYNYISPRLLLEYLKYAYYHREIFLPFYESLPIAGVDGTL
QNRMKQTKARGNVHAKTGSVTGVSSLAGYVKAADGHQLAFVIINQNVLKLSRARAFQDKFCDILSR

Sequences:

>Translated_466_residues
MKRVLFFFLLILSIHFINAQPLSQRLDALLHEEVLKTSEVGIAVFDLTTGKSVYRYQDEKLYRPASVEKIITSVTALAQL
GADYTMDTGLRYRGKIENDTLKGSLYLIGGFDPEFMDEDLDRLVDALASQGIRYVTDTLAADVSMTDSVYWGSGWCWDDT
PYSFQPYLSPLMLNRGCVDVSVSPAQKDSLPKVVCTPVSDYYQVHNHGVSRNPQAGKLKITRNWLSNGNIITVSGNVSYP
YTEKLNVYTSKDFFFHTFVSRLRSKGIEARTCTYADCPVTADSIVTLYTVRRPLKEVLERALKKSDNLCAESMFYHLAAK
RSLHKRVTGEDGTDAIHAFMKTALGFNPENYKIADGSGVSVYNYISPRLLLEYLKYAYYHREIFLPFYESLPIAGVDGTL
QNRMKQTKARGNVHAKTGSVTGVSSLAGYVKAADGHQLAFVIINQNVLKLSRARAFQDKFCDILSR
>Mature_466_residues
MKRVLFFFLLILSIHFINAQPLSQRLDALLHEEVLKTSEVGIAVFDLTTGKSVYRYQDEKLYRPASVEKIITSVTALAQL
GADYTMDTGLRYRGKIENDTLKGSLYLIGGFDPEFMDEDLDRLVDALASQGIRYVTDTLAADVSMTDSVYWGSGWCWDDT
PYSFQPYLSPLMLNRGCVDVSVSPAQKDSLPKVVCTPVSDYYQVHNHGVSRNPQAGKLKITRNWLSNGNIITVSGNVSYP
YTEKLNVYTSKDFFFHTFVSRLRSKGIEARTCTYADCPVTADSIVTLYTVRRPLKEVLERALKKSDNLCAESMFYHLAAK
RSLHKRVTGEDGTDAIHAFMKTALGFNPENYKIADGSGVSVYNYISPRLLLEYLKYAYYHREIFLPFYESLPIAGVDGTL
QNRMKQTKARGNVHAKTGSVTGVSSLAGYVKAADGHQLAFVIINQNVLKLSRARAFQDKFCDILSR

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=395, Percent_Identity=30.6329113924051, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52346; Mature: 52346

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVLFFFLLILSIHFINAQPLSQRLDALLHEEVLKTSEVGIAVFDLTTGKSVYRYQDEK
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCHH
LYRPASVEKIITSVTALAQLGADYTMDTGLRYRGKIENDTLKGSLYLIGGFDPEFMDEDL
HCCCHHHHHHHHHHHHHHHHCCCCEECCCCEECCCCCCCEECEEEEEEECCCHHHHHHHH
DRLVDALASQGIRYVTDTLAADVSMTDSVYWGSGWCWDDTPYSFQPYLSPLMLNRGCVDV
HHHHHHHHHCCCHHHHHHHHHCCCCCCCEEECCEEEECCCCCCCCHHHHHHHHCCCCEEE
SVSPAQKDSLPKVVCTPVSDYYQVHNHGVSRNPQAGKLKITRNWLSNGNIITVSGNVSYP
EECCCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
YTEKLNVYTSKDFFFHTFVSRLRSKGIEARTCTYADCPVTADSIVTLYTVRRPLKEVLER
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ALKKSDNLCAESMFYHLAAKRSLHKRVTGEDGTDAIHAFMKTALGFNPENYKIADGSGVS
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCE
VYNYISPRLLLEYLKYAYYHREIFLPFYESLPIAGVDGTLQNRMKQTKARGNVHAKTGSV
EEHHCCHHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
TGVSSLAGYVKAADGHQLAFVIINQNVLKLSRARAFQDKFCDILSR
CCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRVLFFFLLILSIHFINAQPLSQRLDALLHEEVLKTSEVGIAVFDLTTGKSVYRYQDEK
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCHH
LYRPASVEKIITSVTALAQLGADYTMDTGLRYRGKIENDTLKGSLYLIGGFDPEFMDEDL
HCCCHHHHHHHHHHHHHHHHCCCCEECCCCEECCCCCCCEECEEEEEEECCCHHHHHHHH
DRLVDALASQGIRYVTDTLAADVSMTDSVYWGSGWCWDDTPYSFQPYLSPLMLNRGCVDV
HHHHHHHHHCCCHHHHHHHHHCCCCCCCEEECCEEEECCCCCCCCHHHHHHHHCCCCEEE
SVSPAQKDSLPKVVCTPVSDYYQVHNHGVSRNPQAGKLKITRNWLSNGNIITVSGNVSYP
EECCCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
YTEKLNVYTSKDFFFHTFVSRLRSKGIEARTCTYADCPVTADSIVTLYTVRRPLKEVLER
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ALKKSDNLCAESMFYHLAAKRSLHKRVTGEDGTDAIHAFMKTALGFNPENYKIADGSGVS
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCE
VYNYISPRLLLEYLKYAYYHREIFLPFYESLPIAGVDGTLQNRMKQTKARGNVHAKTGSV
EEHHCCHHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
TGVSSLAGYVKAADGHQLAFVIINQNVLKLSRARAFQDKFCDILSR
CCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]