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The map label for this gene is pdhD [H]

Identifier: 150003678

GI number: 150003678

Start: 1476675

End: 1478015

Strand: Reverse

Name: pdhD [H]

Synonym: BVU_1109

Alternate gene names: 150003678

Gene position: 1478015-1476675 (Counterclockwise)

Preceding gene: 150003679

Following gene: 150003677

Centisome position: 28.63

GC content: 47.05

Gene sequence:

>1341_bases
ATGAAATATCAAATTGCAATCATCGGTGGAGGTCCCGCAGGGTATACTGCTGCCGAAGCTGCCGGAAAGGCAGGACTGAG
TGTTGTATTGTTCGAAAAAAGAAGTCTGGGCGGTGTCTGCCTGAATGAGGGATGTATTCCTACCAAGACCTTGCTTTATT
CGGCTAAAGTATATGATTATGCGAAACATGCTTCAAAGTATGCCGTGAATGTGCCTGAAGCTTCTTTTGATTTAGGAAAG
ATTGTGGCGCGTAAATCAAAGGTGGTTCGTAAGCTGGTATTGGGAATCAAGGCAAAACTGACTGCGCATCAGGTACATAT
AGTGACGGGAGAAGCCACGATTGTAGATAAGAATACCATCCAATGCGGTGGGGAAACCTACGAATGTGAGAATTTGCTGC
TGTGTACCGGCAGTGAAACATTTATTCCTCCTATTCCGGGAGTGGAAAACGTGGATTACTGGACGCACCGTGATGCACTG
GATAATAAAGAAGTACCTGCTTCATTGACTATTGTCGGCGGTGGAGTGATCGGTATGGAATTTGCTTCTTTCTTCACCAG
TTTGGGAGTGCAGGTGACTGTGGTCGAAATGTTGGATGAAATCCTGGGTGGCATGGATAAAGAGCTGTCGGCCATGCTCC
GGGCTGAATATGCCAAAAAAGGAATCAAGTTCCTGCTCAGTACGAAAGTAGTAGGGGTATCCAAAGGGGAGACCGGTATT
ACTGTGTCATACGAAAATGCAGATGGAACAGGAACTGTTACAGCTGATAAGTTATTGATGAGTGTAGGCCGTCGTCCGGT
GACAAAAGGTTTCGGACTGGAAAATCTGAATTTGGAATGGACGGAGCGCAGATGCATCAAAGTAGATGAACATTTGCAAT
CTTCCGTTCCCGGTGTCTATGTATGCGGTGATTTGAACGGAGTCTCTTTGTTGGCACATACCGCCGTGCGTGAAGCGGAA
GTGGCTGTCCATCATATTACAGGTAAGGAAGATGTGATGAGCTATCGTGCCATACCGGGTGTGGTCTATACAAATCCTGA
GATTGCCGGGGTAGGTATGAGCGAGGAAGCTTTGCAGGCCGCAGGTATTCCTTATCGAGCCGTTAAGTTGCCGATGGCTT
ACTCCGGGCGTTTTGTAGCCGAGAACGAGGGAGTGAACGGTGTCTGCAAAGTTTTGACAGCCGAAGACGGTACTGTATTG
GGAGCGCATATGTTGGGTAATCCTGCTTCCGAGCTGATTGTATTGGCAGGTATGGCTATTGAGGATGGTAAGACCATAGA
GGATTGGAAACGATATGTATTTCCTCATCCTACTGTGGGTGAGATATTCAGGGAACTATAA

Upstream 100 bases:

>100_bases
TAAGCCTTTGGAATTTGTAGAAGCATTGAGAGAAATAGATCCAAATCTGTCTGTTGAAGGTTTTGAACAGATAAAAACTA
ATTAACCCAAAATAAATACC

Downstream 100 bases:

>100_bases
TGAAACGAGTTTTATTTTTCTTTTTACTGATTTTATCTATTCACTTCATAAACGCCCAGCCGCTATCTCAGCGGCTGGAC
GCTTTACTGCATGAGGAAGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKYQIAIIGGGPAGYTAAEAAGKAGLSVVLFEKRSLGGVCLNEGCIPTKTLLYSAKVYDYAKHASKYAVNVPEASFDLGK
IVARKSKVVRKLVLGIKAKLTAHQVHIVTGEATIVDKNTIQCGGETYECENLLLCTGSETFIPPIPGVENVDYWTHRDAL
DNKEVPASLTIVGGGVIGMEFASFFTSLGVQVTVVEMLDEILGGMDKELSAMLRAEYAKKGIKFLLSTKVVGVSKGETGI
TVSYENADGTGTVTADKLLMSVGRRPVTKGFGLENLNLEWTERRCIKVDEHLQSSVPGVYVCGDLNGVSLLAHTAVREAE
VAVHHITGKEDVMSYRAIPGVVYTNPEIAGVGMSEEALQAAGIPYRAVKLPMAYSGRFVAENEGVNGVCKVLTAEDGTVL
GAHMLGNPASELIVLAGMAIEDGKTIEDWKRYVFPHPTVGEIFREL

Sequences:

>Translated_446_residues
MKYQIAIIGGGPAGYTAAEAAGKAGLSVVLFEKRSLGGVCLNEGCIPTKTLLYSAKVYDYAKHASKYAVNVPEASFDLGK
IVARKSKVVRKLVLGIKAKLTAHQVHIVTGEATIVDKNTIQCGGETYECENLLLCTGSETFIPPIPGVENVDYWTHRDAL
DNKEVPASLTIVGGGVIGMEFASFFTSLGVQVTVVEMLDEILGGMDKELSAMLRAEYAKKGIKFLLSTKVVGVSKGETGI
TVSYENADGTGTVTADKLLMSVGRRPVTKGFGLENLNLEWTERRCIKVDEHLQSSVPGVYVCGDLNGVSLLAHTAVREAE
VAVHHITGKEDVMSYRAIPGVVYTNPEIAGVGMSEEALQAAGIPYRAVKLPMAYSGRFVAENEGVNGVCKVLTAEDGTVL
GAHMLGNPASELIVLAGMAIEDGKTIEDWKRYVFPHPTVGEIFREL
>Mature_446_residues
MKYQIAIIGGGPAGYTAAEAAGKAGLSVVLFEKRSLGGVCLNEGCIPTKTLLYSAKVYDYAKHASKYAVNVPEASFDLGK
IVARKSKVVRKLVLGIKAKLTAHQVHIVTGEATIVDKNTIQCGGETYECENLLLCTGSETFIPPIPGVENVDYWTHRDAL
DNKEVPASLTIVGGGVIGMEFASFFTSLGVQVTVVEMLDEILGGMDKELSAMLRAEYAKKGIKFLLSTKVVGVSKGETGI
TVSYENADGTGTVTADKLLMSVGRRPVTKGFGLENLNLEWTERRCIKVDEHLQSSVPGVYVCGDLNGVSLLAHTAVREAE
VAVHHITGKEDVMSYRAIPGVVYTNPEIAGVGMSEEALQAAGIPYRAVKLPMAYSGRFVAENEGVNGVCKVLTAEDGTVL
GAHMLGNPASELIVLAGMAIEDGKTIEDWKRYVFPHPTVGEIFREL

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=38.3442265795207, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI50301238, Length=456, Percent_Identity=28.2894736842105, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI22035672, Length=431, Percent_Identity=28.3062645011601, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045266, Length=438, Percent_Identity=25.7990867579909, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI148277065, Length=434, Percent_Identity=23.2718894009217, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI33519430, Length=434, Percent_Identity=23.2718894009217, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI33519428, Length=434, Percent_Identity=23.2718894009217, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI33519426, Length=434, Percent_Identity=23.2718894009217, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI148277071, Length=434, Percent_Identity=23.2718894009217, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI291045268, Length=432, Percent_Identity=24.3055555555556, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=34.8214285714286, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=29.5652173913043, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=27.5488069414317, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=25.5760368663594, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789065, Length=205, Percent_Identity=29.2682926829268, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32565766, Length=453, Percent_Identity=39.514348785872, Blast_Score=285, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI71983429, Length=432, Percent_Identity=27.3148148148148, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=432, Percent_Identity=27.3148148148148, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71982272, Length=441, Percent_Identity=26.984126984127, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=24.8945147679325, Blast_Score=111, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=465, Percent_Identity=34.6236559139785, Blast_Score=236, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6325240, Length=466, Percent_Identity=31.9742489270386, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=27.2138228941685, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=38.646288209607, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=29.0526315789474, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640553, Length=471, Percent_Identity=28.6624203821656, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640549, Length=471, Percent_Identity=28.6624203821656, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=28.2474226804124, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47673; Mature: 47673

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQIAIIGGGPAGYTAAEAAGKAGLSVVLFEKRSLGGVCLNEGCIPTKTLLYSAKVYDY
CEEEEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCHHHHEEHHHHHHH
AKHASKYAVNVPEASFDLGKIVARKSKVVRKLVLGIKAKLTAHQVHIVTGEATIVDKNTI
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEECCEEEEECCEE
QCGGETYECENLLLCTGSETFIPPIPGVENVDYWTHRDALDNKEVPASLTIVGGGVIGME
EECCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHH
FASFFTSLGVQVTVVEMLDEILGGMDKELSAMLRAEYAKKGIKFLLSTKVVGVSKGETGI
HHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEHEEEEEECCCCCCE
TVSYENADGTGTVTADKLLMSVGRRPVTKGFGLENLNLEWTERRCIKVDEHLQSSVPGVY
EEEEECCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHEEHHHHHHHCCCCEE
VCGDLNGVSLLAHTAVREAEVAVHHITGKEDVMSYRAIPGVVYTNPEIAGVGMSEEALQA
EEECCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCEEECCCCEEECCCCHHHHHH
AGIPYRAVKLPMAYSGRFVAENEGVNGVCKVLTAEDGTVLGAHMLGNPASELIVLAGMAI
CCCCEEEEECCEEECCEEEECCCCCCCEEEEEECCCCCEEEHHHCCCCHHHEEEEEEEEE
EDGKTIEDWKRYVFPHPTVGEIFREL
CCCCCHHHHHHHCCCCCCHHHHHHHC
>Mature Secondary Structure
MKYQIAIIGGGPAGYTAAEAAGKAGLSVVLFEKRSLGGVCLNEGCIPTKTLLYSAKVYDY
CEEEEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCHHHHEEHHHHHHH
AKHASKYAVNVPEASFDLGKIVARKSKVVRKLVLGIKAKLTAHQVHIVTGEATIVDKNTI
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEECCEEEEECCEE
QCGGETYECENLLLCTGSETFIPPIPGVENVDYWTHRDALDNKEVPASLTIVGGGVIGME
EECCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHH
FASFFTSLGVQVTVVEMLDEILGGMDKELSAMLRAEYAKKGIKFLLSTKVVGVSKGETGI
HHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEHEEEEEECCCCCCE
TVSYENADGTGTVTADKLLMSVGRRPVTKGFGLENLNLEWTERRCIKVDEHLQSSVPGVY
EEEEECCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHEEHHHHHHHCCCCEE
VCGDLNGVSLLAHTAVREAEVAVHHITGKEDVMSYRAIPGVVYTNPEIAGVGMSEEALQA
EEECCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCEEECCCCEEECCCCHHHHHH
AGIPYRAVKLPMAYSGRFVAENEGVNGVCKVLTAEDGTVLGAHMLGNPASELIVLAGMAI
CCCCEEEEECCEEECCEEEECCCCCCCEEEEEECCCCCEEEHHHCCCCHHHEEEEEEEEE
EDGKTIEDWKRYVFPHPTVGEIFREL
CCCCCHHHHHHHCCCCCCHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]