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The map label for this gene is dus1 [H]

Identifier: 150003654

GI number: 150003654

Start: 1444858

End: 1445847

Strand: Direct

Name: dus1 [H]

Synonym: BVU_1085

Alternate gene names: 150003654

Gene position: 1444858-1445847 (Clockwise)

Preceding gene: 150003652

Following gene: 150003660

Centisome position: 27.98

GC content: 46.06

Gene sequence:

>990_bases
ATGAAGATTAGAAATATAGACCTGGGCAGATACCCTATTTTCCTGGCTCCGATGGAAGATGTAACAGACCCCGCTTTTCG
CCTGATGTGCAAGAAGTTCGGTGCTGATATGGTATATACCGAATTTGTATCGGCAGATGCATTGATACGCTCGGTAGGTA
AAACGATGCAGAAGCTGAATATCAACGATGAGGAACGCCCCGTAGCCATCCAGATTTACGGCAGAGACACGGAAACAATG
GTAGCAGCGGCGAAAATAGTGGAAGAGGCGCATCCTGACATCCTGGATATTAATTTTGGCTGTCCTGTAAAACGCGTTGC
CGGTAAAGGAGCCGGTGCAGGTATGCTGCAAAATATCCCGCTGATGTTGGAAATTACCCGTGCTGTTGTGGACACTGTAA
AAATTCCCGTTACTGTAAAAACACGTCTAGGCTGGGACAATGAGCATAAAATTATAGTAGATTTGGCAGAACAATTACAA
GATTGCGGCATTGAAGCACTGACCATTCATGGACGCACCCGTGCACAAATGTACACTGGTGAAGCGGACTGGAGTTTAAT
AGGTGAAGTAAAAAAGAATCCCCGTATGCATATACCTATCATTGGAAACGGGGATATAACTACGCCACAACGTGCCAAAG
AGTGTTTTGACCAGTATGGAGTGGACGCTATTATGATTGGCCGAGCCAGCTTTGGACGCCCGTGGATATTCAAGGAAGTA
AAACATTATATAGAAACCGGTAAGGAACTCCCGGCCCTTTCTTTTGAGTGGCGGCTGAATGTACTCCGTCAGGAAGTGTT
GGACAGTGTGAATCTGCTTGACGAACGCCGCGGTATTCTTCATGTACGCCGCCATCTGGCTGCTTCTCCCTTATTTAAAG
GCATCCCCAACTTTAAAGAGACACGTATCGCCATGCTCCGTGCCGAAACCGTGAAAGAACTTTTCAGTATCTTGGATTAT
ATCAGGGGGAATTACGGAATGAATTCTTAA

Upstream 100 bases:

>100_bases
GCAGCCTGATTTCACCACAGATTACACAGATTCACACAGATTATAAATTAATACTCAAATGAATCTGACTTTTCTGTAAC
CCAAAGATAAACATACAACA

Downstream 100 bases:

>100_bases
TGCTACAATGTGCTAATATGCTAATGTGCCAATTGCCATGCGGAATACAGCACAGCCAATTGGCACATTAATATATTGTA
GCATTAACACATTATCTATT

Product: dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKIRNIDLGRYPIFLAPMEDVTDPAFRLMCKKFGADMVYTEFVSADALIRSVGKTMQKLNINDEERPVAIQIYGRDTETM
VAAAKIVEEAHPDILDINFGCPVKRVAGKGAGAGMLQNIPLMLEITRAVVDTVKIPVTVKTRLGWDNEHKIIVDLAEQLQ
DCGIEALTIHGRTRAQMYTGEADWSLIGEVKKNPRMHIPIIGNGDITTPQRAKECFDQYGVDAIMIGRASFGRPWIFKEV
KHYIETGKELPALSFEWRLNVLRQEVLDSVNLLDERRGILHVRRHLAASPLFKGIPNFKETRIAMLRAETVKELFSILDY
IRGNYGMNS

Sequences:

>Translated_329_residues
MKIRNIDLGRYPIFLAPMEDVTDPAFRLMCKKFGADMVYTEFVSADALIRSVGKTMQKLNINDEERPVAIQIYGRDTETM
VAAAKIVEEAHPDILDINFGCPVKRVAGKGAGAGMLQNIPLMLEITRAVVDTVKIPVTVKTRLGWDNEHKIIVDLAEQLQ
DCGIEALTIHGRTRAQMYTGEADWSLIGEVKKNPRMHIPIIGNGDITTPQRAKECFDQYGVDAIMIGRASFGRPWIFKEV
KHYIETGKELPALSFEWRLNVLRQEVLDSVNLLDERRGILHVRRHLAASPLFKGIPNFKETRIAMLRAETVKELFSILDY
IRGNYGMNS
>Mature_329_residues
MKIRNIDLGRYPIFLAPMEDVTDPAFRLMCKKFGADMVYTEFVSADALIRSVGKTMQKLNINDEERPVAIQIYGRDTETM
VAAAKIVEEAHPDILDINFGCPVKRVAGKGAGAGMLQNIPLMLEITRAVVDTVKIPVTVKTRLGWDNEHKIIVDLAEQLQ
DCGIEALTIHGRTRAQMYTGEADWSLIGEVKKNPRMHIPIIGNGDITTPQRAKECFDQYGVDAIMIGRASFGRPWIFKEV
KHYIETGKELPALSFEWRLNVLRQEVLDSVNLLDERRGILHVRRHLAASPLFKGIPNFKETRIAMLRAETVKELFSILDY
IRGNYGMNS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=258, Percent_Identity=31.7829457364341, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI239788462, Length=234, Percent_Identity=32.4786324786325, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=30.1310043668122, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI31742496, Length=322, Percent_Identity=25.776397515528, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8923374, Length=244, Percent_Identity=27.4590163934426, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789660, Length=287, Percent_Identity=39.7212543554007, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=34.1666666666667, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI145693211, Length=307, Percent_Identity=28.6644951140065, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17507177, Length=237, Percent_Identity=32.4894514767932, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17543114, Length=233, Percent_Identity=29.6137339055794, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=221, Percent_Identity=29.8642533936652, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=253, Percent_Identity=28.8537549407115, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=30.5084745762712, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323433, Length=274, Percent_Identity=27.7372262773723, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=240, Percent_Identity=26.25, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585320, Length=242, Percent_Identity=29.3388429752066, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24580595, Length=236, Percent_Identity=33.0508474576271, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19920448, Length=236, Percent_Identity=33.0508474576271, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=27.7533039647577, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI45549423, Length=263, Percent_Identity=30.4182509505703, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37153; Mature: 37153

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRNIDLGRYPIFLAPMEDVTDPAFRLMCKKFGADMVYTEFVSADALIRSVGKTMQKLN
CCCCCCCCCCCCEEEECHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
INDEERPVAIQIYGRDTETMVAAAKIVEEAHPDILDINFGCPVKRVAGKGAGAGMLQNIP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCHHHHCCC
LMLEITRAVVDTVKIPVTVKTRLGWDNEHKIIVDLAEQLQDCGIEALTIHGRTRAQMYTG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEEC
EADWSLIGEVKKNPRMHIPIIGNGDITTPQRAKECFDQYGVDAIMIGRASFGRPWIFKEV
CCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
KHYIETGKELPALSFEWRLNVLRQEVLDSVNLLDERRGILHVRRHLAASPLFKGIPNFKE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHH
TRIAMLRAETVKELFSILDYIRGNYGMNS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIRNIDLGRYPIFLAPMEDVTDPAFRLMCKKFGADMVYTEFVSADALIRSVGKTMQKLN
CCCCCCCCCCCCEEEECHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
INDEERPVAIQIYGRDTETMVAAAKIVEEAHPDILDINFGCPVKRVAGKGAGAGMLQNIP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCHHHHCCC
LMLEITRAVVDTVKIPVTVKTRLGWDNEHKIIVDLAEQLQDCGIEALTIHGRTRAQMYTG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEEC
EADWSLIGEVKKNPRMHIPIIGNGDITTPQRAKECFDQYGVDAIMIGRASFGRPWIFKEV
CCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
KHYIETGKELPALSFEWRLNVLRQEVLDSVNLLDERRGILHVRRHLAASPLFKGIPNFKE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHH
TRIAMLRAETVKELFSILDYIRGNYGMNS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]