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The map label for this gene is pyk [H]

Identifier: 150003449

GI number: 150003449

Start: 1178009

End: 1179472

Strand: Reverse

Name: pyk [H]

Synonym: BVU_0876

Alternate gene names: 150003449

Gene position: 1179472-1178009 (Counterclockwise)

Preceding gene: 150003450

Following gene: 150003448

Centisome position: 22.84

GC content: 45.56

Gene sequence:

>1464_bases
ATGATGTTGAAACAAACAAAAATCGTAGCGTCCATCTCAGATCAGCGTTGCGAGGTAGAGTTTATAAGAGATCTTTTCAA
GGCCGGTATGAATGTGGTCCGTATGAATACGGCTCACGCTTCACGTGAAGGTTTTGAAAAATTGATAACCAATGTACGTG
AGGTTTCCAACCGTATTGCCATATTGATGGACACAAAAGGGCCCGAAGTGCGTACTACTGCTATTGCGGGCGGCGAGCCT
ATTCCTTATCAGATTGGTGACAAAGTGAAGATTGTAGGCAATCCTGCACAAGAAACGACCCGTGAATGTATTGCTGTATC
TTATCCCGGTTTTGTGCATGATCTCCAGGTGGATGGCGATATTCTGATTGATGATGGTGATCTGGAGCTGCGTGTGATTG
AGAAGACAGACGAGTATTTGTTGTGTGAGGTGCAGAATGAAGCTACTTTGGGTAACCGTAAGAGTGTAAATGTACCGGGT
GTGCGCATCAATCTCCCTTCTTTGACAGAAAAGGACCGTAACAATATACTGTATGCTATTGAAAAGGACATTGATTTCAT
AGCACATTCGTTTGTTCGTAATAAGCAGGATGTGCTGGATATCCGTCAGATACTGGATGCATACGGCAGTGACATTAAGA
TTATCGCCAAGATCGAAAATCAGGAAGGAGTGGACAATATTGATGAAATATTGGAAGTGGCCGACGGAGTCATGGTGGCT
CGTGGTGACTTGGGTATTGAGGTTCCTCAGGAACGAATTCCCGGCATTCAGCGTCTGTTGATCAAAAAATGTATTCTGGC
AAAGAAACCGGTGATTGTTGCTACCCAGATGTTGCATACCATGATTAATAATCCGCGTCCTACCCGTGCTGAAGTGACCG
ATATTGCCAATGCTATTTACTACCGCACGGATGCATTGATGCTGAGTGGGGAAACGGCATACGGCAAGTATCCGGTGGAA
GCCGTGAAGACGATGGCCAAGATTGCAGCGCAGGCCGAGAAAGATAAACTGGCCGAGAATGACATCCGTATTCCTTTGGA
TGAAAACTCCAATGATGTAACCGCTTTCCTGGCAAAACAGGCAGTAAAGGCTACCAGCAAACTGAATATCCGCGCCATTA
TTACCGACAGTTTCAGCGGGCGTACCGCACGTAATATTGCTGCTTTCCGTGGCAAATATCCTGTATTGGCCATCTGTTAT
AAAGAAAAAACGATGCGTCATCTGGCTTTGTCATATGGGGTGGAAGCTATCTATATGCCCGAAAAGGCTAACGGACAGGC
ATATTATTTCGCTGCTTTGCGTAAATTGCTGGATGATGGTGTCTTGAGTGAAAGCGACATGGTGGCTTACCTGAGCAGCG
GCAAGCAAGGTACTCAGACTTCTTTCCTTGAAATCAATGTAGTGGGAGATGTGTTGAAGTATGCTATGGACTATGTGCTG
CCCAACCGCAACCGTTACCTATAA

Upstream 100 bases:

>100_bases
GAGTGATTTGTGGGTTCGGGCTTCATTCATATCGTTTGGCGATTGAGGCTTTGTTAATGAGAAAATAAAACGTTTAGGTA
TAATATAAGGTGATAAGATT

Downstream 100 bases:

>100_bases
TAATAGAAGATAGGAATGAAAGAAACGGACGCTATTGACGAATATATCCTTCAGCACATTGATGAAGAAAGTGATTATCT
GAAGGCACTTTATCGCGATA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MMLKQTKIVASISDQRCEVEFIRDLFKAGMNVVRMNTAHASREGFEKLITNVREVSNRIAILMDTKGPEVRTTAIAGGEP
IPYQIGDKVKIVGNPAQETTRECIAVSYPGFVHDLQVDGDILIDDGDLELRVIEKTDEYLLCEVQNEATLGNRKSVNVPG
VRINLPSLTEKDRNNILYAIEKDIDFIAHSFVRNKQDVLDIRQILDAYGSDIKIIAKIENQEGVDNIDEILEVADGVMVA
RGDLGIEVPQERIPGIQRLLIKKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPVE
AVKTMAKIAAQAEKDKLAENDIRIPLDENSNDVTAFLAKQAVKATSKLNIRAIITDSFSGRTARNIAAFRGKYPVLAICY
KEKTMRHLALSYGVEAIYMPEKANGQAYYFAALRKLLDDGVLSESDMVAYLSSGKQGTQTSFLEINVVGDVLKYAMDYVL
PNRNRYL

Sequences:

>Translated_487_residues
MMLKQTKIVASISDQRCEVEFIRDLFKAGMNVVRMNTAHASREGFEKLITNVREVSNRIAILMDTKGPEVRTTAIAGGEP
IPYQIGDKVKIVGNPAQETTRECIAVSYPGFVHDLQVDGDILIDDGDLELRVIEKTDEYLLCEVQNEATLGNRKSVNVPG
VRINLPSLTEKDRNNILYAIEKDIDFIAHSFVRNKQDVLDIRQILDAYGSDIKIIAKIENQEGVDNIDEILEVADGVMVA
RGDLGIEVPQERIPGIQRLLIKKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPVE
AVKTMAKIAAQAEKDKLAENDIRIPLDENSNDVTAFLAKQAVKATSKLNIRAIITDSFSGRTARNIAAFRGKYPVLAICY
KEKTMRHLALSYGVEAIYMPEKANGQAYYFAALRKLLDDGVLSESDMVAYLSSGKQGTQTSFLEINVVGDVLKYAMDYVL
PNRNRYL
>Mature_487_residues
MMLKQTKIVASISDQRCEVEFIRDLFKAGMNVVRMNTAHASREGFEKLITNVREVSNRIAILMDTKGPEVRTTAIAGGEP
IPYQIGDKVKIVGNPAQETTRECIAVSYPGFVHDLQVDGDILIDDGDLELRVIEKTDEYLLCEVQNEATLGNRKSVNVPG
VRINLPSLTEKDRNNILYAIEKDIDFIAHSFVRNKQDVLDIRQILDAYGSDIKIIAKIENQEGVDNIDEILEVADGVMVA
RGDLGIEVPQERIPGIQRLLIKKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPVE
AVKTMAKIAAQAEKDKLAENDIRIPLDENSNDVTAFLAKQAVKATSKLNIRAIITDSFSGRTARNIAAFRGKYPVLAICY
KEKTMRHLALSYGVEAIYMPEKANGQAYYFAALRKLLDDGVLSESDMVAYLSSGKQGTQTSFLEINVVGDVLKYAMDYVL
PNRNRYL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=430, Percent_Identity=42.3255813953488, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI32967597, Length=430, Percent_Identity=42.3255813953488, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI33286422, Length=429, Percent_Identity=38.9277389277389, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI33286420, Length=429, Percent_Identity=38.9277389277389, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI33286418, Length=430, Percent_Identity=39.0697674418605, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=37.9790940766551, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=37.9790940766551, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=34.0425531914894, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=34.0425531914894, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.0467289719626, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1787965, Length=456, Percent_Identity=40.3508771929825, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI1788160, Length=456, Percent_Identity=37.280701754386, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17544584, Length=427, Percent_Identity=39.344262295082, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=37.7593360995851, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=37.7593360995851, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=37.7593360995851, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=37.7593360995851, Blast_Score=282, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6319279, Length=424, Percent_Identity=39.1509433962264, Blast_Score=269, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6324923, Length=433, Percent_Identity=38.3371824480369, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24648964, Length=477, Percent_Identity=36.8972746331237, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI28571814, Length=477, Percent_Identity=36.8972746331237, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24648966, Length=451, Percent_Identity=36.8070953436807, Blast_Score=275, Evalue=8e-74,
Organism=Drosophila melanogaster, GI24581235, Length=478, Percent_Identity=32.2175732217573, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24646914, Length=308, Percent_Identity=34.7402597402597, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 54239; Mature: 54239

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLKQTKIVASISDQRCEVEFIRDLFKAGMNVVRMNTAHASREGFEKLITNVREVSNRIA
CCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCEE
ILMDTKGPEVRTTAIAGGEPIPYQIGDKVKIVGNPAQETTRECIAVSYPGFVHDLQVDGD
EEEECCCCCEEEEEECCCCCCCEECCCEEEEECCCHHHHHHHEEEEECCCCEEEEEECCE
ILIDDGDLELRVIEKTDEYLLCEVQNEATLGNRKSVNVPGVRINLPSLTEKDRNNILYAI
EEEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEE
EKDIDFIAHSFVRNKQDVLDIRQILDAYGSDIKIIAKIENQEGVDNIDEILEVADGVMVA
ECHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEE
RGDLGIEVPQERIPGIQRLLIKKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIY
ECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
YRTDALMLSGETAYGKYPVEAVKTMAKIAAQAEKDKLAENDIRIPLDENSNDVTAFLAKQ
EEECEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
AVKATSKLNIRAIITDSFSGRTARNIAAFRGKYPVLAICYKEKTMRHLALSYGVEAIYMP
HHHHHHCCEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCEEEECC
EKANGQAYYFAALRKLLDDGVLSESDMVAYLSSGKQGTQTSFLEINVVGDVLKYAMDYVL
CCCCCCEEHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCEEEEEEEHHHHHHHHHHHHHC
PNRNRYL
CCCCCCC
>Mature Secondary Structure
MMLKQTKIVASISDQRCEVEFIRDLFKAGMNVVRMNTAHASREGFEKLITNVREVSNRIA
CCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCEE
ILMDTKGPEVRTTAIAGGEPIPYQIGDKVKIVGNPAQETTRECIAVSYPGFVHDLQVDGD
EEEECCCCCEEEEEECCCCCCCEECCCEEEEECCCHHHHHHHEEEEECCCCEEEEEECCE
ILIDDGDLELRVIEKTDEYLLCEVQNEATLGNRKSVNVPGVRINLPSLTEKDRNNILYAI
EEEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEE
EKDIDFIAHSFVRNKQDVLDIRQILDAYGSDIKIIAKIENQEGVDNIDEILEVADGVMVA
ECHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEE
RGDLGIEVPQERIPGIQRLLIKKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIY
ECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
YRTDALMLSGETAYGKYPVEAVKTMAKIAAQAEKDKLAENDIRIPLDENSNDVTAFLAKQ
EEECEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
AVKATSKLNIRAIITDSFSGRTARNIAAFRGKYPVLAICYKEKTMRHLALSYGVEAIYMP
HHHHHHCCEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCEEEECC
EKANGQAYYFAALRKLLDDGVLSESDMVAYLSSGKQGTQTSFLEINVVGDVLKYAMDYVL
CCCCCCEEHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCEEEEEEEHHHHHHHHHHHHHC
PNRNRYL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]