Click here to switch to the map view.

The map label for this gene is pitB [C]

Identifier: 148828411

GI number: 148828411

Start: 1864855

End: 1866117

Strand: Reverse

Name: pitB [C]

Synonym: CGSHiGG_10100

Alternate gene names: 148828411

Gene position: 1866117-1864855 (Counterclockwise)

Preceding gene: 148828412

Following gene: 148828410

Centisome position: 98.88

GC content: 39.9

Gene sequence:

>1263_bases
ATGGAAATTATTAGTCAATATGGTTCTTGGCTGGTGTGGATCACAGCAGTCTTTGGCTTTTTTATGGCGTTCGGAATTGG
AGCGAATGATGTCTCAAACTCAATGGGAACATCAGTAGGATCTGGTACCATAACCGCCAAACAAGCGATTATTATCGCAT
TAATTTTTGAATCTGCTGGTGCCTATCTAGCAGGTGGTGAAGTAACACAAACCATTAAAAGTGGTGTAATCGATCCAATA
CAATTTGTCGATACGCCAGATATTTTAGCTCTAGGTATGCTTTCAACCTTATTTGCATCGGGTGCTTGGTTATTTATTGC
AACCAAAATGGGTTGGCCAGTTTCTGGTACACACACTATTATTGGTGCAATTATTGGATTTGCCTGTATTACGATTGGCC
CTAGTTCTGTAGATTGGTCAAAGATTGGCAGCATCGTTGGTAGCTGGTTTGTCACGCCTGTCATTGCGGGTATTTTGGCT
TATGCAATCTTTGCAAGTACGCAAAAACTTATTTTTGATACCGAACAACCACTAAAAAATGCACAAAAATATGGCCCTTA
TTATATGGGGATAACCGTGTTTGTACTTTGTATCGTGACGATGAAAAAAGGCTTAAAACACGTAGGCTTAAATCTTTCAA
ACAGTGAAACTTTAATCATTTCACTTGCCATCAGTCTTATCGGAATGTTTTTCTTCCATTTCTATTTTAAAAGCAAAATC
TTCACTCAATCAGCAAACAAAGGCACTTTTGGTGCAGTAGAAAAAGTCTTCAGTATTTTAATGTTATTAACCGCTTGTGC
GATGGCATTTGCGCACGGTTCTAATGACGTAGCCAATGCAATTGGCCCTCTTTCTGCAGTGGTCTCTATTGTAAATGAAG
GCGGTAAAATTGTTTCAGGTGGAGCCTTAACTTGGTGGATTTTACCCTTAGGTGCATTAGGTATTGCTGTGGGCTTAATT
ACTATGGGACAAAAAGTGATGGCGACTGTTGGATCTGGGATCACCGATTTAACGCCAAGCCGTGGCTTTGCTGCTCAATT
TGCTACTGCAATGACTGTTGTAGTTGCATCAGGCACAGGCTTACCCATCTCAACAACACAAACACTTGTTGGAGCTATTT
TAGGTATCGGCTTTGCACGTGGTATCGCAGCACTGAATTTAACGGTTATTCGAAACATCATTAGTTCTTGGATTGTCACA
TTACCAGCAGGTGCATTTTTCGCCATCATTATTTTCTATGTGCTAAGAACTATCTTTAATTAA

Upstream 100 bases:

>100_bases
TGAATGGGTTGGCGTTTTGGCTGACCAAGCACAACGTGTCGGTTCGCGCATTGAATTAATGCTCGCGCGTTCATAATCAA
CAAAACATAGGAAATAAACT

Downstream 100 bases:

>100_bases
TTGATCTCAATAAAAAGTGCGGTTAATTTTAACCGCACTTTTGTTGTGATGTTTAAGGGGAAAAAATGAAAAAGATCTAT
AAAGCCTTAATCTCATCTTT

Product: phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIDPI
QFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTIIGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN

Sequences:

>Translated_420_residues
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIDPI
QFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTIIGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN
>Mature_420_residues
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIDPI
QFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTIIGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=250, Percent_Identity=34, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI31543630, Length=291, Percent_Identity=34.3642611683849, Blast_Score=150, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25146401, Length=463, Percent_Identity=33.0453563714903, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI71981576, Length=454, Percent_Identity=32.3788546255507, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17557328, Length=494, Percent_Identity=26.3157894736842, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17539280, Length=252, Percent_Identity=38.0952380952381, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32566716, Length=235, Percent_Identity=32.7659574468085, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17536725, Length=166, Percent_Identity=34.9397590361446, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319773, Length=242, Percent_Identity=35.1239669421488, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356511, Length=197, Percent_Identity=40.6091370558376, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 44012; Mature: 44012

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
AYLAGGEVTQTIKSGVIDPIQFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTI
CEEECCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
IGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILAYAIFASTQKLIFDTEQPLKN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
AQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GALTWWILPLGALGIAVGLITMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCC
LPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVTLPAGAFFAIIIFYVLRTIFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
AYLAGGEVTQTIKSGVIDPIQFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTI
CEEECCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
IGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILAYAIFASTQKLIFDTEQPLKN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
AQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GALTWWILPLGALGIAVGLITMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCC
LPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVTLPAGAFFAIIIFYVLRTIFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]