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The map label for this gene is hifB [H]

Identifier: 148828397

GI number: 148828397

Start: 1847861

End: 1848499

Strand: Direct

Name: hifB [H]

Synonym: CGSHiGG_10030

Alternate gene names: 148828397

Gene position: 1847861-1848499 (Clockwise)

Preceding gene: 148828395

Following gene: 148828398

Centisome position: 97.92

GC content: 43.04

Gene sequence:

>639_bases
GTGATTATCACTGGCACCAGAGTGATTTATCCCGCTGGGCAAAAAAATGTTATCGTGAAGTTAGAAAACAATGATGATTC
GGCTGCCTTGGTGCAAGCGTGGATTGATAATGGCAATCCAAATGCCGATCCAAAATACACCAAAACCCCTTTTGTGATTA
CCCCGCCTGTTGCTCGGGTGGAAGCGAAATCAGGGCAAAGTTTGCGGATTACGTTCACAGGCAGCGAGCCTTTACCTGAC
GATCGTGAAAGCCTCTTTTATTTTAATTTGTTAGATATACCGCCGAAACCCGATGCGGAATTTCTGGCAAAACACGGCAG
TTTTATGCAAATTGCTATTCGTTCACGTTTGAAGTTGTTTTATCGCCCTGCGAAACTTTCGCTTGACCCGTTTGATGCGA
TGAAAAAAGTGGTGTTTAAAGCCACCCCTAAAGGTGTGCTAGTGGATAATCAAACACCTTATTATATGAACTATATTGGT
TTGTTACATCAAAATAAACCTGCGAAAAATGTCAAAATGGTTGCGCCTTTTTCTCAAGCGGTATTTGAAGCTAAGGGGGT
ACGTTCTGGCGATAAATTGAAATGGGTGTTGGTTAATGATTATGGTGCCGACCAAGAAGGCGACGCCATCGCACAATAA

Upstream 100 bases:

>100_bases
TTAATTATGTTGAAATAGGGAAACAGAATGCTTAAAAAAACACGTTTATTTTTACCGCACTATTTTTACCGCACTTTGTG
CATTTTCAGCCAATGCGAAT

Downstream 100 bases:

>100_bases
ATTGCCGTGGGTGGGGGTAAGCCCACGTGGGACATAATCAAACAACTGTAAGATATTAGGAACGGTGTGCTAAAGCCTAC
GCTTGCACCCACTTTACAAT

Product: putative F17-like fimbrial chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIITGTRVIYPAGQKNVIVKLENNDDSAALVQAWIDNGNPNADPKYTKTPFVITPPVARVEAKSGQSLRITFTGSEPLPD
DRESLFYFNLLDIPPKPDAEFLAKHGSFMQIAIRSRLKLFYRPAKLSLDPFDAMKKVVFKATPKGVLVDNQTPYYMNYIG
LLHQNKPAKNVKMVAPFSQAVFEAKGVRSGDKLKWVLVNDYGADQEGDAIAQ

Sequences:

>Translated_212_residues
MIITGTRVIYPAGQKNVIVKLENNDDSAALVQAWIDNGNPNADPKYTKTPFVITPPVARVEAKSGQSLRITFTGSEPLPD
DRESLFYFNLLDIPPKPDAEFLAKHGSFMQIAIRSRLKLFYRPAKLSLDPFDAMKKVVFKATPKGVLVDNQTPYYMNYIG
LLHQNKPAKNVKMVAPFSQAVFEAKGVRSGDKLKWVLVNDYGADQEGDAIAQ
>Mature_212_residues
MIITGTRVIYPAGQKNVIVKLENNDDSAALVQAWIDNGNPNADPKYTKTPFVITPPVARVEAKSGQSLRITFTGSEPLPD
DRESLFYFNLLDIPPKPDAEFLAKHGSFMQIAIRSRLKLFYRPAKLSLDPFDAMKKVVFKATPKGVLVDNQTPYYMNYIG
LLHQNKPAKNVKMVAPFSQAVFEAKGVRSGDKLKWVLVNDYGADQEGDAIAQ

Specific function: Mediates assembly of pili by forming soluble multimeric complexes with pili subunits as an intermediate step in the assembly process. This protein is involved in type B pili (hifA) assembly [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=219, Percent_Identity=43.37899543379, Blast_Score=173, Evalue=8e-45,
Organism=Escherichia coli, GI1788428, Length=212, Percent_Identity=35.377358490566, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1790771, Length=200, Percent_Identity=35.5, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1789532, Length=207, Percent_Identity=34.2995169082126, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786743, Length=200, Percent_Identity=34, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786936, Length=206, Percent_Identity=32.5242718446602, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081806, Length=205, Percent_Identity=28.780487804878, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787171, Length=206, Percent_Identity=30.0970873786408, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788677, Length=207, Percent_Identity=31.4009661835749, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI87082203, Length=210, Percent_Identity=31.4285714285714, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 23587; Mature: 23587

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITGTRVIYPAGQKNVIVKLENNDDSAALVQAWIDNGNPNADPKYTKTPFVITPPVARV
CEEECCEEEEECCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCCEE
EAKSGQSLRITFTGSEPLPDDRESLFYFNLLDIPPKPDAEFLAKHGSFMQIAIRSRLKLF
ECCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCEEEEHHHHHHHEE
YRPAKLSLDPFDAMKKVVFKATPKGVLVDNQTPYYMNYIGLLHQNKPAKNVKMVAPFSQA
ECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHEEEEEEEECCCCCCCEEEECCHHHH
VFEAKGVRSGDKLKWVLVNDYGADQEGDAIAQ
HHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MIITGTRVIYPAGQKNVIVKLENNDDSAALVQAWIDNGNPNADPKYTKTPFVITPPVARV
CEEECCEEEEECCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCCEE
EAKSGQSLRITFTGSEPLPDDRESLFYFNLLDIPPKPDAEFLAKHGSFMQIAIRSRLKLF
ECCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCEEEEHHHHHHHEE
YRPAKLSLDPFDAMKKVVFKATPKGVLVDNQTPYYMNYIGLLHQNKPAKNVKMVAPFSQA
ECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHEEEEEEEECCCCCCCEEEECCHHHH
VFEAKGVRSGDKLKWVLVNDYGADQEGDAIAQ
HHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7997179 [H]