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The map label for this gene is thiB [H]

Identifier: 148828187

GI number: 148828187

Start: 1611859

End: 1612857

Strand: Direct

Name: thiB [H]

Synonym: CGSHiGG_08705

Alternate gene names: 148828187

Gene position: 1611859-1612857 (Clockwise)

Preceding gene: 148828185

Following gene: 148828188

Centisome position: 85.41

GC content: 38.24

Gene sequence:

>999_bases
ATGAAACTTCTTAAATTAACCTTAATTTCTACCGCACTTTTTTCCACTGCGGCATTAGCTCAAGCACAGCAAAGTGTAAA
CGTATATAGCTACGACTCTTTCATTTCTGAATGGGGGGCTGGGCCAAAAGTAAAACAAGATTTTGAAAAAGCCCATCCAC
AATGTGCGATTAATTTTACGCCTTTTGAAAGCGTTGGTGTGTTGCTCAACCGTGTTCGTTTGGAAGGAAAGAAAACAAAA
GCGGATATTGTGCTAGGTTTAGATAACTTTTTTTTGGAACAAGCTGAAAAAACAGGTATTTTTGCGCCAAATAACGTGGA
TTTAACTCAGCTGGATTTACCAACAAAATGGGCGAATAAAACCTTCTTACCTTTTGATTTCGGTAATTATGCTTTTGTGT
ACGATAAAACTAAATTACAAAACCCGCCGAAAAGTTTGAAAGAATTAGTTGAACGTCAAGATCTTAGTGTGATTTATCAA
GATCCTCGCACCAGCAGTGTGGGTCGTGGTTTATTGGTTTGGATGAATGCTGTTTACCCTGCGGATAAAATTCAATCCGC
TTGGAAAGAGTTAGATAAGCATACAGTGACAGTGGGTAAAGGCTGGTCAGATACTTACGGCGCATTCTTAAAAGGCGAAG
CGGATTTAGTATTAAGTTACAGCACATCGCCACTTTATCATCAACTTTTTGAGAAAAAAGATAATTATGCGGCGACAGAT
TTTGCGGAGGGTCATATTACTCAAGTAGAATTAGCGGCGCGAGTTGCAAACCATCCAAATCAATGTGCGGATGATTTTAT
GGCATTTTTAATTTCGCCTACAGCACAAAAACATATTGTGACCGCGAACATTATGTTGCCAGTTATTCAAGGCGAAATTG
AACCGCACTTTGATGCGCTTAAAGTTCAACAAAAAACTCAAACGTCAATTAATCCAACAATGAATGCTGAGCAGTTAAAA
AATTGGATCAGCACTTGGCAAACTACATTAACAAAATAA

Upstream 100 bases:

>100_bases
GAACCTGAAACAGTTAATACTGACGTAGGAAACTAGGAATATAACCTTATCTCCTTTTCGTCATTATTGCTGCCTTTTAA
TCATAAATAAGGATGTAATA

Downstream 100 bases:

>100_bases
TTTGATGTTTTCGTTATTTCATCATCCACAACTTCGCCCTCGTCATTATGCGGGCGGGGTTGTTGTCATTAGTTTCATTA
TTCTTTTTTATGGTAGCGCC

Product: putative aldolase

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFISEWGAGPKVKQDFEKAHPQCAINFTPFESVGVLLNRVRLEGKKTK
ADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANKTFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQ
DPRTSSVGRGLLVWMNAVYPADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDALKVQQKTQTSINPTMNAEQLK
NWISTWQTTLTK

Sequences:

>Translated_332_residues
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFISEWGAGPKVKQDFEKAHPQCAINFTPFESVGVLLNRVRLEGKKTK
ADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANKTFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQ
DPRTSSVGRGLLVWMNAVYPADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDALKVQQKTQTSINPTMNAEQLK
NWISTWQTTLTK
>Mature_332_residues
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFISEWGAGPKVKQDFEKAHPQCAINFTPFESVGVLLNRVRLEGKKTK
ADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANKTFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQ
DPRTSSVGRGLLVWMNAVYPADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDALKVQQKTQTSINPTMNAEQLK
NWISTWQTTLTK

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=313, Percent_Identity=52.0766773162939, Blast_Score=310, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37257; Mature: 37257

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFISEWGAGPKVKQDFEKAHPQCAINFT
CCEEHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCHHHHHHHCCCCEEEECC
PFESVGVLLNRVRLEGKKTKADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANK
CHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCEEEECCCCHHCCC
TFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQDPRTSSVGRGLLVWMNAVYP
EECEEECCCEEEEEECHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHEEHHHHCCC
ADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
HHHHHHHHHHHHHCEEEECCCCCCCCCCEECCCCEEEEEECCCHHHHHHHHCCCCCCCCC
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDAL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCHHHH
KVQQKTQTSINPTMNAEQLKNWISTWQTTLTK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFISEWGAGPKVKQDFEKAHPQCAINFT
CCEEHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCHHHHHHHCCCCEEEECC
PFESVGVLLNRVRLEGKKTKADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANK
CHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCEEEECCCCHHCCC
TFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQDPRTSSVGRGLLVWMNAVYP
EECEEECCCEEEEEECHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHEEHHHHCCC
ADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
HHHHHHHHHHHHHCEEEECCCCCCCCCCEECCCCEEEEEECCCHHHHHHHHCCCCCCCCC
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDAL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCHHHH
KVQQKTQTSINPTMNAEQLKNWISTWQTTLTK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]