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The map label for this gene is glpF [C]

Identifier: 148828185

GI number: 148828185

Start: 1610373

End: 1611086

Strand: Direct

Name: glpF [C]

Synonym: CGSHiGG_08695

Alternate gene names: 148828185

Gene position: 1610373-1611086 (Clockwise)

Preceding gene: 148828184

Following gene: 148828187

Centisome position: 85.33

GC content: 39.36

Gene sequence:

>714_bases
ATGGAAAATATCAATCTGTATCTTGGTGAATTAGTAGGAACTGCATTCTTTATGGTATTAGGGTTAGGTGTATGCGCTAA
TATTTCGCTAAAAAAATCAGGAATGTATGGCAGTGGCGGTTTACTAGCAGCTTGCGGCTGGGGGCTTTCAATGGTGTCAA
TTGCTGTGATTTTTGGATCTTTAACTGGTGCACATGTTAATCCTGCTGTAACAATTGGTTTTTGGGCTGTAGGGCAGTTT
TCAGGTGAATTAGTGTTCGGTTATATTGTTTCACAATGTATTGGGGCATTCATCGGGGCATTGATTGTTTGGCAATTATT
CAAGGATCATTTAGATGAAGAAGAAAATCAAAATTGTCAATTAGGTTCTTTTGCCACTATTGCAACAAACTCTAATAACT
TACGAAATTTATTTTCTGAAATAGTGGCTACATTCTCTCTTTTGTTTATATTGTTTGCTTTAGGTCATCAACAACCAGCT
AAAGGGGTGGCAATGTTCTTTGTGTTTGCAGGTGTAGCTGGGGGCGTGATGTCCTTTGGCGGTTTAACTGGTTATGCTAT
TAATCCAGCGCGTGATTTTATGCCGCGTCTTGTTCACGCGATTGTGCCAATCAAAAATAAAGGCAGCTCCAATTTTGGTT
ACGCTTGGGTTCCTATTTTCGGACCGATTATTGGCGCATTGTTAGCTGCTTGGTTATACAAAGCATTGTTTTAG

Upstream 100 bases:

>100_bases
CAAGCCAAGTGACTTGCATTGCTGAAGTTGAATAAAGCATTTGGAGTGCTAAAGGATACTCTCTAGCACTCTTTTTGTAT
TTAGATGGGAGATGGTTACC

Downstream 100 bases:

>100_bases
TTTTAATGTGGGGAAGATAAGGATGTAGTTAAATGTGTTATAGTTTTGGGGCTGACGTAGATTAGCCCTAAATACCACTC
CATTTTCGCAATGTTTTAAG

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MENINLYLGELVGTAFFMVLGLGVCANISLKKSGMYGSGGLLAACGWGLSMVSIAVIFGSLTGAHVNPAVTIGFWAVGQF
SGELVFGYIVSQCIGAFIGALIVWQLFKDHLDEEENQNCQLGSFATIATNSNNLRNLFSEIVATFSLLFILFALGHQQPA
KGVAMFFVFAGVAGGVMSFGGLTGYAINPARDFMPRLVHAIVPIKNKGSSNFGYAWVPIFGPIIGALLAAWLYKALF

Sequences:

>Translated_237_residues
MENINLYLGELVGTAFFMVLGLGVCANISLKKSGMYGSGGLLAACGWGLSMVSIAVIFGSLTGAHVNPAVTIGFWAVGQF
SGELVFGYIVSQCIGAFIGALIVWQLFKDHLDEEENQNCQLGSFATIATNSNNLRNLFSEIVATFSLLFILFALGHQQPA
KGVAMFFVFAGVAGGVMSFGGLTGYAINPARDFMPRLVHAIVPIKNKGSSNFGYAWVPIFGPIIGALLAAWLYKALF
>Mature_237_residues
MENINLYLGELVGTAFFMVLGLGVCANISLKKSGMYGSGGLLAACGWGLSMVSIAVIFGSLTGAHVNPAVTIGFWAVGQF
SGELVFGYIVSQCIGAFIGALIVWQLFKDHLDEEENQNCQLGSFATIATNSNNLRNLFSEIVATFSLLFILFALGHQQPA
KGVAMFFVFAGVAGGVMSFGGLTGYAINPARDFMPRLVHAIVPIKNKGSSNFGYAWVPIFGPIIGALLAAWLYKALF

Specific function: Transporter Of Glycerol Across The Cytoplasmic Membrane, With Limited Permeability To Water And Small Uncharged Compounds Such As Polyols. [C]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=246, Percent_Identity=31.3008130081301, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI157266307, Length=244, Percent_Identity=29.5081967213115, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4502187, Length=246, Percent_Identity=30.8943089430894, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502181, Length=233, Percent_Identity=27.8969957081545, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4755125, Length=233, Percent_Identity=27.8969957081545, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37694062, Length=190, Percent_Identity=28.9473684210526, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1790362, Length=246, Percent_Identity=32.520325203252, Blast_Score=94, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=243, Percent_Identity=30.8641975308642, Blast_Score=90, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17531431, Length=243, Percent_Identity=30.8641975308642, Blast_Score=90, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17544068, Length=247, Percent_Identity=33.6032388663968, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71992966, Length=240, Percent_Identity=30.4166666666667, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17558372, Length=214, Percent_Identity=28.5046728971963, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994009, Length=236, Percent_Identity=27.9661016949153, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17533613, Length=201, Percent_Identity=27.363184079602, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32564052, Length=201, Percent_Identity=27.363184079602, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71993722, Length=203, Percent_Identity=26.6009852216749, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652747, Length=235, Percent_Identity=33.1914893617021, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45551084, Length=235, Percent_Identity=33.1914893617021, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45550503, Length=242, Percent_Identity=25.2066115702479, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25180; Mature: 25180

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENINLYLGELVGTAFFMVLGLGVCANISLKKSGMYGSGGLLAACGWGLSMVSIAVIFGS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LTGAHVNPAVTIGFWAVGQFSGELVFGYIVSQCIGAFIGALIVWQLFKDHLDEEENQNCQ
CCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LGSFATIATNSNNLRNLFSEIVATFSLLFILFALGHQQPAKGVAMFFVFAGVAGGVMSFG
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
GLTGYAINPARDFMPRLVHAIVPIKNKGSSNFGYAWVPIFGPIIGALLAAWLYKALF
CCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MENINLYLGELVGTAFFMVLGLGVCANISLKKSGMYGSGGLLAACGWGLSMVSIAVIFGS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LTGAHVNPAVTIGFWAVGQFSGELVFGYIVSQCIGAFIGALIVWQLFKDHLDEEENQNCQ
CCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LGSFATIATNSNNLRNLFSEIVATFSLLFILFALGHQQPAKGVAMFFVFAGVAGGVMSFG
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
GLTGYAINPARDFMPRLVHAIVPIKNKGSSNFGYAWVPIFGPIIGALLAAWLYKALF
CCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]