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The map label for this gene is pfkA [H]

Identifier: 148828157

GI number: 148828157

Start: 1567246

End: 1568163

Strand: Reverse

Name: pfkA [H]

Synonym: CGSHiGG_08485

Alternate gene names: 148828157

Gene position: 1568163-1567246 (Counterclockwise)

Preceding gene: 148828158

Following gene: 148828156

Centisome position: 83.1

GC content: 41.61

Gene sequence:

>918_bases
ATGAATGCCGCAATTCGTGGCGTAGTACGTTCTGCACTTGCAGAAGGCTTGGAAGTGTTTGGTATCTATGATGGTTACCA
AGGTTTATACAATAATAAAATCAAACAATTAAATCGCTATAGCGTATCTGATGTCATTAATCGTGGCGGTACTTTTTTAG
GCTCTGCACGTTTTCCTGAATTTAAAGATCCTAATATTCGGGCAAAATGTGCGGAAATTTTACGTTCTCACGGCATTGAT
GCACTTGTGGTTATCGGTGGTGACGGTTCTTATATGGGGGCAAAATTATTAACCGAAGAACACTGTTTCCCTTGTGTTGG
ATTACCCGGTACTATTGATAATGATGTGGCAGGTACTGACTATACCATCGGTTATCAAACCGCATTACAAACTGCAGTGG
ATGCAATCGACCGTTTACGTGATACATCAAGCTCACACCAACGTATTTCTATCGTTGAAATTATGGGTCGCCATTGTAGT
GACTTAACGATTTCTGCGGGGATCGCTGGCGGTTGCGAATATATCGTCGCATCAGAAATTGAATTTAATCGCGAAGAATT
AATTCAACAAATTGAACGTAGCATTATTCGCGGTAAACGCCACGCTATTATTGCTATCACTGAACTTTTAACTGATGTTC
ATTCTCTGGCAAAAGAAATTGAAGCTCGCGTAGGCCACGAAACTCGCGCAACCGTTTTAGGGCATATTCAACGTGGTGGT
TCTCCTTGTGCTTTTGACCGTATTCTTGCCTCACGTATGGGCGCATACGCAGTAGATTTATTGCTACAAGGCAAGGGCGG
CTATTGCGTAGGTATCCAAAATGAACAGCTCGTTCATCACGATATTATTGATGCCATTAATAATATGCAGCGTGTATTTA
AGGCTGATTGGCTTAAAGTGGCAAAACGTTTGGAATAA

Upstream 100 bases:

>100_bases
AATGATGTAATCTTATGCCCATTTTCAATTATGGTTAAAAGTAGGTAAATTATGATTAAAAAAAATTGCAGTTTTAACAA
GTGGTGGCGATGCGCCAGGT

Downstream 100 bases:

>100_bases
TCCTTTATTCACTATATAAATCACTAACACTTAATTCATCTTTTTATCTTTCTCAAACAGACACAACAATGTGTCTGTTT
TTTCTTAAAAAGTGCGGTCA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGTFLGSARFPEFKDPNIRAKCAEILRSHGID
ALVVIGGDGSYMGAKLLTEEHCFPCVGLPGTIDNDVAGTDYTIGYQTALQTAVDAIDRLRDTSSSHQRISIVEIMGRHCS
DLTISAGIAGGCEYIVASEIEFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARVGHETRATVLGHIQRGG
SPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIIDAINNMQRVFKADWLKVAKRLE

Sequences:

>Translated_305_residues
MNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGTFLGSARFPEFKDPNIRAKCAEILRSHGID
ALVVIGGDGSYMGAKLLTEEHCFPCVGLPGTIDNDVAGTDYTIGYQTALQTAVDAIDRLRDTSSSHQRISIVEIMGRHCS
DLTISAGIAGGCEYIVASEIEFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARVGHETRATVLGHIQRGG
SPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIIDAINNMQRVFKADWLKVAKRLE
>Mature_305_residues
MNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGTFLGSARFPEFKDPNIRAKCAEILRSHGID
ALVVIGGDGSYMGAKLLTEEHCFPCVGLPGTIDNDVAGTDYTIGYQTALQTAVDAIDRLRDTSSSHQRISIVEIMGRHCS
DLTISAGIAGGCEYIVASEIEFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARVGHETRATVLGHIQRGG
SPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIIDAINNMQRVFKADWLKVAKRLE

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=332, Percent_Identity=36.144578313253, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI266453748, Length=332, Percent_Identity=36.144578313253, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI4505749, Length=332, Percent_Identity=36.144578313253, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI266453619, Length=332, Percent_Identity=36.144578313253, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI11321601, Length=332, Percent_Identity=35.8433734939759, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI48762920, Length=332, Percent_Identity=37.9518072289157, Blast_Score=185, Evalue=4e-47,
Organism=Escherichia coli, GI1790350, Length=304, Percent_Identity=73.3552631578947, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25147584, Length=338, Percent_Identity=35.7988165680473, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17558788, Length=297, Percent_Identity=38.3838383838384, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI72003601, Length=279, Percent_Identity=32.258064516129, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321679, Length=333, Percent_Identity=35.7357357357357, Blast_Score=176, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323861, Length=346, Percent_Identity=33.8150289017341, Blast_Score=162, Evalue=5e-41,
Organism=Drosophila melanogaster, GI28573326, Length=332, Percent_Identity=38.855421686747, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24652337, Length=332, Percent_Identity=38.2530120481928, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI17647809, Length=332, Percent_Identity=38.2530120481928, Blast_Score=197, Evalue=9e-51,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 33399; Mature: 33399

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGTFLGSARFPE
CCHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
FKDPNIRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHCFPCVGLPGTIDNDVAGTD
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
YTIGYQTALQTAVDAIDRLRDTSSSHQRISIVEIMGRHCSDLTISAGIAGGCEYIVASEI
EECHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCEEECCCCCCCCEEEECCC
EFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARVGHETRATVLGHIQRGG
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
SPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIIDAINNMQRVFKADWLKV
CCHHHHHHHHHHHHHHEEHHEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKRLE
HHHCC
>Mature Secondary Structure
MNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGTFLGSARFPE
CCHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
FKDPNIRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHCFPCVGLPGTIDNDVAGTD
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
YTIGYQTALQTAVDAIDRLRDTSSSHQRISIVEIMGRHCSDLTISAGIAGGCEYIVASEI
EECHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCEEECCCCCCCCEEEECCC
EFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARVGHETRATVLGHIQRGG
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
SPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIIDAINNMQRVFKADWLKV
CCHHHHHHHHHHHHHHEEHHEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKRLE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA