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The map label for this gene is smpB [H]

Identifier: 148828156

GI number: 148828156

Start: 1566522

End: 1567007

Strand: Reverse

Name: smpB [H]

Synonym: CGSHiGG_08480

Alternate gene names: 148828156

Gene position: 1567007-1566522 (Counterclockwise)

Preceding gene: 148828157

Following gene: 148828144

Centisome position: 83.03

GC content: 35.8

Gene sequence:

>486_bases
ATGACAAAGAAAAAAGTAAAACCCAATTCAAATACTATCGCACTGAATAAACGTGCAAGACACGATTATTTTATTGAAGA
TGAAATTGAAGCAGGTCTTGAATTACAAGGCTGGGAAGTCAAATCTATACGCGCAGGCAAGGCAAATATTAGTGATAGTT
ATGTTATTTTTAAAAATGGCGAAGCCTTTTTATTCGGCGCAAGCATTCAGCCATTAAATGTTGCATCAACGCATATTGTT
TGTGATCCAACTCGCACTCGTAAGTTATTGTTAAATAAACGCGAATTAGCATCCCTATTTGGCAAAGCAAACCGAGACGG
TTTTACCATAGTTGCACTTTCTCTTTACTGGAAAAGTGCGTGGGCAAAAGTCAAAATTGGTTTAGCCAAAGGTAAAAAAC
AACAGGATAAACGTGATGATATTAAAGAACGTGAATGGAAAGTAACAAAAGATCGCATTATGAAAAATGCACATCGAAGA
TCTTAA

Upstream 100 bases:

>100_bases
GATTGAAAATAAATGCGACTGCCAGTTCTACCTAGATGATCTTTTTGCTATCAAAATAGAGATCCGATAAAATGCCTCAG
GCAAAATAAGGGATAAAATT

Downstream 100 bases:

>100_bases
ATTGTAATAATAAATTAGCCCCAAATTACTTGGGGCTAATTTTTAAAATAGAATGAAAAACATTCAATCTTTTTGTTAAT
AAAAGAATATCGCCTTATCC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSIRAGKANISDSYVIFKNGEAFLFGASIQPLNVASTHIV
CDPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSAWAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRR
S

Sequences:

>Translated_161_residues
MTKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSIRAGKANISDSYVIFKNGEAFLFGASIQPLNVASTHIV
CDPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSAWAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRR
S
>Mature_160_residues
TKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSIRAGKANISDSYVIFKNGEAFLFGASIQPLNVASTHIVC
DPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSAWAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRRS

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=78.75, Blast_Score=267, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18416; Mature: 18284

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSIRAGKANISDSYVIFKNG
CCCCCCCCCCCEEEEECCCCCCCEECCHHCCCCEECCEEEEEEECCCCCCCCCEEEEECC
EAFLFGASIQPLNVASTHIVCDPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSA
CEEEEECCCCCCCCCCEEEEECCCHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEEEHHCC
WAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRRS
CEEEEEEECCCCCCCCHHCCHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSIRAGKANISDSYVIFKNG
CCCCCCCCCCEEEEECCCCCCCEECCHHCCCCEECCEEEEEEECCCCCCCCCEEEEECC
EAFLFGASIQPLNVASTHIVCDPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSA
CEEEEECCCCCCCCCCEEEEECCCHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEEEHHCC
WAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRRS
CEEEEEEECCCCCCCCHHCCHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA