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The map label for this gene is prmA [H]

Identifier: 148828154

GI number: 148828154

Start: 1565154

End: 1566134

Strand: Direct

Name: prmA [H]

Synonym: CGSHiGG_08470

Alternate gene names: 148828154

Gene position: 1565154-1566134 (Clockwise)

Preceding gene: 148828153

Following gene: 148828155

Centisome position: 82.94

GC content: 40.77

Gene sequence:

>981_bases
ATGCGAATCGGTTCATACCAATTAAAAAATCGTGTTTTACTTGCACCAATGGCAGGTATTACCGACCAGCCTTTCCGCCG
TCTTTGTGCATATTATGGTGCGGGGCTTACATTTTCAGAAATGATGTCCACAAATCCGCAAGTTTGGCATACCGAAAAAT
CCAAATTGCGTTTGGCACATAGCGAAGATTTAGGTCTCAATGCGGTGCAAATTGCAGGTAGTGATCCACTCGAGATGGCG
CAAGCCGCTGCTATTAACGTGGAATATGGCGCTCAAATCATTGACATTAATATGGGATGCCCTGCCAAAAAAGTAAATCG
CAAACTCGCAGGCTCTGCGTTGTTGCAATTTCCTTATTTGGTGGAAAAAATCTTGCGAGAAGTAGTGTCCGCCGTCAATG
TGCCAGTGACCTTAAAAATTCGTACAGGCTGGGATAAATCTAATCGAAATTGCGTTCAAATCGGCAAAATTGCAGAACAA
TGTGGCATACAAGCATTAACGGTGCATGGTCGTACACGCGCTTGTCTTTTTGAAGGTGAAGCGGAGTACGATAACATTAA
AGCCGTTAAACAAGCGATTGCTATACCAGTTATAGCTAATGGCGATATTGATTCTGCTCGGAAAGCAAAATTCGTTCTTG
ATTATACTGGAGCGGATGCCATTATGATCGGTCGTGCTGCCTTAGGTAACCCTTGGTTATTTCAAGCGGTAGAAAACCTA
ATTGAGCATAACTCGATAAGTCAAATGCCAAGTTTAAAAGAAAAGTGTGGTCAGATTTTGCGTCATATTCAAGAATTACA
TCAATTCTATGGCGAACAAAAAGGCTATCGCATTGCGCGTAAACACGTAGCTTGGTATTTACAGGGAATTCAACCTGATT
CCGTTTTTAAACAGACTTTTAATGCAATTAGTGATCCAAAAGAGCAGCTGATTGTATTGGAAGATTTTTTCAATTTAATT
TTGGATAAAGAAAAATGTTAG

Upstream 100 bases:

>100_bases
CTTTGCAAAGCAAGGCAAAATGCGTATTATAGCCAGCCTTGTCGGTTGTCGAGCAGTTCGATATAAATTAGATTTAGGCT
TGCTAGGAAAGGAACGTAAA

Downstream 100 bases:

>100_bases
AACAACAACGTAATTCTGCCGATGCCTTAACGGTATCAGTATTAAATGCGCAATCACAAGTGACCAGCAAACCATTACGC
GATTCAGTCAAACAAGCGTT

Product: ribosomal protein L11 methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRIGSYQLKNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAHSEDLGLNAVQIAGSDPLEMA
QAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPYLVEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQ
CGIQALTVHGRTRACLFEGEAEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLDYTGADAIMIGRAALGNPWLFQAVENL
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTFNAISDPKEQLIVLEDFFNLI
LDKEKC

Sequences:

>Translated_326_residues
MRIGSYQLKNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAHSEDLGLNAVQIAGSDPLEMA
QAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPYLVEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQ
CGIQALTVHGRTRACLFEGEAEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLDYTGADAIMIGRAALGNPWLFQAVENL
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTFNAISDPKEQLIVLEDFFNLI
LDKEKC
>Mature_326_residues
MRIGSYQLKNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAHSEDLGLNAVQIAGSDPLEMA
QAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPYLVEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQ
CGIQALTVHGRTRACLFEGEAEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLDYTGADAIMIGRAALGNPWLFQAVENL
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTFNAISDPKEQLIVLEDFFNLI
LDKEKC

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=237, Percent_Identity=32.9113924050633, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI239788462, Length=237, Percent_Identity=32.9113924050633, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI31742496, Length=229, Percent_Identity=33.6244541484716, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=33.0677290836653, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=33.4763948497854, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=65.2996845425868, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1788462, Length=239, Percent_Identity=36.8200836820084, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI145693211, Length=248, Percent_Identity=28.6290322580645, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=221, Percent_Identity=33.4841628959276, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=245, Percent_Identity=31.8367346938775, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=32.9268292682927, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=226, Percent_Identity=30.9734513274336, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=315, Percent_Identity=24.4444444444444, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=263, Percent_Identity=27.7566539923954, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585320, Length=244, Percent_Identity=31.5573770491803, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24580595, Length=234, Percent_Identity=34.1880341880342, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19920448, Length=234, Percent_Identity=34.1880341880342, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=30.8370044052863, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45549423, Length=293, Percent_Identity=29.6928327645051, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36346; Mature: 36346

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGSYQLKNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAH
CCCCCHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEECCHHHEEEEC
SEDLGLNAVQIAGSDPLEMAQAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPYL
CCCCCCEEEEECCCCHHHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQCGIQALTVHGRTRACLFEGE
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLDYTGADAIMIGRAALGNPWLFQAVENL
CCHHHHHHHHHHHHCEEEECCCCCCCCCCEEEEEECCCCEEEECCHHCCCCHHHHHHHHH
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTF
HHCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
NAISDPKEQLIVLEDFFNLILDKEKC
HHHCCHHHHEEEHHHHHHHHHCCCCC
>Mature Secondary Structure
MRIGSYQLKNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAH
CCCCCHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEECCHHHEEEEC
SEDLGLNAVQIAGSDPLEMAQAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPYL
CCCCCCEEEEECCCCHHHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQCGIQALTVHGRTRACLFEGE
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLDYTGADAIMIGRAALGNPWLFQAVENL
CCHHHHHHHHHHHHCEEEECCCCCCCCCCEEEEEECCCCEEEECCHHCCCCHHHHHHHHH
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTF
HHCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
NAISDPKEQLIVLEDFFNLILDKEKC
HHHCCHHHHEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]