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The map label for this gene is proB [H]

Identifier: 148828080

GI number: 148828080

Start: 1487292

End: 1488398

Strand: Direct

Name: proB [H]

Synonym: CGSHiGG_08060

Alternate gene names: 148828080

Gene position: 1487292-1488398 (Clockwise)

Preceding gene: 148828077

Following gene: 148828081

Centisome position: 78.81

GC content: 39.93

Gene sequence:

>1107_bases
ATGAACAAAAAAACAATCGTTGTGAAATTCGGTACAAGCACGCTAACTCAGGGTTCGCCAAAACTGAATTCGCCACACAT
GATGGAAATCGTTCGTCAAATTGCACAACTGCATAATGATGGATTTCGTATAGTGATCGTGACTTCTGGTGCGATTGCAG
CGGGTCGCCATTATTTAAATCATCCTCAATTACCACCCACTATCGCTTCAAAGCAACTGCTTGCAGCGGTTGGTCAGAGC
CAATTAATTCAGGCTTGGGAAAAATTATTTGCTATTTATGATATCCATATCGGGCAACTTTTATTAACCCGTGCAGATAT
TGAAGATCGCGAACGTTTTTTAAATGCGCGAGATACTTTACACGCACTTTTAGATAACCACATTATTCCTGTGATTAATG
AAAATGACGCGGTGGCGACTGCGGAAATTAAAGTAGGTGACAATGACAATTTATCGGCATTAGTCGCTATTCTTGTGCAA
GCGGAACAACTTTATTTATTGACTGATCAACAAGGTTTATTTGATAGTGATCCGCGTAAAAATTCTGAAGCGAAATTAAT
TCCAGTTGTAGAACAAATTACCGATCATATTCGATCAATTGCAGGAGGAAGCGGTACAAATCTTGGTACTGGTGGAATGA
TGACCAAAATCATTGCTGCCGATGTTGCGACTCGCTCTGGAATTGAAACCATTATTGCTCCAGGTAATCGCCCAAATGTA
ATTGCAGATCTTGCTTATGAACAAAATATTGGTACAAAATTTATTGCACACCAATCTGATCGATTAGAAAGCCGCAAACA
ATGGCTTTTCGCCGCACCATCGGCAGGTATTATTACCATTGATAACGGTGCGCAAAATGCCATTCTTGAACAAAATAAAT
CTTTATTACCCGCTGGTATTATTAACGTGGAAGGGCGTTTTTCGCGTGGTGAAGTAGTCAAAATACGCACTCAATCAGGT
AAAGACATTGCGCTGGGTATGCCACGCTATAACAGCGATTCCCTACAATTAATTAAGGGCAGAAAATCAGCAGACATTGA
GAATGTTTTAGGTTATGAATATGGCGCAGTGGCAATGCATCGTGATGATATGATTATTTTGTCATAA

Upstream 100 bases:

>100_bases
AAATCCCCCTAAAATTTGCCGTTACTATACCTGATATTAAGTGATTTTTCATTGATTTAGTGATAACGTTAATGCCAATT
TTAAGAACTTGGAAAGGACT

Downstream 100 bases:

>100_bases
TGTCCTTTGGTTGATAAAAGCACTGATTTATGAAACAATCTTGAGCTATTTTTATAGGAAAAATTTAAGGTAAAACGATA
GTGATCGATTTTGATGGCTA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQS
QLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTLHALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQ
AEQLYLLTDQQGLFDSDPRKNSEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGIINVEGRFSRGEVVKIRTQSG
KDIALGMPRYNSDSLQLIKGRKSADIENVLGYEYGAVAMHRDDMIILS

Sequences:

>Translated_368_residues
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQS
QLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTLHALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQ
AEQLYLLTDQQGLFDSDPRKNSEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGIINVEGRFSRGEVVKIRTQSG
KDIALGMPRYNSDSLQLIKGRKSADIENVLGYEYGAVAMHRDDMIILS
>Mature_368_residues
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQS
QLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTLHALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQ
AEQLYLLTDQQGLFDSDPRKNSEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGIINVEGRFSRGEVVKIRTQSG
KDIALGMPRYNSDSLQLIKGRKSADIENVLGYEYGAVAMHRDDMIILS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=284, Percent_Identity=33.8028169014084, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=33.5664335664336, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=65.3846153846154, Blast_Score=472, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17569969, Length=266, Percent_Identity=31.9548872180451, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17569967, Length=266, Percent_Identity=31.9548872180451, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=36.144578313253, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=35.7487922705314, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI21357643, Length=284, Percent_Identity=31.6901408450704, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40115; Mature: 40115

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLN
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHCHHHCC
HPQLPPTIASKQLLAAVGQSQLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTL
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
HALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQAEQLYLLTDQQGLFDSDPRK
HHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCC
NSEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
CCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCE
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGI
EEEEEECCCCCCEEEECCHHHHHHHHCEEEECCCCCEEEECCCCCHHHHHCCCCCCCEEE
INVEGRFSRGEVVKIRTQSGKDIALGMPRYNSDSLQLIKGRKSADIENVLGYEYGAVAMH
EEECCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHEECCCCCCCHHHHHCCCCCEEEEE
RDDMIILS
CCCEEEEC
>Mature Secondary Structure
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLN
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHCHHHCC
HPQLPPTIASKQLLAAVGQSQLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTL
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
HALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQAEQLYLLTDQQGLFDSDPRK
HHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCC
NSEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
CCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCE
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGI
EEEEEECCCCCCEEEECCHHHHHHHHCEEEECCCCCEEEECCCCCHHHHHCCCCCCCEEE
INVEGRFSRGEVVKIRTQSGKDIALGMPRYNSDSLQLIKGRKSADIENVLGYEYGAVAMH
EEECCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHEECCCCCCCHHHHHCCCCCEEEEE
RDDMIILS
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA