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The map label for this gene is ispB [H]

Identifier: 148828062

GI number: 148828062

Start: 1464021

End: 1464992

Strand: Direct

Name: ispB [H]

Synonym: CGSHiGG_07950

Alternate gene names: 148828062

Gene position: 1464021-1464992 (Clockwise)

Preceding gene: 148828058

Following gene: 148828065

Centisome position: 77.58

GC content: 40.95

Gene sequence:

>972_bases
ATGAGCATAGACGAGATCCAGAAACTTGCAGATCCAGATATGCAAAAAGTTAATCAAAATATTCTTGCTCAGCTTAATTC
AGATGTACCGCTAATAGGACAGTTAGGTTTTTATATTGTTCAAGGTGGTGGTAAGCGGATCCGCCCGTTGATCGCAGTTC
TCGCAGCACGATCTTTAGGGTTTGAAGGATCTAATTCAATTACTTGTGCTACTTTTGTTGAATTTATTCATACCGCTTCT
TTGTTACACGATGATGTCGTAGATGAGTCTGATATGCGTCGTGGGCGTGCTACGGCGAATGCTGAATTTGGCAATGCGGC
GAGTGTGTTGGTAGGCGATTTTATTTATACGCGTGCATTTCAATTAGTTGCACAGTTAGAATCATTGAAAATTTTAAGTA
TTATGGCTGATGCGACTAATGTTTTAGCGGAAGGCGAAGTTCAGCAGTTAATGAATGTTAATGATCCCGAAACGAGCGAA
GCTAATTATATGCGTGTAATTTATAGTAAAACTGCGCGTTTGTTTGAAGTGGCTGGTCAGGCTGCGGCAATTGTCGCCGG
TGGAACAGAAGCTCAAGAAAAGGCGTTACAAGATTATGGGCGTTATCTTGGCACTGCATTTCAGCTCGTGGATGATGTAT
TGGATTATAGTGCAAATGCGCAAGCTCTAGGCAAAAATGTAGGCGATGATTTAGCTGAAGGAAAACCAACCCTTCCTCTG
CTACACGCTATGCGTCATGGAAATGCTCAACAGGCAGCTTTAATTCGCGAAGCGATTGAGCAAGGTGGTAAACGTGAAGC
AATTGATGAAGTATTAGCTATTATGACTGAGCATAAGTCTTTAGATTACGCAATGAATCGTGCAAAAGAAGAGGCTCAAA
AAGCAGTGGATGCAATCGAAATTTTGCCAGAAAGTGAATATAAACAAGCGTTGATTTCATTGGCATATTTGTCTGTAGAT
AGAAATTATTAG

Upstream 100 bases:

>100_bases
TCCCTTCATTTGCGGATTGATTTCAGAAAGTCTGAAAATTCGGGTAAAATGCACCGCACTTTTGACTGATAAATGATGAA
AAATGAAGAAACAAGATCTT

Downstream 100 bases:

>100_bases
TCCATAATTTAGCTTATATACTTGATACCCATTAATTTTAATTAAACTTTATGAATACAGAACTTCAACCTAAACTAGAA
AAAAGTGCGGTCAATTTTCA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVLAARSLGFEGSNSITCATFVEFIHTAS
LLHDDVVDESDMRRGRATANAEFGNAASVLVGDFIYTRAFQLVAQLESLKILSIMADATNVLAEGEVQQLMNVNDPETSE
ANYMRVIYSKTARLFEVAGQAAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANAQALGKNVGDDLAEGKPTLPL
LHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQKAVDAIEILPESEYKQALISLAYLSVD
RNY

Sequences:

>Translated_323_residues
MSIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVLAARSLGFEGSNSITCATFVEFIHTAS
LLHDDVVDESDMRRGRATANAEFGNAASVLVGDFIYTRAFQLVAQLESLKILSIMADATNVLAEGEVQQLMNVNDPETSE
ANYMRVIYSKTARLFEVAGQAAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANAQALGKNVGDDLAEGKPTLPL
LHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQKAVDAIEILPESEYKQALISLAYLSVD
RNY
>Mature_322_residues
SIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVLAARSLGFEGSNSITCATFVEFIHTASL
LHDDVVDESDMRRGRATANAEFGNAASVLVGDFIYTRAFQLVAQLESLKILSIMADATNVLAEGEVQQLMNVNDPETSEA
NYMRVIYSKTARLFEVAGQAAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANAQALGKNVGDDLAEGKPTLPLL
HAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQKAVDAIEILPESEYKQALISLAYLSVDR
NY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI4758430, Length=269, Percent_Identity=24.5353159851301, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI83700220, Length=269, Percent_Identity=24.5353159851301, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=64.9068322981366, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1786623, Length=228, Percent_Identity=29.8245614035088, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=333, Percent_Identity=33.9339339339339, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319475, Length=287, Percent_Identity=33.4494773519164, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325188, Length=259, Percent_Identity=23.5521235521236, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24651612, Length=302, Percent_Identity=31.1258278145695, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI281365769, Length=245, Percent_Identity=28.1632653061224, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24660002, Length=245, Percent_Identity=28.1632653061224, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35138; Mature: 35007

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVLAARSLG
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHCC
FEGSNSITCATFVEFIHTASLLHDDVVDESDMRRGRATANAEFGNAASVLVGDFIYTRAF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
QLVAQLESLKILSIMADATNVLAEGEVQQLMNVNDPETSEANYMRVIYSKTARLFEVAGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANAQALGKNVGDDLAEGKPTLPL
HHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHCCCCCHHH
LHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQKAVDAIE
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPESEYKQALISLAYLSVDRNY
HCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVLAARSLG
CHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHCC
FEGSNSITCATFVEFIHTASLLHDDVVDESDMRRGRATANAEFGNAASVLVGDFIYTRAF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
QLVAQLESLKILSIMADATNVLAEGEVQQLMNVNDPETSEANYMRVIYSKTARLFEVAGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANAQALGKNVGDDLAEGKPTLPL
HHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHCCCCCHHH
LHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQKAVDAIE
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPESEYKQALISLAYLSVDRNY
HCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]