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The map label for this gene is def

Identifier: 148827778

GI number: 148827778

Start: 1131792

End: 1132301

Strand: Reverse

Name: def

Synonym: CGSHiGG_06235

Alternate gene names: 148827778

Gene position: 1132301-1131792 (Counterclockwise)

Preceding gene: 148827779

Following gene: 148827777

Centisome position: 60.0

GC content: 36.47

Gene sequence:

>510_bases
ATGACCGCACTTAATGTATTAATTTATCCTGATGATCACTTAAAGGTTGTCTGTGAGCCTGTTACTGAAGTTAATGATGC
AATTCGTAAAATTGTTGATGATATGTTTGATACGATGTATCAAGAGAAAGGTATTGGTTTAGCTGCACCTCAGGTGGATA
TTTTGCAACGTATTATTACTATTGATGTTGAAGGCGATAAACAGAATCAGTTTGTTTTGATTAACCCTGAAATTTTGGCT
TCTGAAGGCGAAACGGGAATTGAAGAGGGATGTTTATCCATTCCAGGGTTCCGTGCGTTGGTTCCTCGTAAAGAAAAAGT
GACAGTGAGAGCGTTAGATCGTGATGGTAAAGAATTTACTTTAGATGCGGATGGTTTGCTTGCGATCTGTATTCAACACG
AGATCGATCATCTTAATGGAATTTTATTTGTTGATTATCTTTCTCCACTTAAACGCCAACGTATTAAAGAAAAGTTGATT
AAATACAAGAAGCAGATTGCGAAAAGCTAA

Upstream 100 bases:

>100_bases
GCTTAACCTCAGCCCCGCCCCATTTGGGGCTTTTCTACACATTCTTTCTTAGCTGTGCTATAATCCCACTAAAATTCTAC
CCTTAAAAACAAGAGAAATT

Downstream 100 bases:

>100_bases
AACGTAGGGTGGGCTTTAGCCCATCAATCATTTCAAAAAACGGTGGGCTTAAGCCCACCCTACTTCCTATCAATTACCAT
AACGTAGAACATTATGAAAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MTALNVLIYPDDHLKVVCEPVTEVNDAIRKIVDDMFDTMYQEKGIGLAAPQVDILQRIITIDVEGDKQNQFVLINPEILA
SEGETGIEEGCLSIPGFRALVPRKEKVTVRALDRDGKEFTLDADGLLAICIQHEIDHLNGILFVDYLSPLKRQRIKEKLI
KYKKQIAKS

Sequences:

>Translated_169_residues
MTALNVLIYPDDHLKVVCEPVTEVNDAIRKIVDDMFDTMYQEKGIGLAAPQVDILQRIITIDVEGDKQNQFVLINPEILA
SEGETGIEEGCLSIPGFRALVPRKEKVTVRALDRDGKEFTLDADGLLAICIQHEIDHLNGILFVDYLSPLKRQRIKEKLI
KYKKQIAKS
>Mature_168_residues
TALNVLIYPDDHLKVVCEPVTEVNDAIRKIVDDMFDTMYQEKGIGLAAPQVDILQRIITIDVEGDKQNQFVLINPEILAS
EGETGIEEGCLSIPGFRALVPRKEKVTVRALDRDGKEFTLDADGLLAICIQHEIDHLNGILFVDYLSPLKRQRIKEKLIK
YKKQIAKS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=27.3885350318471, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=65.6804733727811, Blast_Score=223, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24645728, Length=137, Percent_Identity=34.3065693430657, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24645726, Length=166, Percent_Identity=27.710843373494, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_HAEI8 (Q4QMV6)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248301.1
- HSSP:   P0A6K3
- ProteinModelPortal:   Q4QMV6
- SMR:   Q4QMV6
- STRING:   Q4QMV6
- GeneID:   3430033
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0725
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- PhylomeDB:   Q4QMV6
- ProtClustDB:   PRK00150
- BioCyc:   HINF281310:NTHI0725-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19058; Mature: 18927

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALNVLIYPDDHLKVVCEPVTEVNDAIRKIVDDMFDTMYQEKGIGLAAPQVDILQRIIT
CCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHE
IDVEGDKQNQFVLINPEILASEGETGIEEGCLSIPGFRALVPRKEKVTVRALDRDGKEFT
EEECCCCCCCEEEECCCEEECCCCCCHHHCCCCCCCCHHHCCCCCCEEEEEECCCCCEEE
LDADGLLAICIQHEIDHLNGILFVDYLSPLKRQRIKEKLIKYKKQIAKS
ECCCCCEEHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TALNVLIYPDDHLKVVCEPVTEVNDAIRKIVDDMFDTMYQEKGIGLAAPQVDILQRIIT
CEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHE
IDVEGDKQNQFVLINPEILASEGETGIEEGCLSIPGFRALVPRKEKVTVRALDRDGKEFT
EEECCCCCCCEEEECCCEEECCCCCCHHHCCCCCCCCHHHCCCCCCEEEEEECCCCCEEE
LDADGLLAICIQHEIDHLNGILFVDYLSPLKRQRIKEKLIKYKKQIAKS
ECCCCCEEHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA