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The map label for this gene is fmt [H]

Identifier: 148827777

GI number: 148827777

Start: 1130742

End: 1131698

Strand: Reverse

Name: fmt [H]

Synonym: CGSHiGG_06230

Alternate gene names: 148827777

Gene position: 1131698-1130742 (Counterclockwise)

Preceding gene: 148827778

Following gene: 148827776

Centisome position: 59.97

GC content: 38.87

Gene sequence:

>957_bases
ATGAAATCACTTAATATTATTTTTGCTGGTACGCCAGATTTTGCTGCACAGCATTTACAAGCCATTCTAAATTCTCAACA
TAATGTGATCGCTGTTTATACTCAACCTGATAAACCAGCTGGTCGCGGTAAGAAATTGCAAGCAAGTCCTGTAAAGCAAC
TTGCTGAGCAAAATAACATTCCCGTTTATCAACCTAAATCCTTACGTAAAGAAGAGGCTCAGTCCGAATTAAAAGCGTTA
AATGCAGATGTAATGGTTGTTGTGGCTTATGGATTAATTTTACCGAAAGCTGTATTAGATGCCCCTCGTTTGGGTTGTTT
GAATGTGCATGGTTCTATTCTTCCACGTTGGCGAGGTGCAGCACCAATTCAGCGTTCAATTTGGGCTGGCGATGTACAAA
CGGGTGTAACCATTATGCAAATGGATGAAAGTTTAGATACAGGCGATATGTTACATAAAGTCTATTGTGATATTTTACCG
ACTGAAACTTCAACGAGTCTTTATAACAAACTGGCAGAGCTTGCTACATCAGCATTAATCGATGTTTTAGATAATCTTGA
AAACAGTAAATTTATAGCGGAAAAACAAGATGGCAGCCAAAGTAATTATGCAGAAAAACTTTCCAAAGAAGAGGCTCAAT
TAGATTGGTCACTTCCTGCAATGCAACTTGAGCGTAATATCCGCGCTTTTAATCCTTGGCCAATTGCCTATTTTTCAACA
GAAGACAAGGATGGCAATGCACAAACTTTAAAAGTGTATCAAGCGGAAGTGTTGCCTCATCAAGATAAACCAGCGGGAAC
TATTTTAAGTGCGGATAAAAATGGCATTCAAATTGCGACTGTCGATGGCGTCTTAAACTTATTGCAATTGCAACCTGCAG
GTAAAAAGCCTATGTCTGCACAAGATTTATTAAATGGCCGTGCAGAATGGTTTACTATTGGTAAGGTGCTTGCATAA

Upstream 100 bases:

>100_bases
AAGCTAAAACGTAGGGTGGGCTTTAGCCCATCAATCATTTCAAAAAACGGTGGGCTTAAGCCCACCCTACTTCCTATCAA
TTACCATAACGTAGAACATT

Downstream 100 bases:

>100_bases
TGAAAAAATTCTCTTCTAAAACTATCAAAGCAAAAAATTCAGTAAAAATGACCGCACTTTCGACCAGGGCTATTGCAGCA
AACTTAATTTTGCAAGTATT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNIPVYQPKSLRKEEAQSELKAL
NADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGAAPIQRSIWAGDVQTGVTIMQMDESLDTGDMLHKVYCDILP
TETSTSLYNKLAELATSALIDVLDNLENSKFIAEKQDGSQSNYAEKLSKEEAQLDWSLPAMQLERNIRAFNPWPIAYFST
EDKDGNAQTLKVYQAEVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQPAGKKPMSAQDLLNGRAEWFTIGKVLA

Sequences:

>Translated_318_residues
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNIPVYQPKSLRKEEAQSELKAL
NADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGAAPIQRSIWAGDVQTGVTIMQMDESLDTGDMLHKVYCDILP
TETSTSLYNKLAELATSALIDVLDNLENSKFIAEKQDGSQSNYAEKLSKEEAQLDWSLPAMQLERNIRAFNPWPIAYFST
EDKDGNAQTLKVYQAEVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQPAGKKPMSAQDLLNGRAEWFTIGKVLA
>Mature_318_residues
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNIPVYQPKSLRKEEAQSELKAL
NADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGAAPIQRSIWAGDVQTGVTIMQMDESLDTGDMLHKVYCDILP
TETSTSLYNKLAELATSALIDVLDNLENSKFIAEKQDGSQSNYAEKLSKEEAQLDWSLPAMQLERNIRAFNPWPIAYFST
EDKDGNAQTLKVYQAEVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQPAGKKPMSAQDLLNGRAEWFTIGKVLA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=220, Percent_Identity=33.1818181818182, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI21614513, Length=240, Percent_Identity=27.9166666666667, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI238814322, Length=303, Percent_Identity=27.0627062706271, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1789683, Length=317, Percent_Identity=64.0378548895899, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1788589, Length=301, Percent_Identity=26.9102990033223, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI133930964, Length=324, Percent_Identity=24.6913580246914, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319458, Length=264, Percent_Identity=25.7575757575758, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45550868, Length=319, Percent_Identity=31.3479623824451, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28571984, Length=242, Percent_Identity=33.8842975206612, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585660, Length=328, Percent_Identity=24.390243902439, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34866; Mature: 34866

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
PVYQPKSLRKEEAQSELKALNADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRSIWAGDVQTGVTIMQMDESLDTGDMLHKVYCDILPTETSTSLYNKLAELATSALI
CCHHHHHCCCCHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DVLDNLENSKFIAEKQDGSQSNYAEKLSKEEAQLDWSLPAMQLERNIRAFNPWPIAYFST
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEC
EDKDGNAQTLKVYQAEVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQPAGKKPMSA
CCCCCCCEEEEEEEHHHCCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHCCCCCCCCCCH
QDLLNGRAEWFTIGKVLA
HHHHCCCHHEEECCHHCC
>Mature Secondary Structure
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
PVYQPKSLRKEEAQSELKALNADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRSIWAGDVQTGVTIMQMDESLDTGDMLHKVYCDILPTETSTSLYNKLAELATSALI
CCHHHHHCCCCHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DVLDNLENSKFIAEKQDGSQSNYAEKLSKEEAQLDWSLPAMQLERNIRAFNPWPIAYFST
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEC
EDKDGNAQTLKVYQAEVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQPAGKKPMSA
CCCCCCCEEEEEEEHHHCCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHCCCCCCCCCCH
QDLLNGRAEWFTIGKVLA
HHHHCCCHHEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA