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The map label for this gene is yfiF [C]

Identifier: 148827623

GI number: 148827623

Start: 969836

End: 970951

Strand: Reverse

Name: yfiF [C]

Synonym: CGSHiGG_05330

Alternate gene names: 148827623

Gene position: 970951-969836 (Counterclockwise)

Preceding gene: 148827625

Following gene: 148827621

Centisome position: 51.45

GC content: 38.98

Gene sequence:

>1116_bases
ATGCTCACAAATTTTAGCATTTTTTGGCTAAAAAACAGCCATTTTTACTCAGGGAAACCCTATGAACGACAAACGCAAAC
CCTCTTTTCAATCCGCAGGCAAAACCTTTCAAGAACGCTCAGTGGGGAAAAGTACAGAGAAAAACCAACTCAAAATCGAC
CGCACTTTAATGATAAATTTAATGGAAATAGAAACGAAAAATCTCGTTTTTCTCGTGATAAACAAGAAGTGAAAGAAACT
AGAATTACGCAACTTTCTTTATCTCGTGCGCCATCAAATAAAAATGCAGAACAACCTAAGGTTCAAGTGACAATAAAAAG
TACGGGTACGGTTTATAAAACAAAAGAAAAGAAAACTGGTGCGCTTTCTCCTCGAGCACCTGAAAAAATTAAGAAAAACC
GTGCGGAAGAAATGAAAGTTTACGGTGAAAATGCCTGTTTAGCTTTATTTGCAGAACGTCCTGAAAGTATTGTTCGATTG
TGGGCAACCGTACAAATGTCACACAAAATTGGCGAGGTATTGAGTTATTTAGCCGAAAACAAGAAGGCTTATCACGTTGT
AGATAGTGAAGAATTAGCTCGAGTGAGCGGAACAGAACATCACGGTGGCATTTGCTTATTAGTGAAGAAACCGCGTGTTT
TCACGTTGCAAGGGTATTTGGATATTCCTCATAAAGAAGATTGTTTAGTGCTATTAGACAACGTGAATAATGCACAAAAT
ATTGGTGGCGTATTGCGTACTTGTGCTTATTTTGGCGTGAAAAATATTGTCGCCGATAATGTGGAAAATCTGTATTCAGC
AGCCTCAATGCGTGTAGCAGAAGGTGGCGCAGAATATATTCGTGTGTTAGAAACGGATGATATTGATTCGGCTTTGATGC
ACTTGCGTAAATCAGGCTATCAAGTTATTCACGTATCGCACAATAAACAAGGCGACCCATTGGATAAAGTGCGGTTAAAA
AATAAAGTGGTTTTTGTGCTAAGTGAAAGTTCTACGGAAAGTTTAGCGACGCCAGAAGATACACAAGTGCGTTTAACGCT
CGCAAGCCCAATTAAATCAGGCTTAAACATTGCAGTGAATGCGGGTGTGTTGTTAGCGAAGTGGTATTTTAGATAG

Upstream 100 bases:

>100_bases
TTTATTGATTAACATAATCCATCTCGTGATTAAAGACTAGTGCGTTAGTTTACAATATTTCACGTGCTTATTAATAGAAA
AAAGGCTTTTTTTCGTTATA

Downstream 100 bases:

>100_bases
TGTATGATTAAAGGGGAAAATGATTTCCCCTTTAAAATTTCAAATAAAAGTCTTTAGTCAATTTAATAAACGCTTCCGTG
TATTGATTATCCGCATCATA

Product: ATP-dependent RNA helicase SrmB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLTNFSIFWLKNSHFYSGKPYERQTQTLFSIRRQNLSRTLSGEKYREKPTQNRPHFNDKFNGNRNEKSRFSRDKQEVKET
RITQLSLSRAPSNKNAEQPKVQVTIKSTGTVYKTKEKKTGALSPRAPEKIKKNRAEEMKVYGENACLALFAERPESIVRL
WATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSGTEHHGGICLLVKKPRVFTLQGYLDIPHKEDCLVLLDNVNNAQN
IGGVLRTCAYFGVKNIVADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMHLRKSGYQVIHVSHNKQGDPLDKVRLK
NKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVNAGVLLAKWYFR

Sequences:

>Translated_371_residues
MLTNFSIFWLKNSHFYSGKPYERQTQTLFSIRRQNLSRTLSGEKYREKPTQNRPHFNDKFNGNRNEKSRFSRDKQEVKET
RITQLSLSRAPSNKNAEQPKVQVTIKSTGTVYKTKEKKTGALSPRAPEKIKKNRAEEMKVYGENACLALFAERPESIVRL
WATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSGTEHHGGICLLVKKPRVFTLQGYLDIPHKEDCLVLLDNVNNAQN
IGGVLRTCAYFGVKNIVADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMHLRKSGYQVIHVSHNKQGDPLDKVRLK
NKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVNAGVLLAKWYFR
>Mature_371_residues
MLTNFSIFWLKNSHFYSGKPYERQTQTLFSIRRQNLSRTLSGEKYREKPTQNRPHFNDKFNGNRNEKSRFSRDKQEVKET
RITQLSLSRAPSNKNAEQPKVQVTIKSTGTVYKTKEKKTGALSPRAPEKIKKNRAEEMKVYGENACLALFAERPESIVRL
WATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSGTEHHGGICLLVKKPRVFTLQGYLDIPHKEDCLVLLDNVNNAQN
IGGVLRTCAYFGVKNIVADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMHLRKSGYQVIHVSHNKQGDPLDKVRLK
NKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVNAGVLLAKWYFR

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=316, Percent_Identity=38.6075949367089, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 41971; Mature: 41971

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNFSIFWLKNSHFYSGKPYERQTQTLFSIRRQNLSRTLSGEKYREKPTQNRPHFNDKF
CCCCEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
NGNRNEKSRFSRDKQEVKETRITQLSLSRAPSNKNAEQPKVQVTIKSTGTVYKTKEKKTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCC
ALSPRAPEKIKKNRAEEMKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAEN
CCCCCCHHHHHHHHHHHHHEECCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KKAYHVVDSEELARVSGTEHHGGICLLVKKPRVFTLQGYLDIPHKEDCLVLLDNVNNAQN
CCEEEEECHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCCEEEEECCCCHHH
IGGVLRTCAYFGVKNIVADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMHLRKSGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCC
QVIHVSHNKQGDPLDKVRLKNKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVN
EEEEEECCCCCCCHHHHEECCEEEEEEECCCCCCCCCCCCCEEEEEEECCHHCCCEEEEE
AGVLLAKWYFR
CCEEEEEEECC
>Mature Secondary Structure
MLTNFSIFWLKNSHFYSGKPYERQTQTLFSIRRQNLSRTLSGEKYREKPTQNRPHFNDKF
CCCCEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
NGNRNEKSRFSRDKQEVKETRITQLSLSRAPSNKNAEQPKVQVTIKSTGTVYKTKEKKTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCC
ALSPRAPEKIKKNRAEEMKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAEN
CCCCCCHHHHHHHHHHHHHEECCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KKAYHVVDSEELARVSGTEHHGGICLLVKKPRVFTLQGYLDIPHKEDCLVLLDNVNNAQN
CCEEEEECHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCCEEEEECCCCHHH
IGGVLRTCAYFGVKNIVADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMHLRKSGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCC
QVIHVSHNKQGDPLDKVRLKNKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVN
EEEEEECCCCCCCHHHHEECCEEEEEEECCCCCCCCCCCCCEEEEEEECCHHCCCEEEEE
AGVLLAKWYFR
CCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]