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The map label for this gene is hsdM [H]

Identifier: 148827354

GI number: 148827354

Start: 688707

End: 690251

Strand: Direct

Name: hsdM [H]

Synonym: CGSHiGG_03695

Alternate gene names: 148827354

Gene position: 688707-690251 (Clockwise)

Preceding gene: 148827353

Following gene: 148827355

Centisome position: 36.49

GC content: 40.32

Gene sequence:

>1545_bases
ATGGCTGCTGCAATTCAACAACGTGCCGAACTTCAACGTCGTATTTGGCAAATTGCTAATGATGTGCGAGGTTCTGTCGA
TGGCTGGGATTTCAAACAATATGTGCTTGGCACACTTTTTTACCGTTTTATTAGCGAAAATTTTGCCAATTACATTGAAG
CGGGCGATGAAAGCGTAAATTATGCCCAATTACCTGATGAAATCATTACCCCAGACATTAAAACAGATGCCATTAAAACG
AAAGGCTACTTTATTTACCCAAGCCAATTATTTAAGAATCTCGCAGCTAATGCTGGCAGCAATCCTAATTTGAATACTGA
TTTAAAACAGATTTTTACTGATATTGAAAACTCAGCGACGGGCTTTCCGTCTGAACAAGATATTAAAGGCTTATTTGCCG
ATTTTGACACCACCAGCAATCGCTTAGGCAATACCGTAAAAGATAAAAACGACCGCTTAACGGCTGTTTTGAAAGGCGTG
GCTGAACTTGATTTTGGCAAATTTGAAGATAACCACATTGATTTATTTGGCGATGCATACGAATATCTTATTTCTAACTA
TGCTGCCAATGCAGGCAAATCTGGTGGCGAATTTTTTACCCCACAAAGTGTTTCCAAACTTATTGCTCAAATTGCAATGC
ACGGGCAAACTTCGGTCAATAAAATTTATGACCCTGCAGCAGGTTCTGGCTCACTTTTGCTTCAAGCCAAAAAACAATTT
GATGAACATATTATTGAAGAAGGCTTTTTCGGGCAGGAAATTAACCATACCACATACAACCTTGCCCGTATGAATATGTT
TTTGCATAACATCAACTACGACAAGTTTGATATTGCTTTAGGCAACACCTTAATGGAACCACAATTTGGCGATGATAAAC
CTTTCGATGCCATTGTTTCTAACCCGCCTTACTCCGTGAAATGGGCTGGCTCCGACGATCCAACATTGATTAATGATGAA
CGATTTGCCCCCGCAGGCGTGCTTGCACCAAAATCCAAAGCGGACTTTGCCTTTATTTTACATGCGTTAAGTTATCTTTC
AGCAAAAGGCCGCGCGGCGATTGTTTCCTTCCCTGGTATTTTTTATCGTGGCGGTGCCGAGCAAAAAATTCGTCAATATT
TGGTGGATAATAACTATGTGGACGCGGTGATTGCGCTTGCGCCAAATCTCTTTTTTGGCACCAGTATTGCCGTGAATATT
TTGGTGCTTTCCAAACACAAACCCAATACCCAAACCCAATTTATCGATGCCAGCGGTTTATTTAAATCTGCCACTAATAA
CAACATTTTAGAAGAGGAACATATTGAGCAAATTCTCAAACTGTTTGCCGATAAACAAGATGTGCCGCATTTGGCAAAAT
CCATATCCTTTGAAGAAATCGCCCAAAATGACTACAACCTTGCAGTAAGTTCGTATGTGGAACAAAAAGACACTCGTGAA
GTGATTAATATTGATGAACTCAATGCTCAAATTCGTAAAACTGTTGCCAATATCGACCGCTTGCGTGCGGAGATTGACAA
GATTGTGGCAGAAATTGAGGGATAA

Upstream 100 bases:

>100_bases
GTAAGATAAACGCATAGAAATTCACGCAGGATAGACTAATTTCCTGTATAATTTTTCACATATCTATTCAACTCATTACA
CAAAATATTCTGAGGATTTT

Downstream 100 bases:

>100_bases
AATGTAGGGTGGGCTTCAGCCCACCAATAAGATTGAAAAATAACAGCAAACCGTAGGGTGGGCTTCAGCCCACCAATTTA
CGATAACAGGTGGTGGGCTA

Product: putative type I restriction-modification system methyltransferase subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MAAAIQQRAELQRRIWQIANDVRGSVDGWDFKQYVLGTLFYRFISENFANYIEAGDESVNYAQLPDEIITPDIKTDAIKT
KGYFIYPSQLFKNLAANAGSNPNLNTDLKQIFTDIENSATGFPSEQDIKGLFADFDTTSNRLGNTVKDKNDRLTAVLKGV
AELDFGKFEDNHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIAQIAMHGQTSVNKIYDPAAGSGSLLLQAKKQF
DEHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIALGNTLMEPQFGDDKPFDAIVSNPPYSVKWAGSDDPTLINDE
RFAPAGVLAPKSKADFAFILHALSYLSAKGRAAIVSFPGIFYRGGAEQKIRQYLVDNNYVDAVIALAPNLFFGTSIAVNI
LVLSKHKPNTQTQFIDASGLFKSATNNNILEEEHIEQILKLFADKQDVPHLAKSISFEEIAQNDYNLAVSSYVEQKDTRE
VINIDELNAQIRKTVANIDRLRAEIDKIVAEIEG

Sequences:

>Translated_514_residues
MAAAIQQRAELQRRIWQIANDVRGSVDGWDFKQYVLGTLFYRFISENFANYIEAGDESVNYAQLPDEIITPDIKTDAIKT
KGYFIYPSQLFKNLAANAGSNPNLNTDLKQIFTDIENSATGFPSEQDIKGLFADFDTTSNRLGNTVKDKNDRLTAVLKGV
AELDFGKFEDNHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIAQIAMHGQTSVNKIYDPAAGSGSLLLQAKKQF
DEHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIALGNTLMEPQFGDDKPFDAIVSNPPYSVKWAGSDDPTLINDE
RFAPAGVLAPKSKADFAFILHALSYLSAKGRAAIVSFPGIFYRGGAEQKIRQYLVDNNYVDAVIALAPNLFFGTSIAVNI
LVLSKHKPNTQTQFIDASGLFKSATNNNILEEEHIEQILKLFADKQDVPHLAKSISFEEIAQNDYNLAVSSYVEQKDTRE
VINIDELNAQIRKTVANIDRLRAEIDKIVAEIEG
>Mature_513_residues
AAAIQQRAELQRRIWQIANDVRGSVDGWDFKQYVLGTLFYRFISENFANYIEAGDESVNYAQLPDEIITPDIKTDAIKTK
GYFIYPSQLFKNLAANAGSNPNLNTDLKQIFTDIENSATGFPSEQDIKGLFADFDTTSNRLGNTVKDKNDRLTAVLKGVA
ELDFGKFEDNHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIAQIAMHGQTSVNKIYDPAAGSGSLLLQAKKQFD
EHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIALGNTLMEPQFGDDKPFDAIVSNPPYSVKWAGSDDPTLINDER
FAPAGVLAPKSKADFAFILHALSYLSAKGRAAIVSFPGIFYRGGAEQKIRQYLVDNNYVDAVIALAPNLFFGTSIAVNIL
VLSKHKPNTQTQFIDASGLFKSATNNNILEEEHIEQILKLFADKQDVPHLAKSISFEEIAQNDYNLAVSSYVEQKDTREV
INIDELNAQIRKTVANIDRLRAEIDKIVAEIEG

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=375, Percent_Identity=25.8666666666667, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 57259; Mature: 57128

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAAIQQRAELQRRIWQIANDVRGSVDGWDFKQYVLGTLFYRFISENFANYIEAGDESVN
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YAQLPDEIITPDIKTDAIKTKGYFIYPSQLFKNLAANAGSNPNLNTDLKQIFTDIENSAT
HHHCCHHHCCCCCCCCCEECCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
GFPSEQDIKGLFADFDTTSNRLGNTVKDKNDRLTAVLKGVAELDFGKFEDNHIDLFGDAY
CCCCHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHH
EYLISNYAANAGKSGGEFFTPQSVSKLIAQIAMHGQTSVNKIYDPAAGSGSLLLQAKKQF
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHH
DEHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIALGNTLMEPQFGDDKPFDAIVS
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHC
NPPYSVKWAGSDDPTLINDERFAPAGVLAPKSKADFAFILHALSYLSAKGRAAIVSFPGI
CCCCEEEECCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCE
FYRGGAEQKIRQYLVDNNYVDAVIALAPNLFFGTSIAVNILVLSKHKPNTQTQFIDASGL
EECCCHHHHHHHHHHCCCHHHHHHHHHCCHHCCCHHEEEEEEEECCCCCCCCEEEECCCC
FKSATNNNILEEEHIEQILKLFADKQDVPHLAKSISFEEIAQNDYNLAVSSYVEQKDTRE
HHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHH
VINIDELNAQIRKTVANIDRLRAEIDKIVAEIEG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]