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The map label for this gene is rnhA

Identifier: 148827279

GI number: 148827279

Start: 610072

End: 610536

Strand: Direct

Name: rnhA

Synonym: CGSHiGG_03270

Alternate gene names: 148827279

Gene position: 610072-610536 (Clockwise)

Preceding gene: 148827276

Following gene: 148827280

Centisome position: 32.33

GC content: 35.91

Gene sequence:

>465_bases
ATGCAAAAACAGATTGAAATTTTTACTGATGGATCTTGCTTAGGTAATCCAGGGGCGGGCGGAATTGGTGCCGTATTGCG
TTATAAACAGCATGAAAAAATGCTCTCCAAAGGCTATTTCAAAACCACCAATAATCGAATGGAATTACGCGCTGTCATTG
AAGCATTAAATACATTAAAAGAACCTTGCTTGATCACGCTTTATAGTGATAGCCAATATATGAAAAACGGCATAACAAAA
TGGATCTTTAACTGGAAAAAAAATAATTGGAAAGCAAGTTCTGGAAAGCCTGTAAAAAACCAAGATTTATGGATAGCCTT
AGATGAATCCATCCAACGTCATAAAATTAATTGGCAATGGGTAAAAGGTCATGCAGGACACAGAGAAAATGAAATTTGCG
ATGAATTAGCAAAAAAAGGAGCAGAAAATCCGACATTGGAAGATATGGGGTACATAGAAGAATAA

Upstream 100 bases:

>100_bases
TTTCAGTATCTAATACAATTTGGCGATTGGGATTAATCATTTGTTCAACCTCTCTCTTTCCATTAAAATACTTGCCATTC
TACACAACAACCTTTTTGTT

Downstream 100 bases:

>100_bases
TACAACTGATATAACATCATATTTTTCGATACCTAAAAATATTTAATACTTAAACCTAAAAAAGAACAAAAAACAATCAA
ATTCCTTTAAAAAAATGTGA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MQKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKMLSKGYFKTTNNRMELRAVIEALNTLKEPCLITLYSDSQYMKNGITK
WIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQWVKGHAGHRENEICDELAKKGAENPTLEDMGYIEE

Sequences:

>Translated_154_residues
MQKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKMLSKGYFKTTNNRMELRAVIEALNTLKEPCLITLYSDSQYMKNGITK
WIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQWVKGHAGHRENEICDELAKKGAENPTLEDMGYIEE
>Mature_154_residues
MQKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKMLSKGYFKTTNNRMELRAVIEALNTLKEPCLITLYSDSQYMKNGITK
WIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQWVKGHAGHRENEICDELAKKGAENPTLEDMGYIEE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=148, Percent_Identity=36.4864864864865, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=64.9006622516556, Blast_Score=212, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=35.4609929078014, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17505482, Length=131, Percent_Identity=35.8778625954198, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17137476, Length=142, Percent_Identity=35.9154929577465, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=35.4166666666667, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_HAEI8 (Q4QP49)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_247858.1
- ProteinModelPortal:   Q4QP49
- SMR:   Q4QP49
- STRING:   Q4QP49
- GeneID:   3429564
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0224
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q4QP49
- ProtClustDB:   PRK00203
- BioCyc:   HINF281310:NTHI0224-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17701; Mature: 17701

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKMLSKGYFKTTNNRMELRAVIEALNTLK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCC
EPCLITLYSDSQYMKNGITKWIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQW
CCEEEEEECCCHHHHCCHHHHHHCCCCCCEECCCCCCCCCCCEEEEECCCHHHHCCCEEE
VKGHAGHRENEICDELAKKGAENPTLEDMGYIEE
EECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKMLSKGYFKTTNNRMELRAVIEALNTLK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCC
EPCLITLYSDSQYMKNGITKWIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQW
CCEEEEEECCCHHHHCCHHHHHHCCCCCCEECCCCCCCCCCCEEEEECCCHHHHCCCEEE
VKGHAGHRENEICDELAKKGAENPTLEDMGYIEE
EECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA