Click here to switch to the map view.

The map label for this gene is cysS [H]

Identifier: 148827235

GI number: 148827235

Start: 542029

End: 543024

Strand: Reverse

Name: cysS [H]

Synonym: CGSHiGG_02970

Alternate gene names: 148827235

Gene position: 543024-542029 (Counterclockwise)

Preceding gene: 148827236

Following gene: 148827234

Centisome position: 28.77

GC content: 39.26

Gene sequence:

>996_bases
ATGAAAATTGCAATCTATAGCACTAAAAGTTATGACAGAAAATATATTGAGCTCATTAACGCGAAATATAATTTCGATTT
AGAATTTTTTGATTTTATGCTGAACGAGAGCACTGTGCGTTTGGCAGAACATTGCGAAGTAGTATGCATTTTCGTGAATG
ATAATGGTAGCCGAAAAGTATTAGAAAAATTAGCCGCACTTGGTGTGAAAATTGTGGCTTTGCGCTGTGCCGGTTTCAAT
AATGTTGATTTAAAAGCAGCTCAAGAATTAGGCATCCAAGTTGTCCGCGTTCCAGCTTATTCGCCTGAAGCGGTGGCGGA
ACATACCATCGGTTTGATGATGACACTGAATCGTCGAATTCATCGTGCTTATCAGCGTACCCGAGAAGCGAATTTCTCTC
TTGAGGGGTTAATTGGTTTCAATATGTACGGACGCACAGTCGGCGTAATCGGAACAGGAAAAATCGGGATCGCAGTGATG
CGAATTTTGAAAGGTTTTGGCATGAATATCTTGGCGTACGATCCTTTTAAAAATCCAGTGGTGGAAGAACTGGGGGGACA
ATATGTTGAATTAGATGAGCTTTATGCTAAATCTCATGTGATTACGCTTCATTGCCCAGCAACGCCAGAAAACTATCATT
TATTAAATCGCAAGGCTTTTGCAAAAATGAAAGATGGCGTGATGATCGTTAATACGAGTCGCGGTTCTTTGATTGATACT
CAAGCTGCAATTGATGCGTTAAAACAGCGTAAAATCGGTGCGTTAGGTATGGATGTTTACGAGAATGAACGTGATTTATT
CTTTGAAGATAAGTCTAACGAAGTGATTCAAGATGATATTTTCCGCCGTTTATCTTCTTGCCATAATGTATTGCTAACAG
GTCACCAAGCGTTTTTAACGGAAGAGGCACTAACCAATATTGCGGATGTGACTTTATCAAACATTTATAAATTGAAATCG
GGTAAAGTGTGTGAAAATATTGTTTTGCCGTCTTAG

Upstream 100 bases:

>100_bases
TAATACCTTGTTGCGTTTTTCAGTCGGTATTGAAGATGTAGAAGATATTAAAGCGGACTTATTACAAGCTTTTGCAAACT
TAAAATAATTGGAGCAAACC

Downstream 100 bases:

>100_bases
CTTTTGGCAATTAAAACGATTAGTTTACATTTTTTAAAACGGTGTTATTCTGCACCGCACTTGTAAGTTTAAAAAAGCCC
TTATATAGGGATTCCATTCA

Product: cysteinyl-tRNA synthetase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKVLEKLAALGVKIVALRCAGFN
NVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRIHRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVM
RILKGFGMNILAYDPFKNPVVEELGGQYVELDELYAKSHVITLHCPATPENYHLLNRKAFAKMKDGVMIVNTSRGSLIDT
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLTEEALTNIADVTLSNIYKLKS
GKVCENIVLPS

Sequences:

>Translated_331_residues
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKVLEKLAALGVKIVALRCAGFN
NVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRIHRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVM
RILKGFGMNILAYDPFKNPVVEELGGQYVELDELYAKSHVITLHCPATPENYHLLNRKAFAKMKDGVMIVNTSRGSLIDT
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLTEEALTNIADVTLSNIYKLKS
GKVCENIVLPS
>Mature_331_residues
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKVLEKLAALGVKIVALRCAGFN
NVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRIHRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVM
RILKGFGMNILAYDPFKNPVVEELGGQYVELDELYAKSHVITLHCPATPENYHLLNRKAFAKMKDGVMIVNTSRGSLIDT
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLTEEALTNIADVTLSNIYKLKS
GKVCENIVLPS

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=309, Percent_Identity=32.3624595469256, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI4557497, Length=309, Percent_Identity=32.3624595469256, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI145580578, Length=280, Percent_Identity=31.7857142857143, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557499, Length=280, Percent_Identity=31.7857142857143, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI145580575, Length=280, Percent_Identity=31.7857142857143, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI23308577, Length=212, Percent_Identity=33.9622641509434, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI6912396, Length=223, Percent_Identity=29.5964125560538, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1787645, Length=326, Percent_Identity=70.8588957055215, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI87082289, Length=286, Percent_Identity=29.3706293706294, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789279, Length=247, Percent_Identity=31.1740890688259, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788660, Length=210, Percent_Identity=27.1428571428571, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25147481, Length=280, Percent_Identity=30.3571428571429, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17532191, Length=238, Percent_Identity=28.1512605042017, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=235, Percent_Identity=34.8936170212766, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6320925, Length=235, Percent_Identity=34.0425531914894, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324055, Length=212, Percent_Identity=35.8490566037736, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324964, Length=216, Percent_Identity=26.8518518518519, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325144, Length=125, Percent_Identity=33.6, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321253, Length=189, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=27.1428571428571, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646446, Length=305, Percent_Identity=32.1311475409836, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24646448, Length=305, Percent_Identity=32.1311475409836, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24646452, Length=305, Percent_Identity=32.1311475409836, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24646450, Length=305, Percent_Identity=32.1311475409836, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI62472511, Length=305, Percent_Identity=32.1311475409836, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=30.2904564315353, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574286, Length=277, Percent_Identity=29.2418772563177, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45552429, Length=269, Percent_Identity=26.7657992565056, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585514, Length=269, Percent_Identity=26.7657992565056, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28574282, Length=269, Percent_Identity=26.7657992565056, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45551003, Length=269, Percent_Identity=26.7657992565056, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28574284, Length=269, Percent_Identity=26.7657992565056, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28571528, Length=275, Percent_Identity=26.5454545454545, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 37141; Mature: 37141

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKV
CEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHCCCEEEEEEECCCCCHHH
LEKLAALGVKIVALRCAGFNNVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRI
HHHHHHCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVMRILKGFGMNILAYDPFKNPV
HHHHHHHHHCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCHH
VEELGGQYVELDELYAKSHVITLHCPATPENYHLLNRKAFAKMKDGVMIVNTSRGSLIDT
HHHHCCCEEEHHHHHHCCEEEEEECCCCCCCCEEHHHHHHHHHCCCEEEEECCCCCEEHH
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLT
HHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEECCHHHHH
EEALTNIADVTLSNIYKLKSGKVCENIVLPS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKV
CEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHCCCEEEEEEECCCCCHHH
LEKLAALGVKIVALRCAGFNNVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRI
HHHHHHCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVMRILKGFGMNILAYDPFKNPV
HHHHHHHHHCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCHH
VEELGGQYVELDELYAKSHVITLHCPATPENYHLLNRKAFAKMKDGVMIVNTSRGSLIDT
HHHHCCCEEEHHHHHHCCEEEEEECCCCCCCCEEHHHHHHHHHCCCEEEEECCCCCEEHH
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLT
HHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEECCHHHHH
EEALTNIADVTLSNIYKLKSGKVCENIVLPS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]