Click here to switch to the map view.

The map label for this gene is nag3 [H]

Identifier: 148827205

GI number: 148827205

Start: 504656

End: 506416

Strand: Reverse

Name: nag3 [H]

Synonym: CGSHiGG_02750

Alternate gene names: 148827205

Gene position: 506416-504656 (Counterclockwise)

Preceding gene: 148827206

Following gene: 148827204

Centisome position: 26.83

GC content: 37.37

Gene sequence:

>1761_bases
ATGGCTGTTGATTTAAGTAAAAAACCATTTTATCTCAATGCTGAACAAATTGCTTGGGTAGAAAATACGATTGCAAATAT
GAGTTTAGAAGAAAAAATTGGGCAATTATTTGTCAATATGGGTTCAAGTCGTTCAGAAGAATATTTAACTAATATGGTTA
ATAAATATCATATTGGAGCAGTGCGTTATAACCCAGCTAAGGCTGAAGAGGTTTATGAACAAAATCATATTTTGCAAACG
AAGAGTAAAATTCCGCTTTTGATTGCGGCAAATACTGAAGCGGGCGGAAATGGTGCTTGTACAGATGGCACGGAAATTGG
TCTTCAAATAAAAATTGCTGCGACTCAAGATGCTCATTATGCCTATGAAATGGGCAGAGTAGCGGGGATTGAGGCTTCGG
CTATTGGTTGTAACTGGAGTTTTGCCCCAATTGTGGACATTAATTATAACTGGCGCAATCCTATTATTTCTAACCGCTCG
TTTAGTTCTGATCCTGATACCGTATTGGAAATGGCACTTGCTTATATGAAAGGCATTCAAGAAAGTGGTATTCAACCTTC
CGCTAAACATTTCCCAGGAGATGGTGTTGATGAGCGAGACCAGCATCTTTCCTTTAGCATTAATGGCTTTTCATGTGAAA
AATGGGATGAAACATTCGGTAAAGTTTATCAAGGGTTAATTGATGCAGGGTTGCCTTCTATAATGGCGGGGCATATTCAT
CTACCAAGTTATGAGAAATATTTTTCTCCAGAATTAAGTGATGAAGAGTGCTTGCCTGCTACACTTTCTAAACCGCTTTT
AACAGATTTATTGCGTGGAAAATTAGGGTTTAATGGTGTAATTGTTACTGATGCAAGCCATATGGTTGCAATGACATCAA
GCATGAAACGTAGTGAACTTGTTCCTCAAGCTATTGCTGCTGGTTGTGATTTATTCTTATTCTTCAATGATCCAGATGAA
GATTTTGCTTATATGATGGATGGATACCGCAATGGTGTCATTACTGAAACACGTTTATATGATGCTCTTTGCAGAATATT
AGGTTTTAAAGCGAAACTTAATTTGCATAAGACACCAAAAGAACAAATTCTACCTCCAAAAGAAGCTGCTATGGCAAAAA
TTGGACTGCCAGAAAATAAAGCGATTTTCCGTCAAGTTGCTGATAAAGCGATTACGCTTGTTAAAAATAAACAAGCAATT
TTCCCGATTTCGCCTGAAAAACACCCTCGAGTGTTATTAGTGAATATTAAAGGGGTTGAGGGAGGCTTTGGAAAAATGGT
CGCAGGAAGCTCAAAAAGTGCCATTGAAATTTTACAAGAGCAATTGACTAAAAAAGGTTTTTCCGTCTCAATTTATCAGT
CTCCGATGGATAATATTCTAGCTCTTCCCGATGAAGAACAAGTACAAGCGATTCGCAACGAATACTCCCAAAAACGTCCA
ATCTCTGATTTAGTTGGTCAATATGATTTGATTATTAATGTAGCAAATGTACATATGAGTACCGTACAGCGCATCGTGTG
GCAAGCAACTAAAGGCACGCCGGATATACCTTTCTATGTGCATGAAATTCCAACGATTTTTGTTTCTGTGCAATGTCCAT
TCCATTTGGCAGATGTTCCTCAAGTTAAAACTTACATTAATGCCTATGATGGAAAACAAGACACGATGGAAATGTTAGTA
GAAAAATTAATGGGCTATTCTGATTTTAAAGGCGTAAGCCCGGTTGATGCATATTGTGGGTTTAAAGATACTTATATTTA
A

Upstream 100 bases:

>100_bases
AAATTAGTTTATGGTCAATTTGATACGGGATACCAGCAGACATTGATTGAACAGTTCCACCATAATTTGAATACACAATC
AAATAATTAAGGAGACAAAT

Downstream 100 bases:

>100_bases
CCATACAAGGATAAATTTATGAACACATTAGAATCAAGACCTTTTGGTCTCAAAGATAAAATTGCCTATATGACAGGTGA
CATCGCAAATGATATGAGCT

Product: beta-hexosamidase A

Products: NA

Alternate protein names: 3-beta-N-acetyl-D-glucosaminidase/beta-D-glucosidase; Nag3 [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MAVDLSKKPFYLNAEQIAWVENTIANMSLEEKIGQLFVNMGSSRSEEYLTNMVNKYHIGAVRYNPAKAEEVYEQNHILQT
KSKIPLLIAANTEAGGNGACTDGTEIGLQIKIAATQDAHYAYEMGRVAGIEASAIGCNWSFAPIVDINYNWRNPIISNRS
FSSDPDTVLEMALAYMKGIQESGIQPSAKHFPGDGVDERDQHLSFSINGFSCEKWDETFGKVYQGLIDAGLPSIMAGHIH
LPSYEKYFSPELSDEECLPATLSKPLLTDLLRGKLGFNGVIVTDASHMVAMTSSMKRSELVPQAIAAGCDLFLFFNDPDE
DFAYMMDGYRNGVITETRLYDALCRILGFKAKLNLHKTPKEQILPPKEAAMAKIGLPENKAIFRQVADKAITLVKNKQAI
FPISPEKHPRVLLVNIKGVEGGFGKMVAGSSKSAIEILQEQLTKKGFSVSIYQSPMDNILALPDEEQVQAIRNEYSQKRP
ISDLVGQYDLIINVANVHMSTVQRIVWQATKGTPDIPFYVHEIPTIFVSVQCPFHLADVPQVKTYINAYDGKQDTMEMLV
EKLMGYSDFKGVSPVDAYCGFKDTYI

Sequences:

>Translated_586_residues
MAVDLSKKPFYLNAEQIAWVENTIANMSLEEKIGQLFVNMGSSRSEEYLTNMVNKYHIGAVRYNPAKAEEVYEQNHILQT
KSKIPLLIAANTEAGGNGACTDGTEIGLQIKIAATQDAHYAYEMGRVAGIEASAIGCNWSFAPIVDINYNWRNPIISNRS
FSSDPDTVLEMALAYMKGIQESGIQPSAKHFPGDGVDERDQHLSFSINGFSCEKWDETFGKVYQGLIDAGLPSIMAGHIH
LPSYEKYFSPELSDEECLPATLSKPLLTDLLRGKLGFNGVIVTDASHMVAMTSSMKRSELVPQAIAAGCDLFLFFNDPDE
DFAYMMDGYRNGVITETRLYDALCRILGFKAKLNLHKTPKEQILPPKEAAMAKIGLPENKAIFRQVADKAITLVKNKQAI
FPISPEKHPRVLLVNIKGVEGGFGKMVAGSSKSAIEILQEQLTKKGFSVSIYQSPMDNILALPDEEQVQAIRNEYSQKRP
ISDLVGQYDLIINVANVHMSTVQRIVWQATKGTPDIPFYVHEIPTIFVSVQCPFHLADVPQVKTYINAYDGKQDTMEMLV
EKLMGYSDFKGVSPVDAYCGFKDTYI
>Mature_585_residues
AVDLSKKPFYLNAEQIAWVENTIANMSLEEKIGQLFVNMGSSRSEEYLTNMVNKYHIGAVRYNPAKAEEVYEQNHILQTK
SKIPLLIAANTEAGGNGACTDGTEIGLQIKIAATQDAHYAYEMGRVAGIEASAIGCNWSFAPIVDINYNWRNPIISNRSF
SSDPDTVLEMALAYMKGIQESGIQPSAKHFPGDGVDERDQHLSFSINGFSCEKWDETFGKVYQGLIDAGLPSIMAGHIHL
PSYEKYFSPELSDEECLPATLSKPLLTDLLRGKLGFNGVIVTDASHMVAMTSSMKRSELVPQAIAAGCDLFLFFNDPDED
FAYMMDGYRNGVITETRLYDALCRILGFKAKLNLHKTPKEQILPPKEAAMAKIGLPENKAIFRQVADKAITLVKNKQAIF
PISPEKHPRVLLVNIKGVEGGFGKMVAGSSKSAIEILQEQLTKKGFSVSIYQSPMDNILALPDEEQVQAIRNEYSQKRPI
SDLVGQYDLIINVANVHMSTVQRIVWQATKGTPDIPFYVHEIPTIFVSVQCPFHLADVPQVKTYINAYDGKQDTMEMLVE
KLMGYSDFKGVSPVDAYCGFKDTYI

Specific function: Catalyzes the cleavage of beta-N-acetyl-D-glucosaminides and beta-D-glucosides. Might be involved in the degradation of glucuronic acid-containing glycosaminoglycans such as hyaluronic acid [H]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788453, Length=405, Percent_Identity=24.1975308641975, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787350, Length=261, Percent_Identity=27.2030651340996, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3 [H]

EC number: =3.2.1.21; =3.2.1.52 [H]

Molecular weight: Translated: 65021; Mature: 64890

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVDLSKKPFYLNAEQIAWVENTIANMSLEEKIGQLFVNMGSSRSEEYLTNMVNKYHIGA
CCCCCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEE
VRYNPAKAEEVYEQNHILQTKSKIPLLIAANTEAGGNGACTDGTEIGLQIKIAATQDAHY
EEECCHHHHHHHHHHCCEEECCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCHH
AYEMGRVAGIEASAIGCNWSFAPIVDINYNWRNPIISNRSFSSDPDTVLEMALAYMKGIQ
HHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ESGIQPSAKHFPGDGVDERDQHLSFSINGFSCEKWDETFGKVYQGLIDAGLPSIMAGHIH
HCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEE
LPSYEKYFSPELSDEECLPATLSKPLLTDLLRGKLGFNGVIVTDASHMVAMTSSMKRSEL
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHH
VPQAIAAGCDLFLFFNDPDEDFAYMMDGYRNGVITETRLYDALCRILGFKAKLNLHKTPK
HHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCEEECCCCCCH
EQILPPKEAAMAKIGLPENKAIFRQVADKAITLVKNKQAIFPISPEKHPRVLLVNIKGVE
HHCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHEECCCEEECCCCCCCCEEEEEEEECCC
GGFGKMVAGSSKSAIEILQEQLTKKGFSVSIYQSPMDNILALPDEEQVQAIRNEYSQKRP
CCCCCEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCC
ISDLVGQYDLIINVANVHMSTVQRIVWQATKGTPDIPFYVHEIPTIFVSVQCPFHLADVP
HHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEECCEEECCCC
QVKTYINAYDGKQDTMEMLVEKLMGYSDFKGVSPVDAYCGFKDTYI
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
AVDLSKKPFYLNAEQIAWVENTIANMSLEEKIGQLFVNMGSSRSEEYLTNMVNKYHIGA
CCCCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEE
VRYNPAKAEEVYEQNHILQTKSKIPLLIAANTEAGGNGACTDGTEIGLQIKIAATQDAHY
EEECCHHHHHHHHHHCCEEECCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCHH
AYEMGRVAGIEASAIGCNWSFAPIVDINYNWRNPIISNRSFSSDPDTVLEMALAYMKGIQ
HHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ESGIQPSAKHFPGDGVDERDQHLSFSINGFSCEKWDETFGKVYQGLIDAGLPSIMAGHIH
HCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEE
LPSYEKYFSPELSDEECLPATLSKPLLTDLLRGKLGFNGVIVTDASHMVAMTSSMKRSEL
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHH
VPQAIAAGCDLFLFFNDPDEDFAYMMDGYRNGVITETRLYDALCRILGFKAKLNLHKTPK
HHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCEEECCCCCCH
EQILPPKEAAMAKIGLPENKAIFRQVADKAITLVKNKQAIFPISPEKHPRVLLVNIKGVE
HHCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHEECCCEEECCCCCCCCEEEEEEEECCC
GGFGKMVAGSSKSAIEILQEQLTKKGFSVSIYQSPMDNILALPDEEQVQAIRNEYSQKRP
CCCCCEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCC
ISDLVGQYDLIINVANVHMSTVQRIVWQATKGTPDIPFYVHEIPTIFVSVQCPFHLADVP
HHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEECCEEECCCC
QVKTYINAYDGKQDTMEMLVEKLMGYSDFKGVSPVDAYCGFKDTYI
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA