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The map label for this gene is holD [H]

Identifier: 148827171

GI number: 148827171

Start: 464497

End: 464901

Strand: Reverse

Name: holD [H]

Synonym: CGSHiGG_02515

Alternate gene names: 148827171

Gene position: 464901-464497 (Counterclockwise)

Preceding gene: 148827173

Following gene: 148827170

Centisome position: 24.63

GC content: 40.74

Gene sequence:

>405_bases
ATGAACAGACGCGATCTTCTTTTACAAGAAATGGGCATTTCCCAGTGGGAATTATATCGCCCCGAGGTACTGCAAGGTTC
AGTAGGAATTAGTGTGGCAGAGAATATTCGCTTTATCACTGTTTCCGATGAAAATATCAGTAGCTCGCCTTTGTTGGCTG
ATGTGCTGTTAAGCCTTAATCTTAAAAAAGAAAATTGTTTATGTTTGAATTACGATCAAATCCAGCATATGGAATGTAAA
CAGCCTATTCGTTATTGGTTACTATCAGAAAATAGCGACCAAATTGACCGCACTTTGCCATTTTGCAAGCAGGCTGAGCA
GGTTTATCGCTCGCCAAGTTGGCAGCAATTTCAATCTAATCATCAAGCCAAACGAGCGTTGTGGCAACAAATTCAGCAGC
CTTAA

Upstream 100 bases:

>100_bases
GAATCCTGTGGGTTATTGGTTAAAATTACGCACCATTTTAGCAAAGATTAATAAAAGAAATTAACACTATTTTTCAAGCA
ACGTTTTTAAAAATTCACTT

Downstream 100 bases:

>100_bases
TTATGTCTATTATTTCTCAAATTGAAGCCTGTGATTTCGAGCGATTGTATGAAATCGAGCAGCAAGCGCATCTGGTGCCT
TGGTCGTTAGGGACGTTAAA

Product: DNA polymerase III subunit psi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRFITVSDENISSSPLLADVLLSLNLKKENCLCLNYDQIQHMECK
QPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSNHQAKRALWQQIQQP

Sequences:

>Translated_134_residues
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRFITVSDENISSSPLLADVLLSLNLKKENCLCLNYDQIQHMECK
QPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSNHQAKRALWQQIQQP
>Mature_134_residues
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRFITVSDENISSSPLLADVLLSLNLKKENCLCLNYDQIQHMECK
QPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSNHQAKRALWQQIQQP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The exact function of the psi subunit is unknown [H]

COG id: COG3050

COG function: function code L; DNA polymerase III, psi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790831, Length=131, Percent_Identity=32.0610687022901, Blast_Score=74, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004615
- InterPro:   IPR018382 [H]

Pfam domain/function: PF03603 DNA_III_psi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 15752; Mature: 15752

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRFITVSDENISSSPLLADVLLSLN
CCHHHHHHHHHCCCHHHHCCCHHHCCCCCCEEECCEEEEEEECCCCCCCHHHHHHHHHCC
LKKENCLCLNYDQIQHMECKQPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSN
CCCCCEEEECHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
HQAKRALWQQIQQP
HHHHHHHHHHHCCC
>Mature Secondary Structure
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRFITVSDENISSSPLLADVLLSLN
CCHHHHHHHHHCCCHHHHCCCHHHCCCCCCEEECCEEEEEEECCCCCCCHHHHHHHHHCC
LKKENCLCLNYDQIQHMECKQPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSN
CCCCCEEEECHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
HQAKRALWQQIQQP
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]