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The map label for this gene is argG [H]

Identifier: 148827144

GI number: 148827144

Start: 426158

End: 427492

Strand: Direct

Name: argG [H]

Synonym: CGSHiGG_02370

Alternate gene names: 148827144

Gene position: 426158-427492 (Clockwise)

Preceding gene: 148827143

Following gene: 148827151

Centisome position: 22.58

GC content: 39.93

Gene sequence:

>1335_bases
ATGTCAAATACTATTCTACAGAATTTACCTAAAGGACAAAAAGTAGGGATCGCTTTCTCTGGTGGTTTAGACACTAGTGC
AGCATTACTTTGGATGCGTCAAAAAGGCGCAGTGCCTTATGCCTACACCGCTAATTTAGGGCAACCCGATGAAGATGATT
ACAACGCAATCCCTAAAAAAGCCATGGCGTATGGTGCTGAAAATGCTCGCTTAATTGATTGTCGTGCTCAGCTTGCTCAT
GAAGGCATTGCCGCAATTCAATGTGGTGCATTCCATATTTCTACAGGCGGCATTCCTTACTTCAATACAACGCCTCTTGG
TCGCGCCGTAACAGGCACAATGCTTGTAGCAGCAATGAAAGAAGATGATGTAAACATTTGGGGCGACGGCTCCACTTTCA
AAGGGAATGATATTGAACGTTTCTATCGTTATGGTTTATTAACGAACCCGAATTTAAAAATCTATAAACCTTGGTTAGAT
GTACAATTTATTGAAGAACTTGGCGGTCGTTTAGAAATGTCTCAATTTCTTATTGAAAATGGTTTTGACTATAAAATGTC
CGTTGAAAAAGCCTATTCCACTGATTCCAATATGCTGGGTGCAACCCATGAAGCAAAAGACTTAGAACAATTAAGCACAG
GAATGAAAATTGTTAAGCCGATTATGGGTGTCGCATTTTGGGATGAAAAGGTTGAAATTAAACCTGAAACAGTCACAGTC
ACTTTTGAAGATGGCGTGCCTGTGGCATTAAATGGCAAACATTTTGATAATGCTGTTGATCTTATTCTAGAAGCCAATCG
TATTGGTGGGCGTCACGGTTTAGGAATGTCAGACCAAATCGAAAATCGTATTATTGAAGCCAAATCTCGTGGTATTTATG
AAGCACCAGGAATGGCATTATTGCATATCGCTTACGAACGTTTAGTCACAGGTATTCACAATGAAGATACCATCGAACAA
TATCGTATTAATGGCATTCGCTTAGGTCGTTTGTTATATCAAGGTCGTTGGTTTGATCCGCAAGCATTAATGTTGCGTGA
AACAGCACAACGTTGGGTGGCAAAAGCAATTACAGGCACAGTAACCCTTGAACTTCGTCGTGGTAACGACTTCACAATTT
TAAATACCGAATCGCCAAACTTAACCTACGAAGCAGAGCGTTTAAGTATGGAAAAAGTGGAAGATGCACCATTTGATCCA
ATTGATCGTATCGGTCAATTAACAATGCGTAATTTAGATGTATCAGACACCCGAGGTAAACTTGGCATCTATGCACAAAC
AGGTTTATTAAGCGCAATTAAAGATTCCGTATTGCCACAATTAGGCAAAAAATAA

Upstream 100 bases:

>100_bases
AAACACTAGACAAAGAGGAAAAGTGCGGTTATACTCTGTCGATTTTTTATTCGCAAAAATCGATTATTTATTCAATTCTA
ATTATTAGTAAGGAAAATAT

Downstream 100 bases:

>100_bases
ATAATAAAATCTGCATTTCAATGACTGATATTTAACATCATGAAATGCAGATTCTTTCACAAAATATTAGTTAGCTAAAA
AAACTAGAAATATAAGAAAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSNTILQNLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEDDYNAIPKKAMAYGAENARLIDCRAQLAH
EGIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLD
VQFIEELGGRLEMSQFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEQLSTGMKIVKPIMGVAFWDEKVEIKPETVTV
TFEDGVPVALNGKHFDNAVDLILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQ
YRINGIRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFDP
IDRIGQLTMRNLDVSDTRGKLGIYAQTGLLSAIKDSVLPQLGKK

Sequences:

>Translated_444_residues
MSNTILQNLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEDDYNAIPKKAMAYGAENARLIDCRAQLAH
EGIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLD
VQFIEELGGRLEMSQFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEQLSTGMKIVKPIMGVAFWDEKVEIKPETVTV
TFEDGVPVALNGKHFDNAVDLILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQ
YRINGIRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFDP
IDRIGQLTMRNLDVSDTRGKLGIYAQTGLLSAIKDSVLPQLGKK
>Mature_443_residues
SNTILQNLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEDDYNAIPKKAMAYGAENARLIDCRAQLAHE
GIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLDV
QFIEELGGRLEMSQFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEQLSTGMKIVKPIMGVAFWDEKVEIKPETVTVT
FEDGVPVALNGKHFDNAVDLILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQY
RINGIRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFDPI
DRIGQLTMRNLDVSDTRGKLGIYAQTGLLSAIKDSVLPQLGKK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=408, Percent_Identity=27.9411764705882, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI16950633, Length=408, Percent_Identity=27.9411764705882, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1789563, Length=438, Percent_Identity=79.2237442922374, Blast_Score=717, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=420, Percent_Identity=28.0952380952381, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21358151, Length=396, Percent_Identity=27.5252525252525, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 49290; Mature: 49159

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTILQNLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEDDYNAIPKK
CCCCHHHHCCCCCEEEEEEECCCCCHHHEEEEHHCCCCCEEEECCCCCCCCCCHHHCCHH
AMAYGAENARLIDCRAQLAHEGIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMK
HHHCCCCCCEEEHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCCCHHHHHHEEEEEEC
EDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLDVQFIEELGGRLEMSQFLIEN
CCCCEEEECCCCCCCCCHHHHHHHCCEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHHC
GFDYKMSVEKAYSTDSNMLGATHEAKDLEQLSTGMKIVKPIMGVAFWDEKVEIKPETVTV
CCCEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCEEEE
TFEDGVPVALNGKHFDNAVDLILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMAL
EECCCCCEEECCCCCCCHHHHEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
LHIAYERLVTGIHNEDTIEQYRINGIRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGT
HHHHHHHHHHCCCCCCHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEE
VTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFDPIDRIGQLTMRNLDVSDTRGK
EEEEEECCCCEEEEECCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCC
LGIYAQTGLLSAIKDSVLPQLGKK
EEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNTILQNLPKGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEDDYNAIPKK
CCCHHHHCCCCCEEEEEEECCCCCHHHEEEEHHCCCCCEEEECCCCCCCCCCHHHCCHH
AMAYGAENARLIDCRAQLAHEGIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMK
HHHCCCCCCEEEHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCCCHHHHHHEEEEEEC
EDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLKIYKPWLDVQFIEELGGRLEMSQFLIEN
CCCCEEEECCCCCCCCCHHHHHHHCCEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHHC
GFDYKMSVEKAYSTDSNMLGATHEAKDLEQLSTGMKIVKPIMGVAFWDEKVEIKPETVTV
CCCEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCEEEE
TFEDGVPVALNGKHFDNAVDLILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMAL
EECCCCCEEECCCCCCCHHHHEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
LHIAYERLVTGIHNEDTIEQYRINGIRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGT
HHHHHHHHHHCCCCCCHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEE
VTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFDPIDRIGQLTMRNLDVSDTRGK
EEEEEECCCCEEEEECCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCC
LGIYAQTGLLSAIKDSVLPQLGKK
EEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA