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The map label for this gene is prfC [H]

Identifier: 148827151

GI number: 148827151

Start: 434939

End: 436522

Strand: Direct

Name: prfC [H]

Synonym: CGSHiGG_02415

Alternate gene names: 148827151

Gene position: 434939-436522 (Clockwise)

Preceding gene: 148827144

Following gene: 148827161

Centisome position: 23.05

GC content: 41.86

Gene sequence:

>1584_bases
ATGAGTTATCCATTAGAAGAAGTTAATAAACGTCGCACTTTTGCGATTATTTCCCACCCTGATGCGGGTAAAACAACGAT
TACTGAAAAAGTACTTTTATACGGCAATGCAATTCAAACTGCAGGTTCTGTAAAAGGCAAAGGCTCGGCGGCTCACGCGA
AATCTGACTGGATGGAAATGGAAAAACAACGTGGGATTTCGATTACCACTTCTGTGATGCAATTTCCTTATAATGATTGT
TTAGTGAATTTGCTCGATACCCCCGGACACGAAGATTTCTCTGAAGATACTTACCGCACTTTAACCGCTGTGGATAGCTG
TTTGATGGTTATTGACTCTGCCAAAGGGGTGGAGGAACGTACGATTAAGTTGATGGAAGTCACACGTTTACGTGATACGC
CCATTATTACGTTTATGAATAAACTCGACCGTGATATTCGTGATCCGATGGAATTGTTGGACGAGGTGGAAAATGTGTTG
AAAATTCGTTGTGCGCCAATTACTTGGCCAATTGGCTGTGGGAAGTTGTTTAAGGGCGTTTATCATCTTGCAAAAGATGA
AACCTATTTATATCAATCAGGGCAGGGTTCAACCATTCAGGCAGTGCGTGTTGTTAAAGGCTTGAATAATCCTGAATTAG
ATGTTGCTGTTGGCGATGATTTAGCACAACAGCTTCGTGATGAATTGGAATTGGTGCAAGGCGCAAGTAATGAATTTGAG
CAAGATGCCTTTATTAAGGGCGAGCTTACCCCTGTATTTTTCGGGACAGCATTAGGCAATTTTGGGGTGGATCATTTCCT
TGATGGCTTAACACAATGGGCTCCGAAACCGCAATCTCGTCAAGCGGATACAAGAACGGTGGAAAGTGCGGAAGAAAAAT
TCAGTGGTTTTGTGTTTAAAATTCAAGCCAATATGGATCCGAAACACCGTGATAGAGTGGCGTTTATGCGTGTGGTTTCG
GGCAAATATGAAAAAGGGATGAAACTTAAACACGTCCGAATTGGTAAAGATGTGGTGATTTCTGACGCACTCACCTTTAT
GGCTGGCGACCGTGCTCACGCCGAAGAAGCTTACGCTGGTGATATTATTGGTTTACATAATCACGGTACGATTCAAATTG
GCGATACGTTCACGCAAGGCGAAACGCTGAAATTTACAGGCATTCCAAATTTTGCGCCAGAGTTGTTCCGTCGTATTCGT
TTGAAAGATCCTCTTAAACAAAAACAACTGTTAAAAGGCTTGGTGCAACTTTCTGAAGAGGGTGCAGTGCAAGTGTTTCG
TCCATTATTAAACAACGATTTAATTGTGGGGGCTGTGGGTGTGTTGCAGTTTGATGTGGTGGTTTCGCGTTTAAAAACTG
AATATAACGTGGAAGCGATTTATGAAAATGTGAACGTTGCCACGGCTCGTTGGGTGGAATGTGCCGATGGGAAAAAATTT
GAAGAATTTAAACGTAAAAACGAGCAAAATTTAGCCTTAGATGGCGGCGATAATTTAACCTATATTGCGCCAACAATGGT
AAATCTAAATTTAGCTCAAGAACGTTATCCTGATGTCGTTTTTTACAAAACTCGCGAACATTAA

Upstream 100 bases:

>100_bases
TTCTAAGAAAACCAACCGCACTTTATTTGCAAATTCGTTTGAGCAAAAACCTAAAATCAGGGAAAATAACCCACATTTTT
AATTGAACAAAGAAGAAACA

Downstream 100 bases:

>100_bases
AATGACAAAGTGCGGTAAAATTCTACCGCACTTTTTCAGAAAAAAAGCGTTGAAATTTAATTCAACGCTTTTCTTTTATT
TAGAGAGATTTTCTTGTTTT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MSYPLEEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSAAHAKSDWMEMEKQRGISITTSVMQFPYNDC
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIITFMNKLDRDIRDPMELLDEVENVL
KIRCAPITWPIGCGKLFKGVYHLAKDETYLYQSGQGSTIQAVRVVKGLNNPELDVAVGDDLAQQLRDELELVQGASNEFE
QDAFIKGELTPVFFGTALGNFGVDHFLDGLTQWAPKPQSRQADTRTVESAEEKFSGFVFKIQANMDPKHRDRVAFMRVVS
GKYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAGDIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELFRRIR
LKDPLKQKQLLKGLVQLSEEGAVQVFRPLLNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYENVNVATARWVECADGKKF
EEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVVFYKTREH

Sequences:

>Translated_527_residues
MSYPLEEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSAAHAKSDWMEMEKQRGISITTSVMQFPYNDC
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIITFMNKLDRDIRDPMELLDEVENVL
KIRCAPITWPIGCGKLFKGVYHLAKDETYLYQSGQGSTIQAVRVVKGLNNPELDVAVGDDLAQQLRDELELVQGASNEFE
QDAFIKGELTPVFFGTALGNFGVDHFLDGLTQWAPKPQSRQADTRTVESAEEKFSGFVFKIQANMDPKHRDRVAFMRVVS
GKYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAGDIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELFRRIR
LKDPLKQKQLLKGLVQLSEEGAVQVFRPLLNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYENVNVATARWVECADGKKF
EEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVVFYKTREH
>Mature_526_residues
SYPLEEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSAAHAKSDWMEMEKQRGISITTSVMQFPYNDCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIITFMNKLDRDIRDPMELLDEVENVLK
IRCAPITWPIGCGKLFKGVYHLAKDETYLYQSGQGSTIQAVRVVKGLNNPELDVAVGDDLAQQLRDELELVQGASNEFEQ
DAFIKGELTPVFFGTALGNFGVDHFLDGLTQWAPKPQSRQADTRTVESAEEKFSGFVFKIQANMDPKHRDRVAFMRVVSG
KYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAGDIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELFRRIRL
KDPLKQKQLLKGLVQLSEEGAVQVFRPLLNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYENVNVATARWVECADGKKFE
EFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVVFYKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=500, Percent_Identity=28, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.2745098039216, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=28.4263959390863, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=26.5560165975104, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI157426893, Length=178, Percent_Identity=29.2134831460674, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI94966754, Length=158, Percent_Identity=29.1139240506329, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=86.3377609108159, Blast_Score=962, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=507, Percent_Identity=27.2189349112426, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1788922, Length=168, Percent_Identity=30.952380952381, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI48994988, Length=194, Percent_Identity=30.9278350515464, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=519, Percent_Identity=27.3603082851638, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=163, Percent_Identity=32.5153374233129, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=30.3703703703704, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=26.3485477178423, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=426, Percent_Identity=26.5258215962441, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=161, Percent_Identity=26.7080745341615, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=161, Percent_Identity=27.9503105590062, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=161, Percent_Identity=27.9503105590062, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582462, Length=492, Percent_Identity=26.8292682926829, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=23.0326295585413, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI78706572, Length=163, Percent_Identity=28.8343558282209, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59107; Mature: 58976

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYPLEEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSAAHAKSDWMEM
CCCCHHHHCCCCEEEEEECCCCCCCHHHHHHEEECCHHHCCCCCCCCCCCCCCHHHHHHH
EKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEER
HHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TIKLMEVTRLRDTPIITFMNKLDRDIRDPMELLDEVENVLKIRCAPITWPIGCGKLFKGV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHH
YHLAKDETYLYQSGQGSTIQAVRVVKGLNNPELDVAVGDDLAQQLRDELELVQGASNEFE
HHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCC
QDAFIKGELTPVFFGTALGNFGVDHFLDGLTQWAPKPQSRQADTRTVESAEEKFSGFVFK
CCCEEECCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEE
IQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAG
EECCCCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHCC
DIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLSEE
CEEEECCCCEEEECCEECCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
GAVQVFRPLLNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYENVNVATARWVECADGKKF
CHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEHHHEEECCCCCHH
EEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVVFYKTREH
HHHHHCCCCCEEEECCCCEEEEECEEEEEEEHHHHCCCEEEEECCCC
>Mature Secondary Structure 
SYPLEEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSAAHAKSDWMEM
CCCHHHHCCCCEEEEEECCCCCCCHHHHHHEEECCHHHCCCCCCCCCCCCCCHHHHHHH
EKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEER
HHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TIKLMEVTRLRDTPIITFMNKLDRDIRDPMELLDEVENVLKIRCAPITWPIGCGKLFKGV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHH
YHLAKDETYLYQSGQGSTIQAVRVVKGLNNPELDVAVGDDLAQQLRDELELVQGASNEFE
HHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCC
QDAFIKGELTPVFFGTALGNFGVDHFLDGLTQWAPKPQSRQADTRTVESAEEKFSGFVFK
CCCEEECCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEE
IQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAG
EECCCCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHCC
DIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLSEE
CEEEECCCCEEEECCEECCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
GAVQVFRPLLNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYENVNVATARWVECADGKKF
CHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEHHHEEECCCCCHH
EEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVVFYKTREH
HHHHHCCCCCEEEECCCCEEEEECEEEEEEEHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA