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The map label for this gene is nth [H]

Identifier: 148827112

GI number: 148827112

Start: 388504

End: 389139

Strand: Direct

Name: nth [H]

Synonym: CGSHiGG_02185

Alternate gene names: 148827112

Gene position: 388504-389139 (Clockwise)

Preceding gene: 148827111

Following gene: 148827113

Centisome position: 20.59

GC content: 38.99

Gene sequence:

>636_bases
ATGAACAAAACAAAAAGAATCGAAATACTCACAAGATTACGAGAGCAAAACCCCCACCCGACCACAGAATTACAGTATAA
TTCGCCCTTTGAATTATTAATTGCGGTCATTCTATCGGCGCAAGCAACCGATAAAGGCGTGAATAAAGCAACAGAAAAAC
TATTCCCCGTGGCAAACACGCCTCAAGCGATTTTAGATTTAGGATTAGACGGTTTAAAAAGCTATATTAAAACTATCGGG
CTTTTTAATAGCAAAGCAGAAAATATTATCAAAACTTGCCGAGATTTAATCGAAAAGCACAACGGCGAAATCCCCGAAAA
TCGTGAAGCCTTAGAAGCATTAGCAGGTGTCGGACGAAAAACAGCTAACGTGGTTTTAAACACAGCATTTGGTCATCCAA
CTATTGCGGTGGATACCCATATTTTCCGTGTATGCAATCGCACTAATTTTGCAGCGGGTAAAGATGTGGTAAAAGTGGAA
GAAAAGCTGCTTAAAGTCGTACCTAATGAATTTAAAGTGGATGTTCATCATTGGCTGATTTTACACGGACGCTACACTTG
TATCGCGCGTAAACCACGTTGCGGATCTTGCATTATCGAAGATCTTTGTGAATATAAAGAAAAAGTTGAATTTTAA

Upstream 100 bases:

>100_bases
CCCTGCTTGCGGGGGAAAGTGTCACAAAGTGACGGAAGGGGCTTACAGTCTTCTAAACAGATAATTCCAATAAATTAAAT
AGGCTAATTTCTACAAAAAT

Downstream 100 bases:

>100_bases
TTTTGCGGTAAAACAGCCTCATTATTTACCGCACTTTTTATTAAAAATAGGATAAAAAATGACAACAAATAATAAACAGC
GACAAACTTGGTCTAGCCGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKSYIKTIG
LFNSKAENIIKTCRDLIEKHNGEIPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVE
EKLLKVVPNEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVEF

Sequences:

>Translated_211_residues
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKSYIKTIG
LFNSKAENIIKTCRDLIEKHNGEIPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVE
EKLLKVVPNEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVEF
>Mature_211_residues
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKSYIKTIG
LFNSKAENIIKTCRDLIEKHNGEIPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVE
EKLLKVVPNEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVEF

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=91, Evalue=8e-19,
Organism=Escherichia coli, GI1787920, Length=211, Percent_Identity=82.9383886255924, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=30.8571428571429, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=26.8421052631579, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=29.9465240641711, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23730; Mature: 23730

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANT
CCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQAILDLGLDGLKSYIKTIGLFNSKAENIIKTCRDLIEKHNGEIPENREALEALAGVGRK
HHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
TANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVEEKLLKVVPNEFKVDVHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHEEHHHHEEE
LHGRYTCIARKPRCGSCIIEDLCEYKEKVEF
EECCEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANT
CCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQAILDLGLDGLKSYIKTIGLFNSKAENIIKTCRDLIEKHNGEIPENREALEALAGVGRK
HHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
TANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVEEKLLKVVPNEFKVDVHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHEEHHHHEEE
LHGRYTCIARKPRCGSCIIEDLCEYKEKVEF
EECCEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]