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The map label for this gene is aceF [H]

Identifier: 148827058

GI number: 148827058

Start: 324613

End: 326274

Strand: Direct

Name: aceF [H]

Synonym: CGSHiGG_01845

Alternate gene names: 148827058

Gene position: 324613-326274 (Clockwise)

Preceding gene: 148827057

Following gene: 148827059

Centisome position: 17.2

GC content: 41.64

Gene sequence:

>1662_bases
ATGTCAAAACAAATTCAAATTCCTGATATTGGTAGTGATGAAGTTACCGTAACAGAAGTGATGGTAAACGTAGGCGATAC
CATTTCTGTTGATCAATCTATCATTAACGTTGAAGGCGATAAAGCTTCAATGGAAGTGCCTGCACCAGAAGCGGGTGTTG
TAAAAGAAATTTTAGTCAAAGTGGGCGATAAAGTTTCAACTGGTACTCCAATGCTTGTATTAGAAGCGGCAGGTGCTGCT
CCAGCAGCTGATGAGCCTACTGCTCCAGTAGCTGATGCGCCTACTGCACCAGTGGTTGCTACCGCACCAACGGCTTCAGC
TATTGTAGAAGTAAATGTGCCAGATATTGGTGGTGATGAAGTCAATGTAACCGAAATTATGGTTGCAGTTGGCGATACAA
TCACTGAAGAACAATCTTTAATTACCGTTGAAGGCGATAAAGCCTCAATGGAAGTTCCTGCACCATTCGGCGGTGTGGTA
AAAGAAATTTTAGTGAAATCAGGCGATAAAGTTTCAACTGGTTCATTAATTATGCGTTTTGAAGTACTAGGTGCAGCTCC
AGCAGAATCAGCTTCAGCTTCAACATCAGCACCACAGGCAGCAGCTCCTGCTACTACTGCACAAGCACCACAGGCAGCAG
CTCCTGATACTACTGCACAAGCAGCTCAATCAAATAACAACGTATCTGGATTAAGCCAAGAACAAGTAGAAGCAAGTACA
GGATATGCACACGCAACACCAGTTATTCGTCGTTTAGCTCGTGAATTTGGTGTAAATCTAGATAAAGTAAAAGGTACTGG
TCGTAAAGGTCGTATCGTTAAAGAAGATATTGAAGCTTATGTGAAGACCGCAGTTAAAGCTTATGAAAGCGGTGCAACAG
CACAAGCTACTGGCAATGGTGTAGCAAATGGTGCTGGATTAGGCTTATTGCCATGGCCAAAAGTTGATTTCAGTAAATTT
GGCGAAATTGAAGAAGTTGAATTAAGTCGTATCAACAAAATTTCAGGTGCTAACTTACATCGTAACTGGGTAATAATTCC
ACACGTTACTCACTTCGATAAAGCAGATATCACGGATTTAGAAGCATTCCGTAAAGAACAAAATGCGTTAGCAGAAAAAC
AAAAACTTGGTGTAAAAATCACGCCAGTTGTGTTCATTATGAAAGCGGTGGCAAAAGCATTAGAAGCGTATCCACGTTTC
AACAGCTCAATTACCGAAGATGCTCAACGCTTAATCCTGAAAAAATATATTAACATCGGTGTCGCAGTAGATACGCCGAA
TGGCTTAGTAGTGCCTGTATTCAAAAATGTGAACAAGAAAGGCATTATCGAACTTTCACGTGAATTAATGGAAGTATCGA
AAAAAGCCCGTGAAGGCAAATTGACTGCATCAGATATGCAAGGTGGCTGTTTTACTATTTCAAGTCTTGGTGGTATTGGT
ACAACGCACTTTGCGCCAATCGTAAACGCACCAGAAGTTGCCATCCTTGGTGTGTCTAAATCATCGATGGAACCAGTATG
GAATGGTAAAGAATTTGCGCCACGCTTAATTCTTCCAATGTCATTATCATTCGACCACCGTGTCATTGATGGTGCAGATG
GTGCTCGTTTCATTAGCTATCTTGGCTCGGTTTTAGCAGATTTACGTCGCTTAGTAATGTAA

Upstream 100 bases:

>100_bases
GTTTGAATGTAGATCGTATCAATCCGCTTTATGCGTAATTGATGAAAACTGCGGTCAAAAAACATTGTGTTTTATGACCG
CACTTTAAAAGGAACAAAAA

Downstream 100 bases:

>100_bases
TCTGTAGGTGGGCTTTATGCCCACCGATTAATGAAATCTTTGGTGGGCAGAAACCCATCCTATAAAGACCGTAAAAGTGC
GGTCAATATTTTGAACGTTT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MSKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVKVGDKVSTGTPMLVLEAAGAA
PAADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDEVNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVV
KEILVKSGDKVSTGSLIMRFEVLGAAPAESASASTSAPQAAAPATTAQAPQAAAPDTTAQAAQSNNNVSGLSQEQVEAST
GYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVKAYESGATAQATGNGVANGAGLGLLPWPKVDFSKF
GEIEEVELSRINKISGANLHRNWVIIPHVTHFDKADITDLEAFRKEQNALAEKQKLGVKITPVVFIMKAVAKALEAYPRF
NSSITEDAQRLILKKYINIGVAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIG
TTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDHRVIDGADGARFISYLGSVLADLRRLVM

Sequences:

>Translated_553_residues
MSKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVKVGDKVSTGTPMLVLEAAGAA
PAADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDEVNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVV
KEILVKSGDKVSTGSLIMRFEVLGAAPAESASASTSAPQAAAPATTAQAPQAAAPDTTAQAAQSNNNVSGLSQEQVEAST
GYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVKAYESGATAQATGNGVANGAGLGLLPWPKVDFSKF
GEIEEVELSRINKISGANLHRNWVIIPHVTHFDKADITDLEAFRKEQNALAEKQKLGVKITPVVFIMKAVAKALEAYPRF
NSSITEDAQRLILKKYINIGVAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIG
TTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDHRVIDGADGARFISYLGSVLADLRRLVM
>Mature_552_residues
SKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVKVGDKVSTGTPMLVLEAAGAAP
AADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDEVNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVVK
EILVKSGDKVSTGSLIMRFEVLGAAPAESASASTSAPQAAAPATTAQAPQAAAPDTTAQAAQSNNNVSGLSQEQVEASTG
YAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVKAYESGATAQATGNGVANGAGLGLLPWPKVDFSKFG
EIEEVELSRINKISGANLHRNWVIIPHVTHFDKADITDLEAFRKEQNALAEKQKLGVKITPVVFIMKAVAKALEAYPRFN
SSITEDAQRLILKKYINIGVAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGT
THFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDHRVIDGADGARFISYLGSVLADLRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=482, Percent_Identity=28.6307053941909, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI31711992, Length=307, Percent_Identity=32.5732899022801, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI19923748, Length=206, Percent_Identity=34.4660194174757, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=24.6153846153846, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI203098753, Length=238, Percent_Identity=31.9327731092437, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI260898739, Length=143, Percent_Identity=35.6643356643357, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1786305, Length=549, Percent_Identity=68.6703096539162, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=28.9411764705882, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=439, Percent_Identity=29.3849658314351, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=29.2517006802721, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=209, Percent_Identity=35.4066985645933, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=29.5238095238095, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=434, Percent_Identity=27.1889400921659, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324258, Length=441, Percent_Identity=27.6643990929705, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=30.2752293577982, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=34.4186046511628, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=30.2127659574468, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=30.2127659574468, Blast_Score=119, Evalue=8e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 58005; Mature: 57874

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVK
CCCCCCCCCCCCCCEEHHHHHHHCCCCEECCCEEEEECCCCCEEECCCCCHHHHHHHHHH
VGDKVSTGTPMLVLEAAGAAPAADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDE
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCC
VNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVVKEILVKSGDKVSTGSLIMRF
CCHHHEEEHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHCCCCCCCCCEEEEE
EVLGAAPAESASASTSAPQAAAPATTAQAPQAAAPDTTAQAAQSNNNVSGLSQEQVEAST
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHC
GYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVKAYESGATAQATGNG
CCHHHHHHHHHHHHHHCCCEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCC
VANGAGLGLLPWPKVDFSKFGEIEEVELSRINKISGANLHRNWVIIPHVTHFDKADITDL
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHH
EAFRKEQNALAEKQKLGVKITPVVFIMKAVAKALEAYPRFNSSITEDAQRLILKKYINIG
HHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCE
VAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIG
EEEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
TTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDHRVIDGADGARFISY
CHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCHHHHHHH
LGSVLADLRRLVM
HHHHHHHHHHHHC
>Mature Secondary Structure 
SKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVK
CCCCCCCCCCCCCEEHHHHHHHCCCCEECCCEEEEECCCCCEEECCCCCHHHHHHHHHH
VGDKVSTGTPMLVLEAAGAAPAADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDE
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCC
VNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVVKEILVKSGDKVSTGSLIMRF
CCHHHEEEHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHCCCCCCCCCEEEEE
EVLGAAPAESASASTSAPQAAAPATTAQAPQAAAPDTTAQAAQSNNNVSGLSQEQVEAST
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHC
GYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVKAYESGATAQATGNG
CCHHHHHHHHHHHHHHCCCEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCC
VANGAGLGLLPWPKVDFSKFGEIEEVELSRINKISGANLHRNWVIIPHVTHFDKADITDL
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHH
EAFRKEQNALAEKQKLGVKITPVVFIMKAVAKALEAYPRFNSSITEDAQRLILKKYINIG
HHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCE
VAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIG
EEEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
TTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDHRVIDGADGARFISY
CHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCHHHHHHH
LGSVLADLRRLVM
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]